A knock-in mutation at cysteine 144 of TRIM72 is cardioprotective and reduces myocardial TRIM72 release
TRIM72 is a membrane repair protein that protects against ischemia reperfusion (I/R) injury. We previously identified Cys144 (C144) on TRIM72 as a site of S-nitrosylation. To study the importance of C144, we generated a knock-in mouse with C144 mutated to a serine (TRIM72 C144S). We subjected ex vivo perfused mouse hearts to 20  min of ischemia followed by 90 min of reperfusion and observed less injury in TRIM72 C144S compared to WT hearts. Infarct size was smaller (54 vs 27% infarct size) and cardiac functional recovery (37 vs 62% RPP) was higher for the TRIM72 C144S mouse hearts. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - September 16, 2019 Category: Cytology Authors: Natasha Fillmore, Kevin M. Casin, Prithvi Sinha, Junhui Sun, Hanley Ma, Jennifer Boylston, Audrey Noguchi, Chengyu Liu, Nadan Wang, Guangshuo Zhou, Mark J. Kohr, Elizabeth Murphy Source Type: research

Cardiac expression of the microsomal triglyceride transport protein protects the heart function during ischemia
The microsomal triglyceride transport protein (MTTP) is critical for assembly and secretion of apolipoprotein B (apoB)-containing lipoproteins and is most abundant in the liver and intestine. Surprisingly, MTTP is also expressed in the heart. Here we tested the functional relevance of cardiac MTTP expression. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - September 15, 2019 Category: Cytology Authors: Martina Klevstig, Muhammad Arif, Maria Mannila, Sara Svedlund, Ismena Mardani, Marcus St åhlman, Linda Andersson, Malin Lindbom, Azra Miljanovic, Anders Franco-Cereceda, Per Eriksson, Anders Jeppsson, Li-Ming Gan, Malin Levin, Adil Mardinoglu, Ewa Ehrenb Source Type: research

Cardiotoxicity screening of illicit drugs and new psychoactive substances (NPS) in human iPSC-derived cardiomyocytes using microelectrode array (MEA) recordings
The use of recreational drugs, including new psychoactive substances (NPS), is paralleled by emergency department visits of drug users with severe cardiotoxicity. Drug-induced cardiotoxicity can be the (secondary) result of increased norepinephrine blood concentrations, but data on potential drug-induced direct effects on cardiomyocyte function are scarce. The presence of hundreds of NPS therefore calls for efficient screening models to assess direct cardiotoxicity.We investigated effects of four reference compounds (3 –30 nM dofetilide, nifedipine and isoproterenol, and 1–10 μM mexiletine) and six recre...
Source: Journal of Molecular and Cellular Cardiology - September 14, 2019 Category: Cytology Authors: Anne Zwartsen, Tessa de Korte, Peter Nacken, Dylan W. de Lange, Remco H.S. Westerink, Laura Hondebrink Source Type: research

Corrigendum to “Cilostazol ameliorates heart failure with preserved ejection fraction and diastolic dysfunction in obese and non-obese hypertensive mice” [J. Mol. Cell. Cardiol. 123 (2018) 46–57]
The authors regret for an inadvertent formatting error which led to the pasting of the wrong graph for the EDV parameter (Fig. 2 panel D). Therefore, the readers are requested to consider the following correct graph for the EDV parameter (Fig. 2 Panel D) instead of the one shown in the published article. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - September 12, 2019 Category: Cytology Authors: Sukka Santosh Reddy, Heena Agarwal, Manoj Kumar Barthwal Tags: Corrigendum Source Type: research

Comparative study on isolation and mitochondrial function of adult mouse and rat cardiomyocytes
Cultured adult mouse and rat cardiomyocytes are the best and low-cost cell model for cardiac cellular physiology, pathology, drug toxicity screening, and intervention. The functions of mouse cardiomyocytes decline faster than rat cardiomyocytes in culture conditions. However, little is known about the difference of mitochondrial function between cultured mouse and rat myocytes. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - September 12, 2019 Category: Cytology Authors: Bilin Liu, Anqi Li, Yuan Qin, Xiangang Tian, Meng Gao, Wenting Jiang, Guohua Gong Source Type: research

The axis of local cardiac endogenous Klotho-TGF- β1-Wnt signaling mediates cardiac fibrosis in human
This study aimed to further clarify endogenous Klotho's functional roles in cardiac fibrosis in patients with underlying CKD. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - September 11, 2019 Category: Cytology Authors: Qinghua Liu, Lang-Jing Zhu, Ana Maria Waaga-Gasser, Yan Ding, Minghua Cao, Shreyas J. Jadhav, Sandra Kirollos, Prem S. Shekar, Robert F. Padera, Yu-Chun Chang, Xingbo Xu, Elisabeth M. Zeisberg, David M. Charytan, Li-Li Hsiao Source Type: research

