Rhizobium sophorae is the dominant rhizobial symbiont of Vicia faba L. In North China
In this study, rhizobia that nodulated faba bean grown in soils from three sites in North China (Hebei Province) were isolated and characterized. Firstly, isolates were categorized into genotypes by ribosomal IGS PCR-RFLP analysis, then representatives of the different IGS genotypes were further identified by phylogenetic analyses of 16S rRNA, housekeeping (atpD, recA) and nodulation (nodC) gene sequences. Rhizobial distribution based on the IGS genotype was related to the different soil physicochemical features by redundancy analysis. IGS typing and phylogenetic analyses of 16S rRNA and concatenated housekeeping gene sequ...
Source: Systematic and Applied Microbiology - December 30, 2021 Category: Microbiology Authors: Junjie Zhang Shuo Li Nan Wang Tao Yang Brigitte Brunel Mitchell Andrews Xuxiao Zong Entao Wang Source Type: research
Rhizobium sophorae is the dominant rhizobial symbiont of Vicia faba L. In North China
In this study, rhizobia that nodulated faba bean grown in soils from three sites in North China (Hebei Province) were isolated and characterized. Firstly, isolates were categorized into genotypes by ribosomal IGS PCR-RFLP analysis, then representatives of the different IGS genotypes were further identified by phylogenetic analyses of 16S rRNA, housekeeping (atpD, recA) and nodulation (nodC) gene sequences. Rhizobial distribution based on the IGS genotype was related to the different soil physicochemical features by redundancy analysis. IGS typing and phylogenetic analyses of 16S rRNA and concatenated housekeeping gene sequ...
Source: Systematic and Applied Microbiology - December 30, 2021 Category: Microbiology Authors: Junjie Zhang Shuo Li Nan Wang Tao Yang Brigitte Brunel Mitchell Andrews Xuxiao Zong Entao Wang Source Type: research
Rhizobium sophorae is the dominant rhizobial symbiont of Vicia faba L. In North China
In this study, rhizobia that nodulated faba bean grown in soils from three sites in North China (Hebei Province) were isolated and characterized. Firstly, isolates were categorized into genotypes by ribosomal IGS PCR-RFLP analysis, then representatives of the different IGS genotypes were further identified by phylogenetic analyses of 16S rRNA, housekeeping (atpD, recA) and nodulation (nodC) gene sequences. Rhizobial distribution based on the IGS genotype was related to the different soil physicochemical features by redundancy analysis. IGS typing and phylogenetic analyses of 16S rRNA and concatenated housekeeping gene sequ...
Source: Systematic and Applied Microbiology - December 30, 2021 Category: Microbiology Authors: Junjie Zhang Shuo Li Nan Wang Tao Yang Brigitte Brunel Mitchell Andrews Xuxiao Zong Entao Wang Source Type: research
Levodopa-Induced Dyskinesias Related to Vicia faba Ingestion in a Parkinson & #39;s Disease Patient
Alvaro Lambea-Gil, María-Ángeles, Requena-Calleja, Laura Horna-CañeteNeurology India 2021 69(6):1878-1879 (Source: Neurology India)
Source: Neurology India - December 23, 2021 Category: Neurology Authors: Alvaro Lambea-Gil Mar & #237;a- & #193;ngeles Requena-Calleja Laura Horna-Ca & #241;ete Source Type: research
Acute hemolysis and methemoglobinemia secondary to fava beans ingestion in a patient with G6PD deficiency: A case report of a rare co-occurrence
We report a case of a 47-year-old patient who presented with jaundice that started 2 days after eating fava beans.
Diagnoses:
Laboratory investigations revealed anemia with evidence of hemolysis (high reticulocytes count, high indirect bilirubin, bite cells in peripheral smear). Blood gases showed high methemoglobin level. Reduced level of G6PD enzyme confirmed the diagnosis of G6PD deficiency.
Intervention:
The patient was kept on supplemental oxygen. He was counselled to avoid food and drugs that can cause acute hemolysis.
