Automated sample preparation for electrospray ionization mass spectrometry based on CLOCK-controlled autonomous centrifugal microfluidics
We report a centrifugal microfluidic device that automatically performs sample preparation under steady-state rotation for clinical applications using mass spectrometry. The autonomous microfluidic device was designed for the control of liquid operation on centrifugal hydrokinetics (CLOCK) paradigm. The reported device was highly stable, with less than 7% variation with respect to the time of each unit operation (sample extraction, mixing, and supernatant extraction) in the preparation process. An agitation mechanism with bubbling was used to mix the sample and organic solvent in this device. We confirmed that the device e...
Source: Biomedical Microdevices - April 9, 2024 Category: Biomedical Engineering Authors: Masahiro Futami Hiroki Naito Satoshi Ninomiya Lee Chuin Chen Tomohiko Iwano Kentaro Yoshimura Yoshiaki Ukita Source Type: research
Automated sample preparation for electrospray ionization mass spectrometry based on CLOCK-controlled autonomous centrifugal microfluidics
We report a centrifugal microfluidic device that automatically performs sample preparation under steady-state rotation for clinical applications using mass spectrometry. The autonomous microfluidic device was designed for the control of liquid operation on centrifugal hydrokinetics (CLOCK) paradigm. The reported device was highly stable, with less than 7% variation with respect to the time of each unit operation (sample extraction, mixing, and supernatant extraction) in the preparation process. An agitation mechanism with bubbling was used to mix the sample and organic solvent in this device. We confirmed that the device e...
Source: Biomedical Microdevices - April 9, 2024 Category: Biomedical Engineering Authors: Masahiro Futami Hiroki Naito Satoshi Ninomiya Lee Chuin Chen Tomohiko Iwano Kentaro Yoshimura Yoshiaki Ukita Source Type: research
Automated sample preparation for electrospray ionization mass spectrometry based on CLOCK-controlled autonomous centrifugal microfluidics
We report a centrifugal microfluidic device that automatically performs sample preparation under steady-state rotation for clinical applications using mass spectrometry. The autonomous microfluidic device was designed for the control of liquid operation on centrifugal hydrokinetics (CLOCK) paradigm. The reported device was highly stable, with less than 7% variation with respect to the time of each unit operation (sample extraction, mixing, and supernatant extraction) in the preparation process. An agitation mechanism with bubbling was used to mix the sample and organic solvent in this device. We confirmed that the device e...
Source: Biomedical Microdevices - April 9, 2024 Category: Biomedical Engineering Authors: Masahiro Futami Hiroki Naito Satoshi Ninomiya Lee Chuin Chen Tomohiko Iwano Kentaro Yoshimura Yoshiaki Ukita Source Type: research
Automated sample preparation for electrospray ionization mass spectrometry based on CLOCK-controlled autonomous centrifugal microfluidics
We report a centrifugal microfluidic device that automatically performs sample preparation under steady-state rotation for clinical applications using mass spectrometry. The autonomous microfluidic device was designed for the control of liquid operation on centrifugal hydrokinetics (CLOCK) paradigm. The reported device was highly stable, with less than 7% variation with respect to the time of each unit operation (sample extraction, mixing, and supernatant extraction) in the preparation process. An agitation mechanism with bubbling was used to mix the sample and organic solvent in this device. We confirmed that the device e...
Source: Biomedical Microdevices - April 9, 2024 Category: Biomedical Engineering Authors: Masahiro Futami Hiroki Naito Satoshi Ninomiya Lee Chuin Chen Tomohiko Iwano Kentaro Yoshimura Yoshiaki Ukita Source Type: research
Automated sample preparation for electrospray ionization mass spectrometry based on CLOCK-controlled autonomous centrifugal microfluidics
We report a centrifugal microfluidic device that automatically performs sample preparation under steady-state rotation for clinical applications using mass spectrometry. The autonomous microfluidic device was designed for the control of liquid operation on centrifugal hydrokinetics (CLOCK) paradigm. The reported device was highly stable, with less than 7% variation with respect to the time of each unit operation (sample extraction, mixing, and supernatant extraction) in the preparation process. An agitation mechanism with bubbling was used to mix the sample and organic solvent in this device. We confirmed that the device e...
