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Nutrition: Vitamin C

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Total 28 results found since Jan 2013.

Vitamin C Prevents Hydrocortisone-Induced Injury in HMEC-1 through Promoting Bestrophin-3 Expression.
CONCLUSIONS: VC can efficiently attenuate HC-induced HMEC-1 cell injury, which may be related to promote Best-3 expression. PMID: 30672332 [PubMed - as supplied by publisher]
Source: Nutrition and Cancer - January 23, 2019 Category: Cancer & Oncology Authors: Wang X, Zhang G, Zhu C, Lin L, Zhao Z, Yu X, Liu G, Zhang H, Li Q, Dong W, Wang J Tags: Nutr Cancer Source Type: research

TXNIP-mediated nuclear factor- κB signaling pathway and intracellular shifting of TXNIP in uric acid-induced NLRP3 inflammasome.
CONCLUSION: This study showed that activation of MSU-induced NLRP3 inflammasome requires TXNIP-mediated NF-κB signaling pathway and intracellular TXNIP shifting. PMID: 30833078 [PubMed - as supplied by publisher]
Source: Biochemical and Biophysical Research communications - February 28, 2019 Category: Biochemistry Authors: Kim SK, Choe JY, Park KY Tags: Biochem Biophys Res Commun Source Type: research

Glyoxalate reductase/hydroxypyruvate reductase interacts with the sodium-dependent vitamin C transporter-1 to regulate cellular vitamin C homeostasis.
Abstract The human sodium-dependent vitamin C transporter 1 (hSVCT1) contributes to cellular uptake of ascorbic acid (AA). While different aspects of hSVCT1 cell biology have been extensively studied, nothing is currently known about the broader hSVCT1 interactome that modulates its role in cellular physiology. Here, we identify the enzyme human glyoxalate reductase/hydroxypyruvate reductase (hGR/HPR) as an hSVCT1 associated protein by yeast two-hybrid (Y2H) screening of a human liver cDNA library. The interaction between hSVCT1 and hGR/HPR was further confirmed by in vitro GST-pull-down assay, in vivo co-immunopr...
Source: American Journal of Physiology. Gastrointestinal and Liver Physiology - April 18, 2013 Category: Physiology Authors: Subramanian VS, Nabokina SM, Patton JR, Marchant JS, Moradi H, Said HM Tags: Am J Physiol Gastrointest Liver Physiol Source Type: research

Glyoxalate reductase/hydroxypyruvate reductase interacts with the sodium-dependent vitamin C transporter-1 to regulate cellular vitamin C homeostasis
The human sodium-dependent vitamin C transporter 1 (hSVCT1) contributes to cellular uptake of ascorbic acid (AA). Although different aspects of hSVCT1 cell biology have been extensively studied, nothing is currently known about the broader hSVCT1 interactome that modulates its role in cellular physiology. Here, we identify the enzyme human glyoxalate reductase/hydroxypyruvate reductase (hGR/HPR) as an hSVCT1 associated protein by yeast two-hybrid (Y2H) screening of a human liver cDNA library. The interaction between hSVCT1 and hGR/HPR was further confirmed by in vitro GST pull-down assay, in vivo coimmunoprecipitation and ...
Source: AJP: Gastrointestinal and Liver Physiology - June 15, 2013 Category: Gastroenterology Authors: Subramanian, V. S., Nabokina, S. M., Patton, J. R., Marchant, J. S., Moradi, H., Said, H. M. Tags: LIVER AND BILIARY TRACT Source Type: research

Mitochondrial ascorbic acid transport is mediated by a low-affinity form of the sodium-coupled ascorbic acid transporter-2 (SVCT2).
We report here that human HEK-293 cells express a mitochondrial low-affinity ascorbic acid transporter that molecularly corresponds to SVCT2, a member of the sodium-coupled ascorbic acid transporter family 2. The transporter SVCT1 is absent from the HEK-293 cells. Confocal colocalization experiments with anti-SVCT2 and anti-organelle protein markers revealed that most of the SVCT2 immunoreactivity was associated with mitochondria, with minor colocalization at the endoplasmic reticulum and very low immunoreactivity at the plasma membrane. Immunoblotting of proteins extracted from highly purified mitochondrial fractions conf...
Source: Free Radical Biology and Medicine - March 1, 2014 Category: Biology Authors: Muñoz-Montesino C, Roa FJ, Peña E, González M, Sotomayor K, Inostrosa E, Muñoz C, González I, Maldonado M, Soliz C, Reyes AM, Carlos Vera J, Rivas CI Tags: Free Radic Biol Med Source Type: research

