Automated Diagnosis of Lymphoma with Digital Pathology Images Using Deep Learning.
Abstract Recent studies have shown promising results in using Deep Learning to detect malignancy in whole slide imaging, however, they were limited to just predicting a positive or negative finding for a specific neoplasm. We attempted to use Deep Learning with a convolutional neural network (CNN) algorithm to build a lymphoma diagnostic model for four diagnostic categories: (1) benign lymph node, (2) diffuse large B-cell lymphoma, (3) Burkitt lymphoma, and (4) small lymphocytic lymphoma. Our software was written in Python language. We obtained digital whole-slide images of Hematoxylin and Eosin stained slides of ...
Source: Annals of Clinical and Laboratory Science - March 1, 2019 Category: Laboratory Medicine Authors: Achi HE, Belousova T, Chen L, Wahed A, Wang I, Hu Z, Kanaan Z, Rios A, Nguyen AND Tags: Ann Clin Lab Sci Source Type: research

Role of Platelet Microparticles in Blood Diseases: Future Clinical Perspectives.
Abstract Platelet microparticles (PMPs) are released from the resting or activated platelet membrane, which includes a variety of proteins, mRNA, miRNA, lipids, and other substances. PMPs are involved in cell communication in vivo and are potential markers for multiple diseases. This narrative review focuses on the extraction, identification, and intercellular role of PMPs, with emphasis on the lesser known aspects of PMPs, namely, PMPs and complement systems, and their role in some diseases and disorders of women such as miscarriage and polycystic ovary syndrome, as well as the application of proteomics. For clin...
Source: Annals of Clinical and Laboratory Science - March 1, 2019 Category: Laboratory Medicine Authors: Chen F, Liao Z, Peng D, Han L Tags: Ann Clin Lab Sci Source Type: research

Clinical and Prognostic Value of Human Mammary Tumor Virus in Korean Patients with Breast Carcinoma.
Abstract BACKGROUND: Human mammary tumor virus (HMTV) is 90-95% homologous to mouse mammary tumor virus, one of the causal agents of murine mammary tumors. Although HMTV has been frequently detected in human breast cancers, its clinical and prognostic value remains unknown. METHODS: In the present study, we analyzed HMTV infection using polymerase chain reaction (PCR) in 128 breast cancers. RESULTS: HMTV was found in 9.4% (12/128) of breast cancers and was significantly associated with breast pain (66.7% vs. 11.7%, p=0.007). It had a tendency to be detected more frequently in breast cancer patients with lower BMI
Source: Annals of Clinical and Laboratory Science - March 1, 2019 Category: Laboratory Medicine Authors: Seo I, Cho JH, Lee MH, Park WJ, Kwon SY, Lee JH Tags: Ann Clin Lab Sci Source Type: research

Effects of the Dibutyl Phthalate (DBP) on the Expression and Activity of Aromatase in Human Granulosa Cell Line KGN.
This study suggests that DBP may affect the steroidogenic capacity in human ovaries and contributes to our understanding of the effects of DBP on the female reproductive system. PMID: 31028061 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - March 1, 2019 Category: Laboratory Medicine Authors: Ma Y, Zhang J, Zeng R, Qiao X, Cheng R, Nie Y, Luo Y, Li S, Zhang J, Xu W, Xu L, Hu Y Tags: Ann Clin Lab Sci Source Type: research

Pizotifen Inhibits the Proliferation and Migration of Colon Cancer HCT116 Cells by Down-regulating Wnt Signaling Pathway.
CONCLUSION: Pizotifen may inhibit HCT116 cells proliferation and migration by suppressing Wnt signaling pathway and it may serve as a potential candidate drug for the treatment of colon cancer in the future. PMID: 31028062 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - March 1, 2019 Category: Laboratory Medicine Authors: Piao SS, Shang B Tags: Ann Clin Lab Sci Source Type: research

Expression of HDAC4 in Stage B Hepatocellular Carcinoma and Its Influence on Survival.
CONCLUSIONS: Our study shows that HDAC4 over-expression is important for the development of advanced HCC, which may serve as a useful prognostic biomarker and therapeutic target for this malignancy. PMID: 31028063 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - March 1, 2019 Category: Laboratory Medicine Authors: Wang Z, Wang H, Shen P, Xie R Tags: Ann Clin Lab Sci Source Type: research

