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Infectious Disease: Dengue Fever
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Total 65 results found since Jan 2013.
Abstracts of Presentations at the Association of Clinical Scientists 143 < sup > rd < /sup > Meeting Louisville, KY May 11-14,2022
Conclusion: These assays are suitable for routine diagnostic. The UltraFast NextGenPCR is the fastest with average time (30mins), followed by Agilent (2 hrs) and MassArray (6hrs). Upon completion of this activity, participants should be able to examine, measure and compare results from different assays for SARS detection, evaluate and diagnose accurately, as well as being able to plan, organize and recommend a diagnostic procedure for diagnostic laboratory. Key words: SARS-CoV-2, RNA extraction, RT-PCR, limit of detection, quantification cycle, COVID-19, in vitro diagnostic tests, Agilent, Massarray, Ultrafast. [20] From t...
Source: Annals of Clinical and Laboratory Science - July 1, 2022 Category: Laboratory Medicine Source Type: research
Dengue virus is involved in insulin resistance via the downregulation of IRS-1 by inducing TNF- α secretion
Biochim Biophys Acta Mol Basis Dis. 2022 Jun 22:166472. doi: 10.1016/j.bbadis.2022.166472. Online ahead of print.ABSTRACTDuring the epidemic, the individuals with underlying diseases usually have a higher rate of mortality. Diabetes is highly prevalent worldwide, making it a frequent comorbidity in dengue fever patients. Therefore, understanding the relationship between dengue virus (DENV) infection and diabetes is important. We first demonstrated that DENV-3 infection down-regulated the expression of IRS-1. In vitro, treatment of HepG2 cells with TNF-α inhibitors and siRNA proved that after DENV-3 infection in HepG2 cell...
Source: Biochimica et Biophysica Acta - June 25, 2022 Category: Biochemistry Authors: Xuling Liu Zuxin Liang Hongwei Duan Jianhai Yu Zhiran Qin Jingshu Li Li Zhu Qinghua Wu Weiwei Xiao Chenguang Shen Chengsong Wan Kefeng Wu Hua Ye Bao Zhang Wei Zhao Source Type: research
The < i > Aedes aegypti < /i > siRNA pathway mediates broad-spectrum defense against human pathogenic viruses and modulates antibacterial and antifungal defenses
by Yuemei Dong, Shengzhang Dong, Nahid Borhani Dizaji, Natalie Rutkowski, Tyler Pohlenz, Kevin Myles, George Dimopoulos
The mosquito’s innate immune system defends against a variety of pathogens, and the conserved siRNA pathway plays a central role in the control of viral infections. Here, we show that transgenic overexpression of Dicer2 (Dcr2) orR2d2 resulted in an accumulation of 21-nucleotide viral sequences that was accompanied by a significant suppression of dengue virus (DENV), Zika virus (ZIKV), and chikungunya virus (CHIKV) replication, thus indicating the broad-spectrum antiviral response mediated by the siRNA ...
Source: PLoS Biology: Archived Table of Contents - June 9, 2022 Category: Biology Authors: Yuemei Dong Source Type: research
Viruses, Vol. 14, Pages 339: In Vitro Inhibition of Replication of Dengue Virus Serotypes 1 & ndash;4 by siRNAs Bound to Non-Toxic Liposomes
The objective herein was to design and evaluate in vitro siRNAs bound to liposomes that inhibit DENV replication. siRNAs were designed against DENV1&ndash;4 from conserved regions using siDirect2.0 and Web-BLOCK-iT&trade; RNAiDesigner; the initial in vitro evaluation was carried out through transfection into HepG2 cells. siRNA with silencing capacity was encapsulated in liposomes composed of D-Lin-MC3-DMA, DSPC, Chol. Cytotoxicity, hemolysis, pro-inflammatory cytokine release and antiviral activity were evaluated using plaque assay and RT-qPCR. A working concentration of siRNA was established at 40 nM. siRN...
Source: Viruses - February 7, 2022 Category: Virology Authors: Rodriguez-Salazar Recalde-Reyes Bedoya Padilla-Sanabria Casta ño-Osorio Giraldo Tags: Article Source Type: research
The multifaceted biology of PCSK9
This article reviews the discovery of PCSK9, its structure-function characteristics, and its presently known and proposed novel biological functions. The major critical function of PCSK9 deduced from human and mouse studies, as well as cellular and structural analyses, is its role in increasing the levels of circulating LDL-cholesterol (LDLc), via its ability to enhance the sorting and escort of the cell surface LDL receptor (LDLR) to lysosomes. This implicates the binding of the catalytic domain of PCSK9 to the EGF-A domain of the LDLR. This also requires the presence of the C-terminal Cys/His-rich domain (CHRD), its bind...
Source: Endocrine Reviews - October 9, 2021 Category: Endocrinology Authors: Nabil G Seidah Annik Prat Source Type: research
Dengue virus induces Interferon- β by activating RNA sensing pathways in megakaryocytes
Immunol Lett. 2021 Jun 7:S0165-2478(21)00095-X. doi: 10.1016/j.imlet.2021.06.001. Online ahead of print.ABSTRACTActivation of innate receptors in megakaryocytes (MKs) may affect the ability to produce functional platelets. Low platelet count is one of the clinical manifestations of dengue virus (DENV) infection. In MKs, the effect of innate receptors during DENV-infection is not well studied. Here we used MEG-01 cells to investigate DENV serotype 2 induced innate receptors in these cells. DENV RNA was estimated by qRT-PCR in the culture supernatant. The expression of innate receptors was determined by western blot and qPCR...
Source: Immunology Letters - June 10, 2021 Category: Allergy & Immunology Authors: Ravi Prakash Arya Anismrita Lahon Ashok Kumar Patel Source Type: research
