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Specialty: Genetics & Stem Cells

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Total 1523 results found since Jan 2013.

GSE178987 An RNAi therapeutic targeting hepatic DGAT2 in a genetically obese mouse model of nonalcoholic steatohepatitis
Contributors : Batuhan Yenilmez ; Lawrence Lifshitz ; Michael Czech ; Anastasia KhvorovaSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusNonalcoholic steatohepatitis (NASH) is a severe liver disorder characterized by triglyceride accumulation, severe inflammation, and fibrosis. With the recent increase in prevalence, NASH is now the leading cause of liver transplantation, with no approved therapeutics available. Despite years of research, the exact molecular mechanism of NASH progression is not well understood, but fat accumulation is believed to be the primary driver of the disease. T...
Source: GEO: Gene Expression Omnibus - December 31, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE85496 Microarray mRNA expression profiling in Stat5b knockdown pancreatic beta cell line
Contributors : Malini Laloraya ; Annu JosephSeries Type : Expression profiling by arrayOrganism : Mus musculusStat5b gene is located at IDD4 type 1 diabetes susceptibility interval, and is mutated in NOD mice. This mutation has been linked to diabetes with strong association with autoimmune aetiology. We have treated MIN6 pancreatic beta cell line with Stat5b siRNA and analyzed the differential expression profile in control siRNA treated versus Stat5b siRNA treated cells in an effort to understand the role of Stat5b in diabetes.
Source: GEO: Gene Expression Omnibus - December 31, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by array Mus musculus Source Type: research

GSE86179 Knockdowns of total NFYA and a NFYA long 3 ’ UTR isoform followed by RNA-seq to determine isoform-specific effects on gene expression
Contributors : Thomas J Sweet ; Jeffrey M Bhasin ; Angela H TingSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensAlternative cleavage and polyadenylation (APA) produces multiple isoforms of the Nuclear Transcription Factor Y Subunit Alpha (NFYA) gene with variable length 3? untranslated regions (UTRs). These isoforms have been detected in HCT116 colon cancer cells via polyA-seq and northern blots, as well as colon cancer tissue RNA-seq data. NFYA is a transcription factor that may regulate cell growth and proliferation in cancer and development. To determine if there may be transcripti...
Source: GEO: Gene Expression Omnibus - December 28, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Genes, Vol. 14, Pages 58: DNAJA4 Promotes the Replication of the Chinese Giant Salamander Iridovirus
This study demonstrates the function of the epithelial papilloma of carp (EPC) DNAJA4 in Chinese giant salamander (Andrias davidianus) iridovirus (CGSIV) replication. DNAJA4 consists of 1479 base pairs and encodes a 492 amino acid polypeptide. Sequence analysis has shown that EPC DNAJA4 contains a conserved J domain and shares 84% homology with Danio rerio DNAJA4 and 68% homology with Homo sapiens DNAJA4. EPC DNAJA4 was localized in the cytoplasm, and its expression was significantly upregulated after CGSIV infection. Overexpression of EPC DNAJA4 promotes CGSIV replication and CGSIV DNA replication. siRNA knockdown of DNAJ...
Source: Genes - December 24, 2022 Category: Genetics & Stem Cells Authors: Zijing Liu Daofa Xie Xianhui He Tianhong Zhou Wei Li Tags: Article Source Type: research

Genes, Vol. 13, Pages 2338: Identification and Functional Characterization of CYP4D2 Putatively Associated with & beta;-Cypermethrin Detoxification in Phortica okadai
This study constitutes a foundation to explore further the functions of P. okadai CYP450 genes in insecticide metabolism.
Source: Genes - December 11, 2022 Category: Genetics & Stem Cells Authors: Lingjun Wang Hongri Tang Zhimei Xie Di Li Changzhu Yin Bo Luo Rong Yan Wei Sun Hui Liu Tags: Article Source Type: research

GSE198484 RNA sequencing of HUVECs stimulated with VEGF, OSM+VEGF, or CNTF+CNTFR+VEGF with or without STAT3 knock-down
Contributors : Julian Rapp ; Felicitas BucherSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe role of STAT3 signaling induced by stimulation of IL-6 cytokine family members such as OSM and CNTF on the angiogenic response of endothelial cells is still not fully understood. To assess the impact of this pathway on angiogenesis, this study compares human umbilical vein endothelial cells (HUVECs) with or without STAT3 knock-down introduced by siRNA and stimulation with VEGF, OSM+VEGF and CNTF+VEGF in the presence of the CNTF receptor (CNTFR).
Source: GEO: Gene Expression Omnibus - December 4, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

A Novel Angiogenic Effect of PCSK9- Regulated Genes
CONCLUSION: PCSK9 might play an essential role in angiogenesis, unlike its typical role in lipid metabolism, and MMP9, Caspase3, LEFTY1, NODAL, and EGR1 may be involved in the regulation of angiogenesis as critical genes.PMID:36427678 | DOI:10.1016/j.gene.2022.147051
Source: Gene - November 25, 2022 Category: Genetics & Stem Cells Authors: Xiaopeng Zhan Li Jiang Lufeng Wang Jiwen Liu Sheng Kang Haibo Liu Li Lin Source Type: research

