Coagulation Panel in Patients with SARS-CoV2 Infection (COVID-19).
DISCUSSION: Coagulation function in patients with SARS-CoV2 infection is significantly deranged compared with normal patients. FIB and D-Dimer/FDP are the most significantly altered values and the early deetection of alteration could be useful to address therapies. D-Dimer/FDP (DD/FDP) alteration correlates with severity. Markedly elevated D-Dimer can be used to guide the introduction of anticoagulation therapy and evaluate prognosis of COVID-19. In every patient admitted with SARS-CoV2 infection PT, FIB, D-Dimer/FDP, and platelets must be ordered. We suggest daily extraction for every patient admitted and tested positive ...
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: Guevara-Noriega KA, Lucar-Lopez GA, Nuñez G, Rivera-Aguasvivas L, Chauhan I Tags: Ann Clin Lab Sci Source Type: research

Clinical Characteristics and Blood Test Results in COVID-19 Patients.
CONCLUSIONS: Results of blood tests have potential clinical value in COVID-19 patients. PMID: 32581016 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: An XS, Li XY, Shang FT, Yang SF, Zhao JY, Yang XZ, Wang HG Tags: Ann Clin Lab Sci Source Type: research

Morphoproteomics and Etiopathogenic Features of Pulmonary COVID-19 with Therapeutic Implications: A Case Study.
CONCLUSION: Morphoproteomics and microanatomical features coincide with the etiopathogenic features of pulmonary coronavirus infection and the host response. This suggests that a targeted therapy could address the biology of COVID-19 pneumonia, enhance the host immune response and prevent its progression to a life-threatening, ventilator-dependent clinical situation. PMID: 32581017 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: Brown RE, Wolf DA, Hunter RL, Zhao B, Buja LM Tags: Ann Clin Lab Sci Source Type: research

Could Demodex Be Used to Augment a Topical Vaccine Against Coronavirus (and Similar Viruses)?
PMID: 32581018 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: Strobel SL Tags: Ann Clin Lab Sci Source Type: research

Durable Clinical and Biochemical but Not Endoscopic Remission in Pediatric Crohn's Disease on Specific Carbohydrate Diet Monotherapy.
We report three pediatric CD patients who achieved clinical remission on SCD monotherapy, but failed to demonstrate complete mucosal healing with a minimum of one year follow up. This case report highlights that SCD monotherapy in male pediatric CD patients can sustain durable clinical and biochemical remission, but not full mucosal healing. PMID: 32581019 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: Britto SL, Kellermayer R Tags: Ann Clin Lab Sci Source Type: research

Reduction of Drug-Drug Interaction Risk; CDC Guidelines Influence on Opiate Benzodiazepine Prescribing.
We examined the results of 1.3 million drug tests performed on patients being monitored for compliance with pain medications and substance abuse rehabilitation to determine if the 2016 CDC prescribing guidelines had any impact on opiate benzodiazepine use. We observed that the combination of the opiate drugs morphine, oxycodone, and hydrocodone with the benzodiazepine metabolites oxazepam, alphahydroxyalprazolam, and 7-aminoclonazepam showed many patients were on a combination of these drugs. This ranged from approximately 9 to 16%. There was considerable variability between opiate drug pairs, but there was a general trend...
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: Pesce A, Thomas R, Krock K, Tran K, Ackerman G, Nickley J Tags: Ann Clin Lab Sci Source Type: research

Flow Cytometric Analysis of Monocytes and Granulocytes May Be Useful in the Distinction of Myeloid Neoplasms from Reactive Conditions: A Single Institution Experience and Literature Review.
CONCLUSIONS: There are significant and consistent differences in immunophenotype of monocytes and granulocytes in neoplastic groups versus controls. Immunophenotypic evaluation of monocytes and granulocytes in addition to blasts may be useful in flow cytometric assessment of minimal residual disease in myeloid neoplasms. PMID: 32581021 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: Brown LE, Zhang D, Cui W Tags: Ann Clin Lab Sci Source Type: research

Morphologic Classification Is an Important Prognostic Factor in Patients with Newly Diagnosed Multiple Myeloma Treated with Bortezomib or Lenalidomide.
This study shows that the visual morphological classification of plasma cells is an important prognostic factor even when bortezomib or lenalidomide is used as induction therapy. Further research is expected to reveal the optimal treatment strategy for immature and plasmablastic MM. PMID: 32581022 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: Takakuwa T, Araki T, Nakamura K, Fukuyama T, Miura A, Fujitani Y, Hisanabe A, Kaieda A, Fukada E, Yamamura R Tags: Ann Clin Lab Sci Source Type: research

