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Total 125 results found since Jan 2013.
Inhibition of chemotherapy-related breast tumor EMT by application of redox-sensitive siRNA delivery system CSO-ss-SA/siRNA along with doxorubicin treatment.
Abstract
Metastasis is one of the main reasons causing death in cancer patients. It was reported that chemotherapy might induce metastasis. In order to uncover the mechanism of chemotherapy-induced metastasis and find solutions to inhibit treatment-induced metastasis, the relationship between epithelial-mesenchymal transition (EMT) and doxorubicin (DOX) treatment was investigated and a redox-sensitive small interfering RNA (siRNA) delivery system was designed. DOX-related reactive oxygen species (ROS) were found to be responsible for the invasiveness of tumor cells in vitro, causing enhanced EMT and cytoskeleton r...
Source: J Zhejiang Univ Sci ... - February 29, 2020 Category: Science Authors: Liu X, Zhou XQ, Shang XW, Wang L, Li Y, Yuan H, Hu FQ Tags: J Zhejiang Univ Sci B Source Type: research
The effector mechanism of siRNA spherical nucleic acids Biochemistry
Spherical nucleic acids (SNAs) are nanostructures formed by chemically conjugating short linear strands of oligonucleotides to a nanoparticle template. When made with modified small interfering RNA (siRNA) duplexes, SNAs act as single-entity transfection and gene silencing agents and have been used as lead therapeutic constructs in several disease models. However,...
Source: Proceedings of the National Academy of Sciences - January 20, 2020 Category: Science Authors: Gokay Yamankurt, Robert J. Stawicki, Diana M. Posadas, Joseph Q. Nguyen, Richard W. Carthew, Chad A. Mirkin Tags: Physical Sciences Source Type: research
Comparative Study of the Effects of Ferrochelatase ‐siRNA Transfection Mediated by Ultrasound Microbubbles and Polyethyleneimine in Combination with Low‐dose ALA to Enhance PpIX Accumulation in Human Endometrial Cancer Xenograft Nude Mice Models
Red fluorescence caused by the accumulation of PpIX in endometrial cancer xenografts in nude mice after high ‐dose ALA injection could be detected easily byin vivo animal imaging system, but low ‐dose ALA (1.0 mg kg−1) was injected into xenografts, there was no red fluorescence of tumor. However, knockdown of FECH in nude mice was performed by transfection with FECH ‐siRNA mediated by PEI and ultrasound microbubbles alone or in combination, then 1.0 mg kg−1 ALA was injected, apparent red fluorescence of the xenografts was observed, and the fluorescence intensity was PEI+ ultrasound microbubbles > PEI...
Source: Photochemistry and Photobiology - July 18, 2019 Category: Science Authors: Xian Zhang,
Longfei Chen,
Lvfen Gao,
Xuesong Gao,
Nan Li,
Yuwei Song,
Xinke Huang,
Shaoqiang Lin,
Xiaoyu Wang Tags: Research Article Source Type: research
MAP kinase and autophagy pathways cooperate to maintain RAS mutant cancer cell survival Medical Sciences
Oncogenic mutations in the small GTPase KRAS are frequently found in human cancers, and, currently, there are no effective targeted therapies for these tumors. Using a combinatorial siRNA approach, we analyzed a panel of KRAS mutant colorectal and pancreatic cancer cell lines for their dependency on 28 gene nodes that...
Source: Proceedings of the National Academy of Sciences - March 4, 2019 Category: Science Authors: Chih-Shia Lee, Liam C. Lee, Tina L. Yuan, Sirisha Chakka, Christof Fellmann, Scott W. Lowe, Natasha J. Caplen, Frank McCormick, Ji Luo Tags: PNAS Plus Source Type: research
Comparative Study of the Effects of Ferrochelatase ‐siRNA Transfection Mediated by Ultrasound Microbubbles and Polyethyleneimine in Combination with Low‐dose ALA to Enhance PpIX Accumulation in Human Endometrial Cancer Xenograft Nude Mice Models
Red fluorescence caused by the accumulation of PpIX in endometrial cancer xenografts in nude mice after high ‐dose ALA injection could be detected easily byin vivo animal imaging system, but low ‐dose ALA (1.0 mg kg−1) was injected into xenografts, there was no red fluorescence of tumor. However, knockdown of FECH in nude mice was performed by transfection with FECH ‐siRNA mediated by PEI and ultrasound microbubbles alone or in combination, then 1.0 mg kg−1 ALA was injected, apparent red fluorescence of the xenografts was observed, and the fluorescence intensity was PEI+ ultrasound microbubbles > PEI...
