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Procedure: Ultrasound
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Total 92 results found since Jan 2013.
Novel cell-penetrating peptide-loaded nanobubbles synergized with ultrasound irradiation enhance EGFR siRNA delivery for triple negative Breast cancer therapy.
Abstract
The lack of safe and effective gene delivery strategies remains a bottleneck for cancer gene therapy. Here, we describe the synthesis, characterization, and application of cell-penetrating peptide (CPP)-loaded nanobubbles (NBs), which are characterized by their safety, strong penetrating power and high gene loading capability for gene delivery. An epidermal growth factor receptor (EGFR)-targeted small interfering RNA (siEGFR) was transfected into triple negative breast cancer (TNBC) cells via prepared CPP-NBs synergized with ultrasound-targeted microbubble destruction (UTMD) technology. Fluorescence micro...
Source: Colloids and Surfaces - June 20, 2016 Category: Biotechnology Authors: Jing H, Cheng W, Li S, Wu B, Leng X, Xu S, Tian J Tags: Colloids Surf B Biointerfaces Source Type: research
Small interfering RNA-loaded chitosan hydrochloride/carboxymethyl chitosan nanoparticles for ultrasound-triggered release to hamper colorectal cancer growth in vitro.
Abstract
Development of nontoxic, targetable and potent small interfering RNAs (siRNA) delivery systems remains a predominant challenge for clinical application of siRNA therapy. The nanoparticles of carboxymethyl chitosan (CMC) and labeled fluorescein isothiocyanate (FITC)-chitosan hydrochloride (CHC) were fabricated as carriers for ultrasound-triggered drug delivery to treat colon cancer. The results showed the (FITC-CHC)-CMC nanoparticles could effectively encapsulate anti-β-catenin siRNA through ionic gelation self-assembly to improve the stability of siRNA. The cumulative release ratio of siRNA from crosslin...
Source: International Journal of Biological Macromolecules - June 26, 2020 Category: Biochemistry Authors: Yan L, Gao S, Shui S, Liu S, Qu H, Liu C, Zheng L Tags: Int J Biol Macromol Source Type: research
Photoacoustic/ultrasound-guided gene silencing: Multifunctional microbubbles for treating adjuvant-induced arthritis
CONCLUSIONS: The theranostic MBs exhibited a TNF-α gene silencing effect under the guidance of CEUS and PAI. The theranostic MBs served as vehicles for delivering siRNA as well as contrast agents for CEUS and PAI.PMID:37012868 | DOI:10.1016/j.intimp.2023.109978
Source: International Immunopharmacology - April 4, 2023 Category: Allergy & Immunology Authors: Chenyang Zhao Rui Zhang Sirui Liu Xuelan Li Desheng Sun Yuxin Jiang Meng Yang Source Type: research
Murine portal vein catheterization to analyze liver-directed therapies
Conclusions: Liver-directed therapy can provide the selective delivery of siRNA to CRC metastases. EpCAM expression in CRC, but not normal liver, could further selectively target hepatic metastases of epithelial origin.
Source: Journal of Surgical Research - July 18, 2013 Category: Surgery Authors: Joseph D. Valentino, Piotr G. Rychahou, W. Conan Mustain, Victoria A. Elliott, B. Mark Evers Tags: Oncology/Endocrine Source Type: research
Chitosan nanoparticle carrying small interfering RNA to platelet-derived growth factor B mRNA inhibits proliferation of smooth muscle cells in rabbit injured arteries.
In conclusion, transfection of chitosan nanoparticle carrying siRNA against PDGF-B mRNA could inhibit proliferation of vSMCs in the rabbit iliac artery injury model.
PMID: 23518855 [PubMed - as supplied by publisher]
Source: Vascular - March 21, 2013 Category: Surgery Authors: Xia H, Jun J, Wen-Ping L, Yi-Feng P, Xiao-Ling C Tags: Vascular Source Type: research
Ultrasound-mediated vascular endothelial growth factor C (VEGF-C) gene microbubble transfection inhibits growth of MCF-7 breast cancer cells.
Abstract
We evaluated the effects of ultrasound-mediated microbubble transfection of VEGF-C siRNA on breast cancer cells in vitro and in vivo. MCF-7 cells were transfected with VEGF-C siRNA and the protein and mRNA expression of VEGF-C was tested using Western blot and qRT-PCR. Twenty nude mice tumors were established by injecting with MCF-7 cells, and were randomized into four groups when palpable tumors reached 190 mm3. The length and width of MCF-7 tumors in mice were measured every 3 days. After 20 days, all mice were killed and the expression of VEGF-C in tumor tissue was also detected by Western blot and qRT...
Source: Oncology Research - July 28, 2013 Category: Cancer & Oncology Authors: Xu Q, Sun T, Tian H, Wang C, Zhou H Tags: Oncol Res Source Type: research
Mechanism of programmed cell death factor 4/nuclear factor-{kappa}B signaling pathway in porcine coronary micro-embolization-induced cardiac dysfunction
In conclusion, PDCD4/NF-B signaling pathway activation is an important mechanism for CME-induced cardiac dysfunction, suggesting that inhibition of PDCD4/NF-B signaling pathway may be a potential target for the prevention and treatment of CME.