Effects of MDM4 Gene Regulation by miR-34a on LOVO Cell Proliferation.
This study aims to investigate the effects of regulation of murine double minute protein4 (MDM4) expression by microRNA (miR)-34a on the proliferation of colorectal cancer LOVO cells. Prediction results obtained using a gene microarray showed that MDM4 is a specific miR-34a target gene. The interaction between miR-34a and the 3'-untranslated region (UTR) of MDM4 was detected using a luciferase reporter system. The effect of miR-34a on MDM4 protein expression was detected by Western blotting, and the effect of miR-34a transfection on LOVO cell proliferation was detected using an MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 dipheny...
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: Li S, Pei Y, Zhang W, Sun L, Jiang M, Shang G, Niu S, Ma Z Tags: Ann Clin Lab Sci Source Type: research

An Endoscopic Biopsy Specimen Contains Adequate Lymphocytes for Flow Cytometric Analysis of Light Chain Expression in the Gastrointestinal Mucosa.
CONCLUSIONS: An adequate number of lymphocytes for flow cytometry could be isolated from a single specimen of endoscopic mucosal biopsy from 93.3% of the patients. Overall, the sensitivity of flow cytometric analysis of light chain expression for the diagnosis of B-cell lymphoma was 83.3%, and the specificity was 100%. Although further investigation is required as the sample size of the present study was small, our study suggests a potential option for diagnosing B-cell lymphoma in the gastrointestinal mucosa. PMID: 32581024 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: Iwamuro M, Matsueda K, Takahashi T, Omote S, Tanaka T, Ennishi D, Otsuka F, Yoshino T, Okada H Tags: Ann Clin Lab Sci Source Type: research

Evi-1 Influence on Clinical Progress of Colorectal Cancer Patients.
CONCLUSION: The present study shows that Evi-1 expression is negatively correlated with JNK and c-Jun expression, and closely related to some clinical data. Further, Evi-1 combined with JNK and c-Jun can be used for adverse prognosis of CRC. PMID: 32581025 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: Zhu Y, Zhang J, Ye Z, Peng P, Lan X, Xie L, Qin M, Huang J Tags: Ann Clin Lab Sci Source Type: research

Renal Impairment Increases Serum Cancer Antigen 125 Levels in Patients with Type 2 Diabetes Mellitus.
CONCLUSIONS: Serum CA125 levels are positively correlated with urinary microalbumin and 24-hours urinary protein in T2DM patients; renal impairment may increase serum CA125 levels in T2DM patients. PMID: 32581026 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: Peng YF, Sun JF, Li L Tags: Ann Clin Lab Sci Source Type: research

C-Reactive Protein and Blood Gas Analysis in Cases of Neonatal Encephalopathy Treated with Therapeutic Hypothermia.
Abstract OBJECTIVE: Neonatal encephalopathy (NE) is one of the important causes of mortality and morbidity today. Therapeutic hypothermia (TH) applied to moderate and severe NE patients has neuroprotective effects. The role of C-reactive protein (CRP) in determining the clinical severity of NE is not clear. METHODS: Medical records of 118 NE patients treated with TH were reviewed. The patients were divided into two groups as CRP positive (CRP-P) (≥1 mg/dL) and CRP negative (CRP-N) (
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: Kazanasmaz H, Gumus H Tags: Ann Clin Lab Sci Source Type: research

Dynamically Expressed miR-BART16 Functions as a Suppressor of CAND1 in Infectious Mononucleosis Caused by Epstein-Barr Virus in Children.
CONCLUSIONS: These results highlight that EBV-miR-BART16 plays an important role in regulating the expression of CAND1 to affect pediatric IM. PMID: 32581028 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: Hu Z, Zheng X, Zeng W, Wang H, Yang H, Fan Y Tags: Ann Clin Lab Sci Source Type: research

Long Non-Coding RNA-ATB Attenuates the Angiotensin II-Induced Injury of Vascular Endothelial Cell.
CONCLUSION: The findings indicated that lncRNA-ATB might be associated with the development of the cardiovascular disease. PMID: 32581029 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: Wang M, Gan S, Li B, Wang Y Tags: Ann Clin Lab Sci Source Type: research