N-acetyl-beta-D-Glucosaminidase in Tissues of Rats Chronically Exposed to Cadmium and Treated with Ozone.
Abstract Our aims were to evaluate N-acetyl-beta-D-glucosaminidase (NAG) activity in an experimental rat model of chronic exposure to cadmium and its response to ozone therapy. Forty male Wistar rats were divided into five groups: control, cadmium only, cadmium and oxygen, cadmium and ozone, and ozone only. Cadmium concentration (ASA method) and NAG activity (by the Maruhn method) were determined in the supernatants of the kidneys, liver, and pancreas. The histopathological alterations were evaluated in tissue sections.The highest concentration of cadmium and NAG activity was observed in rats intoxicated with cadm...
Source: Annals of Clinical and Laboratory Science - March 1, 2019 Category: Laboratory Medicine Authors: Śliwińska-Mossoń M, Sobiech K, Dolezych B, Madej P, Milnerowicz H Tags: Ann Clin Lab Sci Source Type: research

Detection of Plasma Catecholamines in Human Pheochromocytoma and Primary Hypertension Based on Liquid Chromatography Tandem Mass Spectrometry.
CONCLUSIONS: Our results show that the combined detection of the seven CAs/derivatives could be used for the clinical diagnosis of PPGL and PH. PMID: 31028065 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - March 1, 2019 Category: Laboratory Medicine Authors: Xue X, Chen H, Xia Q, He P, Lv Y, Liu P, Yang Y, Wu C, Gao Q, Peng J, Yu Y, Wang F, Qu W, Wang Z Tags: Ann Clin Lab Sci Source Type: research

A Simple and Accurate Liquid Chromatography-Tandem Mass Spectrometry Method for Therapeutic Drug Monitoring of Busulfan in Plasma.
In this study, we developed a simple and accurate method for measuring busulfan concentrations using liquid chromatography tandem mass spectrometry (LC-MS/MS). METHODS: Separation and detection of busulfan was performed using T3 column equipped with LC-MS/MS. Busulfan was isolated from 50 μL human plasma after mixing with busulfan-2H8 (internal standard) solution, calibrator, and quality-control material. The sample was eluted and gradated with a mobile phase composed of ammonium acetate, formic acid, and water or methanol. The busulfan concentration was quantified using a six-point standard curve. Busulfan and bus...
Source: Annals of Clinical and Laboratory Science - March 1, 2019 Category: Laboratory Medicine Authors: Lee EJ, Park N, Lee SH, Lee W, Kim HS, Chun S, Min WK Tags: Ann Clin Lab Sci Source Type: research

The Association between Serum Uric Acid Levels and Insulin Resistance and Secretion in Prediabetes Mellitus: A Cross-Sectional Study.
Abstract BACKGROUND: We investigated the association between serum uric acid (SUA) levels and insulin secretion and resistance in prediabetes patients, which is currently unclear. METHODS: Our study population consisted of 11,523 representative patients from northwest China. It was divided into three groups based on the results of an oral glucose tolerance test: the non-diabetes group (n=3234), the impaired glucose regulation (IGR, prediabetes) group (n=2886), and the type-2 diabetes mellitus group (n=5403). SUA and 2-hour insulin (2h-Ins) levels were measured, and homeostasis model assessment of insulin resi...
Source: Annals of Clinical and Laboratory Science - March 1, 2019 Category: Laboratory Medicine Authors: Wu Y, He H, Yu K, Zhang M, An Z, Huang H Tags: Ann Clin Lab Sci Source Type: research

Investigation of the Potential Use of Sialic Acid as a Biomarker for Rheumatoid Arthritis.
CONCLUSIONS: Higher serum levels of Neu5Ac were significantly associated with the presence as well as severity of RA. Neu5Ac may be a potential biomarker for prediction and severity of RA in clinical practice. PMID: 31028068 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - March 1, 2019 Category: Laboratory Medicine Authors: Li W, Liu Y, Zheng X, Gao J, Wang L, Li Y Tags: Ann Clin Lab Sci Source Type: research

Association of Methylenetetrahydrofolate Reductase (MTHFR) A1298C Polymorphism with Lower High-Density Lipoprotein Cholesterol Level.
CONCLUSION: Our study suggests that MTHFR C667T gene polymorphism is not related to any components of metabolic syndrome. MTHFR 1298 variants were significantly associated with lower HDL level compared to MTHFR 1298 wild type. PMID: 31028069 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - March 1, 2019 Category: Laboratory Medicine Authors: Mahesh M, Cheng G, Khalighi K Tags: Ann Clin Lab Sci Source Type: research

Serum C1q Concentration Positively Correlates with Erythrocyte Sedimentation Rate in Polymyositis/Dermatomyositis.
This study aimed to investigate C1q concentrations in the sera of PM/DM patients. C1q concentrations were measured in the sera of 87 PM/DM patients and 100 healthy subjects. In addition, the association between C1q concentration and laboratory indexes such as CK, AST, ALT, LDH, hsCRP, and ESR were also evaluated. Our data indicated no significant difference in the serum C1q concentration between PM/DM patients and healthy subjects. However, serum C1q concentrations were observed to be significantly higher in PM/DM patients in patients group with elevated ESR than in those with normal ESR (207.55±37.54 mg/L and 180.6...
Source: Annals of Clinical and Laboratory Science - March 1, 2019 Category: Laboratory Medicine Authors: Li L, Chen J, Chen S, Liu C, Zhang F, Li Y Tags: Ann Clin Lab Sci Source Type: research

