NANOS3 downregulation in Down syndrome hiPSCs during primordial germ cell-like cell differentiation

In this study, we differentiated hiPSCs with trisomy 21 into hPGCLCs. In vitro-derived germ cells from hiPSCs with Down syndrome (DS) express hPGCLC core circuitry,EOMES,SOX17, andPRDM14 at relatively low levels.TFAP2C andPRDM1 were expressed and remained elevated, whereasNANOS3 andNANOG were downregulated in BMP4-induced hiPSCs with DS. The low level ofNANOG andNANOS3 expression might negatively influence hPGCLC generation in DS hiPSCs. We suggest that DS hPGCLCs could be a suitable model for studying human early germ cell development, the epigenetic and molecular mechanisms of PGC specification and formation, as well as related infertility disorders, such as azoospermia and teratozoospermia.
Source: Histochemistry and Cell Biology - Category: Biomedical Science Source Type: research