Carbonic anhydrase II/sodium-proton exchanger 1 metabolon complex in cardiomyopathy of ob −/− type 2 diabetic mice
Heart failure is the leading cause of death among diabetic people. Cellular and molecular entities leading to diabetic cardiomyopathy are, however, poorly understood. Coupling of cardiac carbonic anhydrase II (CAII) and Na+/H+ exchanger 1 (NHE1) to form a transport metabolon was analyzed in obese type 2 diabetic mice (ob −/−) and control heterozygous littermates (ob+/−). Echocardiography showed elevated systolic interventricular septum thickness and systolic posterior wall thickness in ob−/− mice at 9 and 16 weeks. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - September 10, 2019 Category: Cytology Authors: Carolina Jaquenod de Giusti, Paula G. Blanco, Paula A. Lamas, Fernanda Carrizo Velasquez, Juan M. Lofeudo, Enrique L. Portiansky, Bernardo V. Alvarez Source Type: research

Molecular machinery and interplay of apoptosis and autophagy in coronary heart disease
Coronary heart disease (CHD) is a common heart disease and the leading cause of cardiovascular death. Apoptosis and autophagy are two forms of programmed cell deaths which participate in the pathogenesis, development and prognosis of CHD. They are activated by several different pathways respectively and can interact with each other through the Beclin 1-Bcl-2/Bcl-xL complex, mTOR, TRAIL, TNF- α, ER stress and nucleus p53 pathways. Excessive apoptosis can promote myocardial ischemia, ischemia/reperfusion (I/R) injury, post-ischemia cardiac remodeling and coronary atherosclerosis except for VSMC-induced atherosclerosis ...
Source: Journal of Molecular and Cellular Cardiology - September 7, 2019 Category: Cytology Authors: Yan Dong, Hengwen Chen, Jialiang Gao, Yongmei Liu, Jun Li, Jie Wang Source Type: research

The evolutionarily conserved C-terminal peptide of troponin I is an independently configured regulatory structure to function as a myofilament Ca2+-desensitizer
The C-terminal end segment of troponin subunit I (TnI) is a structure highly conserved among the three muscle type-specific isoforms and across vertebrate species. Partial deletion or point mutation in this segment impairs cardiac muscle relaxation. In the present study, we characterized the C-terminal 27 amino acid peptide of human cardiac TnI (HcTnI-C27) for its role in modulating muscle contractility. Biologically or chemically synthesized HcTnI-C27 peptide retains an epitope structure in physiological solutions similarly to that in intact TnI as recognized by an anti-TnI C-terminus monoclonal antibody (mAb TnI-1). (Sou...
Source: Journal of Molecular and Cellular Cardiology - September 7, 2019 Category: Cytology Authors: Sienna Wong, Han-Zhong Feng, J.-P. Jin Source Type: research

MicroRNA-125a-3p affects smooth muscle cell function in vascular stenosis
Many studies have indicated that microRNAs are closely related to the process of peripheral arterial disease (PAD). Previously, we found that microRNA-125a-3p (miR-125a-3p) in restenotic arteries after interventional therapy of lower extremity vessels was notably decreased compared with that of normal control arteries. However, its role in the development of vascular stenosis is not yet clearly understood. The purpose of this study was to investigate the expression, regulatory mechanism and function of miR-125a-3p in the process of vascular stenosis. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - September 6, 2019 Category: Cytology Authors: Wei Hu, Guangqi Chang, Mao Zhang, Yuxi Li, Lepin Yin, Youjin Huang, Chao Feng, Yi Gu, Dacui Wen, Shenming Wang Source Type: research

Intrafibrillar and perinuclear mitochondrial heterogeneity in adult cardiac myocytes
Mitochondria are involved in multiple cellular functions, in addition to their core role in energy metabolism. Mitochondria localized in different cellular locations may have different morphology, Ca2+ handling and biochemical properties and may interact differently with other intracellular structures, causing functional specificity. However, most prior studies have utilized isolated mitochondria, removed from their intracellular environment. Mitochondria in cardiac ventricular myocytes are highly organized, with a majority squeezed between the myofilaments in longitudinal chains (intrafibrillar mitochondria, IFM). (Source...
Source: Journal of Molecular and Cellular Cardiology - September 3, 2019 Category: Cytology Authors: Xiyuan Lu, Phung N. Thai, Shan Lu, Jun Pu, Donald M. Bers Source Type: research

Death receptor 5 contributes to cardiomyocyte hypertrophy through epidermal growth factor receptor transactivation
Cardiomyocyte survival and death contributes to many cardiac diseases. A common mechanism of cardiomyocyte death is through apoptosis however, numerous death receptors (DR) have been virtually unstudied in the context of cardiovascular disease. Previous studies have identified TNF-related apoptosis inducing ligand (TRAIL) and its receptor, DR5, as being altered in a chronic catecholamine administration model of heart failure, and suggest a role of non-canonical signaling in cardiomyocytes. Furthermore, multiple clinical studies have identified TRAIL or DR5 as biomarkers in the prediction of severity and mortality following...
Source: Journal of Molecular and Cellular Cardiology - August 29, 2019 Category: Cytology Authors: Miles A. Tanner, Toby P. Thomas, Laurel A. Grisanti Source Type: research

