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Procedure: Ultrasound
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Total 92 results found since Jan 2013.
A new method of ultrasonic nonviral gene delivery to the adult myocardium
Abstract: Cardiac gene transfer is a powerful molecular tool to improve our understanding of the role of new proteins and mutants in cardiac pathophysiology. There is a need for a simple efficient myocardial gene delivery technique in order to study the physiological role of proteins in their native environment. Here we tested a new method of myocardial nonviral gene delivery, by using the combination of ultrasound energy (USE), liposomes and high pressure injections to the rat heart. Wistar rats were subjected to intra-myocardial injections of liposomes-DNA or siRNA mix. The heart was exposed after an inter-costal incisio...
Source: Journal of Molecular and Cellular Cardiology - August 6, 2012 Category: Cytology Authors: Youakim Saliba, Nathalie Mougenot, Adeline Jacquet, Fabrice Atassi, Stéphane Hatem, Nassim Farès, Anne-Marie Lompré Tags: Original Articles Source Type: research
Low-intensity pulsed ultrasound prevents prolonged hypoxia-induced cardiac fibrosis through HIF-1 α/DNMT3a pathway via a TRAAK-dependent manner
This study aimed to determine whether low-intensity pulsed ultrasound (LIPUS), a novel and safe apparatus, could alleviate hypoxia-induced cardiac fibrosis, and to elucidate the underlying mechanisms. Hypoxia (1% O2 ) and transverse aortic constriction (TAC) were performed on neonatal rat cardiac fibroblasts and mice to induce cardiac fibrosis, respectively. LIPUS irradiation was applied for 20 minutes every 6 hours for a total of 2 times in vitro, and every 2 days from 1 week before surgery to 4 weeks after surgery in vivo. We found that LIPUS dose-dependently attenuated hypoxia-induced cardiac fibroblast phenotypic conve...
Source: Clinical and Experimental Pharmacology and Physiology - August 3, 2021 Category: Drugs & Pharmacology Authors: Kun Zhao Liqing Weng Tianhua Xu Chuanxi Yang Jing Zhang Gehui Ni Xiasheng Guo Juan Tu Dong Zhang Wei Sun Xiangqing Kong Source Type: research
Ultrasound-triggered microbubble destruction enhances the radiosensitivity of glioblastoma by inhibiting PGRMC1-mediated autophagy in vitro and in vivo
ConclusionUTMD enhanced the radiosensitivity of glioblastoma partially by disrupting PGRMC1-mediated autophagy.
Source: Military Medical Research - February 14, 2022 Category: International Medicine & Public Health Source Type: research
Exploring the relationship between autophagy and Gefitinib resistance in NSCLC by silencing PDLIM5 using ultrasound-targeted microbubble destruction technology
This study investigates novel ultrasound (US) sensitive siRNA-loaded nanobubbles (siRNA-NBs) to explore t...
Source: Cancer Cell International - September 25, 2022 Category: Cancer & Oncology Authors: Yao Zhang, Wenhao Lv, Hui Li, Tiantian Dong, Hao Wu, Chunhong Su, Hong Shu and Fang Nie Tags: Research Source Type: research
Enhanced siRNA Delivery Using a Combination of an
Arginine-Grafted Bioreducible Polymer, Ultrasound, and Microbubbles
in Cancer Cells
Molecular PharmaceuticsDOI: 10.1021/mp400048p
Source: Molecular Pharmaceutics - April 5, 2013 Category: Drugs & Pharmacology Authors: Stelios Florinas, Hye Yeong Nam and Sung Wan Kim Source Type: research
Preparation of Protamine Cationic Nanobubbles and Experimental Study of Their Physical Properties and In Vivo Contrast Enhancement
In this study, we aimed to prepare a novel type of microbubble (MB), protamine cationic nanobubble (NB), to provide a new vector for tumor gene therapy. We prepared cationic NBs with protamine and other lipid components using mechanical oscillation. The protamine cationic NBs had a mean diameter of 521.2 ± 37.57 nm, a zeta potential of +18.5 mV, and a gene-carrying capacity of 15.69 μg androgen receptor (AR) siRNA per 108 NBs. The cationic NBs exhibited superior contrast enhancement for in vivo imaging compared with SonoVue (Bracco, Geneva, Switzerland), and their physical properties did not change significantly a...
Source: Ultrasound in Medicine and Biology - August 12, 2013 Category: Radiology Authors: Hai-Peng Tong, Luo-Fu Wang, Yan-Li Guo, Lang Li, Xiao-Zhou Fan, Jun Ding, Hai-Yun Huang Tags: Original Contributions Source Type: research
Rap1 ameliorates renal tubular injury in diabetic nephropathy.
Abstract
Rap1b ameliorates high glucose (HG)-induced mitochondrial dysfunction in tubular cells. However, its role and precise mechanism in diabetic nephropathy (DN) in vivo remains unclear. We hypothesize that Rap1 plays a protective role in tubular damage of DN by modulating primarily the mitochondria-derived oxidative stress.The role and precise mechanisms of Rap1b on mitochondrial dysfunction and of tubular cells in DN was examined in rats with Streptozotocin (STZ)-induced diabetes that have Rap1b gene transfer using an ultrasound microbubble-mediated technique as well as in renal proximal epithelial tubular c...
Source: Diabetes - December 18, 2013 Category: Endocrinology Authors: Xiao L, Zhu X, Yang S, Liu F, Zhou Z, Zhan M, Xie P, Zhang D, Li J, Song P, Kanwar YS, Sun L Tags: Diabetes Source Type: research
TXNIP-NLRP3 Binding in Podocyte Inflammasomes Metabolism
In conclusion, our results demonstrate that TXNIP binding to NLRP3 is a key signaling mechanism necessary for hHcys-induced NLRP3 inflammasome formation and activation and subsequent glomerular injury.