Source: Biomedical Microdevices - April 9, 2024 Category: Biomedical Engineering Authors: Masahiro Futami Hiroki Naito Satoshi Ninomiya Lee Chuin Chen Tomohiko Iwano Kentaro Yoshimura Yoshiaki Ukita Source Type: research
Automated sample preparation for electrospray ionization mass spectrometry based on CLOCK-controlled autonomous centrifugal microfluidics
We report a centrifugal microfluidic device that automatically performs sample preparation under steady-state rotation for clinical applications using mass spectrometry. The autonomous microfluidic device was designed for the control of liquid operation on centrifugal hydrokinetics (CLOCK) paradigm. The reported device was highly stable, with less than 7% variation with respect to the time of each unit operation (sample extraction, mixing, and supernatant extraction) in the preparation process. An agitation mechanism with bubbling was used to mix the sample and organic solvent in this device. We confirmed that the device e...
Source: Biomedical Microdevices - April 9, 2024 Category: Biomedical Engineering Authors: Masahiro Futami Hiroki Naito Satoshi Ninomiya Lee Chuin Chen Tomohiko Iwano Kentaro Yoshimura Yoshiaki Ukita Source Type: research
Automated sample preparation for electrospray ionization mass spectrometry based on CLOCK-controlled autonomous centrifugal microfluidics
We report a centrifugal microfluidic device that automatically performs sample preparation under steady-state rotation for clinical applications using mass spectrometry. The autonomous microfluidic device was designed for the control of liquid operation on centrifugal hydrokinetics (CLOCK) paradigm. The reported device was highly stable, with less than 7% variation with respect to the time of each unit operation (sample extraction, mixing, and supernatant extraction) in the preparation process. An agitation mechanism with bubbling was used to mix the sample and organic solvent in this device. We confirmed that the device e...
Source: Biomedical Microdevices - April 9, 2024 Category: Biomedical Engineering Authors: Masahiro Futami Hiroki Naito Satoshi Ninomiya Lee Chuin Chen Tomohiko Iwano Kentaro Yoshimura Yoshiaki Ukita Source Type: research
Kirigami tripod-based electrode for the development of highly stretchable dengue aptasensor
In this study, the kirigami approach has been utilized to develop a stretchable, movable, and flexible sensor. The constructed stretchable-kirigami electrode helps in adjusting the connection of electrodes without disturbing the electrochemical cell zone during the experiment. To increase the sensitivity of this biosensor we have synthesized Ag-NPs (Silver nanoparticles) via chemical methods and characterized their results with the help of TEM & UV-Vis Spectroscopy. Different electrochemical approaches were used to validate the sensor response i.e., CV (Cyclic voltammetry) and LSV (Linear sweep voltammetry), which exhi...
Source: Biomedical Microdevices - April 1, 2024 Category: Biomedical Engineering Authors: Mohd Rahil Hasan Saumitra Singh Pradakshina Sharma Zaira Azmi Agampreet Singh Dadial Jagriti Narang Source Type: research
Kirigami tripod-based electrode for the development of highly stretchable dengue aptasensor
In this study, the kirigami approach has been utilized to develop a stretchable, movable, and flexible sensor. The constructed stretchable-kirigami electrode helps in adjusting the connection of electrodes without disturbing the electrochemical cell zone during the experiment. To increase the sensitivity of this biosensor we have synthesized Ag-NPs (Silver nanoparticles) via chemical methods and characterized their results with the help of TEM & UV-Vis Spectroscopy. Different electrochemical approaches were used to validate the sensor response i.e., CV (Cyclic voltammetry) and LSV (Linear sweep voltammetry), which exhi...
Source: Biomedical Microdevices - April 1, 2024 Category: Biomedical Engineering Authors: Mohd Rahil Hasan Saumitra Singh Pradakshina Sharma Zaira Azmi Agampreet Singh Dadial Jagriti Narang Source Type: research
Kirigami tripod-based electrode for the development of highly stretchable dengue aptasensor
In this study, the kirigami approach has been utilized to develop a stretchable, movable, and flexible sensor. The constructed stretchable-kirigami electrode helps in adjusting the connection of electrodes without disturbing the electrochemical cell zone during the experiment. To increase the sensitivity of this biosensor we have synthesized Ag-NPs (Silver nanoparticles) via chemical methods and characterized their results with the help of TEM & UV-Vis Spectroscopy. Different electrochemical approaches were used to validate the sensor response i.e., CV (Cyclic voltammetry) and LSV (Linear sweep voltammetry), which exhi...
