Akt Downregulates B-Cell Translocation Gene-2 Expression Via Erk1/2 Inhibition for Proliferation of Cancer Cells.
Abstract B-cell translocation gene 2 (Btg2) is a tumor suppressor gene that is implicated in many biological processes. Akt is a serine/threonine kinase which was originally discovered as an oncogene. The prognostic value of Akt activation in some types of cancers and its effect on tumor suppressor genes remains to be fully elucidated. In the current research we have investigated the Akt-mediated downregulation of Btg2 that increased cells proliferation and cells survival. Human leukemia HL-60, THP-1 and colon cancer DLD-1 cells were used in this study. Inhibition of Akt with LY294002 significantly increased Btg2 ...
Source: Annals of Clinical and Laboratory Science - November 1, 2020 Category: Laboratory Medicine Authors: Zubair H, Lim IK, Safi SZ, Rehman F, Imran M Tags: Ann Clin Lab Sci Source Type: research

Overexpression of Sine Oculis Homeobox Homolog 2 Predicts Poor Survival and Clinical Parameters of Patients with Colon Adenocarcinoma.
In this study, we explored the prognostic value of Six2 in colon adenocarcinoma (COAD). METHODS: Wilcoxon signed-rank test and logistic regression were applied to identify relationship between clinical features and Six2 expression. The effect of Six2 expression and clinical features on the survival of COAD patients was explored using Kaplan-Meier and Cox regression analyses. Gene Set Enrichment Analysis (GSEA) was performed utilizing TCGA dataset. RESULTS: Compared to adjacent normal tissues, Six2 was highly expressed in COAD. Overexpression of Six2 in COAD was significantly associated with M classification (OR=2...
Source: Annals of Clinical and Laboratory Science - November 1, 2020 Category: Laboratory Medicine Authors: Jiang Y, Zhou B, Liang B, Zeng F, Zou Z Tags: Ann Clin Lab Sci Source Type: research

Altered Serum Annexin A2 Might Be a New Potential Diagnostic Biomarker in Human Colorectal Cancer.
This study aimed to investigate clinical utility of serum Annexin A2 as a potential biomarker for CRC. METHODS: Annexin A2 was analyzed in 20 cases of CRC tissues and 20 controls of normal adjacent paired tissues. Serum Annexin A2 was calculated in 59 CRC patients and 44 healthy subjects. Receiver operating characteristic (ROC) curve and logistic regression were utilized to evaluate the diagnostic effectiveness and construct diagnostic model. RESULTS: Annexin A2 in CRC tissues was slightly higher than in normal adjacent paired tissues (χ2=6.0652, p
Source: Annals of Clinical and Laboratory Science - November 1, 2020 Category: Laboratory Medicine Authors: Hu D, Shen B, Yu M, Zha X, Zhou Y, Chen F, Ren J, Zhang L Tags: Ann Clin Lab Sci Source Type: research

circ_0067934: A Potential Biomarker and Therapeutic Target for Hepatocellular Carcinoma.
Abstract Hepatocellular carcinoma (HCC) is a high-level invasive and metastatic malignancy with a low survival rate. Accumulating evidence has proved that circular RNAs (cirRNAs) function as potential biomarkers for diagnosis or prognosis in various cancer, including HCC. In the present study, we aimed to explore a novel biomarker, circ_0067934 for diagnosis and prognosis of HCC so as to contribute to therapeutic strategies. In the present study, the expression levels of circ_0067934 in HCC patients and paired controls were determined by real-time quantitative polymerase chain reaction (qRT-PCR) analysis. The corr...
Source: Annals of Clinical and Laboratory Science - November 1, 2020 Category: Laboratory Medicine Authors: Zhou C, Li R, Mi W Tags: Ann Clin Lab Sci Source Type: research