Cardioprotective effects of idebenone do not involve ROS scavenging: Evidence for mitochondrial complex I bypass in ischemia/reperfusion injury
In this study, we determined the cardiac and mitochondrial effects of idebenone, a benzoquinone currently in several clinical trials with purported ‘antioxidant’ effects. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - August 22, 2019 Category: Cytology Authors: Justin B. Perry, Grace N. Davis, Mitchell E. Allen, Marina Makrecka-Kuka, Maija Dambrova, Robert W. Grange, Saame Raza Shaikh, David A. Brown Source Type: research

Doxorubicin induces cardiomyocyte pyroptosis via the TINCR-mediated posttranscriptional stabilization of NLR family pyrin domain containing 3
In this study, we sought to identify the effect of DOX on cardiomyocyte pyroptosis and further reveal the underlying regulatory mechanism. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - August 21, 2019 Category: Cytology Authors: Liping Meng, Hui Lin, Jie Zhang, Na Lin, Zhenzhu Sun, Feidan Gao, Hangqi Luo, Tingjuan Ni, Wenqiang Luo, Jufang Chi, Hangyuan Guo Source Type: research

Mechanical strain induces a pro-fibrotic phenotype in human mitral valvular interstitial cells through RhoC/ROCK/MRTF-A and Erk1/2 signaling pathways
The mitral valve is a complex multilayered structure populated by fibroblast-like cells, valvular interstitial cells (VIC) which are embedded in an extracellular matrix (ECM) scaffold and are submitted to the mechanical deformations affecting valve at each heartbeat, for an average of 40 million times per year. Myxomatous mitral valve (MMV) is the most frequent heart valve disease characterized by disruption of several valvular structures due to alterations of their ECM preventing the complete closure of the valve resulting in symptoms of prolapse and regurgitation. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - August 20, 2019 Category: Cytology Authors: Benoit Blomme, Christophe Deroanne, Alexia Hulin, Charles Lambert, Jean-Olivier Defraigne, Betty Nusgens, Marc Radermecker, Alain Colige Source Type: research

Large scale, unbiased analysis of elementary calcium signaling events in cardiac myocytes
The identification of spatiotemporally restricted Ca2+ signals, Ca2+ sparks, was instrumental for our understanding of cardiac Ca2+ homeostasis. High-speed 2D confocal imaging enables acquisition of such Ca2+ sparks with high-content information but their full appreciation is constrained by the lack of unbiased and easy-to-use analysis tools. We developed a software toolset for unbiased and automatic Ca2+ spark analysis for huge data sets of subcellular Ca2+ signals. iSpark was developed to be scanner and detector independent. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - August 13, 2019 Category: Cytology Authors: Qinghai Tian, Laura Schr öder, Yvonne Schwarz, Aline Flockerzi, Lars Kaestner, Andre Zeug, Dieter Bruns, Peter Lipp Source Type: research

Hypoplastic left heart syndrome: From bedside to bench and back
Hypoplastic Left Heart Syndrome (HLHS) is a complex Congenital Heart Disease (CHD) that was almost universally fatal until the advent of the Norwood operation in 1981. Children with HLHS who largely succumbed to the disease within the first year of life, are now surviving to adulthood. However, this survival is associated with multiple comorbidities and HLHS infants have a higher mortality rate as compared to other non-HLHS single ventricle patients. In this review we (a) discuss current clinical challenges associated in the care of HLHS patients, (b) explore the use of systems biology in understanding the molecular framew...
Source: Journal of Molecular and Cellular Cardiology - August 13, 2019 Category: Cytology Authors: Anita Saraf, Wendy Book, Timothy J. Nelson, Chunhui Xu Tags: Review article Source Type: research

Tachycardia-induced CD44/NOX4 signaling is involved in the development of atrial remodeling
This study further evaluated whether CD44 and its related signaling mediate atrial tachycardia-induced oxidative stress and Ca2+-handling abnormalities. Tachypacing in atrium-derived myocytes (HL-1 cell line) induced the activation of CD44-related signaling, including HA and HA synthase (HAS) expression. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - August 13, 2019 Category: Cytology Authors: Wei-Jan Chen, Shang-Hung Chang, Yi-Hsin Chan, Jia-Lin Lee, Ying-Ju Lai, Gwo-Jyh Chang, Feng-Chun Tsai, Yung-Hsin Yeh Source Type: research

A small-molecule LF3 abrogates β-catenin/TCF4-mediated suppression of NaV1.5 expression in HL-1 cardiomyocytes
Increased nuclear β-catenin interacting with T-cell factor 4 (TCF4) affects the expression of target genes including SCN5A in ischemic heart disease, which is characterized by frequent ventricular tachycardia/fibrillation. A complex of β-catenin and TCF4 inhibits cardiac Na+ channel activity by reducing NaV1.5 expr ession through suppressing SCN5A promoter activity in HL-1 cardiomyocytes. LF3, a 4-thioureido-benzenesulfonamide derivative and an inhibitor of β-catenin/TCF4 interaction, has been shown to block the self-renewal capacity of cancer stem cells. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - August 13, 2019 Category: Cytology Authors: Limei Zhao, Lihua Sun, Yan Lu, Faqian Li, Haodong Xu Source Type: research