Source: Photochemistry and Photobiology - February 11, 2019 Category: Science Authors: Xian Zhang,
Longfei Chen,
Lvfen Gao,
Xuesong Gao,
Nan Li,
Yuwei Song,
Xinke Huang,
Shaoqiang Lin,
Xiaoyu Wang Tags: Research Article Source Type: research
Dicer functions transcriptionally and posttranscriptionally in a multilayer antiviral defense Microbiology
In antiviral RNA interference (RNAi), Dicer plays a primary role in processing double-stranded RNA (dsRNA) molecules into small-interfering RNAs (siRNAs) that guide Argonaute effectors to posttranscriptional suppression of target viral genes. Here, we show a distinct role for Dicer in the siRNA-independent transcriptional induction of certain host genes upon viral...
Source: Proceedings of the National Academy of Sciences - February 5, 2019 Category: Science Authors: Ida Bagus Andika, Hideki Kondo, Nobuhiro Suzuki Tags: PNAS Plus Source Type: research
The transcription factor SP3 drives TNF-{alpha} expression in response to Smac mimetics
The controlled production and downstream signaling of the inflammatory cytokine tumor necrosis factor–α (TNF-α) are important for immunity and its anticancer effects. Although chronic stimulation with TNF-α is detrimental to the health of the host in several autoimmune and inflammatory disorders, TNF-α—contrary to what its name implies—leads to cancer formation by promoting cell proliferation and survival. Smac mimetic compounds (SMCs), small-molecule antagonists of inhibitor of apoptosis proteins (IAPs), switch the TNF-α signal from promoting survival to promoting death in c...
Source: Signal Transduction Knowledge Environment - January 29, 2019 Category: Science Authors: Beug, S. T., Cheung, H. H., Sanda, T., St-Jean, M., Beauregard, C. E., Mamady, H., Baird, S. D., LaCasse, E. C., Korneluk, R. G. Tags: STKE Research Articles Source Type: news
Comparative Study of the Effects of FECH ‐siRNA Transfection Mediated by Ultrasound Microbubbles and PEI in Combination with Low‐dose ALA to Enhance PpIX Accumulation in Human Endometrial Cancer Xenograft Nude Mice Models
This article is protected by copyright. All rights reserved.
Source: Photochemistry and Photobiology - December 24, 2018 Category: Science Authors: Xian Zhang,
Longfei Chen,
Lvfen Gao,
Xuesong Gao,
Nan Li,
Yuwei Song,
Xinke Huang,
Shaoqiang Lin,
Xiaoyu Wang Tags: Research Article Source Type: research
Integrated proximal proteomics reveals IRS2 as a determinant of cell survival in ALK-driven neuroblastoma
Oncogenic anaplastic lymphoma kinase (ALK) is one of the few druggable targets in neuroblastoma, and therapy resistance to ALK-targeting tyrosine kinase inhibitors (TKIs) comprises an inevitable clinical challenge. Therefore, a better understanding of the oncogenic signaling network rewiring driven by ALK is necessary to improve and guide future therapies. Here, we performed quantitative mass spectrometry–based proteomics on neuroblastoma cells treated with one of three clinically relevant ALK TKIs (crizotinib, LDK378, or lorlatinib) or an experimentally used ALK TKI (TAE684) to unravel aberrant ALK signaling pathway...
Source: Signal Transduction Knowledge Environment - November 20, 2018 Category: Science Authors: Emdal, K. B., Pedersen, A.-K., Bekker-Jensen, D. B., Lundby, A., Claeys, S., De Preter, K., Speleman, F., Francavilla, C., Olsen, J. V. Tags: STKE Research Resources Source Type: news
Erroneous ribosomal RNAs promote the generation of antisense ribosomal siRNA Genetics
Ribosome biogenesis is a multistep process, during which mistakes can occur at any step of pre-rRNA processing, modification, and ribosome assembly. Misprocessed rRNAs are usually detected and degraded by surveillance machineries. Recently, we identified a class of antisense ribosomal siRNAs (risiRNAs) that down-regulate pre-rRNAs through the nuclear RNAi pathway. To...