Trust your Endocrinologist - Report and Recommendations on the Ordering of Reverse T3 Testing.
Abstract Laboratory testing for markers of thyroid function is essential for the diagnosis of thyroid disease, yet, the landscape of thyroid function testing is complex and inappropriate test orders are common. Reverse T3 (rT3) is frequently seen on thyroid function testing menus as a marker of nonthyroidal illness. However, the diagnostic utility of rT3 for this indication is questionable, and testing of rT3 is not recommended by any professional practice guidelines. We reviewed a set of rT3 orders at our institution, and identified that 11 of 20 orders appeared inappropriate with respect to clinical context. The...
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: Garnett ER, Pagaduan JV, Devaraj S Tags: Ann Clin Lab Sci Source Type: research

Intestinal Spirochetosis: Case Series and Review of the Literature.
CONCLUSIONS: IS is usually an incidental histologic finding, and the association with symptoms is still unclear. The clinical presentation could be very diverse, hence, a long list of differential diagnosis should be ruled out. Additional clinical testing should be pursued if patients are unresponsive to antibiotic treatment. PMID: 32581031 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: Tong YT, Younes M Tags: Ann Clin Lab Sci Source Type: research

Rare Epididymal-Derived Myoepithelial Carcinoma: A Case Report and Review of Literature.
We report the first case of MC derived from the epididymis. The current reports of non-parotid MC are still rare, and epididymal-derived MC has not been reported previously. Simultaneously, we explore the role of EWSR1 fusion as a predicting marker and further reveal the origin of MC to provide new ideas for its diagnosis and treatment. PMID: 32581032 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: Wang K, Shi H, Mao W, Li W, Wu Z, Wang G, Yin L, Xie J, Peng B Tags: Ann Clin Lab Sci Source Type: research

Rare Case of Diffuse Large B-Cell Lymphoma Mimicking Acute Monocytic Leukemia, Associated with Complex Karyotype.
CONCLUSION: To date, no case reports of a patient diagnosed with DLBCL mimicking acute monocytic leukemia with complex karyotype have been reported. We present the case given its rarity, easy misdiagnosis, and poor prognosis. The case highlights the importance of awareness about the rare morphological variant to laboratory staff and hematologists. PMID: 32581033 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: Chen X, Liu K, Hu J, Fan J, Huo Q, Yin J Tags: Ann Clin Lab Sci Source Type: research

Myelodysplastic Syndrome with t(1;14),t(1;17),t(1;19) Transforms to AML-M5: A Case Report and Literature Review.
We report a case of a 62-year-old male patient diagnosed with MDS with excess blasts. The karyotype was 45, XY,+1,+1,-7,-10,-22,t(1;14) (q21;q32),t(1;17)(q21;p13),t(1;19)(q21;p13). The patient and his family refused treatment for financial reasons. After 2 months, the patient's MDS transformed into acute myeloid monocytic leukemia (AML-M5). This case of MDS with poor prognosis shows that patients with chromosomal numerical abnormality and balanced translocations should be treated early to prevent transition to AML. Further study of this case will reveal the molecular mechanism of MDS-to-AML transformation and identify new ...
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: Zhang H, Li G, Chang J, Wang H Tags: Ann Clin Lab Sci Source Type: research

Outdate Reduction and Cost Savings with Rapid Testing for Seven-Day Platelet Storage.
Abstract Platelets for transfusion in the US are stored at room temperature which is associated with a risk of bacterial transmission and subsequent sepsis. A recent FDA Final Guidance has been issued with options to mitigate this risk while maintaining or enhancing platelet availability.Storage had been limited to five days for many years due to the risk of bacterial growth. The short shelf-life has resulted in a national outdate rate of approximately 16%. FDA has recently cleared two devices as "safety measures" the use of which now allows seven-day platelet storage in bags cleared for this option. The...
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: Mintz PD, Sanders JR Tags: Ann Clin Lab Sci Source Type: research