EZH2 as a novel therapeutic target for atrial fibrosis and atrial fibrillation
Angiotensin II (Ang-II)-induced fibroblast differentiation plays an important role in the development of atrial fibrosis and atrial fibrillation (AF). Here, we show that the expression of the histone methyltransferase enhancer of zeste homolog 2 (EZH2) is increased in atrial muscle and atrial fibroblasts in patients with AF, accompanied by significant atrial fibrosis and atrial fibroblast differentiation. In addition, EZH2 is induced in murine models of atrial fibrosis. Furthermore, either pharmacological GSK126 inhibition or molecular silencing of EZH2 can inhibit the differentiation of atrial fibroblasts and the ability ...
Source: Journal of Molecular and Cellular Cardiology - August 10, 2019 Category: Cytology Authors: Shuai Song, Rui Zhang, Binfeng Mo, Long Chen, Liang Liu, Yi Yu, Wei Cao, Guojian Fang, Yi Wan, Yue Gu, Yuepeng Wang, Yigang Li, Ying Yu, Qunshan Wang Source Type: research

Metabolic reprogramming orchestrates CD4+ T-cell immunological status and restores cardiac dysfunction in autoimmune induced-dilated cardiomyopathy mice
Cellular autoimmune responses, especially those mediated by T-cells, play vital roles in the immunopathogenesis of dilated cardiomyopathy (DCM). Metabolic reprogramming directly controls T-cell function, imprinting distinct functional fates. However, its contribution to T-cell dysfunction and the immunopathogenesis of DCM is unknown. Here, we found that in DCM patients, CD4+ T-cells exhibited immune dysfunction and glycolytic metabolic reprogramming based on extracellular acidification and oxygen consumption rates. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - August 6, 2019 Category: Cytology Authors: Jian Wu, Ping Sun, Qi Chen, Yong Sun, Ming Shi, Ge Mang, Shan Yu, Yang Zheng, Zhaoying Li, Meng Sun, Shaohong Fang, Yongxiang Zhang, Jinwei Tian, E. Mingyan, Maomao Zhang, Bo Yu Source Type: research

Narciclasine inhibits angiogenic processes by activation of Rho kinase and by downregulation of the VEGF receptor 2
The process of angiogenesis is involved in several pathological conditions, such as tumor growth or age-related macular degeneration. Although the available anti-angiogenic drugs have improved the therapy of these diseases, major drawbacks, such as unwanted side effects and resistances, still exist. Consequently, the search for new anti-angiogenic substances is still ongoing. Narciclasine, a plant alkaloid from different members of the Amaryllidaceae family, has extensively been characterized as anti-tumor compound. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - August 2, 2019 Category: Cytology Authors: Jacqueline Br äutigam, Iris Bischoff, Christoph Schürmann, Giulia Buchmann, Jeremy Epah, Simone Fuchs, Elke Heiss, Ralf P. Brandes, Robert Fürst Source Type: research

Cardioprotection of ( ±)-sodium 5-bromo-2-(α-hydroxypentyl) benzoate (BZP) on mouse myocardium I/R injury through inhibiting 12/15-LOX-2 activity
( ±)-Sodium5-bromo-2-(α-hydroxypentyl) benzoate (brand name: brozopine, BZP, 1a), derived from 1 to 3-n-Butylphthalide (NBP), has been reported to protect the brain from stoke and has been approved by CFDA in Phase I-II clinical trials. However, it remains to be investigated whether 1a may exhibit a ny cardioprotective effect on ischemia-reperfusion (I/R) injury. In the current study, C57BL/6 and ICR mice were pretreated with 1a, and myocardium I/R were then performed. We found that 1a not only significantly reduced the infarct size and improved cardiac contractile function after acute MI/R in both species, bu...
Source: Journal of Molecular and Cellular Cardiology - July 27, 2019 Category: Cytology Authors: Yue Xiao, Chuanjun Song, Qiao Lin, Xiaojing Shi, Wenquan Yu, Xin Huang, Huimin Wang, Yang Chen, Ruiyong Wang, Xuepeng Geng, Mingyang Qin, Kaizhao Hu, Yilin Fan, Yan Qiao, Erhe Gao, Wen Zhao, Junbiao Chang Source Type: research

Wnt1 inhibits vascular smooth muscle cell calcification by promoting ANKH expression
Wnt signaling plays a critical role in vascular calcification (VC). Wnt factors induce different physiological and pathological effects on cardiovascular functions. Wnt1, a ligand of Wnt/ β-catenin signaling, promotes pro-angiogenesis and reduces myocardial infarction. The role of Wnt1 on VC in chronic kidney disease (CKD) is not fully understood. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - July 27, 2019 Category: Cytology Authors: Beidong Chen, Yang Zhao, Duanyang Han, Ban Zhao, Yonghui Mao, Zhong-Kai Cui, Yun-Chin Chu, Lu Feng, Sen Yin, Cun-Yu Wang, Xian Wang, Ming-Jiang Xu, Gexin Zhao Source Type: research

Potent hERG channel inhibition by sarizotan, an investigative treatment for Rett Syndrome
This study investigated whether sarizotan inhibits hERG potassium channels and prolongs ventricular repolarization. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - July 27, 2019 Category: Cytology Authors: Hongwei Cheng, Chunyun Du, Yihong Zhang, Andrew F. James, Christopher E. Dempsey, Ana P. Abdala, Jules C. Hancox Source Type: research

