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Specialty: Genetics & Stem Cells

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Total 1523 results found since Jan 2013.

GSE164973 Functional role of HSPA5 in trophoblasts
Contributors : Antti Haapalainen ; Heli Tiensuu ; Pinja TissarinenSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensAnalysis of the effect of HSPA5 siRNA experiment
Source: GEO: Gene Expression Omnibus - February 7, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE208415 TXNIP loss expands Myc transcriptome RNA-seq
Contributors : Tian-Yeh Lim ; Blake R Wilde ; Donald E AyerSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTo better understand how TXNIP in the contributes to the poor prognosis of triple-negative breast cancers, our lab has generated TXNIP null MDA-MB-231 cells (231:TKO). We characterized the gene exrpession programs of these cells by RNA-seq. To determine the Myc-dependence of the transcriptional programs of 231:TKO cells, we used an siRNA RNA approach to reduce Myc levels.
Source: GEO: Gene Expression Omnibus - February 7, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE196260 A global transcriptome analysis of human dermal fibroblasts upon knockdown of the circular RNAs circGLIS3 (circBase ID: hsa_circ_0002874)
Contributors : Ning X Land én ; Maria A TomaSeries Type : Expression profiling by arrayOrganism : Homo sapiensIn an RNAseq analysis, we have identified circGLIS3 with high levels acute wound dermis compared to skinThe biological function of circGLIS3 in human dermal fibroblasts during wound repair has not been studied.To study the genes regulated by circGLIS3, we transfected siRNA targeting the diagnostic junctions of circGLIS3 into human dermal fibroblasts to knockdown circGLIS3 expression.We performed a global transcriptome analysis of keratinocytes upon circRNA knockdown using Affymetrix arrays.
Source: GEO: Gene Expression Omnibus - February 6, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by array Homo sapiens Source Type: research

GSE196655 The role of MEF2A in iPSC-CMs maturation
Contributors : Jing Zhang ; Jingping Yang ; Zhaohui OuyangSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTo understand the role of MEF2A in iPSC-CMs maturation, we used MEF2A-siRNA to reduce MEF2A transcription in iPSC-CMs and then examined the changes in transcription levels
Source: GEO: Gene Expression Omnibus - February 3, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Genes, Vol. 14, Pages 398: Suppressors of Break-Induced Replication in Human Cells
Michael Leffak Short tandem DNA repeats are drivers of genome instability. To identify suppressors of break-induced mutagenesis human cells, unbiased genetic screens were conducted using a lentiviral shRNA library. The recipient cells possessed fragile non-B DNA that could induce DNA double-strand breaks (DSBs), integrated at an ectopic chromosomal site adjacent to a thymidine kinase marker gene. Mutagenesis of the thymidine kinase gene rendered cells resistant to the nucleoside analog ganciclovir (GCV). The screen identified genes that have established roles in DNA replication and repair, chromatin modification, re...
Source: Genes - February 3, 2023 Category: Genetics & Stem Cells Authors: Stanley Dean Rider French J. Damewood Rujuta Yashodhan Gadgil David C. Hitch Venicia Alhawach Resha Shrestha Matilyn Shanahan Nathen Zavada Michael Leffak Tags: Article Source Type: research

Acyclic (S)-Glycol Nucleic Acid (S-GNA) Modification of siRNAs Improves the Safety of RNAi Therapeutics While Maintaining Potency
RNA. 2023 Feb 1:rna.079526.122. doi: 10.1261/rna.079526.122. Online ahead of print.ABSTRACTGlycol nucleic acid (GNA) is an acyclic nucleic acid analogue connected via phosphodiester bonds. Crystal structures of RNA-GNA chimeric duplexes indicated that nucleotides of the right-handed (S)-GNA were better accommodated in the right-handed RNA duplex than were the left-handed (R)-isomers. GNA nucleotides adopt a rotated nucleobase orientation within all duplex contexts, pairing with complementary RNA in a reverse Watson-Crick mode, which explains the inabilities of GNA C and G to form strong base pairs with complementary nucleo...
Source: RNA - February 1, 2023 Category: Genetics & Stem Cells Authors: Martin Egli Mark K Schlegel Muthiah Manoharan Source Type: research

