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Total 79 results found since Jan 2013.

Inhibition of human hepatocellular carcinoma tumor angiogenesis by siRNA silencing of VEGF via hepatic artery perfusion.
CONCLUSIONS: Our data demonstrated that VEGF silencing could suppress cells proliferation, promote cells apoptosis and reduce HCC angiogenesis through inactivation of VEGF/PI3K/AKT signaling pathway. PMID: 26744866 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - January 15, 2016 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research

Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL) Promotes Angiogenesis and Ischemia-Induced Neovascularization Via NADPH Oxidase 4 (NOX4) and Nitric Oxide-Dependent Mechanisms Coronary Heart Disease
Conclusions This is the first report demonstrating that TRAIL can promote angiogenesis following hindlimb ischemia in vivo. The angiogenic effect of TRAIL on human microvascular endothelial cell-1 cells is downstream of fibroblast growth factor-2, involving NOX4 and nitric oxide signaling. These data have significant therapeutic implications, such that TRAIL may improve the angiogenic response to ischemia and increase perfusion recovery in patients with cardiovascular disease and diabetes.
Source: JAHA:Journal of the American Heart Association - November 16, 2015 Category: Cardiology Authors: Di Bartolo, B. A., Cartland, S. P., Prado-Lourenco, L., Griffith, T. S., Gentile, C., Ravindran, J., Azahri, N. S. M., Thai, T., Yeung, A. W. S., Thomas, S. R., Kavurma, M. M. Tags: Coronary Heart Disease Source Type: research

Cardiomyocyte A Disintegrin And Metalloproteinase 17 (ADAM17) Is Essential in Post-Myocardial Infarction Repair by Regulating Angiogenesis Original Articles
Conclusions— This study highlights the key role of cardiomyocyte ADAM17 in post-MI recovery by regulating VEGFR2 transcription and angiogenesis, thereby limiting left ventricular dilation and dysfunction. Therefore, ADAM17 upregulation, within the physiological range, could provide protective effects in ischemic cardiomyopathy.
Source: Circulation: Heart Failure - September 15, 2015 Category: Cardiology Authors: Fan, D., Takawale, A., Shen, M., Wang, W., Wang, X., Basu, R., Oudit, G. Y., Kassiri, Z. Tags: Other heart failure, Angiogenesis, Cell signalling/signal transduction, Gene expression, Acute myocardial infarction Original Articles Source Type: research

Abstract 5222: Endothelial plasticity generates aberrant angiogenesis and therapy resistance in glioblastoma
Overgrown, abnormal vasculature characterizes the microenvironment that fuels cancer progression and induces therapeutic resistance. Here we reveal that endothelial plasticity drives excessive and abnormal tumor angiogenesis. Glioblastoma multiforme (GBM) is among the most lethal of human malignancies, distinguished by prominent vascularity and extreme vascular abnormality. We identify endothelial fibro-transformation (Endo-FT) in GBM, contributing significantly to aberrant vascularization, tumor progression, and therapeutic resistance. Utilizing human GBM specimens and allograft and genetic murine GBM models driven by RCA...
Source: Cancer Research - August 2, 2015 Category: Cancer & Oncology Authors: Huang, M., Fan, Y. Tags: Tumor Biology Source Type: research

Abstract 1265: LAMP2 overexpression in the plasma membrane of breast cancer cells in response of chronic acidosis as a new imaging and therapeutic target
A combination of poor vasculature perfusion, hypoxia, and increased flux of carbons through fermentative glycolysis lead to extracellular acidosis in solid tumors; with extracellular pH values as low as 6.5. The proton concentration increases within the lumen due to diffusion limitations and increased production of acid from hypoxic-glycolytic cells, causing the interior of the lumen to become highly acidic. The glycolytic phenotype can become “hardwired” as Warburg proposed, leading to the continued generation of metabolic acids even in well-oxygenated conditions. This acidified habitat is constant and imparts a Darwi...
Source: Cancer Research - August 2, 2015 Category: Cancer & Oncology Authors: Damaghi, M., Sprung, R., Tafreshi, N., Estrella, V., Koomen, J., Morse, D., Gillies, R. Tags: Molecular and Cellular Biology Source Type: research

