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Infectious Disease: Dengue Fever

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Total 65 results found since Jan 2013.

Viruses, Vol. 11, Pages 335: Annexin II as a Dengue Virus Serotype 2 Interacting Protein Mediating Virus Interaction on Vero Cells
-Yee Chee Recent evidence has demonstrated that dengue virus requires active filopodia formation for a successful infection. However, the cellular factor involved in the interaction has not been fully elucidated. We used a combination of virus overlay protein binding assay and LC-MS/MS, and identified annexin II as a dengue virus serotype 2 (DENV2) interacting protein on Vero cells, upon filopodia induction. Flow cytometry analysis showed annexin II on the Vero cells surface increased when DENV2 was added. The amount of annexin II in the plasma membrane fraction was reduced as the infection progressed. Antibody-mediate...
Source: Viruses - April 8, 2019 Category: Virology Authors: Isah Abubakar Aliyu King-Hwa Ling Nur Fariesha Md Hashim Jia-Yong Lam Hui-Yee Chee Tags: Article Source Type: research

A Role for the Insulin Receptor in the Suppression of Dengue Virus and Zika Virus in Wolbachia-Infected Mosquito Cells
This study identifies Wolbachia-mediated downregulation of insulin receptor kinase activity as a mechanism contributing to the blocking of super-infection by arboviruses.Graphical Abstract
Source: Cell Reports - January 16, 2019 Category: Cytology Source Type: research

Dengue virus requires apoptosis linked gene-2-interacting protein X (ALIX) for viral propagation.
In this study the interaction between DENV NS3 and ALIX was investigated by co-immunoprecipitation, and functional significance assessed by investigation of DENV production in ALIX expression regulated cells. The results showed that ALIX both interacted and co-localized with DENV NS3 protein and that upregulation of ALIX resulted in a significantly increased viral titer, while either siRNA or CRISPR-Cas9 mediated down regulation of ALIX significantly reduced viral production, without affecting relative DENV genome levels. These results are consistent with ALIX playing a significant role in the DENV replication cycle either...
Source: Virus Research - December 29, 2018 Category: Virology Authors: Thepparit C, Khongwichit S, Ketsuwan K, Libsittikul S, Auewarakul P, Smith DR Tags: Virus Res Source Type: research

Inhibition of dengue virus infection by small interfering RNAs that target highly conserved sequences in the NS4B or NS5 coding regions
AbstractDengue fever is one of the most common viral infections in the world. Although a vaccine against dengue virus (DENV) has been approved in several countries, this disease is still considered a public health priority worldwide. The ability of three small interfering RNAs (FG-siRNAs) targeting conserved sequences in the NS4B and NS5 regions of the DENV genome to inhibit DENV replication was testedin vitro in both Vero and C6/36 cells. The FG-siRNAs were effective against DENV-1, -3, and -4, but not DENV-2. A fourth siRNA specifically targeting the NS5 region of the DENV-2 genome (SG-siRNA) was designed and tested agai...
Source: Archives of Virology - February 1, 2018 Category: Virology Source Type: research

The Host Protein Reticulon 3.1A Is Utilized by Flaviviruses to Facilitate Membrane Remodelling
In this study, we identified a key role for the membrane-bending host protein Reticulon 3.1 (RTN3.1A) during the replication cycle of three flaviviruses: West Nile virus (WNV), Dengue virus (DENV), and Zika virus (ZIKV). We observed that, during infection, RTN3.1A is redistributed and recruited to the viral replication complex, a recruitment facilitated via the WNV NS4A protein, however, not DENV or ZIKV NS4A. Critically, small interfering RNA (siRNA)-mediated knockdown of RTN3.1A expression attenuated WNV, DENV, and ZIKV replication and severely affected the stability and abundance of the NS4A protein, coinciding with a s...
Source: Cell Reports - November 7, 2017 Category: Cytology Source Type: research