Serum Sphingolipidomic Analysis in Acne Vulgaris Patients.
CONCLUSIONS: Future studies are needed to understand the role of altered sphingolipid levels in the pathophysiology of AV. PMID: 31028071 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - March 1, 2019 Category: Laboratory Medicine Authors: Kaya S, Aslan İ, Kıraç E, Karaarslan T, Aslan M Tags: Ann Clin Lab Sci Source Type: research

Effect of TREM2 on Release of Inflammatory Factor from LPS-stimulated Microglia and Its Possible Mechanism.
CONCLUSIONS: Downregulating the expression of TREM2 can inhibit the release of inflammatory factors from LPS-stimulated microglia by inhibiting NF-κB signaling pathway activity, which may be beneficial for the treatment of SCI. PMID: 31028072 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - March 1, 2019 Category: Laboratory Medicine Authors: Wang M, Gao X, Zhao K, Chen H, Xu M, Wang K Tags: Ann Clin Lab Sci Source Type: research

Protective Effects of Erythropoietin towards Acute Lung Injuries in Rats with Sepsis and Its Related Mechanisms.
Abstract The aim of this study was to explore the effects of erythropoietin (EPO) in acute lung injuries in rats with sepsis. 127 male SD rats were divided into 3 groups (n=8): group Sham, group ALI (sepsis-caused lung injury), group EPO (EPO intervention). The blood gas analysis, C-reactive protein level (CRP), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-10 were detected and compared. The water content fraction and myeloperoxidase (MPO) activities, apoptosis, and expression level of nuclear factor -κB (NF-κB p65) in lung tissues were also detected. Compared to group Sham, ...
Source: Annals of Clinical and Laboratory Science - March 1, 2019 Category: Laboratory Medicine Authors: Zhang X, Dong S Tags: Ann Clin Lab Sci Source Type: research

Spinal Cord Syphilitic Gumma Presenting with Brown-S équard Syndrome: A Case Report and Literature Review.
CONCLUSION: Spinal syphilitic gumma exhibits diverse clinical manifestations, lacks specific imaging features, accompanied by the patient's history deliberately concealed. Since clinicians do not have sufficient knowledge about such rare cases, misdiagnosis and missed diagnosis will be likely. When there is clinical suspicion for spinal syphilitic gumma, clinicians should pay close attention to relevant medical history, carry out a comprehensive physical examination and specific serological tests and cerebrospinal fluid (CSF) analysis. In summary, in cases with stable neurologic conditions, a trial administration of intrav...
Source: Annals of Clinical and Laboratory Science - March 1, 2019 Category: Laboratory Medicine Authors: Huang YH, Shi QX, Xu MM, Chen CZ, Yang ML, Li JJ, Chen YF, Lin ZQ, Lin YY Tags: Ann Clin Lab Sci Source Type: research

Technical Note: Analytical Performance Evaluation of Electrochemiluminescence Cyclosporine Assay on Cobas e411 Analyzer.
CONCLUSION: The new electrochemiluminescence cyclosporine assay is comparable to the FDA approved CMIA tacrolimus assay. Therefore, when this assay is approved by the FDA, it can be used for therapeutic drug monitoring of cyclosporine. PMID: 31028075 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - March 1, 2019 Category: Laboratory Medicine Authors: Dasgupta A, Khalil SA Tags: Ann Clin Lab Sci Source Type: research

Target Sequencing Identifies a Novel Mutation of ZFPM2 in a Chinese Newborn with Tetralogy of Fallot.
PMID: 31028076 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - March 1, 2019 Category: Laboratory Medicine Authors: Zhu L, Yue JW, Yang XH Tags: Ann Clin Lab Sci Source Type: research

Retraction.
Authors: PMID: 31028077 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - March 1, 2019 Category: Laboratory Medicine Tags: Ann Clin Lab Sci Source Type: research

