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Specialty: Genetics & Stem Cells

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Total 1523 results found since Jan 2013.

GSE87497 Evaluating Side Effects of Nanoparticle-mediated siRNA Delivery to Mesenchymal Stem Cells Using Next-generation Sequencing and Enrichment Analysis
This study investigates both intended and unintended effects of diblock copolymer nanoparticle (NP) delivery of siRNA delivery to human mesenchymal stem cells (hMSC). Specifically, siRNA delivery was investigated at a range of NP-siRNA:hMSC ratios with a focus on the effects of NP-siRNA treatment on hMSC functions. Additionally, next-generation RNA sequencing (RNA-seq) was used with enrichment analysis to observe side effects in hMSC gene expression. Results show NP-siRNA delivery is negatively correlated with hMSC density. However, higher NP-siRNA:hMSC ratios increased cytotoxicity and decreased metabolic activity. hMSC p...
Source: GEO: Gene Expression Omnibus - September 30, 2016 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

In vivo efficacy and off-target effects of locked nucleic acid (LNA) and unlocked nucleic acid (UNA) modified siRNA and small internally segmented interfering RNA (sisiRNA) in mice bearing human tumor xenografts.
We report that LNA and UNA modified siRNA and sisiRNA improve the efficacy in target knockdown as compared with unmodified siRNA in the tumor xenografts without formulation. However, the level of off-target gene regulation in both the tumor and the liver correlated with the increase in efficacy in target knockdown, unless the seed region of the siRNA was modified. PMID: 21687525 [PubMed - as supplied by publisher]
Source: Artificial DNA: PNA and XNA - November 19, 2015 Category: Genetics & Stem Cells Tags: Artif DNA PNA XNA Source Type: research

A novel siRNA validation system for functional screening of effective RNAi targets in mammalian cells and development of a derivative lentivirus delivery system.
Abstract RNA interference technology is a widely used tool for the regulation of gene expression at the post-transcriptional level. One major challenge is to find the effective short interfering (si)RNA for target gene rapidly and easily, and then to deliver the siRNA into cells or tissues with high efficiency. Here, we designed a novel siRNA validation vector using a dual luciferase reporter system for the functional screening of effective RNAi targets in mammalian cells. Then, based on a siRNA expression cassette, we developed a derivative lentivirus delivery system to infect the appropriate cells or tissues for...
Source: Gene - December 27, 2014 Category: Genetics & Stem Cells Authors: Huang G, Gao Q, Zhao Y, Dong Z, Li T, Guan X, Jiang J Tags: Gene Source Type: research

siRNA potency enhancement via chemical modifications of nucleotide bases at the 5'-end of the siRNA guide strand.
Abstract Small interfering RNAs (siRNAs) can be utilized not only as functional biological research tools but also as therapeutic agents. For the clinical use of siRNA as drugs, various chemical modifications have been employed to improve the activity of siRNA drugs, and further chemical modifications are expected to improve the utility of siRNA therapeutics. As the 5' nucleobase of the guide strand affects the interaction between an siRNA and AGO2 and target cleavage activity, structural optimization of this specific position may be a useful strategy for improving siRNA activity. Here, using the in silico model o...
Source: RNA - November 11, 2020 Category: Genetics & Stem Cells Authors: Shinohara F, Oashi T, Harumoto T, Nishikawa T, Takayama Y, Miyagi H, Takahashi Y, Nakajima T, Sawada T, Koda Y, Makino A, Sato A, Hamaguch K, Suzuki M, Yamamoto J, Tomari Y, Saito JI Tags: RNA Source Type: research

Bifunctional siRNA containing immunostimulatory motif 2 enhances protection against pandemic H1N1 virus infection.
CONCLUSIONS: This study paves the way for broad-spectrum RNAi-based therapeutics using immunostimulatory motif towards improved antiviral effect. Hence this approach will be useful to confront the sudden emergence of pandemic strains. PMID: 26264705 [PubMed - as supplied by publisher]
Source: Current Gene Therapy - August 12, 2015 Category: Genetics & Stem Cells Authors: Joshi G, Dash PK, Agarwal A, Sharma S, Parida M Tags: Curr Gene Ther Source Type: research

Effects of particle size and binding affinity for siRNA on the cellular processing, intestinal permeation, and anti‐inflammatory efficacy of polymeric nanoparticles
ConclusionNanoparticles with particle size of 450 nm or more balanced binding affinity for siRNA might be preferable for the treatment of ulcerative colitis. This article is protected by copyright. All rights reserved.
Source: The Journal of Gene Medicine - August 9, 2015 Category: Genetics & Stem Cells Authors: Wenyue Cheng, Cui Tang, Chunhua Yin Tags: Research Article Source Type: research

Designing an effective therapeutic siRNA to silence RdRp gene of SARS-CoV-2
Infect Genet Evol. 2021 Jun 2:104951. doi: 10.1016/j.meegid.2021.104951. Online ahead of print.ABSTRACTThe devastating outbreak of COVID-19 has spread all over the world and has become a global health concern. There is no specific therapeutics to encounter the COVID-19. Small interfering RNA (siRNA)-based therapy is an efficient strategy to control human viral infections employing post-transcriptional gene silencing (PTGS) through neutralizing target complementary mRNA. RNA-dependent RNA polymerase (RdRp) encoded by the viral RdRp gene as a part of the replication-transcription complex can be adopted as an acceptable targe...
Source: Infection, Genetics and Evolution - June 5, 2021 Category: Genetics & Stem Cells Authors: Mohammad Mahfuz Ali Khan Shawan Ashish Ranjan Sharma Manojit Bhattacharya Bidyut Mallik Farhana Akhter Md Salman Shakil Md Mozammel Hossain Subrata Banik Sang-Soo Lee Md Ashraful Hasan Chiranjib Chakraborty Source Type: research