Environmental Pollution, Oxidative Stress and Thioretinaco Ozonide: Effects of Glyphosate, Fluoride and Electromagnetic Fields on Mitochondrial Dysfunction in Carcinogenesis, Atherogenesis and Aging.
Abstract Environmental pollutants, such as pesticides, herbicides, additives to food and water, and electromagnetic fields threaten public health by promotion of cancer, heart disease and chronic diseases of aging. Many of these pollutants cause adverse health outcomes by effects on mitochondrial function to produce oxidative stress through loss of the active site complex for oxidative phosphorylation, thioretinaco ozonide oxygen nicotinamide adenine dinucleotide phosphate, from opening of the mitochondrial permeability transition pore. Glyphosate, fluoride, and electromagnetic fields are examples of carcinogenic ...
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: McCully KS Tags: Ann Clin Lab Sci Source Type: research

Evaluation of New Monoclonal Anti-MyoD1 (MX049) for the Diagnosis of Rhabdomyosarcoma: Comparison with 5.8A, EP212, Anti-Desmin, Anti-Myogenin, and Fluorescence in situ Hybridization.
Abstract Rhabdomyosarcoma (RMS) is a primitive embryonal mesenchymal neoplasm demonstrating skeletal muscle differentiation. Diagnosis of RMS remains difficult due to the diversity of clinical features, pathological forms, and lesion's locations. Immunohistochemistry and Fluorescence in Situ Hybridization are common methods used to aid RMS diagnosis. In this research we tested protein expression of Desmin (Clone MX046), MyoD1 (Clone MX049), MyoD1 (Clone 5.8A), MyoD1 (Clone EP212), Myogenin (Clone F5D), and cytogenetic features in 21 RMS cases, with following results: positive rates of Desmin (Clone MX046), MyoD1 (...
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: He Y, Chen X, Yu X Tags: Ann Clin Lab Sci Source Type: research

Analytical and Clinical Evaluation of Chemiluminescent Carcinoembryonic Antigen (CEA) by HISCL-5000 Immunoanalyzer.
CONCLUSIONS: CEA by HISCL-5000 immunoassay provided reliable performance. Comorbidities should be considered for interpretation of CEA. PMID: 32581038 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - May 1, 2020 Category: Laboratory Medicine Authors: Jekarl DW, Lee S, Park HI, Chae HJ, Kim M, Kim Y Tags: Ann Clin Lab Sci Source Type: research

Herbert Derman, MD (1921-2019).
PMID: 32366551 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - March 1, 2020 Category: Laboratory Medicine Authors: Derman E, Derman J, Thall L, Hawker C Tags: Ann Clin Lab Sci Source Type: research

C-reactive Protein Is a Predictor of Prognosis of Prostate Cancer: A Systematic Review and Meta-Analysis.
This study was conducted to explore the association between C-reactive protein (CRP) and survival of prostate cancer (PCa). An electronic search was completed on the basis of PubMed, Embase and Cochrane Central Register of Controlled Trials. The hazard ratio (HR) and 95% confidence interval (CI) were extracted. Studies evaluating the relation between pretreatment levels of CRP and survival of PCa were included. Sixteen articles incorporating 17,833 patients were eligible for the present meta-analysis. Elevated pretreatment CRP level was significantly associated with poorer overall survival (OS) (HR=1.58, 95% CI 1.31-1.91, P
Source: Annals of Clinical and Laboratory Science - March 1, 2020 Category: Laboratory Medicine Authors: Liao DW, Hu X, Wang Y, Yang ZQ, Li X Tags: Ann Clin Lab Sci Source Type: research

Anti-TNF- α Biologics Do Not Reverse CDX2 Downregulation in Patients with Crohn's Disease.
CONCLUSIONS: Although CDX2 is downregulated in CD, it did not revert to normal in patients treated with anti-TNF-α biologics. PMID: 32366553 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - March 1, 2020 Category: Laboratory Medicine Authors: Younes M, Rahimi E, DuPont AW, Ly CJ, Ertan A Tags: Ann Clin Lab Sci Source Type: research

Computer-assisted Diagnosis of Breast Cancer by Cell Network Matrix Extraction and Multilayer Perceptron Analysis.
CONCLUSION: This study shows that using a combination of cell-graph extraction and a deep learning algorithm computers can accurately distinguish between benign and malignant breast lesions. PMID: 32366554 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - March 1, 2020 Category: Laboratory Medicine Authors: Boroujeni AM, Yousefi E, Haseeb MA, Gupta R Tags: Ann Clin Lab Sci Source Type: research

