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Total 125 results found since Jan 2013.
Structurally modulated codelivery of siRNA and Argonaute 2 for enhanced RNA interference Engineering
Small interfering RNA (siRNA) represents a promising class of inhibitors in both fundamental research and the clinic. Numerous delivery vehicles have been developed to facilitate siRNA delivery. Nevertheless, achieving highly potent RNA interference (RNAi) toward clinical translation requires efficient formation of RNA-induced gene-silencing complex (RISC) in the cytoplasm. Here we...
Source: Proceedings of the National Academy of Sciences - March 20, 2018 Category: Science Authors: Jiahe Li, Connie Wu, Wade Wang, Yanpu He, Elad Elkayam, Leemor Joshua-Tor, Paula T. Hammond Tags: PNAS Plus Source Type: research
Genome-wide functional analysis reveals central signaling regulators of lymphatic endothelial cell migration and remodeling
Lymphatic vessels constitute a specialized vasculature that is involved in development, cancer, obesity, and immune regulation. The migration of lymphatic endothelial cells (LECs) is critical for vessel growth (lymphangiogenesis) and vessel remodeling, processes that modify the lymphatic network in response to developmental or pathological demands. Using the publicly accessible results of our genome-wide siRNA screen, we characterized the migratome of primary human LECs and identified individual genes and signaling pathways that regulate LEC migration. We compared our data set with mRNA differential expression data from en...
Source: Signal Transduction Knowledge Environment - October 3, 2017 Category: Science Authors: Williams, S. P., Odell, A. F., Karnezis, T., Farnsworth, R. H., Gould, C. M., Li, J., Paquet-Fifield, S., Harris, N. C., Walter, A., Gregory, J. L., Lamont, S. F., Liu, R., Takano, E. A., Nowell, C. J., Bower, N. I., Resnick, D., Smyth, G. K., Coultas, L. Tags: STKE Research Articles Source Type: news
Dissipative Particle Dynamic Simulation on the Assembly and Release of siRNA/Polymer/Gold Nanoparticles Based Polyplex
This article is protected by copyright. All rights reserved.
Source: AIChE Journal - September 11, 2017 Category: Science Authors: Xiaona Xie, Shouping Xu, Pihui Pi, Jiang Cheng, Xiufang Wen, Xuan Liu, Shengnian Wang Tags: Biomolecular Engineering, Bioengineering, Biochemicals, Biofuels, and Food Source Type: research
Lipophilic siRNA targets albumin in situ and promotes bioavailability, tumor penetration, and carrier-free gene silencing Engineering
Clinical translation of therapies based on small interfering RNA (siRNA) is hampered by siRNA's comprehensively poor pharmacokinetic properties, which necessitate molecule modifications and complex delivery strategies. We sought an alternative approach to commonly used nanoparticle carriers by leveraging the long-lived endogenous serum protein albumin as an siRNA carrier. We synthesized...
Source: Proceedings of the National Academy of Sciences - August 8, 2017 Category: Science Authors: Samantha M. Sarett, Thomas A. Werfel, Linus Lee, Meredith A. Jackson, Kameron V. Kilchrist, Dana Brantley-Sieders, Craig L. Duvall Tags: PNAS Plus Source Type: research
Regulation of claudin ‐4 via p63 in human epithelial cells
P63 is a regulator of cell–cell junction complexes in the epidermis. Claudin‐4 is regulated via various factors in normal epithelial cells and diseases. We found that claudin‐4 was directly regulated via p63 (TAp63 and ΔNp63) in human keratinocytes and nasal epithelial cells. In the epidermis of atopic dermatitis (AD), which contains ΔNp63‐deficient keratinocytes, high expression of claudin‐4 was observed. In primary keratinocytes, downregulation of ΔNp63 by treatment with short interfering RNA (siRNA)‐p63 induced claudin‐4 expression. In nasal epithelial cells in the context of rhinitis or nasal polyps, u...
Source: Annals of the New York Academy of Sciences - July 1, 2017 Category: Science Authors: Takashi Kojima, Takayuki Kohno, Terufumi Kubo, Yakuto Kaneko, Takuya Kakuki, Akito Kakiuchi, Makoto Kurose, Ken ‐ichi Takano, Noriko Ogasawara, Kazufumi Obata, Kazuaki Nomura, Ryo Miyata, Takumi Konno, Shingo Ichimiya, Tetsuo Himi Tags: REVIEW Source Type: research
Correction for Yan et al., Suppression of NF-{kappa}B activity via nanoparticle-based siRNA delivery alters early cartilage responses to inȷury Correction
MEDICAL SCIENCES Correction for “Suppression of NF-κB activity via nanoparticle-based siRNA delivery alters early cartilage responses to injury,” by Huimin Yan, Xin Duan, Hua Pan, Nilsson Holguin, Muhammad Farooq Rai, Antonina Akk, Luke E. Springer, Samuel A. Wickline, Linda J. Sandell, and Christine T. N. Pham, which appeared in issue...
Source: Proceedings of the National Academy of Sciences - May 9, 2017 Category: Science Tags: Corrections Source Type: research
Deubiquitinase YOD1 potentiates YAP/TAZ activities through enhancing ITCH stability Cell Biology
Hippo signaling controls the expression of genes regulating cell proliferation and survival and organ size. The regulation of core components in the Hippo pathway by phosphorylation has been extensively investigated, but the roles of ubiquitination−deubiquitination processes are largely unknown. To identify deubiquitinase(s) that regulates Hippo signaling, we performed unbiased siRNA...
