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Total 125 results found since Jan 2013.
RPL39, MLF2 alter tumor initiation and metastasis Cell Biology
We previously described a gene signature for breast cancer stem cells (BCSCs) derived from patient biopsies. Selective shRNA knockdown identified ribosomal protein L39 (RPL39) and myeloid leukemia factor 2 (MLF2) as the top candidates that affect BCSC self-renewal. Knockdown of RPL39 and MLF2 by specific siRNA nanoparticles in patient-derived and...
Source: Proceedings of the National Academy of Sciences - June 17, 2014 Category: Science Authors: Dave, B., Granados-Principal, S., Zhu, R., Benz, S., Rabizadeh, S., Soon-Shiong, P., Yu, K.-D., Shao, Z., Li, X., Gilcrease, M., Lai, Z., Chen, Y., Huang, T. H.- M., Shen, H., Liu, X., Ferrari, M., Zhan, M., Wong, S. T. C., Kumaraswami, M., Mittal, V., Ch Tags: Biological Sciences Source Type: research
Correction for Dong et al., Lipopeptide nanoparticles for potent and selective siRNA delivery in rodents and nonhuman primates Correction
ENGINEERING Correction for “Lipopeptide nanoparticles for potent and selective siRNA delivery in rodents and nonhuman primates,” by Yizhou Dong, Kevin T. Love, J. Robert Dorkin, Sasilada Sirirungruang, Yunlong Zhang, Delai Chen, Roman L. Bogorad, Hao Yin, Yi Chen, Arturo J. Vegas, Christopher A. Alabi, Gaurav Sahay, Karsten T. Olejnik, Weiheng...
Source: Proceedings of the National Academy of Sciences - April 15, 2014 Category: Science Tags: Corrections Source Type: research
Synthetic microRNAs can control infection Microbiology
Systematic genetic perturbation screening in human cells remains technically challenging. Typically, large libraries of chemically synthesized siRNA oligonucleotides are used, each designed to degrade a specific cellular mRNA via the RNA interference (RNAi) mechanism. Here, we report on data from three genome-wide siRNA screens, conducted to uncover host factors required...
Source: Proceedings of the National Academy of Sciences - March 25, 2014 Category: Science Authors: Franceschini, A., Meier, R., Casanova, A., Kreibich, S., Daga, N., Andritschke, D., Dilling, S., Ramo, P., Emmenlauer, M., Kaufmann, A., Conde–Alvarez, R., Low, S. H., Pelkmans, L., Helenius, A., Hardt, W.–D., Dehio, C., von Mering, C. Tags: Biological Sciences Source Type: research
Speeding evolution Engineering
As medicines become increasingly complex, their delivery becomes more challenging. To function, medicines must effectively navigate the body and reach their site of action. When the impediments to their delivery become too great, drug delivery vehicles are used. Small interfering RNA (siRNA) is a complex class of medicine. These duplex...
Source: Proceedings of the National Academy of Sciences - March 18, 2014 Category: Science Authors: Putnam, D. Tags: Commentaries Source Type: research
Lipopeptides and selective siRNA delivery Engineering
siRNA therapeutics have promise for the treatment of a wide range of genetic disorders. Motivated by lipoproteins, we report lipopeptide nanoparticles as potent and selective siRNA carriers with a wide therapeutic index. Lead material cKK-E12 showed potent silencing effects in mice (ED50 ∼ 0.002 mg/kg), rats (ED50
Source: Proceedings of the National Academy of Sciences - March 18, 2014 Category: Science Authors: Dong, Y., Love, K. T., Dorkin, J. R., Sirirungruang, S., Zhang, Y., Chen, D., Bogorad, R. L., Yin, H., Chen, Y., Vegas, A. J., Alabi, C. A., Sahay, G., Olejnik, K. T., Wang, W., Schroeder, A., Lytton-Jean, A. K. R., Siegwart, D. J., Akinc, A., Barnes, C., Tags: Physical Sciences Source Type: research
Editors' Choice
Diversity Down Below | Amplifying Immunity | Counteracting Muscle Aging | Teaching by Retraction | A Nanomechanical Phase Shifter | Safe, Selective, & Specific siRNA | Speedy Side-Kick
Source: Science: Current Issue - February 28, 2014 Category: Science Authors: Andrew M. Sugden Tags: Ecology Source Type: research
Engineering Safe, Selective, & Specific siRNA
RNA interference (RNAi) via small interfering (si) RNAs offers potential as a therapeutic tool for the specific knockdown of disease-associated genes. One limiting factor in the use – [Read More]
Source: Editors' Choice - February 28, 2014 Category: Science Authors: Guy Riddihough Tags: Engineering Source Type: research
Inheritance of Trans Chromosomal Methylation Plant Biology
Hybridization in plants leads to transinteractions between the parental genomes and epigenomes that can result in changes to both 24 nt siRNA and cytosine methylation (mC) levels in the hybrid. In Arabidopsis the principle processes altering the hybrid methylome are Trans Chromosomal Methylation (TCM) and Trans Chromosomal deMethylation (TCdM) in...