Source: Proceedings of the National Academy of Sciences - October 2, 2018 Category: Science Authors: Chengming Zhu, Qi Yan, Chenchun Weng, Xinhao Hou, Hui Mao, Dun Liu, Xuezhu Feng, Shouhong Guang Tags: Biological Sciences Source Type: research
Functional coupling of GABAA/B receptors and the channel TRPV4 mediates rapid progesterone signaling in the oviduct
We report a rapid response to P4 and agonists of -aminobutyric acid receptors A and B (GABAA/B) in the mouse oviduct that was characterized by oscillatory Ca2+ signals and increased ciliary beat frequency (CBF). Pharmacological manipulation, genetic ablation, and siRNA-mediated knockdown in oviductal cells, as well as overexpression experiments in HEK 293T cells, confirmed the participation of the cationic channel TRPV4, different subunits of GABAA (α1 to α3, β2, and β3), and GABAB1 in P4-induced responses. TRPV4-mediated Ca2+ entry in close proximity to the inositol trisphosphate receptor was require...
Source: Signal Transduction Knowledge Environment - August 14, 2018 Category: Science Authors: Jung, C., Fernandez-Duenas, V., Plata, C., Garcia-Elias, A., Ciruela, F., Fernandez-Fernandez, J. M., Valverde, M. A. Tags: STKE Research Articles Source Type: news
CDK12-mediated transcriptional regulation of noncanonical NF-{kappa}B components is essential for signaling
Members of the family of nuclear factor B (NF-B) transcription factors are critical for multiple cellular processes, including regulating innate and adaptive immune responses, cell proliferation, and cell survival. Canonical NF-B complexes are retained in the cytoplasm by the inhibitory protein IBα, whereas noncanonical NF-B complexes are retained by p100. Although activation of canonical NF-B signaling through the IBα kinase complex is well studied, few regulators of the NF-B–inducing kinase (NIK)–dependent processing of noncanonical p100 to p52 and the subsequent nuclear translocation of p52 have ...
Source: Signal Transduction Knowledge Environment - July 31, 2018 Category: Science Authors: Henry, K. L., Kellner, D., Bajrami, B., Anderson, J. E., Beyna, M., Bhisetti, G., Cameron, T., Capacci, A. G., Bertolotti-Ciarlet, A., Feng, J., Gao, B., Hopkins, B., Jenkins, T., Li, K., May-Dracka, T., Murugan, P., Wei, R., Zeng, W., Allaire, N., Buckle Tags: STKE Research Articles Source Type: news
Thyroid hormone receptor and ERR{alpha} coordinately regulate mitochondrial fission, mitophagy, biogenesis, and function
Thyroid hormone receptor β1 (THRB1) and estrogen-related receptor α (ESRRA; also known as ERRα) both play important roles in mitochondrial activity. To understand their potential interactions, we performed transcriptome and ChIP-seq analyses and found that many genes that were co-regulated by both THRB1 and ESRRA were involved in mitochondrial metabolic pathways. These included oxidative phosphorylation (OXPHOS), the tricarboxylic acid (TCA) cycle, and β-oxidation of fatty acids. TH increased ESRRA expression and activity in a THRB1-dependent manner through the induction of the transcriptional coactiv...
Source: Signal Transduction Knowledge Environment - June 26, 2018 Category: Science Authors: Singh, B. K., Sinha, R. A., Tripathi, M., Mendoza, A., Ohba, K., Sy, J. A. C., Xie, S. Y., Zhou, J., Ho, J. P., Chang, C.-y., Wu, Y., Giguere, V., Bay, B.-H., Vanacker, J.-M., Ghosh, S., Gauthier, K., Hollenberg, A. N., McDonnell, D. P., Yen, P. M. Tags: STKE Research Articles Source Type: news
Roles of the CSE1L-mediated nuclear import pathway in epigenetic silencing Genetics
Epigenetic silencing can be mediated by various mechanisms, and many regulators remain to be identified. Here, we report a genome-wide siRNA screening to identify regulators essential for maintaining gene repression of a CMV promoter silenced by DNA methylation. We identified CSE1L (chromosome segregation 1 like) as an essential factor for...
Source: Proceedings of the National Academy of Sciences - April 24, 2018 Category: Science Authors: Qiang Dong, Xiang Li, Cheng-Zhi Wang, Shaohua Xu, Gang Yuan, Wei Shao, Baodong Liu, Yong Zheng, Hailin Wang, Xiaoguang Lei, Zhuqiang Zhang, Bing Zhu Tags: PNAS Plus Source Type: research
Toward new design principles for superior gene silencing Engineering
Delivery Is the Key Challenge in siRNA Therapies RNA interference (RNAi) or “gene silencing” is a fascinating mechanism in which a cell utilizes a gene’s own RNA sequence to shut down expression of that gene. This process can be employed to investigate the role of genes in disease and to...
Source: Proceedings of the National Academy of Sciences - March 27, 2018 Category: Science Authors: Rangaramanujam M. Kannan Tags: Commentaries Source Type: research
