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Green Process for Producing Xylooligosaccharides by Using Sequential Auto-hydrolysis and Xylanase Hydrolysis
Appl Biochem Biotechnol. 2023 Dec 29. doi: 10.1007/s12010-023-04800-7. Online ahead of print.ABSTRACTXylooligosaccharides (XOS), as prebiotic oligomers, are increasingly receiving attention as high value-added products produced from lignocellulosic biomass. Although the XOS contains a series of different degrees of polymerization (DP) of xylose units, DP 2 and 3 (xylobiose (X2) and xylotriose (X3)) are regarded as the main active components in food and pharmaceutical fields. Therefore, in the study, in order to achieve the maximum production of XOS with the desired DP, a combination strategy of sequential auto-hydrolysis a...
Source: Applied Biochemistry and Biotechnology - December 29, 2023 Category: Biochemistry Authors: Yujie Zhai Lei Zhang Shuangquan Yao Xin Zhou Kankan Jiang Source Type: research

NDRG2 Promotes Lens Epithelial Cells Senescence via NLRP3/Caspase1-Mediated Pyroptosis
CONCLUSION: Our study confirmed downregulation of NDRG2 significantly inhibited UVB radiation-induced cell senescence by regulating caspase-1/NLRP3-mediated pyroptosis.PMID:38158484 | DOI:10.1007/s12010-023-04801-6 (Source: Applied Biochemistry and Biotechnology)
Source: Applied Biochemistry and Biotechnology - December 29, 2023 Category: Biochemistry Authors: Huilan Zhang Source Type: research

Myricetin Attenuates Ethylene Glycol-Induced Nephrolithiasis in Rats via Mitigating Oxidative Stress and Inflammatory Markers
Appl Biochem Biotechnol. 2023 Dec 30. doi: 10.1007/s12010-023-04831-0. Online ahead of print.ABSTRACTUrolithiasis or nephrolithiasis is a condition of kidney stone formation and is considered a painful disease of the urinary tract system. In this work, we planned to discover the therapeutic roles of myricetin on the ethylene glycol (EG)-induced nephrolithiasis in rats. The experimental rats were treated with 0.75% of EG through drinking water for 4 weeks to initiate the nephrolithiasis and subsequently treated with 25 and 50 mg/kg of myricetin. The body weight and urine volume were measured regularly. After the sacrificati...
Source: Applied Biochemistry and Biotechnology - December 29, 2023 Category: Biochemistry Authors: Xiaojie Yang Pei Zhang Jing Jiang Hesham S Almoallim Sulaiman Ali Alharbi Youfang Li Source Type: research

Occurrence Characteristics and Ecotoxic Effects of Microplastics in Environmental Media: a Mini Review
Appl Biochem Biotechnol. 2023 Dec 30. doi: 10.1007/s12010-023-04832-z. Online ahead of print.ABSTRACTThe issue of environmental pollution caused by the widespread presence of microplastics (MPs) in environmental media has garnered significant attention. However, research on MPs pollution has mainly focused on aquatic ecosystems in recent years. The sources and pollution characteristics of MPs in the environment, especially in solid waste, have not been well-described. Additionally, there are few reports on the ecotoxicity of MPs, which highlights the need to fill this gap. This review first summarizes the occurrence charac...
Source: Applied Biochemistry and Biotechnology - December 29, 2023 Category: Biochemistry Authors: Yuxin Wang Zhou Fu Dezheng Guan Jianwei Zhao Qi Zhang Qingxin Liu Jingliang Xie Yingjie Sun Liang Guo Source Type: research

Valorization of Cheese Whey Powder by Two-Step Fermentation for Gluconic Acid and Ethanol Preparation
Appl Biochem Biotechnol. 2023 Dec 30. doi: 10.1007/s12010-023-04834-x. Online ahead of print.ABSTRACTWhey from cheesemaking is an environmental contaminant with a high biochemical oxygen demand (BOD), containing an abundance of lactose. Hence, it has the potential to be utilized in the manufacturing of bio-based chemicals that have increased value. A designed sequential fermentation approach was employed in this research to convert enzymatic hydrolysate of cheese whey (primarily consists of glucose and galactose) into gluconic acid and bio-ethanol. This conversion was achieved by utilizing Gluconobacter oxydans and Sacchar...
Source: Applied Biochemistry and Biotechnology - December 29, 2023 Category: Biochemistry Authors: Rui Zhang Fan Li Xinlu Liu Xin Zhou Kankan Jiang Source Type: research