Ascorbic acid reduces HMGB1 secretion in lipopolysaccharide-activated RAW 264.7 cells and improves survival rate in septic mice by activation of Nrf2/HO-1 signals.
Abstract High mobility group box 1 (HMGB1) is now recognized as a late mediator of sepsis. We tested hypothesis that ascorbic acid (AscA) induces heme oxygenase (HO)-1 which inhibits HMGB1 release in lipopolysaccharide (LPS)-stimulated cells and increases survival of septic mice. AscA increased HO-1 protein expression in a concentration- and time-dependent manner via Nrf2 activation in RAW 264.7 cells. HO-1 induction by AscA was significantly reduced by Nrf2 siRNA-transfected cells. Mutation of cysteine to serine of keap-1 proteins (C151S, C273S, and C288S) lost the ability of HO-1 induction by AscA, due to failur...
Source: Biochemical Pharmacology - April 17, 2015 Category: Drugs & Pharmacology Authors: Kim SR, Ha YM, Kim YM, Park EJ, Kim JW, Park SW, Kim HJ, Chung HT, Chang KC Tags: Biochem Pharmacol Source Type: research

Ascorbic acid and ascorbate-2-phosphate decrease HIF activity and malignant properties of human melanoma cells
Background: Hypoxia inducible factor-1 alpha (HIF-1α) is thought to play a role in melanoma carcinogenesis. Posttranslational regulation of HIF-1α is dependent on Prolyl hydroxylase (PHD 1–3) and Factor Inhibiting HIF (FIH) hydroxylase enzymes, which require ascorbic acid as a co-factor for optimal function. Depleted intra-tumoral ascorbic acid may thus play a role in the loss of HIF-1α regulation in melanoma. These studies assess the ability of ascorbic acid to reduce HIF-1α protein and transcriptional activity in metastatic melanoma and reduce its invasive potential. Methods: HIF-1α protein was evaluated by wester...
Source: BMC Cancer - November 7, 2015 Category: Cancer & Oncology Authors: Sarah MilesAdam FischerSandeep JoshiRichard Niles Source Type: research

Combined treatment with vitamin C and sulindac synergistically induces p53- and ROS-dependent apoptosis in human colon cancer cells.
In this study, we evaluated a combinatorial strategy utilizing sulindac and vitamin C. The death of HCT116 cells upon combination therapy occurred via a p53-mediated mechanism. The combination therapeutic resistance developed in isogenic p53 null HCT116 cells and siRNA-mediated p53 knockdown HCT116 cells, but the exogenous expression of p53 in p53 null isogenic cells resulted in the induction of cell death. In addition, we investigated an increased level of intracellular ROS (reactive oxygen species), which was preceded by p53 activation. The expression level of PUMA (p53-upregulated modulator of apoptosis), but not Bim, w...
Source: Toxicology Letters - June 19, 2016 Category: Toxicology Authors: Gong EY, Shin YJ, Hwang IY, Kim JH, Kim SM, Moon JH, Shin JS, Lee DH, Hur DY, Jin DH, Hong SW, Lee WK, Lee WJ Tags: Toxicol Lett Source Type: research

Effects of Ascorbyl-2-phosphate Magnesium on Human Keratinocyte Toxicity and Pathological Changes by Sorafenib.
Abstract Hand-foot skin reaction is recognized as one of the most common adverse events related to multiple tyrosine kinase inhibitors, but an effective prevention method has not been identified. The chief aim of this study was to find a mechanism-based preventive method for the skin toxicity induced by sorafenib using vitamin C derivatives. The effects of ascorbyl-2-phosphate magnesium (P-VC-Mg) on the molecular and pathological changes induced by sorafenib were investigated in human keratinocyte HaCaT cells. The cell growth inhibition and apoptotic effects of sorafenib were attenuated by P-VC-Mg. Moreover, P-VC-...
Source: Biological and Pharmaceutical Bulletin - September 6, 2017 Category: Drugs & Pharmacology Authors: Yamamoto K, Shichiri H, Ishida T, Kaku K, Nishioka T, Kume M, Makimoto H, Nakagawa T, Hirano T, Bito T, Nishigori C, Yano I, Hirai M Tags: Biol Pharm Bull Source Type: research