GSE222599 TMT-based quantitative proteomic analysis of spheroid cells of endometrial cancer possessing cancer stem cell properties
Contributors : Qingyan Zhang ; Mingzhu CaoSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensRNA-seq of HK2-siRNA transfected to Ishikawa and HEC1A cells to investigate the molecular mechanism of HK2 in endometrial cancer.
Source: GEO: Gene Expression Omnibus - January 14, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

A cellular senescence-related genes model allows for prognosis and treatment stratification of hepatocellular carcinoma: A bioinformatics analysis and experimental verification
Conclusion: We established a cellular senescence-based stratified model, and a multivariable-based nomogram, which could predict the survival of HCC patients and guide clinical treatment.
Source: Frontiers in Genetics - January 12, 2023 Category: Genetics & Stem Cells Source Type: research

GSE222499 Liver-specific FGFR4 knockdown in mice on a HFD increases bile acid synthesis and improves hepatic steatosis II
Contributor : Softic SamirSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusNonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease with increased risk in patients with metabolic syndrome. There are no FDA approved treatments, but farnesoid X receptor (FXR) agonists have shown promising results in clinical studies for NAFLD management. In addition to FXR,  fibroblast growth factor receptor FGFR4 is a key mediator of hepatic bile acid synthesis. Using N-acetylgalactosamine-conjugated siRNA, we knocked down FGFR4 specifically in the liver of mice on chow or...
Source: GEO: Gene Expression Omnibus - January 11, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research

GSE206845 Effect of depletion of USP36 on gene expression of ECA109 cells, esophageal squamous cell carcinoma cell line.
Contributors : Wenhao Zhang ; Xiaogang Zhao ; Jian ZhuSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTo investigate the function of USP36 in the regulation of cell proliferation and escc progression, we established eca109 cell lines in which each target gene has been knocked down by siRNA.We then performed gene expression profiling analysis using data obtained from RNA-seq of 6 different cells at same time points.
Source: GEO: Gene Expression Omnibus - January 4, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE217342 TSPAN18 facilitates bone metastasis of prostate cancer by protecting STIM1 from TRIM32-mediated ubiquitination
Contributors : Qianghua Zhou ; Tianxin Lin ; Hai HuangSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensWe demonstrated that TSPAN18 was overexpressed in prostate cancer (PCa) and markedly accelerated PCa cell migration and invasion in vitro and bone metastasis in vivo by inhibiting E3 ligase tripartite motif containing 32 (TRIM32)-mediated stromal interaction molecule ubiquitination and degradation. To further investigate the genes regulated by TSPAN18, we knockdown TSPAN18 by siRNA and performed high-throughput sequence. So we identify a series of genes regulated by TSPAN18.
Source: GEO: Gene Expression Omnibus - January 1, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE216708 Long non-coding RNA associated with mitogen-activated protein kinase in pancreatic cancer cells.
Contributors : Tomohiko Ishikawa ; Toru FurukawaSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensTo investigate the genes associated with LINC00941, we isolated total RNA from AsPC-1 with or without knockdown of LINC00941 using siRNA.Total transcriptome sequencing analysis was performed in total 3 samples.
Source: GEO: Gene Expression Omnibus - January 1, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

GSE207213 Effect of depletion of YTH family genes on gene expression in Nipponbera rices
Contributors : Weiwei Ma ; Xiaole WangSeries Type : Expression profiling by high throughput sequencingOrganism : Oryza sativaTo investigate the function YTH proteins in the regulation of gene expression, we established 12 transgenic lines in which each target gene has been knocked out by CRISPR/Cas9 or knocked down by siRNA.We then performed gene expression profiling analysis using data obtained from RNA-seq of 45 different transgenic lines.
Source: GEO: Gene Expression Omnibus - December 31, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Oryza sativa Source Type: research

GSE202053 Genome-wide distribution of chromatin accessibility and transcriptome of Set2-deficient Drosophila photoreceptor neurons
We report the chromatin accessibility landscape and transcriptome of Drosophila photoreceptor neurons that are Set2-deficient (via siRNA) obtained using a tissue-specific approach from 10-day old flies
Source: GEO: Gene Expression Omnibus - December 31, 2022 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing Drosophila melanogaster Source Type: research