Unique Requirement for ESCRT Factors in Flavivirus Particle Formation on the Endoplasmic Reticulum
Publication date: Available online 18 August 2016 Source:Cell Reports Author(s): Keisuke Tabata, Masaru Arimoto, Masashi Arakawa, Atsuki Nara, Kazunobu Saito, Hiroko Omori, Arisa Arai, Tomohiro Ishikawa, Eiji Konishi, Ryosuke Suzuki, Yoshiharu Matsuura, Eiji Morita Flavivirus infection induces endoplasmic reticulum (ER) membrane rearrangements to generate a compartment for replication of the viral genome and assembly of viral particles. Using quantitative mass spectrometry, we identified several ESCRT (endosomal sorting complex required for transport) proteins that are recruited to sites of virus replication on the ER. S...
Source: Cell Reports - August 18, 2016 Category: Cytology Source Type: research

The anti ‑dengue virus properties of statins may be associated with alterations in the cellular antiviral profile expression.
The anti‑dengue virus properties of statins may be associated with alterations in the cellular antiviral profile expression. Mol Med Rep. 2016 Jul 13; Authors: Bryan-Marrugo OL, Arellanos-Soto D, Rojas-Martinez A, Barrera-Saldaña H, Ramos-Jimenez J, Vidaltamayo R, Rivas-Estilla AM Abstract Dengue virus (DENV) susceptibility to cholesterol depleting treatments has been previously reported. There are numerous questions regarding how DENV seizes cellular machinery and cholesterol to improve viral production and the effect of cholesterol sequestering agents on the cellular antiviral response. The aim of...
Source: Molecular Medicine Reports - July 21, 2016 Category: Molecular Biology Tags: Mol Med Rep Source Type: research

siRNA-Mediated Silencing of doublesex during Female Development of the Dengue Vector Mosquito Aedes aegypti
by Keshava Mysore, Longhua Sun, Michael Tomchaney, Gwyneth Sullivan, Haley Adams, Andres S. Piscoya, David W. Severson, Zainulabeuddin Syed, Molly Duman-Scheel The development of sex-specific traits, including the female-specific ability to bite humans and vector disease, is critical for vector mosquito reproduction and pathogen transmission. Doublesex (Dsx), a terminal transcription factor in the sex determination pathway, is known to regulate sex-specific gene expression during development of the dengue fever vector mosquito Aedes aegypti. Here, the effects of developmental siRNA-mediated dsx silencing were assessed in ...
Source: PLoS Neglected Tropical Diseases - November 6, 2015 Category: Tropical Medicine Authors: Keshava Mysore et al. Source Type: research

Dengue virus requires the unsaturated fatty acid biosynthesis pathway for its infection in the mammalian host
Publication date: May–July 2015 Source:New Horizons in Translational Medicine, Volume 2, Issues 4–5 Author(s): Rebekah C. Gullberg, Richard J. Kuhn, Rushika Perera Dengue virus (DENV) infection is a significant global health concern with over 40% of the world’s population at risk and currently no therapeutics or vaccines available. Understanding host viral interactions is key to developing novel therapeutic options. Dengue virus is a positive sense RNA virus that induces the formation of invaginations in the endoplasmic reticulum to replicate its genome. Increased phospholipid biosynthesis is key to the formatio...
Source: New Horizons in Translational Medicine - September 13, 2015 Category: Research Source Type: research

Flavivirus sfRNA suppresses antiviral RNA interference in cultured cells and mosquitoes and directly interacts with the RNAi machinery.
In this study, we demonstrate that two flaviviruses, Dengue virus and Kunjin virus, significantly repress siRNA-mediated RNAi in infected human cells as well as during infection of the mosquito vector Culex quinquefasciatus. Arthropod-borne flaviviruses generate a small structured non-coding RNA from the viral 3' UTR referred to as sfRNA. Analysis of infections with a mutant Kunjin virus that is unable to generate appreciable amounts of the major sfRNA species indicated that RNAi suppression was associated with the generation of the non-coding sfRNA. Co-immunoprecipitation of sfRNA with RNAi mediators Dicer and Ago2 sugges...
Source: Virology - August 28, 2015 Category: Virology Authors: Moon SL, Dodd BJ, Brackney DE, Wilusz CJ, Ebel GD, Wilusz J Tags: Virology Source Type: research

