IJERPH, Vol. 19, Pages 14919: Isothiocyanate-Rich Extracts from Cauliflower (Brassica oleracea Var. Botrytis) and Radish (Raphanus sativus) Inhibited Metabolic Activity and Induced ROS in Selected Human HCT116 and HT-29 Colorectal Cancer Cells
IJERPH, Vol. 19, Pages 14919: Isothiocyanate-Rich Extracts from Cauliflower (Brassica oleracea Var. Botrytis) and Radish (Raphanus sativus) Inhibited Metabolic Activity and Induced ROS in Selected Human HCT116 and HT-29 Colorectal Cancer Cells
International Journal of Environmental Research and Public Health doi: 10.3390/ijerph192214919
Authors:
Mardey Liceth Cuellar-Nuñez
Ivan Luzardo-Ocampo
Sarah Lee-Martínez
Michelle Larrauri-Rodríguez
Guadalupe Zaldívar-Lelo de Larrea
Rosa Martha Pérez-Serrano
Nicolás Camacho-Calderón
Cruciferous vegetables such as cauliflower and radish contain isothiocyanates exhibiting chemoprotective effects in vitro and in vivo. This research aimed to assess the impact of cauliflower (CIE) and radish (RIE) isothiocyanate extracts on the metabolic activity, intracellular reactive oxygen species (ROS), and LDH production of selected human colorectal adenocarcinoma cells (HCT116 and HT-29 for early and late colon cancer development, respectively). Non-cancerous colon cells (CCD-33Co) were used as a cytotoxicity control. The CIE samples displayed the highest allyl isothiocyanate (AITC: 12.55 µg/g) contents, whereas RIE was the most abundant in benzyl isothiocyanate (BITC: 15.35 µg/g). Both extracts effectively inhibited HCT116 and HT-29 metabolic activity, but the CIE impact was higher than that of RIE on HCT116 (IC50: 0.56 mg/mL). Assays using the half-inhibitory concentrations (IC50) of all treatme...
Source: International Journal of Environmental Research and Public Health - Category: Environmental Health Authors: Mardey Liceth Cuellar-Nu ñez Ivan Luzardo-Ocampo Sarah Lee-Mart ínez Michelle Larrauri-Rodr íguez Guadalupe Zald ívar-Lelo de Larrea Rosa Martha P érez-Serrano Nicol ás Camacho-Calderón Tags: Article Source Type: research
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