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Conference Diary
Altern Lab Anim. 2024 Apr 9:2611929241245506. doi: 10.1177/02611929241245506. Online ahead of print.NO ABSTRACTPMID:38594898 | DOI:10.1177/02611929241245506 (Source: Alternatives to Laboratory Animals : ATLA)
Source: Alternatives to Laboratory Animals : ATLA - April 10, 2024 Category: Research Source Type: research

Acute Toxicity Assays with the < em > Artemia salina < /em > Model: Assessment of Variables
Altern Lab Anim. 2024 Apr 5:2611929241242443. doi: 10.1177/02611929241242443. Online ahead of print.ABSTRACTThe use of the brine shrimp Artemia salina (Leach) in acute toxicity assays has great potential due to its simplicity, low cost and reproducibility. In the current study, some of the variables that can influence the reliability of the assay in terms of test organism survival, were evaluated as part of its implementation in our laboratory. The quality and type of water used, the buffer components and other parameters (salinity, pH and dissolved oxygen level), were all evaluated for optimisation purposes. DMSO (dimethy...
Source: Alternatives to Laboratory Animals : ATLA - April 5, 2024 Category: Research Authors: Gerson Salay N ádila Lucarelli Tha ís Moura Gascón Samantha Sanches de Carvalho Glaucia Raquel Luciano da Veiga Beatriz da Costa Aguiar Alves Reis Fernando Luiz Affonso Fonseca Source Type: research

Acute Toxicity Assays with the < em > Artemia salina < /em > Model: Assessment of Variables
Altern Lab Anim. 2024 Apr 5:2611929241242443. doi: 10.1177/02611929241242443. Online ahead of print.ABSTRACTThe use of the brine shrimp Artemia salina (Leach) in acute toxicity assays has great potential due to its simplicity, low cost and reproducibility. In the current study, some of the variables that can influence the reliability of the assay in terms of test organism survival, were evaluated as part of its implementation in our laboratory. The quality and type of water used, the buffer components and other parameters (salinity, pH and dissolved oxygen level), were all evaluated for optimisation purposes. DMSO (dimethy...
Source: Alternatives to Laboratory Animals : ATLA - April 5, 2024 Category: Research Authors: Gerson Salay N ádila Lucarelli Tha ís Moura Gascón Samantha Sanches de Carvalho Glaucia Raquel Luciano da Veiga Beatriz da Costa Aguiar Alves Reis Fernando Luiz Affonso Fonseca Source Type: research

Acute Toxicity Assays with the < em > Artemia salina < /em > Model: Assessment of Variables
Altern Lab Anim. 2024 Apr 5:2611929241242443. doi: 10.1177/02611929241242443. Online ahead of print.ABSTRACTThe use of the brine shrimp Artemia salina (Leach) in acute toxicity assays has great potential due to its simplicity, low cost and reproducibility. In the current study, some of the variables that can influence the reliability of the assay in terms of test organism survival, were evaluated as part of its implementation in our laboratory. The quality and type of water used, the buffer components and other parameters (salinity, pH and dissolved oxygen level), were all evaluated for optimisation purposes. DMSO (dimethy...
Source: Alternatives to Laboratory Animals : ATLA - April 5, 2024 Category: Research Authors: Gerson Salay N ádila Lucarelli Tha ís Moura Gascón Samantha Sanches de Carvalho Glaucia Raquel Luciano da Veiga Beatriz da Costa Aguiar Alves Reis Fernando Luiz Affonso Fonseca Source Type: research

Acute Toxicity Assays with the < em > Artemia salina < /em > Model: Assessment of Variables
Altern Lab Anim. 2024 Apr 5:2611929241242443. doi: 10.1177/02611929241242443. Online ahead of print.ABSTRACTThe use of the brine shrimp Artemia salina (Leach) in acute toxicity assays has great potential due to its simplicity, low cost and reproducibility. In the current study, some of the variables that can influence the reliability of the assay in terms of test organism survival, were evaluated as part of its implementation in our laboratory. The quality and type of water used, the buffer components and other parameters (salinity, pH and dissolved oxygen level), were all evaluated for optimisation purposes. DMSO (dimethy...
Source: Alternatives to Laboratory Animals : ATLA - April 5, 2024 Category: Research Authors: Gerson Salay N ádila Lucarelli Tha ís Moura Gascón Samantha Sanches de Carvalho Glaucia Raquel Luciano da Veiga Beatriz da Costa Aguiar Alves Reis Fernando Luiz Affonso Fonseca Source Type: research

Acute Toxicity Assays with the < em > Artemia salina < /em > Model: Assessment of Variables
Altern Lab Anim. 2024 Apr 5:2611929241242443. doi: 10.1177/02611929241242443. Online ahead of print.ABSTRACTThe use of the brine shrimp Artemia salina (Leach) in acute toxicity assays has great potential due to its simplicity, low cost and reproducibility. In the current study, some of the variables that can influence the reliability of the assay in terms of test organism survival, were evaluated as part of its implementation in our laboratory. The quality and type of water used, the buffer components and other parameters (salinity, pH and dissolved oxygen level), were all evaluated for optimisation purposes. DMSO (dimethy...
Source: Alternatives to Laboratory Animals : ATLA - April 5, 2024 Category: Research Authors: Gerson Salay N ádila Lucarelli Tha ís Moura Gascón Samantha Sanches de Carvalho Glaucia Raquel Luciano da Veiga Beatriz da Costa Aguiar Alves Reis Fernando Luiz Affonso Fonseca Source Type: research

Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research

Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research

Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research

Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research

Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research

Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research

Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research

Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research

Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research