MiR-1247-5p Functions as a Tumor Suppressor in Human Astroglioma Cells by Targeting CDC14B.
In this study, we investigated the effect of miR-1247-5p on glioma progression. miR-1247-5p showed a low expression in glioma cell lines and glioma tissues from patients. The ectopic overexpression of miR-1247-5p significantly inhibited the proliferation and invasion of U251 cells, induced apoptosis in vitro, and inhibited the growth of transplanted tumor cells in vivo CDC14B was found to be a potential target of miR-1247-5p. The overexpression of miR-1247-5p can significantly down-regulate the expression of CDC14B by directly targeting the 3'UTR of this gene. CDC14B can promote the degradation of p53 and inhibit its activ...
Source: Annals of Clinical and Laboratory Science - March 1, 2020 Category: Laboratory Medicine Authors: Liu Q, Dong Z, Chen T Tags: Ann Clin Lab Sci Source Type: research

Effects of Oxysterols on Chondrogenesis of Human Adipose-derived Stem Cells.
This study aims to investigate the effect of oxysterols on the chondrogenesis of hADSCs. hADSCs were collected from the abdominal subcutaneous tissue samples of patients undergoing caesarean section, and were cultured to passage 5. Mesenchymal stem cell markers were examined by flow cytometry. After the cells were subjected to adipogenic, osteogenic, and chondrogenic induction, the differentiation of each cell lineage was evaluated by RT-PCR and specific staining (Oil red O, Alizarin red S, and Alcian blue, respectively). The cell pellets of hADSCs were cultured in chondrogenic induced media containing 2μM 22(R)-hydroxy...
Source: Annals of Clinical and Laboratory Science - March 1, 2020 Category: Laboratory Medicine Authors: Kim MW, An BM, Wang L, Lee KY, Bang J, Joo BS Tags: Ann Clin Lab Sci Source Type: research

Ki-67 and Clinical Correlations in Patients with Resistant Prolactinomas.
Abstract OBJECTIVE: Drug resistance is the main limitation for dopamine agonist therapy with prolactinoma. Transsphenoidal surgery is an additional operation that circumvents this limitation. To characterize distinctive clinical features of resistant prolactinoma in different levels of Ki-67, a retrospective analysis of 423 patients who were diagnosed and surgically treated at Shandong Provincial Hospital Affiliated to Shandong University from January 2007 to June 2017 was performed. METHODS: Patients were subdivided into Ki-67≥3% group (High) and Ki-67
Source: Annals of Clinical and Laboratory Science - March 1, 2020 Category: Laboratory Medicine Authors: Lu C, Liu Y, Lu Z, Huan C Tags: Ann Clin Lab Sci Source Type: research

Taxotere-induced WNT16 Expression in Carcinoma-Associated Fibroblasts Might Associate with Progression and Chemoresistance of Breast Cancer.
The objective of this study is to investigate the relationship between WNT16 expression in carcinoma-associated fibroblasts and Taxotere treatments in breast cancer. PROCEDURES: WNT16 expression in primarily cultured carcinoma-associated fibroblasts was detected via Real-Time PCR and Western blot. A total of 106 breast cancer cases were prepared for immunohistochemistry. Multiple assays were performed to examine the function of breast cancer cells (MDA-MB-231) co-cultured with carcinoma-associated fibroblasts when WNT16 was inhibited. RESULTS: Enhanced WNT16 expression was verified in carcinoma-associated fibrobl...
Source: Annals of Clinical and Laboratory Science - March 1, 2020 Category: Laboratory Medicine Authors: Xiang L, Song Z, Rong G Tags: Ann Clin Lab Sci Source Type: research

Plasmablastic Lymphomas: Characterization of Tumor Microenvironment Using CD163 and PD-1 Immunohistochemistry.
This study aims to characterize the tumor microenvironment of plasmablastic lymphoma (PBL) in regard to the quantities of CD163(+) tumor associated macrophages (TAM) and PD1(+) tumor infiltrating lymphocytes (TIL). This article also reviews the existing knowledge of the role of PD-1/PD-L1 pathway in the tumor microenvironment of hematopoietic neoplasms, discusses potential mechanisms to explain our findings, and outlines areas for future studies. We performed CD163 and PD1 immunohistochemical studies in 11 cases classified as plasmablastic lymphoma, and recorded the percentages of positive TAMs and TILs. Based on previous ...
Source: Annals of Clinical and Laboratory Science - March 1, 2020 Category: Laboratory Medicine Authors: Ahn JS, Al-Habib A, Vos JA, Sohani AR, Barboza-Quintana O, Flores JP, Wen S, Rosado FG Tags: Ann Clin Lab Sci Source Type: research