Source: Experimental Biology and Medicine - October 30, 2015 Category: Research Authors: Su, Q., Li, L., Wang, J., Zhou, Y., Liu, Y. Tags: Biochemistry & amp;amp; Molecular Biology Source Type: research
Silence of lncRNA XIST represses myocardial cell apoptosis in rats with acute myocardial infarction through regulating miR-449.
CONCLUSIONS: Knockdown of lncRNA XIST can repress the myocardial cell apoptosis in AMI model rats by downregulating miR-449 level.
PMID: 31646589 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - October 26, 2019 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
Ultrasound and Microbubble-Targeted Delivery of Small Interfering RNA Into Primary Endothelial Cells Is More Effective Than Delivery of Plasmid DNA
Abstract: Ultrasound and microbubble-targeted delivery (UMTD) is a promising non-viral technique for genetic-based therapy. We found that UMTD of small interfering RNA (siRNA) is more effective than delivery of plasmid DNA (pDNA). UMTD (1 MHz, 0.22 MPa) of fluorescently labeled siRNA resulted in 97.9 ± 1.5% transfected cells, with siRNA localized homogenously in the cytoplasm directly after ultrasound exposure. UMTD of fluorescently labeled pDNA resulted in only 43.0 ± 4.2% transfected cells, with localization mainly in vesicular structures, co-localizing with endocytosis markers clathrin and caveolin. Delivery of siRNA ...
Source: Ultrasound in Medicine and Biology - December 23, 2013 Category: Radiology Authors: Lynda J.M. Juffermans, Bernadet D.M. Meijering, Robert H. Henning, Leo E. Deelman Tags: Original Contributions Source Type: research
Acoustic Cavitation-Mediated Delivery of Small Interfering Ribonucleic Acids with Phase-Shift Nano-emulsions
Localized, targeted delivery of small interfering ribonucleic acid (siRNA) has been the foremost hurdle in the use of siRNA for the treatment of various diseases. Major advances have been achieved in the synthesis of siRNA, which have led to greater target messenger RNA (mRNA) silencing and stability under physiologic conditions. Although numerous delivery strategies have shown promise, there are still limited options for targeted delivery and release of siRNA administered systemically. In this in vitro study, phase-shift nano-emulsions (PSNE) were explored as cavitation nuclei to facilitate free siRNA delivery to cancer ...
Source: Ultrasound in Medicine and Biology - May 13, 2015 Category: Radiology Authors: Mark T. Burgess, Tyrone M. Porter Tags: Original Contribution Source Type: research
Nucleic Acid Delivery System by the Combination of Lipid bubbles and Ultrasound.
CONCLUSION: Ultrasound technology is widely used in clinical settings not only for diagnosis but also for therapy. Ultrasonic devices are being actively developed. Computer-controlled ultrasound systems can provide precise exposure to the target site. The combination of precise ultrasound exposure and LBs might be useful for target site-specific nucleic acids delivery, and holds potential to be developed into a beneficial therapeutic and diagnostic system for various diseases.
PMID: 30084324 [PubMed - as supplied by publisher]
Source: Current Pharmaceutical Design - August 7, 2018 Category: Drugs & Pharmacology Authors: Endo-Takahashi Y, Maruyama K, Negishi Y Tags: Curr Pharm Des Source Type: research
Downregulation of Renal G Protein-Coupled Receptor Kinase Type 4 Expression via Ultrasound-Targeted Microbubble Destruction Lowers Blood Pressure in Spontaneously Hypertensive Rats Hypertension
ConclusionsTaken together, these study results indicate that UTMD‐targeted GRK4 siRNA delivery to the kidney effectively reduces D1R phosphorylation by inhibiting renal GRK4 expression, improving D1R‐mediated natriuresis and diuresis, and lowering BP, which may provide a promising novel strategy for gene therapy for hypertension.
Source: JAHA:Journal of the American Heart Association - October 5, 2016 Category: Cardiology Authors: Huang, H., Li, X., Zheng, S., Chen, Y., Chen, C., Wang, J., Tong, H., Zhou, L., Yang, J., Zeng, C. Tags: Nephrology and Kidney, High Blood Pressure, Hypertension Original Research Source Type: research
LncRNA NEAT1 alleviates sepsis-induced myocardial injury by regulating the TLR2/NF- κB signaling pathway.
CONCLUSIONS: NEAT1 knockdown can improve LPS-induced myocardial injury in mice by inhibiting the TLR2/NF-κB signaling pathway. LncRNA NEAT1 is expected to be a potential target for clinical treatment of the sepsis-induced myocardial injury.
PMID: 31210324 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - June 20, 2019 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
CircRNA 010567 improves myocardial infarction rats through inhibiting TGF- β1.
CONCLUSIONS: CircRNA 010567 siRNA can improve the cardiac function, alleviate the MF, and inhibit the myocardial apoptosis, thereby further suppressing MI-induced MF, whose mechanism may be related to the inhibition on the TGF-β1 signaling pathway.
PMID: 31957851 [PubMed - in process]
Source: European Review for Medical and Pharmacological Sciences - January 21, 2020 Category: Drugs & Pharmacology Tags: Eur Rev Med Pharmacol Sci Source Type: research