Anti-Cancer Effects of RAW 264.7 Cells on Prostate Cancer PC-3 Cells.
Abstract Macrophages have the potential to re-programing tumor cells in the tumor microenvironments. Thus we investigated anti-cancer effects of M1-polarized macrophages by lipopolysaccharide (LPS) on the physiological properties of human prostate cancer PC-3 cells. To identify communications with immune cells and tumor cells, we performed in-direct way by using conditioned-media (CM) and analyzed tumor properties via quantitative polymerase chain reaction, enzyme-linked immunosorbent assay and western blot and flow cytometry. CM of M1-polarized macrophages induced apoptotic cell death in PC-3 cells, and it surpri...
Source: Annals of Clinical and Laboratory Science - November 1, 2020 Category: Laboratory Medicine Authors: Nam HW, Bae J, Kim YW, An HH, Kim SH, Kim KY, Yu SN, Park BB, Lee SY, Ahn SC Tags: Ann Clin Lab Sci Source Type: research

Role of Estrogen Receptor-Mediated Anti-Tumor Effects in U2OS Cells.
This study aimed to investigate the effects of 17β-estradiol and estrogen receptors (ERs) in U2OS cells. METHODS: Osteosarcoma U2OS cells were divided into six groups, and cell proliferation was determined using the cell counting kit-8 growth test. Furthermore, U2OS cell migration and invasion were examined by cell scratch test and Transwell invasion assays, respectively. RESULTS: At 48 h of 17β-estradiol exposure, U2OS cell viability decreased (p
Source: Annals of Clinical and Laboratory Science - November 1, 2020 Category: Laboratory Medicine Authors: Yang Z, Qian J, Yu W, Yang M, Liu B, Tao H Tags: Ann Clin Lab Sci Source Type: research

Morphological and Immunohistochemical Features of Placental Damage in Cases of Perinatal Death: Institutional Experience with Emphasis on Viral Etiology.
CONCLUSION: Damage of placental vessels due to the influence of pathogenic factors (virus antigens, TNF) can cause acute or chronic intrauterine fetus hypoxia which is a leading pathogenetic factor of perinatal death. PMID: 33334790 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - November 1, 2020 Category: Laboratory Medicine Authors: Nasyrov RA, Sidorova NA, Melnikova VF, Fedotova EP Tags: Ann Clin Lab Sci Source Type: research

Regulatory Effects of Toll-like Receptor 4 Knockout on CD4+ and CD8+ T Lymphocytes and Interleukin-17 During Myocardial Ischemia.
In this study, C57BL/6 wild-type (WT) mice and TLR4 knockout mice were divided into three groups, including a normal control group, an MI group that was generated using high doses of isoproterenol (ISO), and an ISO+rHMGB1 group that was generated using a combination of ISO and recombinant HMGB1 (rHMGB1). Echocardiography, hematoxylin and eosin staining, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), and flow cytometry were used to examine each group. The results showed that rHMGB1 could further aggravate myocardial injury and increase the CD4+/CD8+ ratio and the expression level of interleukin-17 (IL-17) (p
Source: Annals of Clinical and Laboratory Science - November 1, 2020 Category: Laboratory Medicine Authors: Ren Y, Wang R, Jiang L, Wang X, Feng Z, He J, He X, Du G Tags: Ann Clin Lab Sci Source Type: research

Mechanism of Naringenin Blocking the Protection of LTB4/BLT1 Receptor Against Septic Cardiac Dysfunction.
CONCLUSION: Naringenin inhibits LTB4/BLT1 receptors to attenuate cardiomyocyte inflammation and apoptosis, which may mediate the protective effect of anti-septic cardiac dysfunction by activating AMPK signaling pathway and inhibiting NF-κB signaling and mitochondrial damage. PMID: 33334792 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - November 1, 2020 Category: Laboratory Medicine Authors: Ye G, Wang M, Liu D, Cheng L, Yin X, Zhang Q, Liu W Tags: Ann Clin Lab Sci Source Type: research

TRPV6 Is Associated with Prognosis of ST-Elevation Acute Myocardial Infarction.
CONCLUSION: The lower expression of TRPV6 was associated with poor clinical outcomes and prognosis of STEMI patients. PMID: 33334793 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - November 1, 2020 Category: Laboratory Medicine Authors: Zhang H, Ding P, Zheng L Tags: Ann Clin Lab Sci Source Type: research