Dopamine induces growth inhibition and vascular normalization through reprograming M2-polarized macrophages in rat C6 glioma.
Abstract Dopamine (DA), a monoamine catecholamine neurotransmitter with antiangiogenic activity, stabilizes tumor vessels in colon, prostate and ovarian cancer, thus increases chemotherapeutic efficacy. Here, in the rat C6 glioma models, we investigated the vascular normalization effects of DA and its mechanisms of action. DA (25, 50 mg/kg) inhibited tumor growth, while a precursor of DA (levodopa) prolonged the survival time of rats bearing orthotopic C6 glioma. DA improved tumor perfusion, with significant effects from day 3, and a higher level at day 5 to 7. In addition, DA decreased microvessel density and hyp...
Source: Toxicology and Applied Pharmacology - March 25, 2015 Category: Toxicology Authors: Qin T, Wang C, Chen X, Duan C, Zhang X, Zhang J, Chai H, Tang T, Chen H, Yue J, Li Y, Yang J Tags: Toxicol Appl Pharmacol Source Type: research

siRNA as a tool to improve the treatment of brain diseases: Mechanism, targets and delivery.
Abstract As the population ages, brain pathologies such as neurodegenerative diseases and brain cancer increase their incidence, being the need to find successful treatments of upmost importance. Drug delivery to the central nervous system (CNS) is required in order to reach diseases causes and treat them. However, biological barriers, mainly blood-brain barrier (BBB), are the key obstacles that prevent the effectiveness of possible treatments due to their ability to strongly limit the perfusion of compounds into the brain. Over the past decades, new approaches towards overcoming BBB and its efflux transporters ha...
Source: Ageing Research Reviews - March 18, 2015 Category: Genetics & Stem Cells Authors: Gomes MJ, Martins S, Sarmento B Tags: Ageing Res Rev Source Type: research

Pannexin 1 Channels Mediate the Release of ATP into the Lumen of the Rat Urinary Bladder.
This article is protected by copyright. All rights reserved. PMID: 25630792 [PubMed - as supplied by publisher]
Source: The Journal of Physiology - January 29, 2015 Category: Physiology Authors: Beckel JM, Daugherty SL, Tyagi P, Wolf-Johnston AS, Birder LA, Mitchell CH, de Groat WC Tags: J Physiol Source Type: research

Liver-specific Fas silencing prevents galactosamine/lipopolysaccharide-induced liver injury
Abstract Acute liver failure (ALF) is a life threatening disease for which only few treatment options exist. The molecular pathways of disease progression are not well defined, but the death receptor Fas (CD95/Apo-1) appears to play a pivotal role in hepatocyte cell death and the development of ALF. Here, we explored posttranscriptional gene silencing of Fas by RNAi to inhibit pathophysiological gene expression. For targeting Fas expression in mice, Fas siRNA was formulated with the liver-specific siRNA delivery system DBTC. Treatment of mice with DBTC/siRNAFas reduced Fas expression in the liver, but not in the ...
Source: Apoptosis - January 20, 2015 Category: Molecular Biology Source Type: research

Role of transcription factor CCAAT/enhancer‐binding protein alpha in human fetal liver cell types in vitro
ConclusionWe demonstrated that the effects of C/EBPα are specific for the different human fetal liver cell types, using an advanced 3‐D perfusion bioreactor as a human in vivo‐like model.
Source: Hepatology Research - October 9, 2014 Category: Internal Medicine Authors: Jörg C. Gerlach, Patrick Over, Hubert G. Foka, Morris E. Turner, Robert L. Thompson, Bruno Gridelli, Eva Schmelzer Tags: Original Article Source Type: research

Role ofTranscription Factor CCAAT/Enhancer Binding Protein Alphain Human Fetal LiverCell Typesin vitro
ConclusionsWe demonstrated that the effects of C/EBPα are specific for the different human fetal liver cell types, using an advanced three‐dimensional perfusion bioreactor as a humanin vivo‐like model.
Source: Hepatology Research - September 5, 2014 Category: Internal Medicine Authors: Jörg C. Gerlach, Patrick Over, Hubert G. Foka, Morris E. Turner, Robert L. Thompson, Bruno Gridelli, Eva Schmelzer Tags: Original Article Source Type: research