Circulating miRNA-199a and miRNA-122 Levels as Potential Diagnostic and Prognostic Biomarkers for Hepatocellular Carcinoma.
In this study, the miR-122 and miR-199a expression levels were determined by quantitative real-time PCR (qRT-PCR). The function and molecular mechanism of miR-122 and miR-199a target genes were analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Correlation between miRNA expression levels and the overall survival (OS) of HCC patients was assessed by Kaplan-Meier analysis. RESULTS: Serum miR-122 expression had no significant difference between patients with HCC (HCC patients) and healthy controls (HCs), whereas serum miR-199a expression was significantly lower in HCC patients than the HCs (p
Source: Annals of Clinical and Laboratory Science - March 1, 2020 Category: Laboratory Medicine Authors: Wu J, Wu Y, Luo Y, Li X, Lin N, Yang X, Lin Y, Chen M Tags: Ann Clin Lab Sci Source Type: research

Efficacy of Serum Total Bilirubin in Predicting the Severity of Ulcerative Colitis: A Cross-Sectional Study.
Abstract OBJECTIVE: Several serum parameters can be used to assess ulcerative colitis (UC) disease activity. However, the value of these parameters for predicting UC severity has not been studied in depth. Therefore, we sought to investigate the value of serum total bilirubin (TB) as a predictor of UC severity. MATERIALS AND METHODS: This cross-sectional study included 448 UC patients and 308 healthy participants. Data regarding the serum levels of TB, hemoglobin (Hb), albumin (Alb), erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP) were collected. UC severity was evaluated according to the T...
Source: Annals of Clinical and Laboratory Science - March 1, 2020 Category: Laboratory Medicine Authors: Zhang MH, Wang HG, Shi YT, Zhou JF, Yan W, Ma TH, Wu SN, Yang XZ Tags: Ann Clin Lab Sci Source Type: research

The Role of Serum Calcium in Assessing Metabolic Syndrome in Colorectal Cancer.
CONCLUSIONS: Serum calcium, especially total calcium, might be more sensitive than FPG and lipids for metabolic syndrome in CRC patients. PMID: 32366562 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - March 1, 2020 Category: Laboratory Medicine Authors: Cao LL, Cai J, Yue Z, Pei L, Wang H, Jia M Tags: Ann Clin Lab Sci Source Type: research

Distribution of Carbapenemases and Efflux Pump in Carbapenem-resistance Acinetobacter baumannii.
In this study, 100 isolates of carbapenem-resistance in Acinetobacter baumannii (CRAB) were collected from clinical specimens. Agar dilution was conducted to determine the minimum inhibitory concentrations (MICs) for 15 kinds of antibiotics. Genes of carbapenemases and efflux pumps were amplified by PCR. The expression difference of pump genes was analyzed by real-time PCR between CRAB and carbapenems-sensitive Acinetobacter baumannii (CSAB). We found that most antibiotics, including aminoglycosides, fluoroquinolones, and cephalosporins showed high MIC values in CRAB. All isolates were sensitive to polymyxin B. Among the C...
Source: Annals of Clinical and Laboratory Science - March 1, 2020 Category: Laboratory Medicine Authors: Zhu LJ, Pan Y, Gao CY, Hou PF Tags: Ann Clin Lab Sci Source Type: research

Associations of Anti-MHC Class I-related Chain A (MICA) Antibodies with Renal Allograft Outcomes.
Abstract The association of anti-MHC class I-related chain A (MICA) and kidney allograft rejection has been reported. However, the associations of antibodies specific to donor MICA (dsMICA) and dsMICA levels with allograft outcome have not been elucidated. From November 2009 to June 2017, 125 sera samples from renal transplantation recipients with no HLA antibodies were screened for MICA antibodies. Patients with positive MICA antibody screening results and available residual donor DNA for MICA geno-typing underwent dsMICA analysis. Among these 125 sera samples, 19 were positive for MICA antibodies. dsMICA was pos...
Source: Annals of Clinical and Laboratory Science - March 1, 2020 Category: Laboratory Medicine Authors: Lee N, Nam M, Roh EY, Shin S, Park KU, Song EY Tags: Ann Clin Lab Sci Source Type: research