Source: Journal of Biological Chemistry - September 25, 2014 Category: Chemistry Authors: Abais, J. M., Xia, M., Li, G., Chen, Y., Conley, S. M., Gehr, T. W. B., Boini, K. M., Li, P.-L. Tags: Molecular Bases of Disease Source Type: research
Inhibition of VDAC1 prevents Ca 2+ -mediated oxidative stress and apoptosis induced by 5-aminolevulinic acid mediated sonodynamic therapy in THP-1 macrophages
Abstract
Ultrasound combined with endogenous protoporphyrin IX derived from 5-aminolevulinic acid (ALA-SDT) is known to induce apoptosis in multiple cancer cells and macrophages. Persistent retention of macrophages in the plaque has been implicated in the pathophysiology and progression of atherosclerosis. Here we investigated the effects of inhibition of voltage-dependent anion channel 1 (VDAC1) on ALA-SDT-induced THP-1 macrophages apoptosis. Cells were pre-treated with VDAC1 inhibitor 4,4′-diisothiocyanostilbene-2,2′-disulfonic acid (DIDS) disodium salt for 1 h or downregulated VDAC1 expression by small int...
Source: Apoptosis - October 23, 2014 Category: Molecular Biology Source Type: research
Abstract 2787: Modulation of autophagy and oncogenic phenotypes through CYB5A-TRAF6 signaling influence prognosis of resected and metastatic pancreatic cancer
High-resolution array comparative genomic hybridization in a cohort of 44 radically resected pancreatic cancer patients revealed a correlation between shorter survival and loss of the 18q22.3cytoband. The present study investigated the genes encoded by this cytoband and provided mechanistic insights on their role in the suppression of oncogenic properties. We studied mRNA and protein expression of FBXO15, C18orf55, CYB5A, CPGL and CPGL-B in frozen laser-microdissected tissues from radically resected (N=48) and biopsies from metastatic patients (N=50). Low CYB5A expression correlated with 18q22.3 deletion and shorter surviv...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Giovannetti, E., Wang, Q., Funel, N., Avan, A., Lagerweij, T., Caretti, V., Boggi, U., Wang, Y., Caponi, S., Velde, A. v. d., Vasile, E., Verheul, H. M., Wurdinger, T., Giaccone, G. Tags: Experimental and Molecular Therapeutics Source Type: research
Abstract C42: Quantitative high-throughput efficacy profiling of hedgehog/GLI pathway antagonists in inflammatory breast cancer
Conclusion: Several agents showed efficacy in in vitro IBC cancer models demonstrating that GLI inhibitors may be a valid therapeutic approach for targeting GLI-dependent IBC cancers.Funded in part by DOD/CDMRP IDEA W81XWH-13-1-0141(BC121850) (KP Williams)[1] W.F. Anderson, C. Schairer, B.E. Chen, K.W. Hance, P.H. Levine, Epidemiology of inflammatory breast cancer (IBC). Breast disease 22 (2006) 9-23.[2] W.A. Woodward, M. Cristofanilli, Inflammatory breast cancer. Seminars in Radiation Oncology 19 (2009) 256-265.[3] K.A. Hirko, A.S. Soliman, M. Banerjee, J. Ruterbusch, J.B. Harford, R.M. Chamberlain, J.J. Graff, S.D. Meraj...
Source: Cancer Epidemiology Biomarkers and Prevention - November 13, 2014 Category: Cancer & Oncology Authors: Oladapo, H., Ingram, S., Stefanowicz, A., Devi, G., Williams, K. Tags: Proteomics, Chemogenomics, and Chemoinformatics: Poster Presentations - Proffered Abstracts Source Type: research
Gene interference strategies as a new tool for the treatment of prostate cancer
Abstract
Prostate cancer (PCa) is one of the most common cancer in men. It affects older men and the incidence increases with age; the median age at diagnosis is 67 years. The diagnosis of PCa is essentially based on three tools: digital rectal exam, serum concentration of prostate specific antigen, and transrectal ultrasound-guided biopsy. Currently, the therapeutic treatments of this cancer are different and range from the prostatectomy to hormonal therapy, to radiation therapy, to immunotherapy, and to chemotherapy. However, additional efforts are required in order to find new weapons for the treatment of meta...
Source: Endocrine - June 7, 2015 Category: Endocrinology Source Type: research
Multifunctional PLGA Nanobubbles as Theranostic Agents: Combining Doxorubicin and P-gp siRNA Co-Delivery Into Human Breast Cancer Cells and Ultrasound Cellular Imaging
Source: Journal of Biomedical Nanotechnology - August 1, 2015 Category: Nanotechnology Authors: Yang, HongDeng, LiweiLi, TingtingShen, XueYan, JieZuo, LiangmingWu, ChunhuiLiu, Yiyao Tags: Articles Source Type: research
Increased efficiency of testicular tumor chemotherapy by ultrasound microbubble-mediated targeted transfection of siMDR1.
Authors: He Y, Bi Y, Ji XJ, Wei G
Abstract
The MDR1 gene encoding P-glycoprotein (P-gp) is an ATP-dependent drug efflux transporter and is related to drug resistance of yolk sac tumors. Drug resistence may be an important factor for the low efficiency of chemotherapy in the treatment of testicular tumors. P-gp, encoded by the MDR1 gene, is an ATP-binding cassette transporter. P-gp exhibits high expression in capillary endothelial cells of the testis and prevents the intracellular accumulation of chemotherapy agents in testicular tumor cells, resulting in drug resistance. In the present study, we aimed to use specif...
Source: Oncology Reports - September 11, 2015 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research