Outcomes:
Oxygen saturation improved gradually. The patient was discharged w...
Source: Medicine - November 24, 2021 Category: Internal Medicine Tags: Research Article: Clinical Case Report Source Type: research
QTL dissection and mining of candidate genes for Ascochyta fabae and Orobanche crenata resistance in faba bean (Vicia faba L.)
Ascochyta blight caused by Ascochyta fabae Speg. and broomrape (Orobanche crenata) are among the economically most significant pathogens of faba bean. Several QTLs conferring resistance against the two pathogens ... (Source: BMC Plant Biology)
Source: BMC Plant Biology - November 22, 2021 Category: Biology Authors: Natalia Gutierrez and Ana M. Torres Tags: Research Source Type: research
Oxidation of pyrazolone pharmaceuticals by peracetic acid: Kinetics, mechanism and genetic toxicity variations
This study evaluated the oxidation of three pyrazolone pharmaceuticals (i.e., Aminopyrine (AMP), Antipyrine (ANT), and Isopropylphenazone (PRP) by PAA. Experimental results showed that PAA exhibited structure selectivity to the above three pharmaceuticals and oxidized AMP with the highest reactivity. The degradation kinetics of AMP was investigated by calculating the apparent second-order rate constants (kapp) under different initial pH. Through kinetic simulation, the second-order rate constants of elementary reactions between AMP (i.e., neutral (AMP0) and protonated (AMP+) species) with PAA (i.e., neutral (PAA0) and anio...
Source: Chemosphere - November 20, 2021 Category: Chemistry Authors: Shunqi Dong Yongze Liu Li Feng Liqiu Zhang Source Type: research
Oxidation of pyrazolone pharmaceuticals by peracetic acid: Kinetics, mechanism and genetic toxicity variations
This study evaluated the oxidation of three pyrazolone pharmaceuticals (i.e., Aminopyrine (AMP), Antipyrine (ANT), and Isopropylphenazone (PRP) by PAA. Experimental results showed that PAA exhibited structure selectivity to the above three pharmaceuticals and oxidized AMP with the highest reactivity. The degradation kinetics of AMP was investigated by calculating the apparent second-order rate constants (kapp) under different initial pH. Through kinetic simulation, the second-order rate constants of elementary reactions between AMP (i.e., neutral (AMP0) and protonated (AMP+) species) with PAA (i.e., neutral (PAA0) and anio...
Source: Chemosphere - November 20, 2021 Category: Chemistry Authors: Shunqi Dong Yongze Liu Li Feng Liqiu Zhang Source Type: research
Oxidation of pyrazolone pharmaceuticals by peracetic acid: Kinetics, mechanism and genetic toxicity variations
This study evaluated the oxidation of three pyrazolone pharmaceuticals (i.e., Aminopyrine (AMP), Antipyrine (ANT), and Isopropylphenazone (PRP) by PAA. Experimental results showed that PAA exhibited structure selectivity to the above three pharmaceuticals and oxidized AMP with the highest reactivity. The degradation kinetics of AMP was investigated by calculating the apparent second-order rate constants (kapp) under different initial pH. Through kinetic simulation, the second-order rate constants of elementary reactions between AMP (i.e., neutral (AMP0) and protonated (AMP+) species) with PAA (i.e., neutral (PAA0) and anio...
Source: Chemosphere - November 20, 2021 Category: Chemistry Authors: Shunqi Dong Yongze Liu Li Feng Liqiu Zhang Source Type: research
Oxidation of pyrazolone pharmaceuticals by peracetic acid: Kinetics, mechanism and genetic toxicity variations
This study evaluated the oxidation of three pyrazolone pharmaceuticals (i.e., Aminopyrine (AMP), Antipyrine (ANT), and Isopropylphenazone (PRP) by PAA. Experimental results showed that PAA exhibited structure selectivity to the above three pharmaceuticals and oxidized AMP with the highest reactivity. The degradation kinetics of AMP was investigated by calculating the apparent second-order rate constants (kapp) under different initial pH. Through kinetic simulation, the second-order rate constants of elementary reactions between AMP (i.e., neutral (AMP0) and protonated (AMP+) species) with PAA (i.e., neutral (PAA0) and anio...