IGF-1 Promotes Endocytosis of Alveolar Epithelial Cells through PI3K Signaling.
We report that IGF-1 increases endocytosis in a mouse alveolar epithelial cell line, MLE-12. The PI3K-Akt pathway is involved in this effect of IGF-1. Furthermore, IGF-1 can inhibit the production of interleukin-6 in lipopolysaccharide-stimulated AECs. We have found that IGF-1 can enhance endocytosis of AECs through the PI3K pathway and exhibit anti-inflammatory properties. These two observations suggest that IGF-1 is a potential mediator in the regulation of airway inflammation. PMID: 30814071 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - January 1, 2019 Category: Laboratory Medicine Authors: Wang H, He J, Luo Y, Mu M, Guo S, Shen L, Qian Z, Fang Q, Song C Tags: Ann Clin Lab Sci Source Type: research

Therapeutic Effect of the Quinolone Levofloxacin on Inflammatory Fibroblast-like Synoviocytes.
CONCLUSIONS: The anti-inflammatory and anti-proliferative effects of levofloxacin on FLS are mediated by inhibiting inflammatory cytokines and inducing apoptosis in rabbits. Thus, quinolones might possess a special effect to treat synovitis and relieve inflammatory joint disease. PMID: 30814072 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - January 1, 2019 Category: Laboratory Medicine Authors: Liu LM, Song JT, Gao F, Zhang H Tags: Ann Clin Lab Sci Source Type: research

Effects of the Modified Bazhen Decoction in the Treatment of Premature Ovarian Failure in Rats.
CONCLUSION: XIAP is involved in the regulation of oocyte and granulosa cells via the cysteinyl aspartate specific proteinase (caspase) pathway, and plays an important role in POF. MBD can dramatically activate XIAP, but inhibit the expression of miR-23a and miR-27a; preventing the apoptosis of oocyte and granulosa cells. Our study suggests that MBD may be a useful traditional Chinese medicine for the treatment of POF. PMID: 30814073 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - January 1, 2019 Category: Laboratory Medicine Authors: Liu C, Li Q, Yang Y Tags: Ann Clin Lab Sci Source Type: research

The Effect of Triggering Receptor Expressed by Myeloid Cells 2 Modified Bone Marrow Mesenchymal Stem Cells on Alzheimer's Disease-Mouse Model.
CONCLUSIONS: TREM2 modified bone marrow MSCs affected the ability of learning and memory of AD model mice and this mechanism may be related to the expression of TREM2 and DAP12 genes. PMID: 30814074 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - January 1, 2019 Category: Laboratory Medicine Authors: Yang Y, Wu X, Qu X, Wang X, Luo C, Li L, Zhang K Tags: Ann Clin Lab Sci Source Type: research

Dexmedetomidine Attenuates Renal and Myocardial Ischemia/Reperfusion Injury in a Dose-Dependent Manner by Inhibiting Inflammatory Response.
CONCLUSION: DEX could attenuate renal and myocardial I/R injury induced by renal I/R in a dose-dependent manner, at least in part, through its inhibitory effects on inflammatory response. PMID: 30814075 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - January 1, 2019 Category: Laboratory Medicine Authors: Xu Z, Wang D, Zhou Z, Chen Q, Zhang D, Chen S, Jiang H, Jia C, Liu X Tags: Ann Clin Lab Sci Source Type: research

Reproductive Systemic Toxicity and Mechanism of Glucosides of Tripterygium wilfordii Hook. F. (GTW).
CONCLUSION: Tripterygium glycosides affected the estrus cycle in female rats and caused damage to the uterus; in male rats, the testicles and sperm were damaged. The mechanism of reproductive toxicity occurred through the secretion of multiple hormones. PMID: 30814076 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - January 1, 2019 Category: Laboratory Medicine Authors: Guo J, Huang Y, Lei X, Zhang H, Xiao B, Han Z, Liang C, Yang W Tags: Ann Clin Lab Sci Source Type: research

Epitope Analysis Aids in Transplant Decision Making by Determining the Clinical Relevance of Apparent Pre-Transplant Donor Specific Antibodies (DSA).
Abstract Pre-transplantation work-up on a patient with end stage renal disease using Single Antigen Bead (SAB) testing showed significant anti-HLA-B*44:02 (>5,000 MFI) and anti-HLA-B*44:03 (>1,000 MFI) antibodies, with persistence on quarterly testing. No significant Class II anti-HLA antibodies were present. The patient received a potential offer from a living unrelated-donor expressing HLA-B*44:02. Based on the presence of anti-HLA-B*44:02 antibody, the crossmatch (XM) was predicted to be positive. However, the actual fluorescence cytometry crossmatch (FCXM) was negative. FCXM and Complement Dependent Cyto...
Source: Annals of Clinical and Laboratory Science - January 1, 2019 Category: Laboratory Medicine Authors: Pandey S, Harville TO Tags: Ann Clin Lab Sci Source Type: research