Red Cell Depletion of Major ABO Incompatible Bone Marrow Hematopoietic Progenitor Cells HPC(M) with the Spectra Optia ®.
CONCLUSIONS: The Spectra Optia® is a viable option for depleting HPC(M) harvests of contaminating RBC in situations of ABO incompatibility. Target cells from a MNC rich fractionation were preserved through processing while eliminating RBC contaminants. PMID: 33334794 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - November 1, 2020 Category: Laboratory Medicine Authors: Christensen A, Hsieh F, Boyer M, Couriel D, Reems JA Tags: Ann Clin Lab Sci Source Type: research

Vascular Endothelial Growth Factor-A Is Associated with Platelets and Complement 4 in Patients with Primary Sj ögren's Syndrome.
Vascular Endothelial Growth Factor-A Is Associated with Platelets and Complement 4 in Patients with Primary Sjögren's Syndrome. Ann Clin Lab Sci. 2020 Nov;50(6):790-796 Authors: Huang ZY, Yuan YR, Kong YL, Zhang T, Liang Y Abstract OBJECTIVE: We aimed at investigating the expression level of vascular endothelial growth factor-A (VEGF-A) in patients with primary Sjögren's Syndrome (pSS) and evaluating the relationship between serum VEGF-A and the laboratory indicators that are associated with it in pSS. METHODS: The VEGF-A levels were measured by ELISA in a total of 88 participants, incl...
Source: Annals of Clinical and Laboratory Science - November 1, 2020 Category: Laboratory Medicine Authors: Huang ZY, Yuan YR, Kong YL, Zhang T, Liang Y Tags: Ann Clin Lab Sci Source Type: research

Dissemination of SHV, TEM and CTX-M Genotypes in Pseudomonas aeruginosa: A Pre-eminent Reason for Therapeutic Failure in Pediatrics.
Abstract OBJECTIVE: Dissemination of extended-spectrum beta-lactamase (ESBL) genotypes in Pseudomonas aeruginosa gained attention as superbugs, which are extremely difficult to treat with conventional antibiotics. The study aimed to delineate the empirical therapy and the occurrence of SHV, TEM, and CTX-M encoding P. aeruginosa among pediatric patients. METHODS: A total number of 33,400 continuous and non-repetitive clinical specimens collected from the debilitated pediatric patients processed for the isolation of P. aeruginosa. The characterization of the isolated strains performed using phenotypic and molec...
Source: Annals of Clinical and Laboratory Science - November 1, 2020 Category: Laboratory Medicine Authors: Ejaz H Tags: Ann Clin Lab Sci Source Type: research

Monomorphic Epitheliotropic Intestinal T-cell Lymphoma: A Study of Four Cases and Review of Literature.
Abstract Monomorphic epitheliotropic intestinal T-cell lymphoma (MEITL) is a rare primary and highly aggressive intestinal T-cell lymphoma derived from intraepithelial lymphocytes. MEITL is previously designated as type II enteropathy-associated T cell lymphoma (EATL). Unlike to classic form of EATL, MEITL is not associated with celiac disease. The diagnosis of MEITL is very challenging and the clinical outcome of patients with MEITL is very poor. Herein we describe a series of four patients diagnosed with MEITL identified upon a 10-year institutional retrospective review. Histopathologic examination of these case...
Source: Annals of Clinical and Laboratory Science - November 1, 2020 Category: Laboratory Medicine Authors: Fei F, Reddy V, Patel CR, Dhall D, Lee G, Meng-Jun X, Al Diffalha S Tags: Ann Clin Lab Sci Source Type: research

Leiomyoadenomatoid Tumor of Epididymis: A Variant of Adenomatoid Tumor.
Abstract Adenomatoid tumor is a rare tumor of mesothelial origin, usually arising in the epididymis. It is the most common paratesticular tumor of middle-aged men. A rare variant of adenomatoid tumor is leiomyoadenomatoid tumor which is characterized by prominent spindle cell myoblastic and myofibroblastic proliferation in the background of an adenomatoid tumor with tubular spaces lined by mesothelial cells. In some cases, the spindle cell component obscures the adenomatoid tumor component, complicating accurate diagnosis. Here, we report two cases of paratesticular leiomyoadenomatoid tumor in 28-year-old and 50-y...
Source: Annals of Clinical and Laboratory Science - November 1, 2020 Category: Laboratory Medicine Authors: Shehabeldin A, Al Sannaa G, El-Zaateri Z, Andino L, Schmolze D, Chapman D, Ayala A, Ro JY Tags: Ann Clin Lab Sci Source Type: research