Genes, Vol. 13, Pages 319: The siRNA Off-Target Effect Is Determined by Base-Pairing Stabilities of Two Different Regions with Opposite Effects
In this study, we investigated the most responsible region for the off-target effect by conducting a comprehensive analysis of the thermodynamic properties of all possible siRNA subregions that involved a machine learning technique using a random sampling procedure. As a result, the thermodynamic stability of nucleotides 2–5 showed the highest positive correlation with the off-target effect, and nucleotides 8–14 showed the most negative correlation. Thus, it is revealed that the siRNA off-target effect is determined by the base-pairing stabilities of two different subregions with opposite effects.
Source: Genes - February 9, 2022 Category: Genetics & Stem Cells Authors: Yoshiaki Kobayashi Shen Tian Kumiko Ui-Tei Tags: Article Source Type: research

Exosomes-delivered PD-L1 siRNA and CTLA-4 siRNA protect against growth and tumor immune escape in colorectal cancer
CONCLUSION: Exosomes containing PD-L1 siRNA and CTLA-4 siRNA suppressed CRC progression and enhanced tumor immune responses.PMID:37217085 | DOI:10.1016/j.ygeno.2023.110646
Source: Genomics - May 22, 2023 Category: Genetics & Stem Cells Authors: Jian Li Yuxiang Chen Mingmei Liao Shuyi Yu Binwen Yuan Zeming Jia Lin Zhou Yaping Tang Source Type: research

Soft Computing Model for Optimized siRNA Design by identifying Off Target possibilities using Artificial Neural Network Model.
Abstract The ability of small interfering RNA (siRNA) to do post transcriptional gene regulation by knocking down targeted genes is an important research topic in functional genomics, biomedical research and in cancer therapeutics. Many tools had been developed to design exogenous siRNA with high experimental inhibition. Even though considerable amount of work has been done in designing exogenous siRNA, design of effective siRNA sequences is still a challenging work because the target mRNAs must be selected such that their corresponding siRNAs are likely to be efficient against that target and unlikely to accident...
Source: Gene - February 25, 2015 Category: Genetics & Stem Cells Authors: Murali R, John PG, S DP Tags: Gene Source Type: research

Co ‐delivery of HIF‐1α siRNA and 5‐fluorouracil to overcome drug resistance in gastric cancer SGC‐7901 cells
ConclusionsCo‐delivery of siRNA and 5‐fluorouracil chitosan nanoparticles is an attractive strategy to overcome multidrug resistance.
Source: The Journal of Gene Medicine - September 1, 2017 Category: Genetics & Stem Cells Authors: Yunna Chen, Li Sun, Dongdong Guo, Ziteng Wu, Weidong Chen Tags: RESEARCH ARTICLE Source Type: research

Genes, Vol. 12, Pages 1102: Silencing of HMGA2 by siRNA Loaded Methotrexate Functionalized Polyamidoamine Dendrimer for Human Breast Cancer Cell Therapy
ati Nazhad Dolatabadi The transcription factor high mobility group protein A2 (HMGA2) plays an important role in the pathogenesis of some cancers including breast cancer. Polyamidoamine dendrimer generation 4 is a kind of highly branched polymeric nanoparticle with surface charge and highest density peripheral groups that allow ligands or therapeutic agents to attach it, thereby facilitating target delivery. Here, methotrexate (MTX)- modified polyamidoamine dendrimer generation 4 (G4) (G4/MTX) was generated to deliver specific small interface RNA (siRNA) for suppressing HMGA2 expression and the consequent effects on fo...
Source: Genes - July 20, 2021 Category: Genetics & Stem Cells Authors: Fereydoon Abedi Gaballu William Chi-Shing Cho Gholamreza Dehghan Amir Zarebkohan Behzad Baradaran Behzad Mansoori Soheil Abbaspour-Ravasjani Ali Mohammadi Nader Sheibani Ayuob Aghanejad Jafar Ezzati Nazhad Dolatabadi Tags: Article Source Type: research

GSE194248 Human prostate cancer cells (VCaP and AIVCaP): siAR alone (single siRNA) vs. siAR plus OPRK1 (combo-siRNA)
Contributors : Yuki Makino ; Takashi KobayashiSeries Type : Expression profiling by arrayOrganism : Homo sapiensThrough comparative genomics using PDX models of androgen-dependent (AD) and castration-resistant (CR) tumors, we identify opioid receptor kappa 1 (OPRK1) as being associated with castration-resistance. Loss of OPRK1 function delays castration-resistance and inhibits castration-resistant growth of prostate cancer cells in culture and in vivo, suggesting OPRK1 as a therapeutic target.To gain insight with regard to biological function of OPRK1 under androgen-depleted condition, we set up two distinct comparisons us...
Source: GEO: Gene Expression Omnibus - April 3, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research