Viruses, Vol. 16, Pages 23: A Multiplex Nanopore Sequencing Approach for the Detection of Multiple Arboviral Species

In this study, we integrated nanopore sequencing and the advantages of reverse transcription polymerase chain reaction (RT-PCR) to develop a multiplex RT-PCR protocol for the detection of Chikungunya virus (CHIKV) and several orthoflaviviruses (such as dengue (Orthoflavivirus dengue), Zika (Orthoflavivirus zikaense), yellow fever (Orthoflavivirus flavi), and West Nile (Orthoflavivirus nilense) viruses) in a single reaction, which provides data for sequence-based differentiation of arbovirus lineages.

Source: Viruses - December 22, 2023 Category: Virology Authors: Joilson Xavier Vagner Fonseca Talita Adelino Felipe C. M. Iani Glauco C. Pereira Myrian M. Duarte Mauricio Lima Emerson Castro Carla Oliveira Hegger Fritsch Natalia Guimar ães Ludmila O. Lamounier Fernanda Khouri Barreto Camilo M. M. Braga de Oliveira Cr Tags: Brief Report Source Type: research