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Total 19 results found since Jan 2013.

Cell-penetrating peptide-siRNA conjugate loaded YSA-modified nanobubbles for ultrasound triggered siRNA delivery.
In conclusion, the application of CPP-siRNA/YSA-NB with ultrasound may provide a strategy for the selective and efficient delivery of siRNA. PMID: 26492155 [PubMed - as supplied by publisher]
Source: Colloids and Surfaces - October 19, 2015 Category: Biotechnology Authors: Xie X, Yang Y, Lin W, Liu H, Liu H, Yang Y, Chen Y, Fu X, Deng J Tags: Colloids Surf B Biointerfaces Source Type: research

Thermal and magnetic dual-responsive liposomes with a cell-penetrating peptide-siRNA conjugate for enhanced and targeted cancer therapy.
In conclusion, the application of siRNA-CPPs/TML under an AC magnetic field represents a new strategy for the selective and efficient delivery of siRNA. PMID: 27429294 [PubMed - as supplied by publisher]
Source: Colloids and Surfaces - July 1, 2016 Category: Biotechnology Authors: Yang Y, Xie X, Xu X, Xia X, Wang H, Li L, Dong W, Ma P, Yang Y, Liu Y, Mei X Tags: Colloids Surf B Biointerfaces Source Type: research

Redox-triggered intracellular siRNA delivery.
Abstract Gene silencing using small interfering RNA (siRNA) is a promising strategy for the treatment of multiple diseases. However, the low in vivo stability of siRNA, its poor pharmacokinetics and inability to penetrate inside cells limit its employment in the clinic. Here, we present a novel redox-sensitive micellar nanopreparation based on a triple conjugate of polyethylene glycol, polyethyleneimine and phosphatidylethanolamine, PEG-SS-PEI-PE (PSSPD). This non-toxic system efficiently condenses siRNA and specifically downregulates target green fluorescent protein (GFP) only under reducing conditions via intrac...
Source: Chemical Communications - June 5, 2018 Category: Chemistry Authors: Mutlu Agardan NB, Sarisozen C, Torchilin VP Tags: Chem Commun (Camb) Source Type: research

SLN124, a Galnac-siRNA Conjugate Targeting TMPRSS6, for the Treatment of Iron Overload and Ineffective Erythropoiesis Such As in Beta-Thalassemia
Accumulation of excess iron in tissues causes organ damage and dysfunction and may lead to serious clinical consequences including liver cirrhosis, diabetes, growth retardation and heart failure. Iron overload is a major health threat in iron loading anemias, like beta-thalassemia, myelodysplastic syndrome and in hereditary hemochromatosis. In patients with beta-thalassemia major, iron overload develops due to frequent blood transfusions to control the severe anemia. In addition, iron overload also occurs in patients with beta thalassemia intermedia (non-transfusion dependent beta-thalassemia). In the later cases, iron ove...
Source: Blood - November 21, 2018 Category: Hematology Authors: Altamura, S., Altamura, S., Muckenthaler, M. U., Dames, S., Frauendorf, C., Schubert, S., Aleku, M., Vadolas, J., Grigoriadis, G., Zugel, U. Tags: 102. Regulation of Iron Metabolism: Poster II Source Type: research

High-Throughput Characterization of Viral and Cellular Protein Expression Patterns During JC Polyomavirus Infection
Discussion The study of viral infections in vitro has provided innumerable advances to the field of virology. However, the lack of rapid and efficient screening tools has hindered research progress for some viruses, like JCPyV (Houff et al., 1983; Zu Rhein, 1983; Assetta and Atwood, 2017). To overcome this challenge, the development of high-throughput analyses is needed to help aid in the production of large data sets and generation of multiple lines of inquiry. Current methodologies for analyzing JCPyV infectivity predominantly rely on manual quantitation of infection by indirect immunodetection of viral proteins by epif...
Source: Frontiers in Microbiology - April 16, 2019 Category: Microbiology Source Type: research

The knockdown efficiency of telomere associated genes with specific methodology in a zebrafish cell line
Biochimie. 2021 Jun 29:S0300-9084(21)00158-9. doi: 10.1016/j.biochi.2021.06.013. Online ahead of print.ABSTRACTZebrafish is broadly used as a model organism in gene loss-of-function studies in vivo, but its employment in vitro is greatly limited by the lack of efficient gene knockdown approaches in zebrafish cell lines such as ZF4. In this article, we attempt to induce silencing of telomere associated genes in ZF4 by applying the frequently-used siRNA transfection technology and a novel moiety-linked morpholino(vivo-MO). By proceeding with integrated optimization of siRNAs transfection and vivo-MOs treatment, we compared f...
Source: Biochimie - July 2, 2021 Category: Biochemistry Authors: Xuefei Hu Shuaiyun Gao Peng Wang Yulin Zhou Kehua Chen Qiaowen Chen Bo Wang Weiguo Hu Peng Cheng Rita Eid Marie-Jos èph Giraud-Panis Lei Wang Eric Gilson Jing Ye Yiming Lu Source Type: research

