What is CRISPR/Cas9?

Introduction Clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 is a gene-editing technology causing a major upheaval in biomedical research. It makes it possible to correct errors in the genome and turn on or off genes in cells and organisms quickly, cheaply and with relative ease. It has a number of laboratory applications including rapid generation of cellular and animal models, functional genomic screens and live imaging of the cellular genome.1 It has already been demonstrated that it can be used to repair defective DNA in mice curing them of genetic disorders,2 and it has been reported that human embryos can be similarly modified.3 Other potential clinical applications include gene therapy, treating infectious diseases such as HIV and engineering autologous patient material to treat cancer and other diseases. In this review we will give an overview of CRISPR/Cas9 with an emphasis on how it...
Source: Archives of Disease in Childhood - Education and Practice - Category: Pediatrics Authors: Tags: Pancreas and biliary tract, Oncology, Open access, Clinical genetics, Molecular genetics, Immunology (including allergy), HIV/AIDS, Muscle disease, Neuromuscular disease, Reproductive medicine, Child health, Cystic fibrosis, Rheumatology, Sexual health Source Type: research