Red Blood Cell Distribution Width Is Increased in Patients with Pemphigus: A Case-Control Study.
Abstract BACKGROUND: Red blood cell distribution width (RDW) has recently emerged as an inflammatory marker in several inflammatory diseases but has not been investigated in patients with pemphigus. OBJECTIVE: We aimed to examine RDW percentage in patients with pemphigus relative to control subjects and to assess the association between this biomarker and the morphological characteristics of the disease. METHODS: This case-control study included 183 pemphigus patients and 915 age- and sex-matched control subjects. RDW, hemoglobin, and mean corpuscular volume (MCV) were measured for all study participants...
Source: Annals of Clinical and Laboratory Science - March 1, 2020 Category: Laboratory Medicine Authors: Britva RL, Kridin M, Kridin K Tags: Ann Clin Lab Sci Source Type: research

Correlation of Fentanyl Positive Drug Screens with Other Medications in Patients from Pain, Rehabilitation and Behavioral Programs.
We examined 1.3 million urine drug tests from April 2016 to April 2019 for fentanyl and other drugs. The highest relationship was observed with heroin. Approximately 30%-40% of the drug tests positive for the heroin metabolite 6-monacetylmorphine (6-MAM) were also positive for fentanyl. There was a large variance over time, but the percent positive in 2016 and 2019 were similar. In contrast, there was a definite increase in the presence of fentanyl with cocaine and methamphetamine. There was not a large increase over time associated with methadone, buprenorphine, and marijuana. PMID: 32366566 [PubMed - in process] (So...
Source: Annals of Clinical and Laboratory Science - March 1, 2020 Category: Laboratory Medicine Authors: Krock K, Nickley J, Tran K, Greg Ackerman RT, Pesce A Tags: Ann Clin Lab Sci Source Type: research

Morphoproteomics Identifies CXCR4 in Undifferentiated Colorectal Cancer: A Case Study with Therapeutic Implications.
Abstract Undifferentiated colorectal cancer (UCRC) has a well-documented CD44 cancer stem cell (CSC) component. CXCR4 (C-X-C motif receptor 4) also is a CSC marker and with its ligand, stromal-derived factor (SDF)-1 alpha has been shown to act in concert with CD44 to promote cancer cell invasion. To date, CXCR4 has not been reported in the MEDLINE (PubMed) literature with respect to UCRC. Morphoproteomic analysis, utilizing bright field microscopy, can visualize the intensity and cell location of protein analytes with a patient's tumor including CXCR4 expression and was applicable to this case study. Using morphop...
Source: Annals of Clinical and Laboratory Science - March 1, 2020 Category: Laboratory Medicine Authors: Brown RE, Ali YD, Cai Z Tags: Ann Clin Lab Sci Source Type: research

Cryptic Acute Promyelocytic Leukemia (APL) Presenting as Seizures in an Adolescent.
Abstract There are approximately 800 new cases of acute promyelocytic leukemia (APL) in the United States every year. APL is rarely observed in pediatric populations, and accounts for less than 5-10% of all pediatric cases of acute myeloid leukemia (AML). APL typically presents with symptoms related to the pancytopenia such as fatigue due to anemia, bleeding and bruising secondary to thrombocytopenia, and infections attributed to a lack of functioning leukocytes. The presentation of APL in the central nervous system (CNS) is a rare phenomenon. In addition, APL has a dismal prognosis when found in the CNS. In this ...
Source: Annals of Clinical and Laboratory Science - March 1, 2020 Category: Laboratory Medicine Authors: Mai B, Liang C, Nguyen A, Wahed A, Chen L Tags: Ann Clin Lab Sci Source Type: research

Clinical Utility of HIV-1/2 Ab Immunochromatographic Assay as an Additive Test for HIV Screen-Positive Patients.
CONCLUSIONS: ICA for anti-HIV Ab screen-positive specimens is helpful for earlier medical decision considering frequent false-positive results on screening. Nonetheless, ICA might be negative in acute HIV syndrome, necessitating p24 Ag assay or NAT with shorter window periods. PMID: 32366569 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - March 1, 2020 Category: Laboratory Medicine Authors: Yoo SJ Tags: Ann Clin Lab Sci Source Type: research

Analytical Performance Evaluation of Aptima Herpes Simplex Virus 1 and 2 Assay Using Hologic Panther Instrument.
CONCLUSIONS: Aptima Herpes simplex assay on the Hologic Panther instrument is suitable for rapid detection of herpes simplex virus in clinical laboratory. PMID: 32366570 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - March 1, 2020 Category: Laboratory Medicine Authors: Dixon RB, Bonaparte J, Greenwood CJ, Davis M, Dasgupta A Tags: Ann Clin Lab Sci Source Type: research

