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Total 371 results found since Jan 2013.

LMP2A inhibits the expression of KLF5 through the mTORC1 pathway in EBV-associated gastric carcinoma
CONCLUSION: LMP2A downregulated KLF5 expression by inhibiting the mTORC1 pathway in EBV positive GC cells. KLF5 might play an oncogene-like role by promoting the migration of GC cells and inducing autophagy.PMID:35500764 | DOI:10.1016/j.virusres.2022.198792
Source: Virus Research - May 2, 2022 Category: Virology Authors: Dandan Liu Duo Shi Lin Xu Lingling Sun Shuzhen Liu Bing Luo Source Type: research

Viruses, Vol. 14, Pages 657: HBsAg Loss as a Treatment Endpoint for Chronic HBV Infection: HBV Cure
ung Despite the availability of effective vaccines and antiviral therapy over the past two to three decades, chronic hepatitis B virus (HBV) infection remains a major global health threat as a leading cause of cirrhosis and liver cancer. Functional HBV cure defined as hepatitis B surface antigen (HBsAg) loss and undetectable serum HBV DNA is associated with improved clinical outcomes in patients with chronic HBV infection. However, spontaneous loss of HBsAg is rare and occurs in only 1% of all HBsAg-positive individuals annually. Furthermore, the rate of functional cure with currently available antiviral therapy is eve...
Source: Viruses - March 22, 2022 Category: Virology Authors: Maryam Moini Scott Fung Tags: Review Source Type: research

Importin KPNA2 confers HIV-1 pre-integration complex nuclear import by interacting with the capsid protein
In this study, we confirmed our previous RNA sequencing results and found that HIV-1 replication was significantly lower in 293T cells with siRNA-mediated KPNA2 knockdown and higher in KPNA2-upregulated cells. Quantitative PCR indicated that viral replication was impaired during cDNA nuclear import. The N-terminal of the capsid protein p24 interacted with KPNA2, and KPNB1 participated in KPNA2-mediated PIC nuclear import. Disruption of the capsid-KPNA2 binding by overexpression of full-length p24 or p24 N-terminal impaired the PIC nuclear import. These results indicate that KPNA2 is an important upstream adaptor of the KPN...
Source: Antiviral Research - March 18, 2022 Category: Virology Authors: Yanhui Song Hongguang Zhang Yinmiao Wang Jin Guo Shengjie Tang Lu Wang Ke Peng Chun-Sheng Dong Source Type: research

Identification of endoplasmic-reticulum-associated proteins involved in Bombyx mori nucleopolyhedrovirus entry by RNA-seq analysis
This study suggests that ERAPs may be involved in the BmNPV membrane fusion process during infection.
Source: Archives of Virology - February 24, 2022 Category: Virology Source Type: research

Complex Small RNA-mediated Regulatory Networks between Viruses/Viroids/Satellites and Host Plants
Virus Res. 2022 Feb 6:198704. doi: 10.1016/j.virusres.2022.198704. Online ahead of print.ABSTRACTHost plants deploy the small RNA (sRNA)-directed RNA silencing pathway to resist invasion by acellular microorganisms (viruses/viroids/satellites), and, in turn, this pathway is exploited by pathogenic agents to create an environment conducive to infection. Previous known sRNA-RNA systems consist of host endogenous microRNAs (miRNAs) mediating the regulation of host mRNAs and virus/viroid/satellite-derived small interfering RNAs (vsiRNAs) targeting their genomic RNAs. However, more in-depth explorations have substantially expan...
Source: Virus Research - February 9, 2022 Category: Virology Authors: Chaonan Wang Fan Jiang Shuifang Zhu Source Type: research

Viruses, Vol. 14, Pages 359: Encephalomyocarditis Virus 2A Protein Inhibited Apoptosis by Interaction with Annexin A2 through JNK/c-Jun Pathway
In this study, the 2A protein was expressed in Escherichia coli in order to find interacting cell proteins. A pull down assay, coupled with mass spectrometry, revealed that the 2A protein possibly interacted with annexin A2. Co-immunoprecipitation assays and confocal imaging analysis further demonstrated that the 2A protein interacted with annexin A2 in cells. In reducing the expression of annexin A2 by siRNA, the ability of the 2A protein to inhibit apoptosis was weakened and the proliferation of EMCV was slowed down. These results suggest that annexin A2 is closely related to the inhibition of apoptosis by 2A. Furthermor...
Source: Viruses - February 9, 2022 Category: Virology Authors: Ruochan Han Lin Liang Tong Qin Sa Xiao Ruiying Liang Tags: Article Source Type: research

Viruses, Vol. 14, Pages 339: In Vitro Inhibition of Replication of Dengue Virus Serotypes 1 & ndash;4 by siRNAs Bound to Non-Toxic Liposomes
The objective herein was to design and evaluate in vitro siRNAs bound to liposomes that inhibit DENV replication. siRNAs were designed against DENV1–4 from conserved regions using siDirect2.0 and Web-BLOCK-iT™ RNAiDesigner; the initial in vitro evaluation was carried out through transfection into HepG2 cells. siRNA with silencing capacity was encapsulated in liposomes composed of D-Lin-MC3-DMA, DSPC, Chol. Cytotoxicity, hemolysis, pro-inflammatory cytokine release and antiviral activity were evaluated using plaque assay and RT-qPCR. A working concentration of siRNA was established at 40 nM. siRN...
Source: Viruses - February 7, 2022 Category: Virology Authors: Rodriguez-Salazar Recalde-Reyes Bedoya Padilla-Sanabria Casta ño-Osorio Giraldo Tags: Article Source Type: research

