Kirigami tripod-based electrode for the development of highly stretchable dengue aptasensor
In this study, the kirigami approach has been utilized to develop a stretchable, movable, and flexible sensor. The constructed stretchable-kirigami electrode helps in adjusting the connection of electrodes without disturbing the electrochemical cell zone during the experiment. To increase the sensitivity of this biosensor we have synthesized Ag-NPs (Silver nanoparticles) via chemical methods and characterized their results with the help of TEM & UV-Vis Spectroscopy. Different electrochemical approaches were used to validate the sensor response i.e., CV (Cyclic voltammetry) and LSV (Linear sweep voltammetry), which exhi...
Source: Biomedical Microdevices - April 1, 2024 Category: Biomedical Engineering Authors: Mohd Rahil Hasan Saumitra Singh Pradakshina Sharma Zaira Azmi Agampreet Singh Dadial Jagriti Narang Source Type: research

Kirigami tripod-based electrode for the development of highly stretchable dengue aptasensor
In this study, the kirigami approach has been utilized to develop a stretchable, movable, and flexible sensor. The constructed stretchable-kirigami electrode helps in adjusting the connection of electrodes without disturbing the electrochemical cell zone during the experiment. To increase the sensitivity of this biosensor we have synthesized Ag-NPs (Silver nanoparticles) via chemical methods and characterized their results with the help of TEM & UV-Vis Spectroscopy. Different electrochemical approaches were used to validate the sensor response i.e., CV (Cyclic voltammetry) and LSV (Linear sweep voltammetry), which exhi...
Source: Biomedical Microdevices - April 1, 2024 Category: Biomedical Engineering Authors: Mohd Rahil Hasan Saumitra Singh Pradakshina Sharma Zaira Azmi Agampreet Singh Dadial Jagriti Narang Source Type: research

Kirigami tripod-based electrode for the development of highly stretchable dengue aptasensor
In this study, the kirigami approach has been utilized to develop a stretchable, movable, and flexible sensor. The constructed stretchable-kirigami electrode helps in adjusting the connection of electrodes without disturbing the electrochemical cell zone during the experiment. To increase the sensitivity of this biosensor we have synthesized Ag-NPs (Silver nanoparticles) via chemical methods and characterized their results with the help of TEM & UV-Vis Spectroscopy. Different electrochemical approaches were used to validate the sensor response i.e., CV (Cyclic voltammetry) and LSV (Linear sweep voltammetry), which exhi...
Source: Biomedical Microdevices - April 1, 2024 Category: Biomedical Engineering Authors: Mohd Rahil Hasan Saumitra Singh Pradakshina Sharma Zaira Azmi Agampreet Singh Dadial Jagriti Narang Source Type: research

Kirigami tripod-based electrode for the development of highly stretchable dengue aptasensor
In this study, the kirigami approach has been utilized to develop a stretchable, movable, and flexible sensor. The constructed stretchable-kirigami electrode helps in adjusting the connection of electrodes without disturbing the electrochemical cell zone during the experiment. To increase the sensitivity of this biosensor we have synthesized Ag-NPs (Silver nanoparticles) via chemical methods and characterized their results with the help of TEM & UV-Vis Spectroscopy. Different electrochemical approaches were used to validate the sensor response i.e., CV (Cyclic voltammetry) and LSV (Linear sweep voltammetry), which exhi...
Source: Biomedical Microdevices - April 1, 2024 Category: Biomedical Engineering Authors: Mohd Rahil Hasan Saumitra Singh Pradakshina Sharma Zaira Azmi Agampreet Singh Dadial Jagriti Narang Source Type: research

Kirigami tripod-based electrode for the development of highly stretchable dengue aptasensor
In this study, the kirigami approach has been utilized to develop a stretchable, movable, and flexible sensor. The constructed stretchable-kirigami electrode helps in adjusting the connection of electrodes without disturbing the electrochemical cell zone during the experiment. To increase the sensitivity of this biosensor we have synthesized Ag-NPs (Silver nanoparticles) via chemical methods and characterized their results with the help of TEM & UV-Vis Spectroscopy. Different electrochemical approaches were used to validate the sensor response i.e., CV (Cyclic voltammetry) and LSV (Linear sweep voltammetry), which exhi...
Source: Biomedical Microdevices - April 1, 2024 Category: Biomedical Engineering Authors: Mohd Rahil Hasan Saumitra Singh Pradakshina Sharma Zaira Azmi Agampreet Singh Dadial Jagriti Narang Source Type: research