Serum 14-3-3 η Could Improve the Diagnostic Rate of Rheumatoid Arthritis and Correlates to Disease Activity.
This study aimed to evaluate the diagnostic capacity of serum 14-3-3η and its correlation with clinical variables in patients with RA. METHODS: A total of 94 patients with RA and 80 age- and sex-matched controls, including 40 healthy subjects, were included. Serum 14-3-3η levels were assessed by quantitative enzyme-linked immunosorbent assay. Receiver-operating characteristic (ROC) curves analysis was used to determine the sensitivity and specificity of 14-3-3η. Spearman's rank correlation coefficient was used to assess the relationship between 14-3-3η and other clinical measures in patients with RA. ...
Source: Annals of Clinical and Laboratory Science - January 1, 2019 Category: Laboratory Medicine Authors: Guan SZ, Yang YQ, Bai X, Wang Y, Feng KQ, Zhang HJ, Dong M, Yang HW, Li HQ Tags: Ann Clin Lab Sci Source Type: research

Dioscin Attenuates Myocardial Damages in Diabetic Rats maybe by Regulating NO-sGC-cGMP-PKG Pathway.
In conclusion, ITS medium has the potential to be used for the expansion of hAMSCs before clinical application. PMID: 30814079 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - January 1, 2019 Category: Laboratory Medicine Authors: Liu X, Zhang T, Wang R, Shi P, Pan B, Pang X Tags: Ann Clin Lab Sci Source Type: research

Influence of Docosahexaenoic Acid on Proliferation and Apoptosis in Human HepG2 Cell Line.
This study aims to investigate the effect of different concentrations of docosahexaenoic acid (DHA) on proliferation and apoptosis in HepG2 cell lines, and to research the possible molecular mechanisms. METHODS: DHA concentration was 0 g/mL in the negative control group, and 15, 30, 45, 60 and 75 ug/mL, respectively, in the experimental groups. CCK-8 and flow cytometry methods were used to observe the growth inhibition and apoptosis rates of HepG2 cells cultured in vitro, which were treated with different concentrations of DHA. The level of β-catenin and c-myc mRNA and protein were measured by real-time PCR and w...
Source: Annals of Clinical and Laboratory Science - January 1, 2019 Category: Laboratory Medicine Authors: Chen YJ, Jiang HT, Wang TF Tags: Ann Clin Lab Sci Source Type: research

Complement Component 3 as a Surrogate Hallmark for Metabolic Abnormalities in Patients with Chronic Hepatitis C.
CONCLUSIONS: These results suggest that the elevation of serum C3 levels may reflect obesity, insulin resistance, and/or hepatic steatosis in patients with CH-C, and that the increase in the synthesis of C3 may derive from the activation of cells other than hepatocytes in those patients. PMID: 30814081 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - January 1, 2019 Category: Laboratory Medicine Authors: Himoto T, Hirakawa E, Fujita K, Sakamoto T, Nomura T, Morishita A, Yoneyama H, Haba R, Masaki T Tags: Ann Clin Lab Sci Source Type: research

Role of Red Blood Cell Distribution Width in Predicting Cardiac Syndrome X in Women.
Abstract OBJECTIVE: To investigate whether the increased red blood cell distribution width (RDW) is a biomarker for predicting the presence of cardiac syndrome X (CSX) in women. METHODS: 110 CSX women patients and 98 normal controls were prospectively enrolled in this study. The CSX group consisted of patients with anginal chest pain, ischemia on noninvasive stress test and a normal coronary angiography. The control group was selected from the patients with anginal symptoms but those who had a normal stress test and a normal coronary angiography. Blood samples were drawn from all women patients for measuring ...
Source: Annals of Clinical and Laboratory Science - January 1, 2019 Category: Laboratory Medicine Authors: Zhao K, Li Y Tags: Ann Clin Lab Sci Source Type: research

Fatal Intracranial Hemorrhage in a Young Pregnant Patient with Acute Promyelocytic Leukemia.
In this study, we present a case of catastrophic intracranial hemorrhage in a young pregnant patient with the finding of leukemic involvement of the placenta. To our knowledge, this is the first confirmed case of acute promyelocytic leukemia involving the placenta. The clinical history, pertinent histological findings, and clinical outcomes will be discussed. PMID: 30814083 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - January 1, 2019 Category: Laboratory Medicine Authors: Zhang L, Tomsula J, Garcia A, Wahed A, Nguyen N, Chen L Tags: Ann Clin Lab Sci Source Type: research