HMGB1-NLRP3-P2X7R pathway participates in PM < sub > 2.5 < /sub > -induced hippocampal neuron impairment by regulating microglia activation
In this study, we focused on the role of HMGB1-NLRP3-P2X7R pathway which mediated the microglia activation in hippocampal neurons impairment induced by PM2.5 using a co-culture model of microglia and hippocampal neurons. We found that PM2.5 resulted in activated microglia and HMGB1-NLRP3 inflammatory pathway, and elevated proinflammatory cytokines of IL-18 and IL-1β in a dose-dependent manner. Notably, we next utilized previously reported pharmacological inhibitors or siRNA for HMGB1 and found that they significantly inhibited the activation of downstream NLRP3 and MAPK pathways derived from PM2.5 exposure, and down-regul...
Source: Ecotoxicology and Environmental Safety - May 23, 2022 Category: Environmental Health Authors: Chong Liu Yingjie She Jia Huang Yongping Liu Wanwei Li Can Zhang Tianliang Zhang Li Yu Source Type: research

Differential regulation of HIF‐mediated pathways increases mitochondrial metabolism and ATP production in hypoxic osteoclasts.
Abstract Inappropriate osteoclast activity instigates pathological bone loss in rheumatoid arthritis. We have investigated how osteoclasts generate sufficient ATP for the energy‐intensive process of bone resorption in the hypoxic microenvironment associated with this rheumatic condition. We show that in human osteoclasts differentiated from CD14+ monocytes hypoxia (24h, 2% O2) (i) increases ATP production and mitochondrial electron transport chain activity (Alamar Blue, O2 consumption); (ii) increases glycolytic flux (glucose consumption, lactate production) and (iii) increases glutamine consumption. We demonstrate that ...
Source: The Journal of Pathology - January 1, 2013 Category: Pathology Authors: Karl J Morten, Luned Badder, Helen J Knowles Tags: Original Paper Source Type: research

Metastatic prostate cancer cell-specific phage-like particles as a targeted gene-delivery system
Conclusion: Successful employment of phage-like particles expressing emerald green fluorescent protein genes targeted to prostate cancer cells PC-3M confirms a prospect of their use for targeted delivery of therapeutic genes to cancer cells.
Source: Journal of Nanobiotechnology - September 23, 2013 Category: Nanotechnology Authors: Olusegun FagbohunRobert KazmierczakValery PetrenkoAbraham Eisenstark Source Type: research

miR-Synth: a computational resource for the design of multi-site multi-target synthetic miRNAs
RNAi is a powerful tool for the regulation of gene expression. It is widely and successfully employed in functional studies and is now emerging as a promising therapeutic approach. Several RNAi-based clinical trials suggest encouraging results in the treatment of a variety of diseases, including cancer. Here we present miR-Synth, a computational resource for the design of synthetic microRNAs able to target multiple genes in multiple sites. The proposed strategy constitutes a valid alternative to the use of siRNA, allowing the employment of a fewer number of molecules for the inhibition of multiple targets. This may represe...
Source: Nucleic Acids Research - May 19, 2014 Category: Research Authors: Lagana, A., Acunzo, M., Romano, G., Pulvirenti, A., Veneziano, D., Cascione, L., Giugno, R., Gasparini, P., Shasha, D., Ferro, A., Croce, C. M. Tags: Computational Biology Source Type: research

Inhibitory effect of ent-Sauchinone on amyloidogenesis via inhibition of STAT3-mediated NF-kappaB activation in cultured astrocytes and microglial BV-2 cells
Conclusions: These results indicated that ent-Sauchinone inhibited neuroinflammation and amyloidogenesis through the inhibition of STAT3-mediated NF-kappaB activity, and thus could be applied in the treatment of neuro-inflammatory diseases, including Alzheimer's disease.
Source: Journal of Neuroinflammation - July 2, 2014 Category: Neurology Authors: Suk-Young SongYu JungChul HwangHee LeeChang SokJoo KimSang LeeHyun SeoByung HyunDong ChoiSang HanYoung HamBang HwangJin Hong Source Type: research

Inhibitory effect of ent-Sauchinone on amyloidogenesis via inhibition of STAT3-mediated NF-¿B activation in cultured astrocytes and microglial BV-2 cells
Conclusions: These results indicated that ent-Sauchinone inhibited neuroinflammation and amyloidogenesis through the inhibition of STAT3-mediated NF-kappaB activity, and thus could be applied in the treatment of neuro-inflammatory diseases, including Alzheimer's disease.
Source: Journal of Neuroinflammation - July 2, 2014 Category: Neurology Authors: Suk-Young SongYu JungChul HwangHee LeeChang SokJoo KimSang LeeHyun SeoByung HyunDong ChoiSang HanYoung HamBang HwangJin Hong Source Type: research

Endothelial sphingosine kinase/SPNS2 axis is critical for vessel-like formation by human mesoangioblasts
In this study, we aimed to identify the role of S1P axis in the intercellular communication between human mesenchymal progenitor mesoangioblasts (MAB) and endothelial cells (human microvascular endothelial cells (H-MVEC)) in the formation of capillary-like structures. We demonstrated that the S1P biosynthetic pathway brought about by sphingosine kinases (SK) SK1 and SK2 as well as spinster homolog 2 (SPNS2) transporter in H-MVEC is crucial for MAB migration measured by Boyden chambers and for the formation and stabilization of capillary-like structures in a 3D Matrigel culture. Moreover, the conditioned medium (CM) harvest...
Source: Journal of Molecular Medicine - May 9, 2015 Category: Molecular Biology Source Type: research