TRIM26-mediated degradation of nucleocapsid protein limits porcine reproductive and respiratory syndrome virus-2 infection
Virus Res. 2022 Jan 22;311:198690. doi: 10.1016/j.virusres.2022.198690. Online ahead of print.ABSTRACTPorcine reproductive and respiratory syndrome (PRRS), caused by PRRSV, has ranked among the most economically important veterinary infectious diseases globally. Recently, tripartite motif (TRIMs) family members have arisen as novel restriction factors in antiviral immunity. Noteworthy, TRIM26 was reported as a binding partner of IRF3, TBK1, TAB1, and NEMO, yet its role in virus infection remains controversial. Herein, we showed that TRIM26 bound N protein by the C-terminal PRY/SPRY domain. Moreover, ectopic expression of T...
Source: Virus Research - January 25, 2022 Category: Virology Authors: Pandeng Zhao Huiyuan Jing Wang Dong Erzhen Duan Wenting Ke Ran Tao Yang Li Sufang Cao Haihua Wang Yan Zhang Yanting Sun Jinhe Wang Source Type: research

Viruses, Vol. 14, Pages 180: SGIV Induced and Exploited Cellular De Novo Fatty Acid Synthesis for Virus Entry and Replication
g Qiwei Qin Considerable attention has been paid to the roles of lipid metabolism in virus infection due to its regulatory effects on virus replication and host antiviral immune response. However, few literature has focused on whether lipid metabolism is involved in the life cycle of lower vertebrate viruses. Singapore grouper iridovirus (SGIV) is the causative aquatic virus that extensively causes fry and adult groupers death. Here, the potential roles of cellular de novo fatty acid synthesis in SGIV infection was investigated. SGIV infection not only increased the expression levels of key enzymes in fatty acid synt...
Source: Viruses - January 18, 2022 Category: Virology Authors: Qi Zheng Youhua Huang Liqun Wang Ya Zhang Xixi Guo Xiaohong Huang Qiwei Qin Tags: Article Source Type: research

Development of transgenic okra (Abelmoschus esculentus L. Moench) lines having RNA mediated resistance to Yellow vein mosaic virus (Geminiviridae)
J Virol Methods. 2022 Jan 6;301:114457. doi: 10.1016/j.jviromet.2022.114457. Online ahead of print.ABSTRACTBegomovirus Yellow vein mosaic virus causes severe yield losses in okra and even the resistant lines developed through conventional breeding show susceptibility at various levels. This paper describes the development of YVMV resistant lines through RNAi strategy. A universal ihpRNA construct harbouring βC1 ORF from the β-satellite of the begomovirus was designed using pRNAi-LIC plasmid. Complementarity checks in sequence databases had shown no off-target effects by the target region and the success of siRNA in inter...
Source: Journal of Virological Methods - January 9, 2022 Category: Virology Authors: Kelkar Vipul Ganesh Deepu Mathew Rehna Augustine Soni K B Swapna Alex Shylaja M R Anita Cherian K Source Type: research

Use of deep sequencing to profile small RNAs derived from tomato spotted wilt orthotospovirus and hippeastrum chlorotic ringspot orthotospovirus in infected Capsicum annuum
Virus Res. 2021 Dec 12:198648. doi: 10.1016/j.virusres.2021.198648. Online ahead of print.ABSTRACTVirus-derived small RNAs are one of the key factors of RNA silencing in plant defence against viruses. We obtained virus-derived small interfering RNA profiles from Tomato spotted wilt orthotospovirus and Hippeastrum chlorotic ringspot orthotospovirus infected Capsicum annuum XX19 and XY11 by deep sequencing one day after inoculation. The vsiRNAs data were mapped to the TSWV and HCRV genomes, and the results showed that the vsiRNAs measured 19-24 nucleotides in length. Most of the vsiRNAs were mapped to the S segment of the vi...
Source: Virus Research - December 15, 2021 Category: Virology Authors: Xue Gao Zhi-Qiang Jia Hong-Zheng Tao Ye Xu Yong-Zhong Li Ya-Ting Liu Source Type: research

Viruses, Vol. 13, Pages 2267: Major Vault Protein Inhibits Porcine Reproductive and Respiratory Syndrome Virus Infection in CRL2843CD163 Cell Lines and Primary Porcine Alveolar Macrophages
In this study, we evaluated the effect of MVP on PRRSV infection in CRL2843CD163 cell lines and porcine alveolar macrophages (PAMs). Our results showed that MVP expression was downregulated by PRRSV infection. Adenoviral overexpression of MVP inhibited PRRSV replication, whereas the siRNA knockdown of MVP promoted PRRSV replication. In addition, MVP knockdown has an adverse effect on the inhibitive role of MVP overexpression on PRRSV replication. Moreover, MVP could induce the expression of type Ⅰ IFNs and IFN-stimulated gene 15 (ISG15) in PRRSV-infected PAMs. Based on these results, MVP may be a potential molecular targ...
Source: Viruses - November 12, 2021 Category: Virology Authors: Xiaoping Wu Junyang Fang Qiuping Huang Xu Chen Zhongyi Guo Lingyujia Tian Enmin Zhou Jianxin Chen Yang Mu Taofeng Du Tags: Article Source Type: research