Antioxidant and Anti-Inflammatory Activities of Agrimonia pilosa Ledeb. Extract.
Authors: Kim CY, Yu QM, Kong HJ, Lee JY, Yang KM, Seo JS
Abstract
The purpose of this study is to investigate the effect of Agrimonia pilosa Ledeb. extract (APLE) on lipopolysaccharide- (LPS-) induced cell damage in hepatocytes with a focus on antioxidant and anti-inflammatory activities. Total antioxidant and anti-inflammatory activities of APLE itself were analyzed and phytochemical analysis was performed. Moreover, inhibitory effects of APLE on LPS-induced oxidative stress and inflammation were assessed in human HepG2 hepatocytes. APLE was found to exert α,α-diphenyl-β-picrylhydrazyl (DPPH), 2,2'-azi...
Source: Evidence-based Complementary and Alternative Medicine - July 5, 2020 Category: Complementary Medicine Tags: Evid Based Complement Alternat Med Source Type: research
Secondary metabolites isolated from Agrimonia pilosa Ledeb.
Abstract
A pilot study on the ethanol extracts of Agrimonia pilosa found to have anti-α-glucosidase and anti-inflammatory activities. Subsequent chemical study afforded a new phenylethyl isocoumarin glycoside (1) and eight known compounds (2-9). The structure of 1 was elucidated by comprehensive spectroscopic analysis and chemical transformations. All compounds showed modest α-glucosidase inhibitory activity (IC50 values ranging from 36.8 to 210.7 μM), which was lower than that of the positive control acarbose (IC50=301.9 μM). Those compounds except inactive compounds 3 and 6 showed weak anti-infla...
Source: Natural Product Research - June 21, 2020 Category: Biochemistry Authors: Li HR, Li YK, Xiao J, Yang C, Jiang MY, Tian K, Wang W, Li YH, Huang XZ Tags: Nat Prod Res Source Type: research
Molecules, Vol. 25, Pages 2572: Isolation of Isocoumarins and Flavonoids as α-Glucosidase Inhibitors from Agrimonia pilosa L.
Kang
Agrimonia pilosa L. (AP) showed potent α-glucosidase inhibitory (AGI) activity, but it is uncertain what phytochemicals play a key factor. The phytochemical study of AP based on AGI activity led to the isolation of four isocoumarins; agrimonolide (1), agrimonolide-6-O-β-d-glucopyranoside (2), desmethylagrimonolide (3), desmethylagrimonolide-6-O-β-d-glucopyranoside (4), and four flavonoids; luteolin (5), quercetin (6), vitexin (7), and isovitexin (8). The four isocoumarins were isolated as α-glucosidase inhibitors for the first time. Isocoumarins, compound 1 (ag...
Source: Molecules - May 30, 2020 Category: Chemistry Authors: Park Kang Tags: Article Source Type: research
Rapid identification of chemical composition and metabolites of Pingxiao Capsule in vivo using molecular networking and untargeted data-dependent tandem mass spectrometry.
In this study, a ultra-high performance liquid chromatography (UHPLC) system equipped with high resolution Q-Orbitrap mass spectrometry (MS), and comparative global natural product social (GNPS) molecular networking together with the Compound Discoverer (CD) software were used to identify metabolites of PXC in vitro and in vivo. Based on untargeted data-dependent MS2 and data-mining techniques, 89 peaks of alkaloids, flavonoids, organic acid, and phenolic compounds were identified in the PXC 70% methanol extract. Furthermore, 15 absorbed prototype compounds and their metabolites were rapidly confirmed in rat blood. Glucuro...
Source: Biomedical Chromatography : BMC - May 11, 2020 Category: Biomedical Science Authors: Wang D, Fu Z, Xing Y, Tan Y, Han L, Yu H, Wang T Tags: Biomed Chromatogr Source Type: research
Isolation of Chemical Compounds and Essential Oil from Agrimonia asiatica Juz. and Their Antimicrobial and Antiplasmodial Activities.