Source: Proceedings of the National Academy of Sciences - February 4, 2014 Category: Science Authors: Greaves, I. K., Groszmann, M., Wang, A., Peacock, W. J., Dennis, E. S. Tags: Biological Sciences Source Type: research
Radiation killing is regulated by LGP2 Medical Sciences
An siRNA screen targeting 89 IFN stimulated genes in 14 different cancer cell lines pointed to the RIG-I (retinoic acid inducible gene I)–like receptor Laboratory of Genetics and Physiology 2 (LGP2) as playing a key role in conferring tumor cell survival following cytotoxic stress induced by ionizing radiation (IR). Studies...
Source: Proceedings of the National Academy of Sciences - January 28, 2014 Category: Science Authors: Widau, R. C., Parekh, A. D., Ranck, M. C., Golden, D. W., Kumar, K. A., Sood, R. F., Pitroda, S. P., Liao, Z., Huang, X., Darga, T. E., Xu, D., Huang, L., Andrade, J., Roizman, B., Weichselbaum, R. R., Khodarev, N. N. Tags: PNAS Plus Source Type: research
Consequences of HSV-1-induced AS1 deficiency Microbiology
Herpes simplex virus 1 (HSV-1) infection triggers specific metabolic changes in its host cell. To explore the interactions between cellular metabolism and HSV-1 infection, we performed an siRNA screen of cellular metabolic genes, measuring their effect on viral replication. The screen identified multiple enzymes predicted to influence HSV-1 replication, including...
Source: Proceedings of the National Academy of Sciences - December 17, 2013 Category: Science Authors: Grady, S. L., Purdy, J. G., Rabinowitz, J. D., Shenk, T. Tags: PNAS Plus Source Type: research
Codelivery of siRNA and a cisplatin prodrug Engineering
Cisplatin and other DNA-damaging chemotherapeutics are widely used to treat a broad spectrum of malignancies. However, their application is limited by both intrinsic and acquired chemoresistance. Most mutations that result from DNA damage are the consequence of error-prone translesion DNA synthesis, which could be responsible for the acquired resistance against...
Source: Proceedings of the National Academy of Sciences - November 12, 2013 Category: Science Authors: Xu, X., Xie, K., Zhang, X.-Q., Pridgen, E. M., Park, G. Y., Cui, D. S., Shi, J., Wu, J., Kantoff, P. W., Lippard, S. J., Langer, R., Walker, G. C., Farokhzad, O. C. Tags: Biological Sciences Source Type: research
MAPKAPK2 impairs replication of damaged DNA Cell Biology
DNA damage can obstruct replication forks, resulting in replicative stress. By siRNA screening, we identified kinases involved in the accumulation of phosphohistone 2AX (γH2AX) upon UV irradiation-induced replication stress. Surprisingly, the strongest reduction of phosphohistone 2AX followed knockdown of the MAP kinase-activated protein kinase 2 (MK2), a kinase currently implicated...
Source: Proceedings of the National Academy of Sciences - October 15, 2013 Category: Science Authors: Kopper, F., Bierwirth, C., Schon, M., Kunze, M., Elvers, I., Kranz, D., Saini, P., Menon, M. B., Walter, D., Sorensen, C. S., Gaestel, M., Helleday, T., Schon, M. P., Dobbelstein, M. Tags: Biological Sciences Source Type: research
Evaluation of nanoparticles for siRNA delivery Applied Biological Sciences
Nanoparticle-mediated siRNA delivery is a complex process that requires transport across numerous extracellular and intracellular barriers. As such, the development of nanoparticles for efficient delivery would benefit from an understanding of how parameters associated with these barriers relate to the physicochemical properties of nanoparticles. Here, we use a multiparametric approach...
Source: Proceedings of the National Academy of Sciences - August 6, 2013 Category: Science Authors: Alabi, C. A., Love, K. T., Sahay, G., Yin, H., Luly, K. M., Langer, R., Anderson, D. G. Tags: Physical Sciences Source Type: research
Research Article Regulators of Calcium Homeostasis Identified by Inference of Kinetic Model Parameters from Live Single Cells Perturbed by siRNA
Estimation of parameters in a calcium regulatory network from single-cell data illustrates how this approach can reveal protein function.
Source: Signal Transduction Knowledge Environment - July 10, 2013 Category: Science Authors: Samuel Bandara, Seth Malmersj{ouml}, Tobias Meyer Source Type: news
Down-regulation of eIF5A-2 prevents epithelial-mesenchymal transition in non-small-cell lung cancer cells.
Conclusions: The expression of eIF5A-2 was up-regulated following EMT phenotype changes in A549 cells, which correlated with enhanced tumor invasion and metastatic capabilities. Furthermore, in the A549 cell line, the process of EMT phenotype change could be reversed by eIF5A-2 siRNA, with a consequent weakening of both invasive and metastatic capabilities.
PMID: 23733422 [PubMed - in process]
Source: J Zhejiang Univ Sci ... - June 1, 2013 Category: Science Authors: Xu GD, Shi XB, Sun LB, Zhou QY, Zheng DW, Shi HS, Che YL, Wang ZS, Shao GF Tags: J Zhejiang Univ Sci B Source Type: research