Dioscin Attenuates Myocardial Damages in Diabetic Rats maybe by Regulating NO-sGC-cGMP-PKG Pathway.
The objective of this research was to explore the effect of dioscin on myocardium in streptozotocin (STZ)-induced diabetic rats and the underlying mechanisms. METHODS: Diabetic rat model was established by a single intravenous injection of streptozocin (STZ). The rats were divided into 5 groups: control group, control+dioscin group, model group (diabetes), DDL group (diabetic rats treated with 100 μg/kg/day dioscin) and DDH group (diabetic rats treated with 200 μg/kg/day dioscin). Each group was continuously intervened for 6 weeks. Hemodynamic parameters were detected and pathological alterations of myocardium w...
Source: Annals of Clinical and Laboratory Science - January 1, 2019 Category: Laboratory Medicine Authors: Wei Q, Zhu T, Xiao X, Sun L, Zhang Z, Huang T Tags: Ann Clin Lab Sci Source Type: research

Simvastatin and Simvastatin-Ezetimibe Improve the Neurological Function and Attenuate the Endothelial Inflammatory Response after Spinal Cord Injury in Rat.
Abstract During a spinal cord injury (SCI), mechanical trauma rapidly leads to a blood-spinal cord barrier (BSB) disruption, neural cell damage, axonal damage, and demyelination, followed by a cascade of secondary inflammatory reactions. These inflammatory responses spread the damage to the neural cells and impair the recovery of neurological functions. In the present study, we evaluated the efficacy of simvastatin and a simvastatin-ezetimibe combination therapy in managing the endothelial inflammatory response in an SCI rat model. Adult male Sprague-Dawley rats were group-housed and SCI was induced by using the m...
Source: Annals of Clinical and Laboratory Science - January 1, 2019 Category: Laboratory Medicine Authors: Liang CL, Chen HJ, Liliang PC, Wang HK, Tsai YD, Cho CL, Lu K, Wang KW Tags: Ann Clin Lab Sci Source Type: research

Significant Association of Methylenetetrahydrofolate dehydrogenase 1 Promoter Hypomethylation with Stroke in a Chinese Population with Primary Hypertension.
Abstract OBJECTIVE: Hypertension and hyperhomocysteinemia are both stroke risk factors. Methylenetetrahydrofolate dehydrogenase 1 (MTHFD1) plays a vital role in one-carbon metabolism via encoding a protein with three distinct enzymatic activities (methylenetetrahydrofolate dehydrogenase, methenyltetrahydrofolate cyclohydrolase, and formate-tetrahydrofolate ligase). We aimed to elucidate the association between MTHFD1 promoter methylation and stroke in a Chinese population with primary hypertension. METHODS: Quantitative methylation-specific PCR was used to quantify MTHFD1 methylation levels in 243 hypertensiv...
Source: Annals of Clinical and Laboratory Science - January 1, 2019 Category: Laboratory Medicine Authors: Wang C, Xu G, Wen Q, Peng X, Chen H, Zhang J, Xu S, Zhang C, Zhang M, Ma J, Hui Z, Du J, Wu G, Ma M Tags: Ann Clin Lab Sci Source Type: research

Proteomics Analysis of FLT3-ITD Mutation in Acute Myeloid Leukemia Using Deep Learning Neural Network.
In this study, we attempted to determine a set of critical proteins that were associated with the FLT3-ITD mutation in newly-diagnosed acute myeloid leukemia patients. A Deep Learning network consisting of autoencoders formed a hierarchical model from which high-level features were extracted without labeled training data. Dimensional reduction reduced the number of critical proteins from 231 to 20. Deep Learning found an excellent correlation between FLT3-ITD mutation with the levels of these 20 critical proteins (accuracy 97%, sensitivity 90%, and specificity 100%). Our Deep Learning network could hone in on 20 proteins w...
Source: Annals of Clinical and Laboratory Science - January 1, 2019 Category: Laboratory Medicine Authors: Liang CA, Chen L, Wahed A, Nguyen AND Tags: Ann Clin Lab Sci Source Type: research

Automated Versus Conventional Microscopic Interpretation of Antinuclear Antibody Indirect Immunofluorescence Test.
CONCLUSION: Although automated pattern recognition has some limitations, EUROPattern Suite facilitates automated ANA detection with a high sensitivity and concordance with conventional IIF. PMID: 30814088 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - January 1, 2019 Category: Laboratory Medicine Authors: Park Y, Kim SY, Kwon GC, Koo SH, Kang ES, Kim J Tags: Ann Clin Lab Sci Source Type: research

Vitamin D and Urinary Tract Infection: A Systematic Review and Meta-Analysis.
Abstract Whether or not Vitamin D deficiency is associated with urinary tract infections (UTI) remains controversial. We retrieved relevant articles from the PubMed, Google Scholar, the Cochrane Library, Med-line and Embase databases up to Mach 1, 2018 for studies investigating the association between Vitamin D and UTI. The meta-analysis of 9 studies included 1921 participants, of which 580 were diagnosed with UTI. They showed that Vitamin D insufficiency was associated with a significantly increased risk of having a UTI (pooled OR=3.01, 95%CI=2.31-3.91), with moderate heterogeneity (I2 =49.5%). Moreover, Vitamin ...
Source: Annals of Clinical and Laboratory Science - January 1, 2019 Category: Laboratory Medicine Authors: Deng QF, Chu H, Wen Z, Cao YS Tags: Ann Clin Lab Sci Source Type: research

