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Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research

Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research

Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research

Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research

Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research

Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research

Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research

Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research

Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research

Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research

Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research

Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research

Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research

A Cytotoxicity Assay as an Alternative to the Murine Model for the Potency Testing of < em > Bothrops jararaca < /em > Venom and Antivenom: An Intralaboratory Pre-validation Study
Altern Lab Anim. 2024 Mar 4:2611929241237518. doi: 10.1177/02611929241237518. Online ahead of print.ABSTRACTAntivenom therapy is the only specific treatment for snakebite envenomation, and antivenom potency determination is key in the efficacy assurance quality control process. Nowadays, this process relies on the in vivo murine model - thus, the development of alternative in vitro methods is imperative. In the current study, the principle of the proposed method is the ability of Bothrops venom to induce cytotoxic effects in Vero cells, and the capacity to evaluate the inhibition of this cytotoxicity by the respective anti...
Source: Alternatives to Laboratory Animals : ATLA - March 4, 2024 Category: Research Authors: Renata N C Nundes Antonio E C C Almeida Wlamir C Moura Marcelo S Gonzalez Humberto P Ara újo Source Type: research

A Cytotoxicity Assay as an Alternative to the Murine Model for the Potency Testing of < em > Bothrops jararaca < /em > Venom and Antivenom: An Intralaboratory Pre-validation Study
Altern Lab Anim. 2024 Mar 4:2611929241237518. doi: 10.1177/02611929241237518. Online ahead of print.ABSTRACTAntivenom therapy is the only specific treatment for snakebite envenomation, and antivenom potency determination is key in the efficacy assurance quality control process. Nowadays, this process relies on the in vivo murine model - thus, the development of alternative in vitro methods is imperative. In the current study, the principle of the proposed method is the ability of Bothrops venom to induce cytotoxic effects in Vero cells, and the capacity to evaluate the inhibition of this cytotoxicity by the respective anti...
Source: Alternatives to Laboratory Animals : ATLA - March 4, 2024 Category: Research Authors: Renata N C Nundes Antonio E C C Almeida Wlamir C Moura Marcelo S Gonzalez Humberto P Ara újo Source Type: research