Dynamic palmitoylation regulates trafficking of K channel interacting protein 2 (KChIP2) across multiple subcellular compartments in cardiac myocytes
K channel interacting protein 2 (KChIP2), initially cloned as Kv4 channel modulator, is a multi-tasking protein. In addition to modulating several cardiac ion channels at the plasma membrane, it can also modulate microRNA transcription inside nuclei, and interact with presenilins to modulate Ca release through RyR2 in the cytoplasm. However, the mechanism regulating its subcellular distribution is not clear. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - July 27, 2019 Category: Cytology Authors: Akshay Murthy, Samuel Workman, Min Jiang, Junping Hu, Ismat Sifa, Tytus Bernas, Wanchun Tang, Isabelle Deschenes, Gea-Ny Tseng Source Type: research

Exosomal CagA derived from Helicobacter pylori-infected gastric epithelial cells induces macrophage foam cell formation and promotes atherosclerosis
These results may provide new insights into the role of exosomal CagA in the pathogenesis of CagA-positive H. pylori infection-related atherosclerosis. It is suggested that preventing and eradicating CagA-positive H. pylori infection could reduce the incidence of atherosclerosis and related events. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - July 25, 2019 Category: Cytology Authors: Shuai Yang, Yuan-peng Xia, Xue-ying Luo, Shao-li Chen, Bo-wei Li, Zi-ming Ye, Sheng-cai Chen, Ling Mao, Hui-juan Jin, Ya-nan Li, Bo Hu Source Type: research

Patterns of ascending aortic dilatation and predictors of surgical replacement of the aorta: A comparison of bicuspid and tricuspid aortic valve patients over eight years of follow-up
Predictors of thoracic aorta growth and early cardiac surgery in patients with bicuspid aortic valve are undefined. Our aim was to identify predictors of ascending aorta dilatation and cardiac surgery in patients with bicuspid aortic valve (BAV). (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - July 23, 2019 Category: Cytology Authors: Valentina Agnese, Salvatore Pasta, Hector I. Michelena, Chiara Min à, Giuseppe Romano, Scipione Carerj, Concetta Zito, Joseph F. Maalouf, Thomas A. Foley, Giuseppe Raffa, Francesco Clemenza, Michele Pilato, Diego Bellavia Source Type: research

Roles and mechanisms of SUMOylation on key proteins in myocardial ischemia/reperfusion injury
Myocardial ischemia/reperfusion (MI/R) injury has a great influence on the prognosis of patients with acute coronary occlusion. The underlying mechanisms of MI/R injury are complex. While the incidence of MI/R injury is increasing every year, the existing therapies are not satisfactory. Recently, small ubiquitin-related modifier (SUMO), which is a post-translational modification and involved in many cell processes, was found to play remarkable roles in MI/R injury. Several proteins that can be SUMOylated were found to interfere with different mechanisms of MI/R injury. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - July 22, 2019 Category: Cytology Authors: Jingwen Chen, Yuanyuan Luo, Shuai Wang, Hong Zhu, Dongye Li Tags: Review article Source Type: research

Remodeling of substrate consumption in the murine sTAC model of heart failure
Energy metabolism and substrate selection are key aspects of correct myocardial mechanical function. Myocardial preference for oxidizable substrates changes in both hypertrophy and in overt failure. Previous work has shown that glucose oxidation is upregulated in overpressure hypertrophy, but its fate in overt failure is less clear. Anaplerotic flux of pyruvate into the tricarboxylic acid cycle (TCA) has been posited as a secondary fate of glycolysis, aside from pyruvate oxidation or lactate production. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - July 21, 2019 Category: Cytology Authors: Aslan Turer, Francisco Altamirano, Gabriele G. Schiattarella, Herman May, Thomas G. Gillette, Craig Malloy, Matthew E. Merritt Source Type: research

MicroRNA-1976 regulates degeneration of the sinoatrial node by targeting Cav1.2 and Cav1.3 ion channels
Sick sinus syndrome (SSS) is primarily a disease of the elderly, and age-dependent decrease in Cav1.2 and Cav1.3 Ca2+ channels within the sinus node has been shown to play an important role in sinoatrial node (SAN) degeneration; however, posttranscriptional mechanisms regulating decrease in Cav1.2 and Cav1.3 Ca2+ channels remain unclear. Some studies have reported that microRNAs (miRNAs) are involved in age-related cardiovascular diseases. Nevertheless, little is known about the roles of miRNAs in age-related SSS. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - July 11, 2019 Category: Cytology Authors: Jin Zhang, Feiyu Wei, Liqun Ding, Lilin Wang, Xi Zhang, Lin Yu, Rui Liu, Xiaohui Kuang, Baowei Jiao, Bin Yang, Jie Fan Source Type: research