Kirigami tripod-based electrode for the development of highly stretchable dengue aptasensor
In this study, the kirigami approach has been utilized to develop a stretchable, movable, and flexible sensor. The constructed stretchable-kirigami electrode helps in adjusting the connection of electrodes without disturbing the electrochemical cell zone during the experiment. To increase the sensitivity of this biosensor we have synthesized Ag-NPs (Silver nanoparticles) via chemical methods and characterized their results with the help of TEM & UV-Vis Spectroscopy. Different electrochemical approaches were used to validate the sensor response i.e., CV (Cyclic voltammetry) and LSV (Linear sweep voltammetry), which exhi...
Source: Biomedical Microdevices - April 1, 2024 Category: Biomedical Engineering Authors: Mohd Rahil Hasan Saumitra Singh Pradakshina Sharma Zaira Azmi Agampreet Singh Dadial Jagriti Narang Source Type: research

Kirigami tripod-based electrode for the development of highly stretchable dengue aptasensor
In this study, the kirigami approach has been utilized to develop a stretchable, movable, and flexible sensor. The constructed stretchable-kirigami electrode helps in adjusting the connection of electrodes without disturbing the electrochemical cell zone during the experiment. To increase the sensitivity of this biosensor we have synthesized Ag-NPs (Silver nanoparticles) via chemical methods and characterized their results with the help of TEM & UV-Vis Spectroscopy. Different electrochemical approaches were used to validate the sensor response i.e., CV (Cyclic voltammetry) and LSV (Linear sweep voltammetry), which exhi...
Source: Biomedical Microdevices - April 1, 2024 Category: Biomedical Engineering Authors: Mohd Rahil Hasan Saumitra Singh Pradakshina Sharma Zaira Azmi Agampreet Singh Dadial Jagriti Narang Source Type: research

Biased virus transmission following sequential coinfection of < i > Aedes aegypti < /i > with dengue and Zika viruses
Conclusions/significanceOur findings suggest that, in contrast to simultaneous arbovirus coinfection in mosquitoes that can promote the transmission and co-circulation of these viruses, sequential coinfection appears to have limited influence on arbovirus transmission dynamics. However, it is important to note that more experimental investigations are needed to refine and expand upon this conclusion. (Source: PLoS Neglected Tropical Diseases)
Source: PLoS Neglected Tropical Diseases - April 1, 2024 Category: Tropical Medicine Authors: Jiameng Peng Source Type: research

Molecular and serological evidence of chikungunya virus infection with high case fatality among pediatric population with acute encephalitis syndrome: first report from Eastern Uttar Pradesh, India
This study advocates constant serological and molecular surveillance of CHIKV in AES endemic regions of India. (Source: European Journal of Clinical Microbiology and Infectious Diseases)
Source: European Journal of Clinical Microbiology and Infectious Diseases - April 1, 2024 Category: Microbiology Source Type: research

Transgenic line for characterizing GABA-receptor expression to study the neural basis of olfaction in the yellow-fever mosquito
The mosquito Aedes aegypti is an important vector of diseases including dengue, Zika, chikungunya, and yellow fever. Olfaction is a critical modality for mosquitoes enabling them to locate hosts, sources of nectar, and sites for oviposition. GABA is an essential neurotransmitter in olfactory processing in the insect brain, including the primary olfactory center, the antennal lobe. Previous work with Ae. aegypti has suggested that antennal lobe inhibition via GABA may be involved in the processing of odors. However, little is known about GABA receptor expression in the mosquito brain, or how they may be involved in odor att...
Source: Frontiers in Physiology - March 28, 2024 Category: Physiology Source Type: research

Development of Multiplex Molecular Assays for Simultaneous Detection of Dengue Serotypes and Chikungunya Virus for Arbovirus Surveillance
Curr Issues Mol Biol. 2024 Mar 6;46(3):2093-2104. doi: 10.3390/cimb46030134.ABSTRACTThe major arboviruses mainly belong to the Bunyaviridae, Togaviridae, and Flaviviridae families, among which the chikungunya virus and dengue virus have emerged as global public health problems. The main objective of this study was to develop specific, sensitive, and cost-effective molecular multiplex RT-PCR and RT-qPCR assays for the rapid and simultaneous detection of CHIKV and the four serotypes of DENV for arbovirus surveillance. Specific primers for all viruses were designed, and one-step multiplex RT-PCR (mRT-PCR) and RT-qPCR (mRT-qPC...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Louis Robert W Belem Sylvester Agha Ibemgbo Michel Kir éopori Gomgnimbou Dileep Kumar Verma Antoinette Kabor é Ankit Kumar Ibrahim Sangar é Sujatha Sunil Source Type: research