Source: Chemosphere - November 20, 2021 Category: Chemistry Authors: Shunqi Dong Yongze Liu Li Feng Liqiu Zhang Source Type: research
Cyanosis Due to Methemoglobinemia as the Presenting Sign of Glucose-6-Phosphate Dehydrogenase Deficiency in a Child: Diagnostic and Clinical Implications
A previously healthy 3-year-old boy presented with pallor, jaundice, cyanosis, and a 24-hour history of vomiting and anorexia following fava bean ingestion. Clinical examination and laboratory findings were consistent with severe nonimmune hemolytic anemia with methemoglobinemia. Given the patient’s history, a previously unrecognized glucose-6-phosphate dehydrogenase deficiency was suspected and diagnosed. The aim of this article is to delineate the possible coexistence of methemoglobinemia and glucose-6-phosphate dehydrogenase deficiency in children presented with acute hemolysis and discuss its management while reviewi...
Source: Journal of Pediatric Hematology Oncology - October 30, 2021 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research
Hemp and buckwheat are valuable sources of dietary amino acids, beneficially modulating gastrointestinal hormones and promoting satiety in healthy volunteers
ConclusionPlants are valuable sources of amino acids which are promoting satiety. The impact of hemp and buckwheat on GLP-1 and, respectively, BCAAs should be explored further as could be relevant for aid and prevention of chronic diseases such as type 2 diabetes.Study registered with clinicaltrial.gov on 12th July 2013, study ID number: NCT01898351. (Source: European Journal of Nutrition)
Source: European Journal of Nutrition - October 30, 2021 Category: Nutrition Source Type: research
Development of a polyclonal antibodies-based serological methods and a DIG-labelled DNA probe-based molecular method for detection of the Vicia cryptic virus-M in field plants
In this study, a recombinant version of the putative CP of VCV-M (His-CPVCV-M) was produced and used to prepare a polyclonal antiserum against the His-CPVCV-M. Using this antiserum, a Western blot, an immune-dot-blot and an enzyme-linked immunosorbent assay were developed for testing field samples of V. faba for the presence of VCV-M. Additionally, a digoxigenin (DIG)-labelled DNA probe-based Northern blot assay was established for VCV-M genome detection in field samples. The results showed that both the serological and nucleic acid assays could accurately and sensitively detect VCV-M in V. faba. This research represented ...
Source: Journal of Virological Methods - October 14, 2021 Category: Virology Authors: Kun Zhang Xinjian Zhuang Hongmei Xu Haifeng Gan Zhen He Jiahuan Chen Source Type: research
Hallstatt miners consumed blue cheese and beer during the Iron Age and retained a non-Westernized gut microbiome until the Baroque period
Curr Biol. 2021 Oct 8:S0960-9822(21)01271-9. doi: 10.1016/j.cub.2021.09.031. Online ahead of print.ABSTRACTWe subjected human paleofeces dating from the Bronze Age to the Baroque period (18th century AD) to in-depth microscopic, metagenomic, and proteomic analyses. The paleofeces were preserved in the underground salt mines of the UNESCO World Heritage site of Hallstatt in Austria. This allowed us to reconstruct the diet of the former population and gain insights into their ancient gut microbiome composition. Our dietary survey identified bran and glumes of different cereals as some of the most prevalent plant fragments. T...
Source: Current Biology - October 14, 2021 Category: Biology Authors: Frank Maixner Mohamed S Sarhan Kun D Huang Adrian Tett Alexander Schoenafinger Stefania Zingale Aitor Blanco-M íguez Paolo Manghi Jan Cemper-Kiesslich Wilfried Rosendahl Ulrike Kusebauch Seamus R Morrone Michael R Hoopmann Omar Rota-Stabelli Thomas Ratte Source Type: research