Heat shock protein inducer GGA*-59 reverses contractile and structural remodeling via restoration of the microtubule network in experimental Atrial Fibrillation
Atrial Fibrillation (AF) is the most common progressive tachyarrhythmia. AF progression is driven by abnormalities in electrical impulse formation and contractile function due to structural remodeling of cardiac tissue. Previous reports indicate that structural remodeling is rooted in derailment of protein homeostasis (proteostasis). Heat shock proteins (HSPs) play a critical role in facilitating proteostasis. Hence, the HSP-inducing compound geranylgeranylacetone (GGA) and its derivatives protect against proteostasis derailment in experimental models for AF. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - July 11, 2019 Category: Cytology Authors: Xu Hu, Jin Li, Denise M.S. van Marion, Deli Zhang, Bianca J.J.M. Brundel Source Type: research

The lymphocyte adapter protein: A negative regulator of myocardial ischemia/reperfusion injury
In this study, increased expression of Lnk was detected upon the development of myocardial I/R injury. Mice were genetically engineered to investigate the role of Lnk in this pathological process. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - July 10, 2019 Category: Cytology Authors: Huang Jia, Yuning Sun, Lijuan Chen, Genshan Ma Source Type: research

Smooth muscle-specific TMEM16A expression protects against angiotensin II-induced cerebrovascular remodeling via suppressing extracellular matrix deposition
Cerebrovascular remodeling is the leading factor for stroke and characterized by increased extracellular matrix deposition, migration and proliferation of vascular smooth muscle cells, and inhibition of their apoptosis. TMEM16A is an important component of Ca2+-activated Cl − channels. Previously, we showed that downregulation of TMEM16A in the basilar artery was negatively correlated with cerebrovascular remodeling during hypertension. However, it is unclear whether TMEM16A participates in angiotensin II (Ang II)-induced vascular remodeling in mice that have TMEM16A gene modification. (Source: Journal of Molecular a...
Source: Journal of Molecular and Cellular Cardiology - July 10, 2019 Category: Cytology Authors: Xue-Lin Zeng, Lu Sun, Hua-Qing Zheng, Guan-Lei Wang, Yan-Hua Du, Xiao-Fei Lv, Ming-Ming Ma, Yong-Yuan Guan Source Type: research

Pseudo-bipolar spindle formation and cell division in postnatal binucleated cardiomyocytes
The majority of adult human, mouse and rat cardiomyocytes is not diploid mononucleated. Nevertheless, the current literature on heart regeneration based on cardiomyocyte proliferation focuses mainly on the proliferation capacity of diploid mononucleated cardiomyocytes, instead of the more abundant mononucleated polyploid or binucleated cardiomyocytes. Here, we aimed at a better understanding of the process of mitosis and cell division in postnatal binucleated cardiomyocytes. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - July 10, 2019 Category: Cytology Authors: Marina Leone, Felix B. Engel Tags: Short communication Source Type: research

Gut microbe-derived metabolite trimethylamine N-oxide accelerates fibroblast-myofibroblast differentiation and induces cardiac fibrosis
Trimethylamine N-oxide (TMAO), a gut microbe-derived metabolite of dietary choline and other trimethylamine-containing nutrients, has been associated with poor prognosis in coronary heart disease. However, the role and underlying mechanisms of TMAO in the cardiac fibrosis after myocardial infarction (MI) remains unclear. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - July 9, 2019 Category: Cytology Authors: Wenlong Yang, Shuning Zhang, Jianbing Zhu, Hao Jiang, Daile Jia, Tiantong Ou, Zhiyong Qi, Yunzeng Zou, Juying Qian, Aijun Sun, Junbo Ge Source Type: research

MicroRNAs fingerprint of bicuspid aortic valve
Aortic valve tissue is largely exposed to high blood flow. Cells belonging to aortic valve tissues are able to detect and respond to flow conditions changes. Bicuspid aortic valve (BAV) presents altered morphology, with only two abnormal cusps instead of three. This results in an alteration of blood flow dynamics on valve cusps and aortic wall, which may, in turn, increase the risk to develop aortic stenosis and/or regurgitation, endocarditis, aortopathy and/or aortic dissection.MicroRNAs (miRNAs) are short RNA strands regulating gene expression mainly through the inhibition of their target mRNAs. (Source: Journal of Molec...
Source: Journal of Molecular and Cellular Cardiology - July 3, 2019 Category: Cytology Authors: Sabatino Jolanda, Wicik Zofia, DeRosa Salvatore, Eyileten Ceren, Jakubik Daniel, Carmen Spaccarotella, Annalisa Mongiardo, Postula Marek, Indolfi Ciro Source Type: research

The metalloproteinase ADAM15 is upregulated by shear stress and promotes survival of endothelial cells
Reduced shear stress resulting from disturbed blood flow can impair endothelial integrity and drive the development of vascular inflammatory lesions. Metalloproteinases of the ADAM family have been implicated in the regulation of cell survival and inflammatory responses. Here we investigate the mechanism and function of ADAM15 upregulation in primary flow cultured endothelial cells.Transcriptomic analysis indicated that within the ADAM family ADAM15 mRNA is most prominently upregulated (4-fold) when endothelial cells are exposed to physiologic shear stress. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - July 1, 2019 Category: Cytology Authors: Aaron Babendreyer, Lisa Molls, Indra M. Simons, Daniela Dreymueller, Kristina Biller, Holger Jahr, Bernd Denecke, Reinier A. Boon, Sebastian Bette, Uwe Schnakenberg, Andreas Ludwig Source Type: research

