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Development of a polyclonal antibodies-based serological methods and a DIG-labelled DNA probe-based molecular method for detection of the Vicia cryptic virus-M in field plants
In this study, a recombinant version of the putative CP of VCV-M (His-CPVCV-M) was produced and used to prepare a polyclonal antiserum against the His-CPVCV-M. Using this antiserum, a Western blot, an immune-dot-blot and an enzyme-linked immunosorbent assay were developed for testing field samples of V. faba for the presence of VCV-M. Additionally, a digoxigenin (DIG)-labelled DNA probe-based Northern blot assay was established for VCV-M genome detection in field samples. The results showed that both the serological and nucleic acid assays could accurately and sensitively detect VCV-M in V. faba. This research represented ...
Source: Journal of Virological Methods - October 14, 2021 Category: Virology Authors: Kun Zhang Xinjian Zhuang Hongmei Xu Haifeng Gan Zhen He Jiahuan Chen Source Type: research

Hallstatt miners consumed blue cheese and beer during the Iron Age and retained a non-Westernized gut microbiome until the Baroque period
Curr Biol. 2021 Oct 8:S0960-9822(21)01271-9. doi: 10.1016/j.cub.2021.09.031. Online ahead of print.ABSTRACTWe subjected human paleofeces dating from the Bronze Age to the Baroque period (18th century AD) to in-depth microscopic, metagenomic, and proteomic analyses. The paleofeces were preserved in the underground salt mines of the UNESCO World Heritage site of Hallstatt in Austria. This allowed us to reconstruct the diet of the former population and gain insights into their ancient gut microbiome composition. Our dietary survey identified bran and glumes of different cereals as some of the most prevalent plant fragments. T...
Source: Current Biology - October 14, 2021 Category: Biology Authors: Frank Maixner Mohamed S Sarhan Kun D Huang Adrian Tett Alexander Schoenafinger Stefania Zingale Aitor Blanco-M íguez Paolo Manghi Jan Cemper-Kiesslich Wilfried Rosendahl Ulrike Kusebauch Seamus R Morrone Michael R Hoopmann Omar Rota-Stabelli Thomas Ratte Source Type: research

Cannabis sativa L. and Brassica juncea L. grown on arsenic-contaminated industrial soil: potentiality and limitation for phytoremediation
Environ Sci Pollut Res Int. 2021 Oct 12. doi: 10.1007/s11356-021-16673-6. Online ahead of print.ABSTRACTPhytoremediation represents a natural method to remove contaminants from soil. The goal of this study was to investigate the potential of phosphate-assisted phytoremediation by two energy crops, Cannabis sativa L. and Brassica juncea L., for the sustainable remediation of heavily arsenic-contaminated industrial soil. The two species were investigated for uptake, translocation, and physiological effects of arsenic and phosphate in a microcosm test. Although C. sativa and B. juncea were symptomless when grown in arsenic-co...
Source: Environmental Science and Pollution Research International - October 13, 2021 Category: Environmental Health Authors: Carolina Picchi Lucia Giorgetti Elisabetta Morelli Marco Landi Irene Rosellini Martina Grifoni Elisabetta Franchi Gianniantonio Petruzzelli Meri Barbafieri Source Type: research

Retraction notice to "Cytotoxicity and genotoxicity evaluation of polystyrene microplastics on Vicia faba roots" [Volume 288, 1 November 2021, 117821]
Environ Pollut. 2021 Sep 10;288:118109. doi: 10.1016/j.envpol.2021.118109. Online ahead of print.NO ABSTRACTPMID:34523516 | DOI:10.1016/j.envpol.2021.118109 (Source: Environmental Pollution)
Source: Environmental Pollution - September 15, 2021 Category: Environmental Health Authors: Yin Lu Qin Ma Xiaolu Xu Zhefu Yu Tianjiao Guo Yangkai Wu Source Type: research

Electrochemical activation of peroxymonosulfate (PMS) by carbon cloth anode for sulfamethoxazole degradation
Chemosphere. 2021 Aug 28;287(Pt 2):132094. doi: 10.1016/j.chemosphere.2021.132094. Online ahead of print.ABSTRACTElectrochemical activation of peroxymonosulfate (PMS) at carbon cloth anode (E (Carbon cloth Anode)/PMS system) was investigated for sulfamethoxazole (SMX) degradation. The results indicated that PMS could be activated at carbon cloth anode during electrolysis, resulting in the improvement of SMX degradation. The degradation efficiency of SMX was facilitated with the higher PMS concentration and current density, respectively. The degradation rate constant of SMX increased with the rising pH from 3.6 to 6.0, and ...
Source: Chemosphere - September 7, 2021 Category: Chemistry Authors: Jingyi Fu Li Feng Yongze Liu Liqiu Zhang Shuo Li Source Type: research

Morphological, Biochemical and Molecular Characterization of Rhizobia of Faba Bean Plants Grown in North Nile Delta Egypt
Conclusion: The results confirmed that the five Rhizobial strains will be promising as a source of genes for nitrogen fixation and plant growth promotion.PMID:34486343 | DOI:10.3923/pjbs.2021.672.679 (Source: Pakistan Journal of Biological Sciences: PJBS)
Source: Pakistan Journal of Biological Sciences: PJBS - September 6, 2021 Category: Biomedical Science Authors: Doha M Fathy Ahmed S Eldomiaty Hassan I Abd El-Fattah El-Sayed I Mahgoub Abdallah A Hassanin Source Type: research

Bio-Efficacy of Two Algae against Bruchidius incarnatus , Physiological and Cytogenetic Effects on Vicia faba 
Conclusion: Both types of algae are considered a promising Bio-insecticide in controlling stored grain pests and it may be considered Bio-eco-friendly in pest management.PMID:34486337 | DOI:10.3923/pjbs.2021.618.628 (Source: Pakistan Journal of Biological Sciences: PJBS)
Source: Pakistan Journal of Biological Sciences: PJBS - September 6, 2021 Category: Biomedical Science Authors: Rania S Rashwan Maissa M Morsi Source Type: research