The First Korean Case of Baraitser-Winter Cerebro-Fronto-Facial Syndrome with a Novel Mutation in ACTB Diagnosed Via Targeted Gene Panel Sequencing and Literature Review.
We report a two-year-old girl who had distinctive facial features, including hypertelorism, arched eyebrows, bilateral ptosis, short broad nose with a flat nasal tip, long philtrum, retrognathia, low-set ears, and a thin upper lip. In addition, she also exhibited short stature, pectus excavatum, developmental delay, brain malformation, and hearing loss. Targeted gene panel sequencing identified a de novo heterozygous missense variant c.826G>A (p.Glu276Lys) in ACTB This is the first Korean case of BWCFF with a novel mutation in ACTB. PMID: 33334799 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - November 1, 2020 Category: Laboratory Medicine Authors: Choi GJ, Kim MS, Park H, Kim JY, Choi JM, Lee SM, Jang JH, Cho SY, Jin DK Tags: Ann Clin Lab Sci Source Type: research

Thymic Carcinoid as the First Manifestation of Multiple Endocrine Neoplasia Type 1 Syndrome: A Case Report and Review of the Literature.
In this report, a 40-year-old male was diagnosed as MEN1 syndrome with thymic carcinoid, pancreatic cancer, hyperparathyroidism, and insulinoma with intrahepatic metastasis. Genetic testing showed the mutations of the MEN1 (c.378 G>A, p. Trp126*) in the patient, his children and two sisters, and GCKR (c.151C>T, p. Arg51*) gene in the patient and his children. The pathological examination showed that neuroendocrine tumor (NET) of the pancreas was characterized as 6 mitoses per 10 high-power fields (HPF), infiltration of adipose tissue, no intravascular tumor thrombus and nerve infiltration. In addition, NET of the liv...
Source: Annals of Clinical and Laboratory Science - November 1, 2020 Category: Laboratory Medicine Authors: Zheng M, Chen S, Qi C, Ren L, Zhen Y, Yu X, Wei L Tags: Ann Clin Lab Sci Source Type: research

Prostate Metastasis of Rare Lung Adenocarcinoma: A Case Report and Literature Review.
PMID: 33334801 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - November 1, 2020 Category: Laboratory Medicine Authors: Limin H, Wang K, Mao W, Yin L, Li W, Peng B Tags: Ann Clin Lab Sci Source Type: research

Commentary: Pharmacologic Correlates that Address the Clinicopathobiology of COVID-19.
Abstract COVID-19 could remain a pandemic until we get a SARS-CoV-2 vaccine to immunize the world population. In the interim, we have the opportunity to consider its clinicopathobiology in the context of the pharmacologic correlates available from the existing medical literature to prevent the COVID-19 infected patient from progressing into a fatal stage. This commentary serves as a forum for that end and suggests relatively non-toxic therapies that could be applied in a combinatorial fashion for consideration by the physicians and their patients with COVID-19 infection. PMID: 33334802 [PubMed - in process] (...
Source: Annals of Clinical and Laboratory Science - November 1, 2020 Category: Laboratory Medicine Authors: Brown RE, Tahseen D Tags: Ann Clin Lab Sci Source Type: research

Technical Note: Comparative Study of Three Different Fecal Calprotectin Immunoassays: TriCAL Study.
CONCLUSIONS: The CALiaGold® test showed good performance for FC assessment. Better correlation and agreement between immunoturbidimetric assays were confirmed. PMID: 33334803 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - November 1, 2020 Category: Laboratory Medicine Authors: Mérida FJ, Bel N, Sánchez-Montes S, Perez MJ, Perez V Tags: Ann Clin Lab Sci Source Type: research

