Maxizyme-mediated suppression of chikungunya virus replication and transmission in transgenic Aedes aegypti mosquitoes
In this report we examine a maxizyme approach to enhance the catalytic activity and prevent virus mutants from escaping these ribozymes. We designed a maxizyme containing minimized (monomer) versions of two hRzs we previously demonstrated to be the most effective in CHIKV suppression. Three versions of CHIKV maxizyme were designed: Active (Mz), inactive (ΔMz), and a connected CHIKV maxizyme (cMz). The maxizymes with their expression units (Ae-tRNA val promoter and its termination signal) were incorporated into lentivirus vectors with selection and visualization markers. Following transformation, selection, and single-cell...
Source: Frontiers in Microbiology - December 22, 2023 Category: Microbiology Source Type: research
Viruses, Vol. 16, Pages 23: A Multiplex Nanopore Sequencing Approach for the Detection of Multiple Arboviral Species
In this study, we integrated nanopore sequencing and the advantages of reverse transcription polymerase chain reaction (RT-PCR) to develop a multiplex RT-PCR protocol for the detection of Chikungunya virus (CHIKV) and several orthoflaviviruses (such as dengue (Orthoflavivirus dengue), Zika (Orthoflavivirus zikaense), yellow fever (Orthoflavivirus flavi), and West Nile (Orthoflavivirus nilense) viruses) in a single reaction, which provides data for sequence-based differentiation of arbovirus lineages. (Source: Viruses)
Source: Viruses - December 22, 2023 Category: Virology Authors: Joilson Xavier Vagner Fonseca Talita Adelino Felipe C. M. Iani Glauco C. Pereira Myrian M. Duarte Mauricio Lima Emerson Castro Carla Oliveira Hegger Fritsch Natalia Guimar ães Ludmila O. Lamounier Fernanda Khouri Barreto Camilo M. M. Braga de Oliveira Cr Tags: Brief Report Source Type: research
TLR agonists as vaccine adjuvants in the prevention of viral infections: an overview
This study focuses on their application in improving vaccine efficacy against key viral infections, including hepatitis B virus (HBV), hepatitis C virus (HCV), human immunodeficiency virus (HIV), SARS-CoV-2, influenza virus, and flaviviruses, including West Nile virus, dengue virus, and chikungunya virus. Vaccines are crucial in preventing microbial infections, including viruses, and adjuvants play a vital role in modulating immune responses. However, there are still many diseases for which effective vaccines are lacking or have limited immune response, posing significant threats to human health. The use of TLR agonists as...
Source: Frontiers in Microbiology - December 21, 2023 Category: Microbiology Source Type: research
Distribuci ón de Aedes albopictus en Ibagué: potencial riesgo de brotes de arbovirosis
CONCLUSIONES: Aedes albopictus está distribuido en todas las comunas de Ibagué, probablemente su dispersión se ha visto favorecida por las condiciones ambientales y sociales de esta región. Se recomienda hacer seguimiento anual a las poblaciones de este vector y realizar una caracterización molecular de los arbovirus encontrados. Además, el conocer la distribución de este mosquito en la ciudad permitirá focalizar las estrategias de control entomológico y prevenir futuros brotes de arbovirosis.PMID:38109139 | DOI:10.7705/biomedica.7010 (Source: Biomedica : Revista del Instituto Nacional de Salud)
Source: Biomedica : Revista del Instituto Nacional de Salud - December 18, 2023 Category: International Medicine & Public Health Authors: Cristian Camilo Canizales Julio Cesar Carranza Gustavo Adolfo Vallejo Daniel Alfonso Urrea Source Type: research
Aedes albopictus distribution in Ibagu é, Colombia: Potential risk of arboviral outbreaks
CONCLUSIONS: Aedes albopictus is distributed throughout all the communes of Ibague. Its dispersion has probably been favored by this region’s environmental and social conditions. We recommend annual monitoring of these vectors populations and molecular characterization of the found arboviruses. Ascertaining this mosquito’s distribution throughout the city will enable focusing entomological control strategies and preventing future arbovirus outbreaks.PMID:38109139 | DOI:10.7705/biomedica.7010 (Source: Biomedica : Revista del Instituto Nacional de Salud)
Source: Biomedica : Revista del Instituto Nacional de Salud - December 18, 2023 Category: International Medicine & Public Health Authors: Cristian Camilo Canizales Julio Cesar Carranza Gustavo Adolfo Vallejo Daniel Alfonso Urrea Source Type: research