Intracardiac administration of neutrophil protease cathepsin G activates noncanonical inflammasome pathway and promotes inflammation and pathological remodeling in non-injured heart
This study investigated whether neutrophil-derived cathepsin G (Cat.G) influences inflammation and remodeling in the absence of prior cardiac injury and cell death. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - June 25, 2019 Category: Cytology Authors: Sonni-Ali Miller, Mikhail A. Kolpakov, Xinji Guo, Binbin Du, Yen Nguyen, Tao Wang, Pamela Powel, Louis J. Dell'Italia, Abdelkarim Sabri Source Type: research

Inhibition of Rho-associated kinases suppresses cardiac myofibroblast function in engineered connective and heart muscle tissues
Cardiac fibrosis is a hallmark of heart failure for which there is no effective pharmacological therapy. By genetic modification and in vivo inhibitor approaches it was suggested that the Rho-associated kinases (ROCK1 and ROCK2) are involved in pro-fibrotic signalling in cardiac fibroblasts and that they may serve as targets for anti-fibrotic therapies. We demonstrate that simultaneous inhibition of ROCK1 and ROCK2 strongly interfered with tissue formation and their biomechanical properties in a model of engineered connective tissue (ECT), comprised of cardiac fibroblasts and collagen. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - June 21, 2019 Category: Cytology Authors: Gabriela Santos, Svenja Hartmann, Wolfram-Hubertus Zimmermann, Anne Ridley, Susanne Lutz Source Type: research

MIR148A family regulates cardiomyocyte differentiation of human embryonic stem cells by inhibiting the DLL1-mediated NOTCH signaling pathway
MicroRNAs (miRNAs), as a class of naturally occurring RNAs, play important roles in cardiac physiology and pathology. There are many miRNAs that show multifarious expression patterns during cardiomyocyte genesis. Here, we focused on the MIR148A family, which is composed of MIR148A, MIR148B and MIR152, and shares the same seed sequences. The expression levels of all MIR148A family members progressively increased during the differentiation of human embryonic stem cells (hESCs) into cardiomyocytes. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - June 21, 2019 Category: Cytology Authors: Xing Fang, Shumei Miao, You Yu, Fengyue Ding, Xinglong Han, Hongchun Wu, Zhen-Ao Zhao, Yongming Wang, Shijun Hu, Wei Lei Source Type: research

Phenotyping an adult zebrafish lamp2 cardiomyopathy model identifies mTOR inhibition as a candidate therapy
Adult zebrafish is an emerging vertebrate model for studying genetic basis of cardiomyopathies; but whether the simple fish heart can model essential features of hypertrophic cardiomyopathy (HCM) remained unknown. Here, we report a comprehensive phenotyping of a lamp2 knockout (KO) mutant. LAMP2 encodes a lysosomal protein and is a causative gene of Danon disease that is characterized by HCM and massive autophagic vacuoles accumulation in the tissues. There is no effective therapy yet to treat this most lethal cardiomyopathy in the young. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - June 19, 2019 Category: Cytology Authors: Alexey V. Dvornikov, Mingmin Wang, Jingchun Yang, Ping Zhu, Tai Le, Xueying Lin, Hung Cao, Xiaolei Xu Source Type: research

Sodium nitrite improves hypertension-induced myocardial dysfunction by mechanisms involving cardiac S-nitrosylation
Although nitrite improves vascular function and lowers blood pressure, its cardiac effects are not completely known. We investigated whether nitrite improves the cardiac function in normotensive and in hypertensive rats. Two-kidney, one-clip hypertension model (2K1C) was induced in Wistar rats. Blood pressure was evaluated by tail-cuff plethysmography over 6  weeks. By the end of week 2, hypertensive and normotensive rats received nitrite (daily dose of 1 or 15 mg/kg) by gavage for 4 weeks. Cardiac morphology and function were performed by transthoracic echocardiography. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - June 18, 2019 Category: Cytology Authors: Evandro M. Neto-Neves, Lucas C. Pinheiro, Renato C. Nogueira, Rafael L. Portella, Rose I. Batista, Jose E. Tanus-Santos Source Type: research

Corrigendum to “Correlational study on altered epicardial adipose tissue as a stratification risk factor for valve disease progression through IL-13 signaling” [Journal of Molecular and Cellular Cardiology 132 (2019) 210–2018]
The authors regret for the misunderstanding in author names during proof correction. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - June 18, 2019 Category: Cytology Authors: Elena Vianello, Massimiliano Maria Marrocco-Trischitta, Elena Dozio, Francesco Bandera, Lorenza Tacchini, Elena Canciani, Claudia Dellavia, Gerd Schmitz, Lorenzo Menicanti, Massimiliano Marco Corsi Romanelli Tags: Corrigendum Source Type: research