The hub protein loquacious connects the microRNA and short interfering RNA pathways in mosquitoes
Aedes aegypti mosquitoes vector several arboviruses of global health significance, including dengue viruses and chikungunya virus. RNA interference (RNAi) plays an important role in antiviral immunity, gene regulation and protection from transposable elements. Double-stranded RNA binding proteins (dsRBPs) are important for efficient RNAi; in Drosophila functional specialization of the miRNA, endo-siRNA and exo-siRNA pathway is aided by the dsRBPs Loquacious (Loqs-PB, Loqs-PD) and R2D2, respectively. However, this functional specialization has not been investigated in other dipterans. We were unable to detect Loqs-PD in Ae....
Source: Nucleic Acids Research - April 19, 2015 Category: Research Authors: Haac, M. E., Anderson, M. A. E., Eggleston, H., Myles, K. M., Adelman, Z. N. Tags: Molecular Biology Source Type: research

GSE65070 The hub protein Loqs connects the miRNA and endo-siRNA pathways in mosquitos
Contributors : Mary E Haac ; Michelle A Anderson ; Heaher L Eggleston ; Kevin M Myles ; Zach N AdelmanSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Aedes aegyptiAedes aegypti mosquitoes vector several arboviruses of global health significance, including dengue viruses and chikungunya virus. RNA interference (RNAi) plays an important role in antiviral immunity, gene regulation and protection from transposable elements. Double-stranded RNA binding proteins (dsRBPs) are important for efficient RNAi; in Drosophila functional specialization of the miRNA, endo-siRNA and exo-siRNA pathway is aided...
Source: GEO: Gene Expression Omnibus - April 8, 2015 Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Aedes aegypti Source Type: research

KDEL Receptors Assist Dengue Virus Exit from the Endoplasmic Reticulum
In this study, we demonstrate that dengue virus (DENV) interacts with KDEL receptors (KDELR), which cycle between the ER and Golgi apparatus, for vesicular transport from ER to Golgi. Depletion of KDELR by siRNA reduced egress of both DENV progeny and recombinant subviral particles (RSPs). Coimmunoprecipitation of KDELR with dengue structural protein prM required three positively charged residues at the N terminus, whose mutation disrupted protein interaction and inhibited RSP transport from the ER to the Golgi. Finally, siRNA depletion of class II Arfs, which results in KDELR accumulation in the Golgi, phenocopied result...
Source: Cell Reports - March 6, 2015 Category: Cytology Source Type: research

Kinome siRNA screen identifies novel cell-type specific dengue host target genes
Publication date: October 2014 Source:Antiviral Research, Volume 110 Author(s): Yong-Jun Kwon , Jinyeong Heo , Hazel E.E. Wong , Deu John M. Cruz , Sumathy Velumani , Camila T. da Silva , Ana Luiza P. Mosimann , Claudia N. Duarte dos Santos , Lucio H. Freitas-Junior , Katja Fink Dengue is a global emerging infectious disease, with no specific treatment available. To identify novel human host cell targets important for dengue virus infection and replication, an image-based high-throughput siRNA assay screening of a human kinome siRNA library was conducted using human hepatocyte cell line Huh7 infected with a recent dengue...
Source: Antiviral Therapy - November 13, 2014 Category: Infectious Diseases Source Type: research

siRNAs encapsulated in Recombinant Capsid protein derived from Dengue serotype 2 virus inhibits the four serotypes of the virus and proliferation of cancer cells
In this study we have evaluated the utility of the protein as an siRNA carrier for anti Dengue viral and anti cancer applications using cell culture systems. We show that target specific siRNAs delivered using the capsid protein inhibit infection by the four serotypes of Dengue virus and proliferation of two cancer cell lines. Our data confirm the potential of the capsid for anti Dengue viral and anti cancer RNAi applications. In addition, we have optimized a fermentation strategy to improve the yield of E. coli expressed D2C protein since the reported yield of E.coli expressed flaviviral capsid proteins are low.
Source: Journal of Biotechnology - November 13, 2014 Category: Biotechnology Source Type: research