Development of Multiplex Molecular Assays for Simultaneous Detection of Dengue Serotypes and Chikungunya Virus for Arbovirus Surveillance
Curr Issues Mol Biol. 2024 Mar 6;46(3):2093-2104. doi: 10.3390/cimb46030134.ABSTRACTThe major arboviruses mainly belong to the Bunyaviridae, Togaviridae, and Flaviviridae families, among which the chikungunya virus and dengue virus have emerged as global public health problems. The main objective of this study was to develop specific, sensitive, and cost-effective molecular multiplex RT-PCR and RT-qPCR assays for the rapid and simultaneous detection of CHIKV and the four serotypes of DENV for arbovirus surveillance. Specific primers for all viruses were designed, and one-step multiplex RT-PCR (mRT-PCR) and RT-qPCR (mRT-qPC...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Louis Robert W Belem Sylvester Agha Ibemgbo Michel Kir éopori Gomgnimbou Dileep Kumar Verma Antoinette Kabor é Ankit Kumar Ibrahim Sangar é Sujatha Sunil Source Type: research

Development of Multiplex Molecular Assays for Simultaneous Detection of Dengue Serotypes and Chikungunya Virus for Arbovirus Surveillance
Curr Issues Mol Biol. 2024 Mar 6;46(3):2093-2104. doi: 10.3390/cimb46030134.ABSTRACTThe major arboviruses mainly belong to the Bunyaviridae, Togaviridae, and Flaviviridae families, among which the chikungunya virus and dengue virus have emerged as global public health problems. The main objective of this study was to develop specific, sensitive, and cost-effective molecular multiplex RT-PCR and RT-qPCR assays for the rapid and simultaneous detection of CHIKV and the four serotypes of DENV for arbovirus surveillance. Specific primers for all viruses were designed, and one-step multiplex RT-PCR (mRT-PCR) and RT-qPCR (mRT-qPC...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Louis Robert W Belem Sylvester Agha Ibemgbo Michel Kir éopori Gomgnimbou Dileep Kumar Verma Antoinette Kabor é Ankit Kumar Ibrahim Sangar é Sujatha Sunil Source Type: research

Development of Multiplex Molecular Assays for Simultaneous Detection of Dengue Serotypes and Chikungunya Virus for Arbovirus Surveillance
Curr Issues Mol Biol. 2024 Mar 6;46(3):2093-2104. doi: 10.3390/cimb46030134.ABSTRACTThe major arboviruses mainly belong to the Bunyaviridae, Togaviridae, and Flaviviridae families, among which the chikungunya virus and dengue virus have emerged as global public health problems. The main objective of this study was to develop specific, sensitive, and cost-effective molecular multiplex RT-PCR and RT-qPCR assays for the rapid and simultaneous detection of CHIKV and the four serotypes of DENV for arbovirus surveillance. Specific primers for all viruses were designed, and one-step multiplex RT-PCR (mRT-PCR) and RT-qPCR (mRT-qPC...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Louis Robert W Belem Sylvester Agha Ibemgbo Michel Kir éopori Gomgnimbou Dileep Kumar Verma Antoinette Kabor é Ankit Kumar Ibrahim Sangar é Sujatha Sunil Source Type: research

Development of Multiplex Molecular Assays for Simultaneous Detection of Dengue Serotypes and Chikungunya Virus for Arbovirus Surveillance
Curr Issues Mol Biol. 2024 Mar 6;46(3):2093-2104. doi: 10.3390/cimb46030134.ABSTRACTThe major arboviruses mainly belong to the Bunyaviridae, Togaviridae, and Flaviviridae families, among which the chikungunya virus and dengue virus have emerged as global public health problems. The main objective of this study was to develop specific, sensitive, and cost-effective molecular multiplex RT-PCR and RT-qPCR assays for the rapid and simultaneous detection of CHIKV and the four serotypes of DENV for arbovirus surveillance. Specific primers for all viruses were designed, and one-step multiplex RT-PCR (mRT-PCR) and RT-qPCR (mRT-qPC...
Source: Current Issues in Molecular Biology - March 27, 2024 Category: Molecular Biology Authors: Louis Robert W Belem Sylvester Agha Ibemgbo Michel Kir éopori Gomgnimbou Dileep Kumar Verma Antoinette Kabor é Ankit Kumar Ibrahim Sangar é Sujatha Sunil Source Type: research