Authors: Kozykeyeva RA, Datkhayev UM, Srivedavyasasri R, Ajayi TO, Patsayev AK, Kozykeyeva RA, Ross SA
Abstract
Agrimonia asiatica is a perennial plant with deep green color and covered with soft hairs and has a slightly aromatic odor. This genus Agrimonia has been used in traditional medicines of China, Greece, and European countries. It was mainly used as a haemostatic, a tonic for asthenia, and an astringent for diarrhea. Agrimony is part of the division Magnoliophyta; class is represented by order Rosales, family Rosaceae, of the genus Agrimonia. Family Rosaceae-or pink eels-is one of the largest famil...
Source: The Scientific World Journal - April 16, 2020 Category: Science Tags: ScientificWorldJournal Source Type: research
Phytochemical Screening and Detection of Antibacterial Components from Crude Extracts of Some Armenian Herbs Using TLC-Bioautographic Technique.
Abstract
The aim of the research was to explore qualitative and quantitative phytochemical constituents of different extracts from Agrimonia eupatoria L., Hypericum alpestre subsp. polygonifolium (Rupr.) Avet. & Takht., Rumex obtusifolius Willd and Sanguisorba officinalis L. and analyze their bioactive constituents using TLC-bioautography technique. In TLC analysis different solvent systems as mobile phases were used in order to make assumptions about the nature of active antibacterial compounds. The highest total phenolic, flavonoid and tannin content was detected in methanol extract of A. eupatoria (...
Source: Current Microbiology - February 26, 2020 Category: Microbiology Authors: Ginovyan M, Ayvazyan A, Nikoyan A, Tumanyan L, Trchounian A Tags: Curr Microbiol Source Type: research
Protective effect of Agrimonia pilosa polysaccharides on dexamethasone-treated MC3T3-E1 cells via Wnt/ β-Catenin pathway.
Protective effect of Agrimonia pilosa polysaccharides on dexamethasone-treated MC3T3-E1 cells via Wnt/β-Catenin pathway.
J Cell Mol Med. 2020 Jan 19;:
Authors: Huang W, Jin S, Yang W, Tian S, Meng C, Deng H, Wang H
Abstract
A water-soluble polysaccharide (APP-AW) was isolated from Agrimonia pilosa and prepared to three sulphated derivatives (S1, S2 and S3). The results showed that pre-treatment with APP-AW, S1, S2 and S3 each at the concentration of 50 μg/mL for 48 hours was able to prevent cytotoxicity induced by 1 μmol/L dexamethasone (Dex) in MC3T3-E1 cells via inhibition...
Source: J Cell Mol Med - January 18, 2020 Category: Molecular Biology Authors: Huang W, Jin S, Yang W, Tian S, Meng C, Deng H, Wang H Tags: J Cell Mol Med Source Type: research
Agrimonia pilosa polysaccharide and its sulfate derives facilitate cell proliferation and osteogenic differentiation of MC3T3-E1 cells by targeting miR-107.
Abstract
The present study showed that low level of miR-107 in femoral head tissues from rats suffering steroid-induced osteonecrosis of the femoral head (SANFH) was associated with high degree of osteonecrosis and apoptosis. In vitro assay, pretreatment with an Agrimonia pilos polysaccharide (APP-AW) and its sulphated derivatives (S2) at 50 μg/ml for 48 h or overexpressing miR-107 were able to prevent cytotoxicity induced by 1 μM dexamethasone (DEX) in MC3T3-E1 cells via inhibition of apoptosis. Meanwhile, the decreased ALP activity, collagen content, BMP2, Runx2, OSX and OCN protein expression in...
Source: International Journal of Biological Macromolecules - November 27, 2019 Category: Biochemistry Authors: Huang W, Jin S, Yang W, Tian S, Meng C, Deng H, Wang C, Wang H Tags: Int J Biol Macromol Source Type: research
Anti-viral activity of compounds from Agrimonia pilosa and Galla rhois extract mixture.
In conclusion, our results unveiled that anti-HCV activity of Agrimonia pilosa and Galla rhois extract mixture could lead to the development of a novel therapy for HCV infection.