Source: Proceedings of the National Academy of Sciences - May 1, 2017 Category: Science Authors: Youngeun Kim, Wantae Kim, Yonghee Song, Jeong-Rae Kim, Kyungjoo Cho, Hyuk Moon, Simon Weonsang Ro, Eunjeong Seo, Yeon-Mi Ryu, Seung-Jae Myung, Eek-Hoon Jho Tags: Biological Sciences Source Type: research
Molecular therapy set to protect at-risk patients against heart attack and stroke
Even a single dose of a specific ribonucleic acid molecule, known as a small interfering RNA (siRNA), offers patients at high risk of cardiovascular disease long-lasting protection against high LDL cholesterol -- one of the main risk factors for heart attack and stroke -- conclude researchers.
Source: ScienceDaily Headlines - March 30, 2017 Category: Science Source Type: news
Biodegradable and Bioreducible Poly(beta ‐amino ester) Nanoparticles for Intracellular Delivery to Treat Brain Cancer
This article is protected by copyright. All rights reserved.
Source: AIChE Journal - February 28, 2017 Category: Science Authors: Yuan Rui, Gabriella Qui ñones, Jordan J. Green Tags: perspective Source Type: research
Phosphate-binding pocket in Dicer-2 PAZ domain for high-fidelity siRNA production Biochemistry
The enzyme Dicer produces small silencing RNAs such as micro-RNAs (miRNAs) and small interfering RNAs (siRNAs). In Drosophila, Dicer-1 produces ∼22–24-nt miRNAs from pre-miRNAs, whereas Dicer-2 makes 21-nt siRNAs from long double-stranded RNAs (dsRNAs). How Dicer-2 precisely makes 21-nt siRNAs with a remarkably high fidelity is unknown. Here we report...
Source: Proceedings of the National Academy of Sciences - December 5, 2016 Category: Science Authors: Suresh K. Kandasamy, Ryuya Fukunaga Tags: Biological Sciences Source Type: research
FoxO6 affects Plxna4-mediated neuronal migration during mouse cortical development Neuroscience
The forkhead transcription factor FoxO6 is prominently expressed during development of the murine neocortex. However, its function in cortical development is as yet unknown. We now demonstrate that cortical development is altered in FoxO6+/− and FoxO6−/− mice, showing migrating neurons halted in the intermediate zone. Using a FoxO6-directed siRNA approach,...
Source: Proceedings of the National Academy of Sciences - November 7, 2016 Category: Science Authors: Ricardo H. Paap, Saskia Oosterbroek, Cindy M. R. J. Wagemans, Lars von Oerthel, Raymond D. Schellevis, Annemarie J. A. Vastenhouw-van der Linden, Marian J. A. Groot Koerkamp, Marco F. M. Hoekman, Marten P. Smidt Tags: PNAS Plus Source Type: research
FoxO6 is essential in cortical development Neuroscience
The forkhead transcription factor FoxO6 is prominently expressed during development of the murine neocortex. However, its function in cortical development is as yet unknown. We now demonstrate that cortical development is altered in FoxO6+/− and FoxO6−/− mice, showing migrating neurons halted in the intermediate zone. Using a FoxO6-directed siRNA approach,...
Source: Proceedings of the National Academy of Sciences - November 7, 2016 Category: Science Authors: Paap, R. H., Oosterbroek, S., Wagemans, C. M. R. J., von Oerthel, L., Schellevis, R. D., Vastenhouw-van der Linden, A. J. A., Groot Koerkamp, M. J. A., Hoekman, M. F. M., Smidt, M. P. Tags: PNAS Plus Source Type: research
Selective NP delivery to cancer over normal cells Engineering
Conventional chemotherapeutics nonselectively kill all rapidly dividing cells, which produces numerous side effects. To address this challenge, we report the discovery of functional polyesters that are capable of delivering siRNA drugs selectively to lung cancer cells and not to normal lung cells. Selective polyplex nanoparticles (NPs) were identified by high-throughput...
Source: Proceedings of the National Academy of Sciences - September 26, 2016 Category: Science Authors: Yan, Y., Liu, L., Xiong, H., Miller, J. B., Zhou, K., Kos, P., Huffman, K. E., Elkassih, S., Norman, J. W., Carstens, R., Kim, J., Minna, J. D., Siegwart, D. J. Tags: PNAS Plus Source Type: research
Drosophila antiviral response Microbiology
Since its discovery, RNA interference has been identified as involved in many different cellular processes, and as a natural antiviral response in plants, nematodes, and insects. In insects, the small interfering RNA (siRNA) pathway is the major antiviral response. In recent years, the Piwi-interacting RNA (piRNA) pathway also has been...
Source: Proceedings of the National Academy of Sciences - July 18, 2016 Category: Science Authors: Petit, M., Mongelli, V., Frangeul, L., Blanc, H., Jiggins, F., Saleh, M.-C. Tags: PNAS Plus Source Type: research
NIR nanoparticles for imaging and siRNA delivery Medical Sciences
Anaplastic thyroid cancer (ATC), one of the most aggressive solid tumors, is characterized by rapid tumor growth and severe metastasis to other organs. Owing to the lack of effective treatment options, ATC has a mortality rate of ∼100% and median survival of less than 5 months. RNAi nanotechnology represents a...
Source: Proceedings of the National Academy of Sciences - July 11, 2016 Category: Science Authors: Liu, Y., Gunda, V., Zhu, X., Xu, X., Wu, J., Askhatova, D., Farokhzad, O. C., Parangi, S., Shi, J. Tags: Physical Sciences Source Type: research
