Comparative Study of the Effects of Ferrochelatase ‐siRNA Transfection Mediated by Ultrasound Microbubbles and Polyethyleneimine in Combination with Low‐dose ALA to Enhance PpIX Accumulation in Human Endometrial Cancer Xenograft Nude Mice Models

Red fluorescence caused by the accumulation of PpIX in endometrial cancer xenografts in nude mice after high ‐dose ALA injection could be detected easily byin  vivo animal imaging system, but low ‐dose ALA (1.0 mg kg−1) was injected into xenografts, there was no red fluorescence of tumor. However, knockdown of FECH in nude mice was performed by transfection with FECH ‐siRNA mediated by PEI and ultrasound microbubbles alone or in combination, then 1.0 mg kg−1 ALA was injected, apparent red fluorescence of the xenografts was observed, and the fluorescence intensity was PEI+ ultrasound microbubbles  >  PEI >  ultrasound microbubbles. AbstractComparison of the fluorescence intensity caused by the accumulation of PpIX in endometrial cancer xenografts in nude mice after low ‐dose 5‐Aminolevulinic acid (ALA) injection combined with siRNA transfection was mediated by ultrasound microbubbles and polyethyleneimine (PEI) to explore the feasibility of the ultrasound microbubble technique as transfection agents. Knockdown of ferrochelatase (FECH) in human endometrial canc er xenografts in nude mice was performed by transfection with FECH‐siRNA mediated by PEI and ultrasound microbubbles alone or in combination; then, low‐dose ALA was injected. Subsequently, anin  vivo animal imaging system was employed to detect the fluorescence intensity in xenografts. Red fluorescence was observed in xenografts given more than 6.25  mg kg−1 of ALA. When the dose of ...

Source: Photochemistry and Photobiology - July 18, 2019 Category: Science Authors: Xian Zhang, Longfei Chen, Lvfen Gao, Xuesong Gao, Nan Li, Yuwei Song, Xinke Huang, Shaoqiang Lin, Xiaoyu Wang Tags: Research Article Source Type: research