LncRNA PCFL promotes cardiac fibrosis via miR-378/GRB2 pathway following myocardial infarction
Long noncoding RNAs (lncRNAs) are a class of novel molecular regulators in cardiac development and diseases. However, the role of specific lncRNAs in cardiac fibrosis remains to be fully explored. The aim of the present study was to investigate the effects and underlying mechanisms of lncRNA PCFL (pro-cardiac fibrotic lncRNA) on cardiac fibrosis after myocardial infarction (MI).Cardiac fibroblasts (CFs) with gain and loss of function of PCFL and mice with global knockout or overexpression of PCFL were used to explore the effects of PCFL on cardiac fibrosis. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - June 17, 2019 Category: Cytology Authors: Fei Sun, Yuting Zhuang, Haixia Zhu, Hao Wu, Danyang Li, Linfeng Zhan, Wanqi Yang, Yin Yuan, Yilin Xie, Shuang Yang, Shenjian Luo, Wenmei Jiang, Jifan Zhang, Zhenwei Pan, Yanjie Lu Source Type: research

Alteration in ventricular pressure stimulates cardiac repair and remodeling
The mammalian heart undergoes complex structural and functional remodeling to compensate for stresses such as pressure overload. While studies suggest that, at best, the adult mammalian heart is capable of very limited regeneration arising from the proliferation of existing cardiomyocytes, how myocardial stress affects endogenous cardiac regeneration or repair is unknown. To define the relationship between left ventricular afterload and cardiac repair, we induced left ventricle pressure overload in adult mice by constriction of the ascending aorta (AAC). (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - June 17, 2019 Category: Cytology Authors: Kazumasa Unno, Angelos Oikonomopoulos, Yusuke Fujikawa, Yusuke Okuno, Singo Narita, Tomohiro Kato, Ryo Hayashida, Kazuhisa Kondo, Rei Shibata, Toyoaki Murohara, Yanfei Yang, Seema Dangwal, Konstantina-Ioanna Sereti, Qiu Yiling, Kory Johnson, Alokkumar Jha Source Type: research

Epigenetics and vascular diseases
Cardiovascular disease remains the number one cause of death and disability worldwide despite significant improvements in diagnosis, prevention, and early intervention efforts. There is an urgent need for improved understanding of cardiovascular processes responsible for disease development in order to develop more effective therapeutic strategies. Recent knowledge gleaned from the study of epigenetic mechanisms in the vasculature has uncovered new potential targets for intervention. Herein, we provide an overview of epigenetic mechanism, and review recent findings related to epigenetics in vascular diseases, highlighting ...
Source: Journal of Molecular and Cellular Cardiology - June 15, 2019 Category: Cytology Authors: Matthew S. Stratton, Floriana Maria Farina, Leonardo Elia Source Type: research

Impaired SIRT3 activity mediates cardiac dysfunction in endotoxemia by calpain-dependent disruption of ATP synthesis
Sepsis-induced cardiomyopathy contributes to the high mortality of septic shock in critically ill patients. Since the underlying mechanisms are incompletely understood, we hypothesized that sepsis-induced impairment of sirtuin 3 (SIRT3) activity contributes to the development of septic cardiomyopathy. (Source: Journal of Molecular and Cellular Cardiology)
Source: Journal of Molecular and Cellular Cardiology - June 12, 2019 Category: Cytology Authors: Christoph Koentges, Mar ía C. Cimolai, Katharina Pfeil, Dennis Wolf, Timoteo O. Marchini, Aleksandre Tarkhnishvili, Michael M. Hoffmann, Katja E. Odening, Philipp Diehl, Constantin von zur Mühlen, Silvia Alvarez, Christoph Bode, Andreas Zirlik, Heiko Bu Source Type: research

Mechanism of 17 β-estradiol stimulated integration of human mesenchymal stem cells in heart tissue
Scarcity of gender specific donor hearts highlights the importance of mesenchymal stem cells (MSCs) as a therapeutic tool for heart repair. However, inefficient incorporation, retention, and activity of MSCs in cardiac tissue remain an obstacle. Since surges in follicular estradiol (E2; μmolar-range) trigger tissue remodeling (e.g. ovulation) and E2 exerts beneficial actions on the cardiovascular system, we hypothesized that E2 may promote/improve MSC-mediated cardiac repair processes. Using Wharton's jelly (WJ)-derived MSCs we assessed the effects of E2 on MSC proliferation, dire cted migration, and engraftment in muri...
Source: Journal of Molecular and Cellular Cardiology - June 12, 2019 Category: Cytology Authors: Maria Cristina Mihai, Mirel Adrian Popa, Viorel Iulian Suica, Felicia Antohe, Edwin K. Jackson, Maya Simionescu, Raghvendra K. Dubey Source Type: research

Light on the molecular and cellular mechanisms of bicuspid aortic valve to unveil phenotypic heterogeneity
Research on bicuspid aortic valve disease (BAV) and related complications has grown in an exponential manner in the last decades. However, the current knowledge of the mechanisms underlying the development of this disease is still limited, since all clinical and surgical studies on BAV mainly focused their objects on its major vascular complications, such as ascending aortic aneurysms and dissection. It is now clear that a better understanding of the pivotal molecular and cellular pathophysiological aspects of bicuspid valve aortopathy, including natural history, phenotypic expression, histology, cellular mechanisms and pa...
Source: Journal of Molecular and Cellular Cardiology - June 11, 2019 Category: Cytology Authors: Carmela Rita Balistreri, Elena Cavarretta, Sebastiano Sciarretta, with the special contribution with Prof Giacomo Frati Source Type: research