Unravelling the Untapped Pharmacological Potential of Plant Molecules as Inhibitors of BACE1: In Silico Explorations for Alzheimer's Disease
Appl Biochem Biotechnol. 2023 Dec 30. doi: 10.1007/s12010-023-04803-4. Online ahead of print.ABSTRACTAlzheimer's disease (AD) is an extremely complex, heterogeneous, and multifactorial neurodegenerative disease clinically characterized by progressive memory loss and progressive decline in cognitive function. There is currently no effective treatment for the onset and/or progression of the pathophysiological diseases of AD. The global prevalence of this disease has increased in recent years due to modern lifestyle. Therefore, there is an urgent need to develop a drug with significant neuroprotective potential. Since plant m...
Source: Applied Biochemistry and Biotechnology - December 29, 2023 Category: Biochemistry Authors: Karunanithi Kalaimathi Srinivasan Prabhu Muniappan Ayyanar Muthu Thiruvengadam Kadaikunnan Shine Sitrarasu Vijaya Prabhu Singamoorthy Amalraj Source Type: research

Sphincter of Oddi Dysfunction Induces Gallstone by Inhibiting the Expression of ABCB11 via PKC- α
This study aims to investigate it through in vitro and in vivo experiments. A mouse model of Oddi sphincter dysfunction was constructed by stone-inducing diet. We compared the two groups with PKC-α inhibitor GÖ6976 and PKC-α agonist thymeleatoxin. Oddi sphincter pressure and total bile duct pressure were measured. Biochemical analysis of total cholesterol, bile acid and bilirubin was then conducted. The histopathologic changes of bile duct were observed by HE staining and the ultrastructure of liver cells and surrounding tissues was observed by transmission electron microscopy. Through the above experiments, we found th...
Source: Applied Biochemistry and Biotechnology - December 29, 2023 Category: Biochemistry Authors: Lichao Zhu Wei Su Xianwen Xu Shuai Shao Chuan Qin Ruxin Gao Xinxing Wang Mingze Ma Junlin Gao Zhenhai Zhang Source Type: research

Preparation, Structural Characterization, and Enzymatic Properties of Alginate Lyase Immobilized on Magnetic Chitosan Microspheres
Appl Biochem Biotechnol. 2023 Dec 30. doi: 10.1007/s12010-023-04824-z. Online ahead of print.ABSTRACTAlginate lyase is an enzyme that catalyses the hydrolysis of alginate into alginate oligoalginates. To enhance enzyme stability and recovery, a facile strategy for alginate lyase immobilization was developed. Novel magnetic chitosan microspheres were synthesized and used as carriers to immobilize alginate lyase. The immobilization of alginate lyase on magnetic chitosan microspheres was successful, as proven by Fourier transform infrared spectroscopy and X-ray diffraction spectra. Enzyme immobilization exhibited the best per...
Source: Applied Biochemistry and Biotechnology - December 29, 2023 Category: Biochemistry Authors: Jinmeng Li Feng Yan Bingbing Huang Mengyan Zhang Xiaodan Wu Yuhuan Liu Roger Ruan Hongli Zheng Source Type: research

MiR-30c-5p-Targeted Regulation of GNAI2 Improves Neural Function Injury and Inflammation in Cerebral Ischemia-Reperfusion Injury
This study focused on miR-30c-5p in the disease. An oxygen-glucose deprivation/re-oxygenation (OGD/R) model was prepared in HT22 cells and transfected to overexpress miR-30c-5p and G Protein Subunit Alpha I2 (GNAI2) respectively or co-transfected to silence miR-30c-5p and GNAI2. Meanwhile, a middle cerebral artery occlusion (MCAO) model was constructed in mice, and miR-30c-5p and GNAI2 were silenced in vivo simultaneously. The mice were evaluated for neurological damage, apoptosis, and inflammation. HT22 cells were tested for cytotoxicity, proliferation, apoptosis, and inflammatory factors. The interaction between miR-30c-...
Source: Applied Biochemistry and Biotechnology - December 28, 2023 Category: Biochemistry Authors: Xinbo Deng Ying Zeng Dan Ding Source Type: research