Evaluation of the Automated Indirect Immunofluorescence Test for Antinuclear Antibodies.
Abstract The performance of the automated indirect immunofluorescence system was compared with the manual method for detection of antinuclear antibodies (ANA) from 354 clinical serum samples. We compared the results (negative or positive), ANA patterns, and titers for the two methods. The coincidence rates for ANA positive and negative samples were 93.4% and 98.7%, respectively. The coincidence rates for single patterns, mixed patterns, and the final titers were 85.1%, 87.6%, and 87.6%, respectively. The homogeneous, speckled, cytoplasmic, centromere, multiple nuclear dots, and nucleolar pattern coincidence rates ...
Source: Annals of Clinical and Laboratory Science - March 1, 2020 Category: Laboratory Medicine Authors: Wu S, Liu F, Wang F, Huang J, Yin B, Huang M, Wang T, Wei N Tags: Ann Clin Lab Sci Source Type: research

The Biology of Pulmonary Coronavirus Infection Underscores Host-Directed Therapies as an Option.
PMID: 32366572 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - March 1, 2020 Category: Laboratory Medicine Authors: Brown RE, Hunter RL Tags: Ann Clin Lab Sci Source Type: research

The Effects of Follistatin on the Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Neurons-Like Cells.
Abstract OBJECTIVE: The present study was designed to evaluate the effects of Follistatin (FST) on the differentiation of human bone marrow mesenchymal stem cells (hBMSCs) into neuron-like cells. MATERIALS AND METHODS: hBMSCs were isolated and characterized by cell surface markers including CD29, CD44, CD166, CD34, CD14, and CD45. Subsequently, 0.3, 3, and 10 nmol/L recombinant human FST (rhFST) were used to stimulate hBMSCs, respectively. Neuron-like cell differentiation and Nissl's body within the cytoplasm of hBMSCs were investigated by a transmission electron microscope (TEM). Meanwhile, nestin and NSE we...
Source: Annals of Clinical and Laboratory Science - January 1, 2020 Category: Laboratory Medicine Authors: Li W, Zhang L, Yin X, Ai H Tags: Ann Clin Lab Sci Source Type: research

FZD8 Indicates a Poor Prognosis and Promotes Gastric Cancer Invasion and Metastasis via B-Catenin Signaling Pathway.
In conclusion, our findings show that FZD8 promotes GC invasion and metastasis via the β-catenin pathway. PMID: 32161008 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - January 1, 2020 Category: Laboratory Medicine Authors: Chen W, Liu Z, Mai W, Xiao Y, You X, Qin L Tags: Ann Clin Lab Sci Source Type: research

CAPN6 Regulates Uterine Leiomyoma Cell Proliferation and Apoptosis through the Rac1-Dependent Signaling Pathway.
CONCLUSIONS: This data suggests that CAPN6 regulates UtLMs proliferation and apoptosis while being mediated through the Rac1/PAK1 signaling pathway. PMID: 32161009 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - January 1, 2020 Category: Laboratory Medicine Authors: Zhu L, Sun Y, Wu Q, Zhang C, Ling J Tags: Ann Clin Lab Sci Source Type: research

Yeast Extract Induces Apoptosis and Cell Cycle Arrest via Activating p38 Signal Pathway in Colorectal Cancer Cells.
Abstract The microbiome has recently become a key interest for cancer research. Anti-tumor effects of reinforced clostridium media (RCM) were investigated for all ingredients of RCM, which showed that yeast extract could be a candidate for this phenomenon. MTT assay, cell counting, cell death analysis, cell cycle analysis, and Western blotting were done on colorectal cancer cells with or without 5-fluorouracil resistance (SNU-C5 and SNU-C5/5-FUR). Yeast extract treatment showed dose- and time-dependent anti-tumor effects on SNU-C5 and SNU-C5/5-FUR. Anti-tumor effects were related to G0/G1 phase arrest with increas...
Source: Annals of Clinical and Laboratory Science - January 1, 2020 Category: Laboratory Medicine Authors: Moon D, Kang HK, Kim J, Yoon SP Tags: Ann Clin Lab Sci Source Type: research