Vitamin C Attenuates Sodium Fluoride-Induced Mitochondrial Oxidative Stress and Apoptosis via Sirt1-SOD2 Pathway in F9 Cells.
Abstract Increasing evidence has suggested an important role played by reactive oxygen species (ROS) in the pathogenesis of fluorosis. Accumulating evidence demonstrates that vitamin C administration ameliorate sodium fluoride (NaF)-induced oxidative stress. However, the potentially beneficial effects of vitamin C against NaF-induced cytotoxicity and the underlying molecular mechanisms of this protection are not fully understood. Here, we found that NaF stimulated cytotoxicity, increased mitochondrial reactive oxygen species (mROS) production, and induced apoptosis in F9 embryonic carcinoma cells. Consistent with ...
Source: Biological Trace Element Research - December 18, 2018 Category: Biology Authors: Peng W, Xu S, Zhang J, Zhang Y Tags: Biol Trace Elem Res Source Type: research

Soluble klotho regulates bone differentiation by upregulating expression of the transcription factor EGR ‐1
AbstractKlotho is a transmembrane protein known to regulate aging and lifespan. Soluble klotho (sKl), a truncated form of klotho, regulates various cell signaling pathways, including bone development. Here, we investigated the relationship between sKl and the zinc ‐finger transcription factor early growth response protein 1 (EGR‐1) on bone formation. We find that sKl induces the expression of EGR‐1 mRNA and protein. Through mutational analysis, we identify the 130 bp region on the EGR‐1 promoter that is responsive to sKl overexpression. Additionally, sKl induces the expression of markers of bone differentiation (BM...
Source: FEBS Letters - September 18, 2019 Category: Biochemistry Authors: Nguyen Khanh Toan, Nguyen Chi Tai, Soo ‐A Kim, Sang‐Gun Ahn Tags: RESEARCH ARTICLES Source Type: research

TET-mediated DNA demethylation plays an important role in arsenic-induced HBE cells oxidative stress via regulating promoter methylation of OGG1 and GSTP1.
Abstract Environmental exposure to arsenic remains a worldwide public health challenge. Oxidative stress and aberrant DNA methylation are both characteristics of arsenic toxicology; however, the relationship between these is not well understood. Ten-eleven translocation (TET1, TET2 and TET3), which is the conversion of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), plays a central role in the DNA demethylation process. Further, it can prevent cytosine-phosphate-guanine (CpG) islands from developing abnormal hypermethylation under oxidative stress. Here, we observed that NaAsO2 could induce oxidative str...
Source: Toxicology in Vitro - December 31, 2020 Category: Toxicology Authors: Wang Q, Wang W, Zhang A Tags: Toxicol In Vitro Source Type: research

The role of the voltage-gated potassium channel, Kv2.1 in prostate cancer cell migration.
Abstract Voltage-gated potassium (Kv) channels are involved in many important cellular functions and play pivotal roles in cancer progression. The expression level of Kv2.1 was observed to be higher in the highly metastatic prostate cancer cells (PC-3), specifically in their membrane, than in immortalized prostate cells (WPMY-1 cells) and comparatively less metastatic prostate cancer cells (LNCaP and DU145 cells). However, Kv2.1 expression was significantly decreased when the cells were treated with anti-oxidants, such as N-acetylcysteine or ascorbic acid, implying that the highly expressed Kv2.1 could detect reac...
Source: BMB Reports - January 7, 2021 Category: Biochemistry Authors: Park HW, Song MS, Sim HJ, Ryu PD, Lee SY Tags: BMB Rep Source Type: research

The role of the voltage-gated potassium channel, Kv2.1 in prostate cancer cell migration
BMB Rep. 2021 Jan 7:5184. Online ahead of print.ABSTRACTVoltage-gated potassium (Kv) channels are involved in many important cellular functions and play pivotal roles in cancer progression. The expression level of Kv2.1 was observed to be higher in the highly metastatic prostate cancer cells (PC-3), specifically in their membrane, than in immortalized prostate cells (WPMY-1 cells) and comparatively less metastatic prostate cancer cells (LNCaP and DU145 cells). However, Kv2.1 expression was significantly decreased when the cells were treated with anti-oxidants, such as N-acetylcysteine or ascorbic acid, implying that the hi...
Source: BMB Reports - January 7, 2021 Category: Biochemistry Authors: Hyun Woo Park Min Seok Song Hun Ju Sim Pan Dong Ryu So Yeong Lee Source Type: research