The Association between Platelet Distribution Width and Stroke in Atrial Fibrillation Patients.
This study included 150 AF patients who were divided into stroke and non-stroke groups. Multivariate logistic regression analysis and area under the receiver operating characteristic curve were performed. RESULTS: Among the 150 patients, 50 and 100 were grouped into the stroke and non-stroke groups, respectively. There were significant differences in systolic blood pressure, total cholesterol, C-reactive protein, and PDW between the groups (P
Source: Annals of Clinical and Laboratory Science - January 1, 2019 Category: Laboratory Medicine Authors: Lyu QS, Liu B, Huang C, Huang YQ Tags: Ann Clin Lab Sci Source Type: research

The 139th Meeting of the Association of Clinical Scientists, May 15-18, 2019, Hershey, PA.
Authors: PMID: 30814091 [PubMed] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - January 1, 2019 Category: Laboratory Medicine Tags: Ann Clin Lab Sci Source Type: research

Prognostic Significance of Categorizing Gastric Carcinoma by PD-L1 Expression and Tumor Infiltrating Lymphocytes.
Abstract GOALS: We categorized gastric cancers into four types of tumor immune microenvironment based on PD-L1 expression and tumor-infiltrating lymphocytes (TILs) to analyze clinicopathologic characteristics and recommend an immunotherapy-targetable subgroup. PROCEDURES: Formalin-fixed, paraffin-embedded tissue samples of randomly selected gastric adenocarcinoma (n=479) were obtained and arrayed using a tissue-arraying instrument. Immunohistochemical stains for PD-L1, PD-1, and CD8 were performed and evaluated in addition to microsatellite instability, EBV infection, and HER-2 analyses. RESULTS: Tumor m...
Source: Annals of Clinical and Laboratory Science - November 1, 2018 Category: Laboratory Medicine Authors: Noh BJ, Kim JH, Eom DW Tags: Ann Clin Lab Sci Source Type: research

Increased Expression of Lncrna RP11-397A15.4 in Gastric Cancer and Its Clinical Significance.
Abstract BACKGROUND: Long noncoding RNAs (lncRNAs) have been demonstrated to have potential diagnostic and prognostic values for many types of cancers. However, their clinical value in patients with gastric cancer (GC) remains unknown. METHODS: The expression levels of lncRNA RP11-397A15.4 in 68 GC tissues and their matched adjacent non-tumor tissues from GC patients and in 24 normal mucosa or those with minimal gastritis (healthy controls) were determined by qRT-PCR. The expression levels of lncRNA RP11-397A15.4 in human GC cell lines and a normal gastric epithelium cell line were also detected by qRT-PCR. T...
Source: Annals of Clinical and Laboratory Science - November 1, 2018 Category: Laboratory Medicine Authors: Chen H, Xu Z, Liu X, Gao Y, Wang J, Qian P, Yang B Tags: Ann Clin Lab Sci Source Type: research

Upregulated Expression of Serum Exosomal microRNAs as Diagnostic Biomarkers of Lung Adenocarcinoma.
Conclusion: Serums ex-miR-21-5p, -126-3p, and -140-5p have great potential to serve as highly sensitive, stable, and repeatable biomarkers for early diagnosis of AC. However, larger cohort studies are necessary to validate these results. PMID: 30610040 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - November 1, 2018 Category: Laboratory Medicine Authors: Feng M, Zhao J, Wang L, Liu J Tags: Ann Clin Lab Sci Source Type: research

Downregulation of miR-196b Promotes Glioma Cell Sensitivity to Temozolomide Chemotherapy and Radiotherapy.
Abstract Human glioma is the most common type of primary brain tumor. The survival rate of people with a malignant glioma is extremely low, primarily due to a lack of effective treatments. We previously reported that miR-196b expression is upregulated in glioblastoma tissues and overexpression of miR-196b is associated with poor prognosis. miR-196b acts as an oncogene by enhancing cellular proliferation and increasing the expression of E2F1, which plays an important role in the PI3K/AKT signaling pathway. In the present study, we explored the effects of miR-196b expression on glioma cells and characterized the rel...
Source: Annals of Clinical and Laboratory Science - November 1, 2018 Category: Laboratory Medicine Authors: Ma R, Zheng G, Shao C, Liu J, Lv H, Zhang G Tags: Ann Clin Lab Sci Source Type: research

