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Total 125 results found since Jan 2013.

Erratum to: Inhibition of chemotherapy-related breast tumor EMT by application of redox-sensitive siRNA delivery system CSO-ss-SA/siRNA along with doxorubicin treatment
J Zhejiang Univ Sci B. 2022 Feb 15;23(2):171-172. doi: 10.1631/jzus.B19e0468.ABSTRACTThe online version of the original article can be found at https://doi.org/10.1631/jzus.B1900468 The original version of this article (Liu et al., 2020) unfortunately contained some mistakes. 1. Figs. 7c and 7d in p.229 were incorrect. The upper left and bottom left pictures in Fig. 7c were accidentally duplicated with the pictures at the same position of Fig. 1a. The upper right and bottom right pictures were mistakenly placed in Fig. 7c. Therefore, the calculation results in Fig. 7d were also mistaken. The correct versions should be as f...
Source: J Zhejiang Univ Sci ... - February 21, 2022 Category: Science Authors: Xuan Liu Xueqing Zhou Xuwei Shang Li Wang Yi Li Hong Yuan Fuqiang Hu Source Type: research

A genome-wide screen uncovers multiple roles for mitochondrial nucleoside diphosphate kinase D in inflammasome activation
Noncanonical inflammasome activation by cytosolic lipopolysaccharide (LPS) is a critical component of the host response to Gram-negative bacteria. Cytosolic LPS recognition in macrophages is preceded by a Toll-like receptor (TLR) priming signal required to induce transcription of inflammasome components and facilitate the metabolic reprograming that fuels the inflammatory response. Using a genome-scale arrayed siRNA screen to find inflammasome regulators in mouse macrophages, we identified the mitochondrial enzyme nucleoside diphosphate kinase D (NDPK-D) as a regulator of both noncanonical and canonical inflammasomes. NDPK...
Source: Signal Transduction Knowledge Environment - August 3, 2021 Category: Science Authors: Ernst, O., Sun, J., Lin, B., Banoth, B., Dorrington, M. G., Liang, J., Schwarz, B., Stromberg, K. A., Katz, S., Vayttaden, S. J., Bradfield, C. J., Slepushkina, N., Rice, C. M., Buehler, E., Khillan, J. S., McVicar, D. W., Bosio, C. M., Bryant, C. E., Sut Tags: STKE Research Articles Source Type: news

Cell-free reconstitution reveals the molecular mechanisms for the initiation of secondary siRNA biogenesis in plants Plant Biology
Secondary small interfering RNA (siRNA) production, triggered by primary small RNA targeting, is critical for proper development and antiviral defense in many organisms. RNA-dependent RNA polymerase (RDR) is a key factor in this pathway. However, how RDR specifically converts the targets of primary small RNAs into double-stranded RNA (dsRNA) intermediates...
Source: Proceedings of the National Academy of Sciences - July 30, 2021 Category: Science Authors: Yuriki Sakurai, Kyungmin Baeg, Andy Y. W. Lam, Keisuke Shoji, Yukihide Tomari, Hiro-oki Iwakawa Tags: Plant Biology Biological Sciences Source Type: research

The effect of RNA polymerase V on 24-nt siRNA accumulation depends on DNA methylation contexts and histone modifications in rice Plant Biology
RNA-directed DNA methylation (RdDM) functions in de novo methylation in CG, CHG, and CHH contexts. Here, we performed map-based cloning of OsNRPE1, which encodes the largest subunit of RNA polymerase V (Pol V), a key regulator of gene silencing and reproductive development in rice. We found that rice Pol V...
Source: Proceedings of the National Academy of Sciences - July 21, 2021 Category: Science Authors: Kezhi Zheng, Lili Wang, Longjun Zeng, Dachao Xu, Zhongxin Guo, Xiquan Gao, Dong-Lei Yang Tags: Plant Biology Biological Sciences Source Type: research

