Tmod-38. development of an in vitro glioblastoma cancer stem cell bank

The Preston A. Wells Jr. Glioma Cell Line Bank began collecting tissue from UF glioma neurosurgical operations in 2014 and has established an organized repository of immortalized glioma cell lines that have been collected and maintained in a manner useful for a wide range of studies. Copies of patient’s consent to donate their tissue is obtained and clinical data from each case is logged into a secured computer database. Tissue is placed in culture as soon as it is received and the line is founded using the serum-free neurosphere method. Once a line is established, it is expanded and 100 vials are cryopreserved and separated into a "working" or "master" cell source. Each cell line is implanted orthotopically into immunocompromised hosts to confirm in vivo tumorgenicity. Expression profiling is used to subtype the line and to provide a database to identify common or unique gene expression characteristics for each patient derived line. Information on the lines, in vitro growth characteristics, stem cell frequency, in vivo tumor formation and the expression profile will be made publically available via UF web servers. The cell lines are hosted at the Florida Center for Brain Tumor Research [FCBTR] and scientists can submit a request form to the Manager to obtain cryopreserved cell lines. This form requires an explanation of the tissue requested (type, number of samples, justification), description of the study, approval and authorization. After the request has been ap...
Source: Neuro-Oncology - Category: Cancer & Oncology Authors: Tags: TUMOR MODELS Source Type: research