GSE140966 RNA-Seq profiling of circular RNAs and the oncogenic role of circPVT1 in cutaneous squamous cell carcinoma
Contributors : Shuang Chen ; Chundi HeSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensCircular RNAs(circRNAs),which are a type of non-coding RNA with a special structure, have attracted the attention of researchers in recent years.As a high throughput gene screening method, RNA-seq has been widely used in tumor research.In present study,we assessed the expression pattern of circRNAs in CSCC via RNA-seq on CSCC and matched paracancerous samples. We further identified circPVT1 as being overexpressed in CSCC tissues and cells.Subsequently, we identified the characteristics of circPVT1 in...
Source: GEO: Gene Expression Omnibus - November 24, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE191323 Characterization of normal fibroblast transcriptomes upon transient silencing of LTBP2 by RNAseq
Conclusions: Our study explored LTBP2 functions in normal fibroblasts. RNAseq data support its important role. After 48h, LTBP2 silencing induces changes in gene expression of numerous genes involved in fibroblast functions.
Source: GEO: Gene Expression Omnibus - November 22, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

RHDV 3C protein antagonizes type I interferon signaling by cleaving interferon promoter stimulated 1 protein
In this study, we found that during RHDV infection, the expressions of interferon and the interferon-stimulated gene were not activated. We constructed eukaryotic expression plasmids of all RHDV proteins, and found that RHDV 3C protein inhibited poly(I:C)-induced interferon expressions. Using siRNA to interfere with the expressions of TLR3 and MDA5, we found that the MDA5 signal pathway was used by the 3C protein to inhibit poly(I:C)-induced interferon expression. This effect was mediated by cleaving the interferon promoter stimulated 1 (IPS-1) protein. Finally, our study showed that interferon was effective against RHDV i...
Source: Virus Genes - November 21, 2022 Category: Genetics & Stem Cells Source Type: research

GSE214881 Targeting Xkr8 via nanoparticle-mediated in situ codelivery of siRNA and chemotherapy drugs for cancer immunochemotherapy
Contributors : Yuang Chen ; Zhangyi Luo ; Runzi Sun ; Song LiSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusDownregulation of specific proteins named scramblases might enhance tumour immunosuppression. In this paper the authors first show that the scramblase Xrk8 is overexpressed in tumour cells upon treatment with chemotherapeutics, and then developed a nanomedicine platform for co-delivery of a cancer pro-drug and of a siRNA directed against the Xrk8 gene, showing therapeutic effect and enhanced immune response in animal tumour models.
Source: GEO: Gene Expression Omnibus - November 18, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE197308 siRNAs targeting a chromatin-associated RNA induce its transcriptional silencing in human cells
Contributors : Julien Ouvrard ; Marion Aguirrebengoa ; Estelle Nicolas ; Didier TroucheSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTranscriptional gene silencing by small interfering RNAs (siRNAs) has been widely described in various species, such as plants or fission yeasts. In mammals, its extent remained somewhat debated. Previous studies showed that siRNAs targeting gene promoters can induce the silencing of the targeted promoter, although the involvement of off-target mechanisms was also suggested. Here, by nascent RNA capture and RNA Pol II ChIP, we show that siRNAs targetin...
Source: GEO: Gene Expression Omnibus - November 18, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Genes, Vol. 13, Pages 2147: Anticipating the Next Chess Move: Blocking SARS-CoV-2 Replication and Simultaneously Disarming Viral Escape Mechanisms
Assumpção The COVID-19 pandemic initiated a race to determine the best measures to control the disease and to save as many people as possible. Efforts to implement social distancing, the use of masks, and massive vaccination programs turned out to be essential in reducing the devastating effects of the pandemic. Nevertheless, the high mutation rates of SARS-CoV-2 challenge the vaccination strategy and maintain the threat of new outbreaks due to the risk of infection surges and even lethal variations able to resist the effects of vaccines and upset the balance. Most of the new therapies tested against SARS-CoV-2 came ...
Source: Genes - November 18, 2022 Category: Genetics & Stem Cells Authors: Samir Mansour Moraes Casseb Andr é Salim Khayat Jorge Estefano Santana de Souza Edivaldo Herculano Correa de Oliveira Sidney Emanuel Batista Dos Santos Pedro Fernando da Costa Vasconcelos Paulo Pimentel de Assump ção Tags: Article Source Type: research

GSE199526 Early growth response 1 transcription factor is essential for the pathogenic properties of human endometriotic epithelial cells
Contributors : Vineet K Maurya ; Maria M Szwarc ; Rodrigo Fernandez-Valdivia ; David M Lonard ; Yong Song ; Niraj Joshi ; Asgerally T Fazleabas ; John P LydonSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensThe aim of this study is to compare transcriptome profiling of EGR1 siRNA treated immortalized human endometriotic epithelial cells expression luciferase (iHEECs/Luc) by RNA-sequencingMethods: The mRNA profiles of non-targeting (NT) and EGR1 siRNA treated immortalized human endometriotic epithelial cells expression luciferase (iHEECs/Luc) were generated by deep sequencing, in tripli...
Source: GEO: Gene Expression Omnibus - November 15, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research