Melanopsin Signaling Pathway in HEK293 Cell Line with Stable Expression of Human Melanopsin: Possible Participation of Phospholipase C beta 4 and Diacylglycerol
AbstractMelanopsin, a member of the G-protein coupled receptors family, is involved in non-image-forming functions including circadian rhythm, sleep regulation, pupil response. In spite of significant research efforts, the signaling cascade involving melanopsin photoactivation remains poorly characterized. Here, we analyzed the effects of photoactivation of melanopsin on phospholipase C (PLC) and diacylglycerol. As anin vitro model, HEK293 cells with stable expression of human melanopsin were used. Although both the PLCβ1 and PLCβ4 subtypes were activated by the cell exposure to blue light, only PLCβ4 appeared to play a...
Source: Photochemistry and Photobiology - May 12, 2021 Category: Science Authors: Olga I. Krzysztynska ‐Kuleta, Magdalena M. Olchawa, Tadeusz J. Sarna Tags: RESEARCH ARTICLE Source Type: research

DNA-inspired nanomaterials for enhanced endosomal escape Engineering
In this study, we developed a family of delivery vehicles called Janus base...
Source: Proceedings of the National Academy of Sciences - May 3, 2021 Category: Science Authors: Jinhyung Lee, Ian Sands, Wuxia Zhang, Libo Zhou, Yupeng Chen Tags: Engineering, Brief Reports Physical Sciences Source Type: research

Broad noncoding transcription suggests genome surveillance by RNA polymerase V Plant Biology
Eukaryotic genomes are pervasively transcribed, yet most transcribed sequences lack conservation or known biological functions. In Arabidopsis thaliana, RNA polymerase V (Pol V) produces noncoding transcripts, which base pair with small interfering RNA (siRNA) and allow specific establishment of RNA-directed DNA methylation (RdDM) on transposable elements. Here, we show that...
Source: Proceedings of the National Academy of Sciences - December 1, 2020 Category: Science Authors: Masayuki Tsuzuki, Shriya Sethuraman, Adriana N. Coke, M. Hafiz Rothi, Alan P. Boyle, Andrzej T. Wierzbicki Tags: Biological Sciences Source Type: research

Anti-inflammatory effects of vinpocetine in LPS-stimulated microglia via activation of AMPK.
Abstract Microglia are the resident immune cells in the central nervous system (CNS), which play important roles in the repair of neuroinflammatory injury. The present study investigated the anti-neuroinflammatory effects of vinpocetine induced by lipopolysaccharide (LPS) in BV2 microglia. BV2 microglia were pretreated with vinpocetine, and then stimulated with LPS (100 ng/mL). The cytotoxicity of BV2 microglia was assessed by MTT assay. The expression levels of nitrite oxide were measured by Griess assay. Proinflammatory cytokines and mediators were determined by Western blot, ELISA, or quantitative real-time PCR...
Source: An Acad Bras Cienc - November 26, 2020 Category: Science Authors: Zhou Q, Guo D, Li X, Wang Y, Ye X, Xue S, Wang X Tags: An Acad Bras Cienc Source Type: research

Viral infection impacts transposable element transcript amounts in Drosophila Genetics
Transposable elements (TEs) are genomic parasites that are found in all genomes, some of which display sequence similarity to certain viruses. In insects, TEs are controlled by the Piwi-interacting small interfering RNA (piRNA) pathway in gonads, while the small interfering RNA (siRNA) pathway is dedicated to TE somatic control and...
Source: Proceedings of the National Academy of Sciences - June 1, 2020 Category: Science Authors: Marlene Roy, Barbara Viginier, Edouard Saint–Michel, Frederick Arnaud, Maxime Ratinier, Marie Fablet Tags: Biological Sciences Source Type: research

Microfluidics enables reliable siRNA drug delivery for inflammatory diseases and tumor targeting
Researchers in the Department of Pharmacy at the Ludwig Maximilian University of Munich are using chips from Dolomite Microfluidics to reliably and consistently produce monodisperse particles for...
Source: The Scientist - May 29, 2020 Category: Science Tags: The Scientist The Marketplace Source Type: news