Phytochemical Content of Malus floribunda: In Vitro and Molecular Docking Studies
This study has revealed the idea that this plant, whose phytochemical, antioxidant, and antimicrobial content has been determined, can be used as a bioactive substance equivalent to antibiotics in medicine, the food industry, and human nutrition. In addition, it is expected that the study will contribute to the plant literature. Molecular docking studies were performed to evaluate the binding interactions between the compound and human peroxiredoxin 5 and S. aureus. Both in vitro and in silico results indicated that synthesized extracts could act as potent antioxidant and antimicrobial agents.PMID:38153650 | DOI:10.1007/s1...
Source: Applied Biochemistry and Biotechnology - December 28, 2023 Category: Biochemistry Authors: Ebru Coteli Belgin Erdem Harun Ciftci Source Type: research

Berberine Protects Against Dihydrotestosterone-Induced Human Ovarian Granulosa Cell Injury and Ferroptosis by Regulating the Circ_0097636/MiR-186-5p/SIRT3 Pathway
This study aims to analyze the effect of BBR on a cell model of PCOS and the underlying mechanism. Human ovarian granulosa (KGN) cells were treated with dihydrotestosterone (DHT) to mimic a PCOS cell model. The RNA expression of circ_0097636, miR-186-5p, and sirtuin3 (SIRT3) was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Protein expression was detected by western blotting. Cell viability was analyzed by CCK-8 assay. Cell proliferation and apoptosis were investigated by 5-ethynyl-2'-deoxyuridine (EdU) assay and flow cytometry assay, respectively. The levels of interleukin-6 (IL-6), IL-1β, and...
Source: Applied Biochemistry and Biotechnology - December 28, 2023 Category: Biochemistry Authors: Suqin Wang Yingfang Wang Qin Qin Jianfang Li Qiaoyun Chen Ye Zhang Xiuqing Li Jianrong Liu Source Type: research

Characterization and Validation of a Lyophilized Loop-Mediated Isothermal Amplification Method for the Detection of Esox lucius
This study will be particularly useful for conducting on-site LAMP analyses in the field, where resources for freezing and storage are limited.PMID:38153652 | DOI:10.1007/s12010-023-04799-x (Source: Applied Biochemistry and Biotechnology)
Source: Applied Biochemistry and Biotechnology - December 28, 2023 Category: Biochemistry Authors: Nivedhitha Jothinarayanan Frank Karlsen Lars Eric Roseng Source Type: research

Enhancing Enzyme Stability and Functionality: Covalent Immobilization of Trypsin on Magnetic Gum Arabic Modified Fe 3 O 4 Nanoparticles
This study aimed to fabricate gum Arabic (GA)-coated Fe3O4 nanoparticles bearing numerous active aldehyde groups on their surface, followed by an assessment of their capability as a magnetic support for the covalent immobilization of the trypsin enzyme for the first time. FT-IR, XRD, TGA, and SEM results demonstrated the successful synthesis of GA-coated Fe3O4 nanoparticles, along with the covalent immobilization of the enzyme onto the support. Immobilization enhanced the relative enzymatic activity across a range of aqueous solution pH levels (ranging from 4 to 11) and temperatures (ranging from 20 to 80 °C) without alte...
Source: Applied Biochemistry and Biotechnology - December 28, 2023 Category: Biochemistry Authors: Yasaman Behshad Mohammad Pazhang Saeed Najavand Mohammad Sabzi Source Type: research

Unveiling Xanthine Presence in Rohu Fish Using Ag + -Doped MoS 2 Nanosheets Through Electrochemical Analysis
Appl Biochem Biotechnol. 2023 Dec 28. doi: 10.1007/s12010-023-04823-0. Online ahead of print.ABSTRACTHere, we envisage the development of the rapid, reliable, and facile electrochemical sensor for the primary detection of xanthine (Xn) which is significant for the food quality measurement, based on the silver-doped molybdenum disulfide (Ag@MoS2) nanosheets. The structural and compositional properties of the prepared samples were tested through X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, and X-ray photon spectroscopy (XPS). The two-dimensional (2D) MoS2 nanosheets provide the large surface area...
Source: Applied Biochemistry and Biotechnology - December 28, 2023 Category: Biochemistry Authors: Prateek Sharma Deeksha Thakur Devendra Kumar Omprakash Yadav Source Type: research