PMID: 31585267 [PubMed - as supplied by publisher] (Source: Bioorganic Chemistry)
Source: Bioorganic Chemistry - September 25, 2019 Category: Chemistry Authors: Kwon JE, Lee YG, Kang JH, Bai YF, Jeong YJ, Baek NI, Seo YJ, Kang SC Tags: Bioorg Chem Source Type: research
Antibacterial effect of traditional food ingredients for healthcare on Helicobacter pylori.
CONCLUSIONS: The study results on antibacterial effect of traditional food ingredients of vegetables and herbs on H. pylori showed that Scutellaria baicalensis, Annona muricata and Agrimonia pilosa can be considered as healthcare functional materials through the inhibition of H. Pylori.
PMID: 31156188 [PubMed - as supplied by publisher] (Source: Technology and Health Care)
Source: Technology and Health Care - May 23, 2019 Category: Medical Devices Authors: Lee JH, Park JS Tags: Technol Health Care Source Type: research
Quasipucciniastrum agrimoniae, gen. et sp. nov. on Agrimonia (Rosaceae) from China
. (Source: Mycology)
Source: Mycology - April 29, 2019 Category: Biology Authors: Xiao-Hua Qi Lei Cai Peng Zhao Source Type: research
A polysaccharide from dried aerial parts of Agrimonia pilosa: Structural Characterization and its potential therapeutic activity for steroid‑induced necrosis of the femoral head (SANFH)
In this study, one homogeneous polysaccharide (APP-AW), with an average molecular weight of 9,550 Da, was purified from Agrimonia pilosa. Analysis by gas chromatography (GC), methylation, UV, Infrared spectra (IR), 1D and 2D nuclear magnetic resonance (NMR) spectroscopy indicated that APP-AW was a β-(1 → 3)-D-glucan. The effect of APP-AW on dexamethasone (Dex)-induced apoptosis in osteoblasts was also examined. Pretreatment of APP-AW (100 μg/ml) significantly attenuated cell loss and apoptosis induced by Dex (1 μM) in osteoblasts as determined by MTT, Annexin V-FITC/ propidium iodide (PI) and Transferase (TdT)-m...
Source: Carbohydrate Polymers - March 6, 2019 Category: Biomedical Science Source Type: research
The polyphenolic-polysaccharide complex of Agrimonia eupatoria L. as an indirect thrombin inhibitor - isolation and chemical characterization.
Abstract
The polyphenolic-polysaccharide complex was isolated from the dried aerial parts of the medicinal plant Agrimonia eupatoria L. using a multi-step process involving the degreasing of the plant material by extraction with organic solvents, followed by extraction with hot alkali, neutralization, further separation with organic solvents and dialysis. The complex was homogeneous with a molecular weight of about 55 × 103 g/mol and consisted mainly of carbohydrates and polyphenols matrix, composed of lignin-related units, with the dominance of dimethoxyphenyl structures. The carbohydrate moiety co...
Source: International Journal of Biological Macromolecules - December 3, 2018 Category: Biochemistry Authors: Tsirigotis-Maniecka M, Pawlaczyk-Graja I, Ziewiecki R, Balicki S, Matulová M, Capek P, Czechowski F, Gancarz R Tags: Int J Biol Macromol Source Type: research
Agrimonia procera exerts antimicrobial effects, modulates the expression of defensins and cytokines in colonocytes and increases the immune response in lipopolysaccharide-challenged piglets
Because antibiotic use in livestock is assumed to contribute to the emerging public health crisis of antibiotic resistance, alternatives are required. Phytogenic additives are extensively studied due to their ... (Source: BMC Veterinary Research)
Source: BMC Veterinary Research - November 15, 2018 Category: Veterinary Research Authors: Tobias Gr äber, Holger Kluge, Sebastian Granica, Gert Horn, Jutta Kalbitz, Corinna Brandsch, Antje Breitenstein, Christine Brütting and Gabriele I. Stangl Tags: Research article Source Type: research