Algorithm to Estimate the Extended Turnaround Time Including Outpatient Waiting Time for Blood Specimen Collection when a Stand-alone Queue Ticket System not Connectable to Laboratory Information System Is Used.
Abstract BACKGROUND: A queue ticket system (QTS) used in an outpatient phlebotomy clinic was unable to be directly integrated with the laboratory information system (LIS). To monitor patient's waiting time and extended turnaround time (TAT) as patient-centered quality indicators for outpatient laboratory services, we developed an algorithm to integrate data between the QTS and the LIS. METHODS: Between June 1 to September 30, 2017, data files were exported from a QSYS-8000 (HION Tech, Seoul, Korea). Each calling event from the QTS data was matched to a barcode of test requests from the LIS if the following co...
Source: Annals of Clinical and Laboratory Science - November 1, 2018 Category: Laboratory Medicine Authors: Song YE, Kang H, Park H Tags: Ann Clin Lab Sci Source Type: research

3-Iodothyronamine Acting through an Anti-Apoptotic Mechanism Is Neuroprotective Against Spinal Cord Injury in Rats.
This study aims to show how 3-iodothyronamine (T1AM) protects against spinal cord injury (SCI) in rats. We randomly divided adult female Sprague-Dawley rats (N=54) into three equal groups: (1) untreated control (n=18) (2) T1AM (n=18) (3) T1AM+EPPTB (n=18). The clamp method was used to produce SCI at the T10 segment, and the following data were collected 3, 5, and 7 days after the injury plus treatment. The mean BBB scores of both the control and T1AM+EPPTB groups were 1.5±0.5, 3.5±0.5, and 4.5±0.5 on days 3, 5, and 7 after SCI, respectively, whereas those for the T1AM group were 3.3±0.5, 5.3&plu...
Source: Annals of Clinical and Laboratory Science - November 1, 2018 Category: Laboratory Medicine Authors: Lv J, Liao J, Tan W, Yang L, Shi X, Zhang H, Chen L, Wang S, Li Q Tags: Ann Clin Lab Sci Source Type: research

Role of Bone Marrow Mesenchymal Stromal Cells in Attenuating Inflammatory Reaction in Lipopolysaccaride-induced Acute Kidney Injury of Rats Associated with TLR4-NF- κB Signaling Pathway Inhibition.
This study was conducted to investigate whether BMSCs could alleviate the inflammation reaction in lipopolysaccaride (LPS)-induced acute kidney injury (septic-AKI) of rats via inhibition of toll-like receptors (TLR4)-nuclear factor-kappa B (NF-κB) signaling pathway. The septic-AKI rat model was established by injecting the 1ml/mg LPS through the femoral vein. Based on this model, rats were subjected to BMSC transplantation, PDTC (a kind of NF-κB inhibitor) administration alone, and combined treatment of the first two together. Results showed that LPS treatment caused the increases of the concentration of blood ...
Source: Annals of Clinical and Laboratory Science - November 1, 2018 Category: Laboratory Medicine Authors: Xiu GH, Zhou X, Li XL, Chen XZ, Li BQ, Chen XL, Jin H, Pan XH, Sun J, Ling B Tags: Ann Clin Lab Sci Source Type: research

A Clinical Trial on Oral H. pylori Infection of Preschool Children.
Abstract GOALS: Over the past twenty years, the existence of oral Helicobacter pylori (H. pylori) infection has been controversial and is still disputed. It proposes that living H. pylori do not exist in the oral cavity. However, the progressive loss of efficacy of standard eradication therapies has made the treatment of H. pylori more challenging than ever due to oral H. pylori infection. We conducted a study to explore the existence of oral H. pylori infection in preschool children. PROCEDURES: A total of 178 children were recruited and evaluated using the saliva H. pylori antigen test (HPS) and the urea br...
Source: Annals of Clinical and Laboratory Science - November 1, 2018 Category: Laboratory Medicine Authors: Xu Y, Song Y, Wang X, Gao X, Li S, Yee JK Tags: Ann Clin Lab Sci Source Type: research

The Relationship between Serum Total Bilirubin and Carotid Intima-Media Thickness in Patients with Prehypertension.
This study was designed to evaluate the relationship between total bilirubin and carotid intima-media thickness (cIMT), a marker of subclinical atherosclerosis, in patients with prehypertension. RESULTS: We consecutively enrolled 219 patients with prehypertension. All the participants were divided into two groups according to cIMT values (
Source: Annals of Clinical and Laboratory Science - November 1, 2018 Category: Laboratory Medicine Authors: Lyu QS, Huang YQ Tags: Ann Clin Lab Sci Source Type: research