Rapid identification of chemical composition and metabolites of Pingxiao Capsule in vivo using molecular networking and untargeted data-dependent tandem mass spectrometry.

In this study, a ultra-high performance liquid chromatography (UHPLC) system equipped with high resolution Q-Orbitrap mass spectrometry (MS), and comparative global natural product social (GNPS) molecular networking together with the Compound Discoverer (CD) software were used to identify metabolites of PXC in vitro and in vivo. Based on untargeted data-dependent MS2 and data-mining techniques, 89 peaks of alkaloids, flavonoids, organic acid, and phenolic compounds were identified in the PXC 70% methanol extract. Furthermore, 15 absorbed prototype compounds and their metabolites were rapidly confirmed in rat blood. Glucuronidation, oxidation, methylation, and hydroxylation were the main metabolic pathways. We fully clarified the chemical constituents of PXC and provided a scientific and efficient strategy for rapid discovery and identification of prototypes and their metabolites in rat plasma using HRMS aided by GNPS and CD software. PMID: 32396262 [PubMed - as supplied by publisher]
Source: Biomedical Chromatography : BMC - Category: Biomedical Science Authors: Tags: Biomed Chromatogr Source Type: research