Source: Biomedical Microdevices - April 1, 2024 Category: Biomedical Engineering Authors: Mohd Rahil Hasan Saumitra Singh Pradakshina Sharma Zaira Azmi Agampreet Singh Dadial Jagriti Narang Source Type: research
Kirigami tripod-based electrode for the development of highly stretchable dengue aptasensor
In this study, the kirigami approach has been utilized to develop a stretchable, movable, and flexible sensor. The constructed stretchable-kirigami electrode helps in adjusting the connection of electrodes without disturbing the electrochemical cell zone during the experiment. To increase the sensitivity of this biosensor we have synthesized Ag-NPs (Silver nanoparticles) via chemical methods and characterized their results with the help of TEM & UV-Vis Spectroscopy. Different electrochemical approaches were used to validate the sensor response i.e., CV (Cyclic voltammetry) and LSV (Linear sweep voltammetry), which exhi...
Source: Biomedical Microdevices - April 1, 2024 Category: Biomedical Engineering Authors: Mohd Rahil Hasan Saumitra Singh Pradakshina Sharma Zaira Azmi Agampreet Singh Dadial Jagriti Narang Source Type: research
Kirigami tripod-based electrode for the development of highly stretchable dengue aptasensor
In this study, the kirigami approach has been utilized to develop a stretchable, movable, and flexible sensor. The constructed stretchable-kirigami electrode helps in adjusting the connection of electrodes without disturbing the electrochemical cell zone during the experiment. To increase the sensitivity of this biosensor we have synthesized Ag-NPs (Silver nanoparticles) via chemical methods and characterized their results with the help of TEM & UV-Vis Spectroscopy. Different electrochemical approaches were used to validate the sensor response i.e., CV (Cyclic voltammetry) and LSV (Linear sweep voltammetry), which exhi...
Source: Biomedical Microdevices - April 1, 2024 Category: Biomedical Engineering Authors: Mohd Rahil Hasan Saumitra Singh Pradakshina Sharma Zaira Azmi Agampreet Singh Dadial Jagriti Narang Source Type: research
Kirigami tripod-based electrode for the development of highly stretchable dengue aptasensor
In this study, the kirigami approach has been utilized to develop a stretchable, movable, and flexible sensor. The constructed stretchable-kirigami electrode helps in adjusting the connection of electrodes without disturbing the electrochemical cell zone during the experiment. To increase the sensitivity of this biosensor we have synthesized Ag-NPs (Silver nanoparticles) via chemical methods and characterized their results with the help of TEM & UV-Vis Spectroscopy. Different electrochemical approaches were used to validate the sensor response i.e., CV (Cyclic voltammetry) and LSV (Linear sweep voltammetry), which exhi...
Source: Biomedical Microdevices - April 1, 2024 Category: Biomedical Engineering Authors: Mohd Rahil Hasan Saumitra Singh Pradakshina Sharma Zaira Azmi Agampreet Singh Dadial Jagriti Narang Source Type: research
Kirigami tripod-based electrode for the development of highly stretchable dengue aptasensor
In this study, the kirigami approach has been utilized to develop a stretchable, movable, and flexible sensor. The constructed stretchable-kirigami electrode helps in adjusting the connection of electrodes without disturbing the electrochemical cell zone during the experiment. To increase the sensitivity of this biosensor we have synthesized Ag-NPs (Silver nanoparticles) via chemical methods and characterized their results with the help of TEM & UV-Vis Spectroscopy. Different electrochemical approaches were used to validate the sensor response i.e., CV (Cyclic voltammetry) and LSV (Linear sweep voltammetry), which exhi...
Source: Biomedical Microdevices - April 1, 2024 Category: Biomedical Engineering Authors: Mohd Rahil Hasan Saumitra Singh Pradakshina Sharma Zaira Azmi Agampreet Singh Dadial Jagriti Narang Source Type: research
A rapid and low-cost platform for detection of bacterial based on microchamber PCR microfluidic chip
Biomed Microdevices. 2024 Mar 2;26(2):20. doi: 10.1007/s10544-024-00699-x.ABSTRACTPolymerase chain reaction (PCR) has been considered as the gold standard for detecting nucleic acids. The simple PCR system is of great significance for medical applications in remote areas, especially for the developing countries. Herein, we proposed a low-cost self-assembled platform for microchamber PCR. The working principle is rotating the chamber PCR microfluidic chip between two heaters with fixed temperature to solve the problem of low temperature variation rate. The system consists of two temperature controllers, a screw slide rail, ...
Source: Biomedical Microdevices - March 2, 2024 Category: Biomedical Engineering Authors: Zhenqing Li Xiaolu Ma Zhen Zhang Xiaoyang Wang Bo Yang Jing Yang Yuan Zeng Xujun Yuan Dawei Zhang Yoshinori Yamaguchi Source Type: research