In Barrett's esophagus patients and Barrett's cell lines, ursodeoxycholic acid increases antioxidant expression and prevents DNA damage by bile acids
Hydrophobic bile acids like deoxycholic acid (DCA), which cause oxidative DNA damage and activate NF-B in Barrett's metaplasia, might contribute to carcinogenesis in Barrett's esophagus. We have explored mechanisms whereby ursodeoxycholic acid (UDCA, a hydrophilic bile acid) protects against DCA-induced injury in vivo in patients and in vitro using nonneoplastic, telomerase-immortalized Barrett's cell lines. We took biopsies of Barrett's esophagus from 21 patients before and after esophageal perfusion with DCA (250 μM) at baseline and after 8 wk of oral UDCA treatment. DNA damage was assessed by phospho-H2AX expression,...
Source: AJP: Gastrointestinal and Liver Physiology - July 15, 2014 Category: Gastroenterology Authors: Peng, S., Huo, X., Rezaei, D., Zhang, Q., Zhang, X., Yu, C., Asanuma, K., Cheng, E., Pham, T. H., Wang, D. H., Chen, M., Souza, R. F., Spechler, S. J. Tags: CALL FOR PAPERS Source Type: research

Sphingosine kinase 1 expressed by endothelial colony-forming cells has a critical role in their revascularization activity
Conclusion The up-regulation of SphK1 and S1P-dependent pathways is critical for the angiogenic/vasculogenic activity of ECFCs. The identification of this pathway provides attractive targets to optimize cell-based therapy for revascularization in ischaemic diseases.
Source: Cardiovascular Research - June 20, 2014 Category: Cardiology Authors: Poitevin, S., Cussac, D., Leroyer, A. S., Albinet, V., Sarlon-Bartoli, G., Guillet, B., Hubert, L., Andrieu-Abadie, N., Couderc, B., Parini, A., Dignat-George, F., Sabatier, F. Tags: Vascular biology Source Type: research

In Barrett's Esophagus Patients and Barrett's Cell Lines, Ursodeoxycholic Acid Increases Antioxidant Expression and Prevents DNA Damage by Bile Acids.
Abstract Hydrophobic bile acids like deoxycholic acid (DCA), which cause oxidative DNA damage and activate NF-κB in Barrett's metaplasia, might contribute to carcinogenesis in Barrett's esophagus. We have explored mechanisms whereby ursodeoxycholic acid (UDCA, a hydrophilic bile acid) protects against DCA-induced injury in vivo in patients and in vitro using non-neoplastic, telomerase-immortalized Barrett's cell lines. We took biopsies of Barrett's esophagus from 21 patients before and after esophageal perfusion with DCA (250 µM) at baseline, and after 8 weeks of oral UDCA treatment. DNA damage was assessed by p...
Source: Am J Physiol Gastroi... - May 22, 2014 Category: Gastroenterology Authors: Peng S, Huo X, Rezaei D, Zhang Q, Zhang X, Yu C, Asanuma K, Cheng E, Pham TH, Wang DH, Chen M, Souza RF, Spechler SJ Tags: Am J Physiol Gastrointest Liver Physiol Source Type: research

NDNF and Revascularization Signal Transduction
Strategies to stimulate revascularization are valuable for cardiovascular diseases. Here we identify neuron-derived neurotrophic factor (NDNF)/epidermacan as a secreted molecule that is up-regulated in endothelial cells in ischemic limbs of mice. NDNF was secreted from cultured human endothelial cells, and its secretion was stimulated by hypoxia. NDNF promoted endothelial cell network formation and survival in vitro through activation of Akt/endothelial NOS (eNOS) signaling involving integrin αvβ3. Conversely, siRNA-mediated knockdown of NDNF in endothelial cells led to reduction of cellular responses and basal Akt signa...
Source: Journal of Biological Chemistry - May 16, 2014 Category: Chemistry Authors: Ohashi, K., Enomoto, T., Joki, Y., Shibata, R., Ogura, Y., Kataoka, Y., Shimizu, Y., Kambara, T., Uemura, Y., Yuasa, D., Matsuo, K., Hayakawa, S., Hiramatsu-Ito, M., Murohara, T., Ouchi, N. Tags: Cell Biology Source Type: research