Communication: The Follow-Up of Biomarkers Better Predicts the Poor Outcome in COVID-19 Patients.
In this study, 132 COVID-19 patients were classified as good outcome (n=62) and poor outcome (n=70) groups. Laboratory parameters were evaluated on admission and within 5-7 days after hospitalization. RESULTS: Baseline levels of neutrophil-lymphocyte ratio, CRP, procalcitonin, ferritin, D-dimer and LDH were higher (p
Source: Annals of Clinical and Laboratory Science - November 1, 2020 Category: Laboratory Medicine Authors: Basoglu OK, Ozhan MH, Ekren PK, Ak G, Tasbakan MS, Sayiner A Tags: Ann Clin Lab Sci Source Type: research

Communication: Correlation of Point of Care SARS-CoV-2 Serological Assay with Quantitative ELISA.
Abstract Due to the COVID-19 pandemic, the Food and Drug Administration issued an Emergency Use Authorization to permit developers of certain serological tests to market their product prior to a comprehensive review. Nonetheless, the reliability of these assays is of great importance in order to be useful as a tool in estimating the relative proportions of different populations that have been exposed to SARS-CoV-2. We provide a sampling of 145 individuals from an ambulatory setting simultaneously tested with a qualitative point of care rapid finger prick Lateral Wave® IgM and IgG assay and a sample for the May...
Source: Annals of Clinical and Laboratory Science - November 1, 2020 Category: Laboratory Medicine Authors: Lempel M, Lempel A, Bielory L Tags: Ann Clin Lab Sci Source Type: research

Chemical Pathology of Homocysteine VIII. Effects of Tocotrienol, Geranylgeraniol, and Squalene on Thioretinaco Ozonide, Mitochondrial Permeability, and Oxidative Phosphorylation in Arteriosclerosis, Cancer, Neurodegeneration and Aging.
Abstract A century ago a fat-soluble vitamin from leafy vegetables, later named vitamin E, was discovered to enhance fertility in animals. Vitamin E consists of 8 isomers of tocopherols and tocotrienols, each containing chromanol groups that confer antioxidant properties and differ only in the 15-carbon saturated phytyl poly-isoprenoid side chain of tocopherols and the 15-carbon unsaturated farnesyl poly-isoprenoid side chain of tocotrienols. Although tocotrienol was first isolated from rubber plants in 1964, its importance in multiple disease processes was not recognized until two decades later, when the choleste...
Source: Annals of Clinical and Laboratory Science - September 1, 2020 Category: Laboratory Medicine Authors: McCully KS Tags: Ann Clin Lab Sci Source Type: research

Hepatocyte Growth Factor Inhibits Renal Angiotensin II Expression in 5/6 Nephrectomized Rats.
This study aimed to investigate the effect of HGF on Ang II expression in CKD. A rat model of CKD was established using female Wistar rats subjected to 5/6 nephrectomy (5/6 Nx). HGF was overexpressed in rat renal tissues using PCI-neo-HGF. Immunohistochemical staining and western blot analysis of renal Ang II expression were performed in 5/6 Nx rats treated with vehicle (negative control), Lotensin (positive control), or HGF for different periods of time (before 5/6 Nx, 5 and 9 weeks after 5/6 Nx). Compared with the 0-week group (before 5/6 Nx group), the protein expression of Ang II was significantly induced in rat renal ...
Source: Annals of Clinical and Laboratory Science - September 1, 2020 Category: Laboratory Medicine Authors: Li C, Xiao Q, Gu C, Zhao Y, Liu L, Wang H Tags: Ann Clin Lab Sci Source Type: research

P-Gp and TOPO II Expression and Their Clinical Significance in Colon Cancer.
Abstract OBJECTIVE: Analysis of P-Gp and TOPO II expression levels and their clinical significance in colon cancer. METHODS: A total of 300 cases of paraffin embedded specimens of primary colon cancer were collected from the Affiliated Hospital of Putian University. The levels of P-Gp and TOPO II expression in colon cancer tissues were detected by the two-step En Vison. RESULTS: P-glycoprotein (P-Gp) was mainly localized in the membrane and nucleus of colon cancer cells and the positive expression rate was 62% (186/300). The positive expression of P-Gp correlated with gender, invasion depth and lymph nod...
Source: Annals of Clinical and Laboratory Science - September 1, 2020 Category: Laboratory Medicine Authors: Dang Y, Liu F, Zhao Y Tags: Ann Clin Lab Sci Source Type: research

Monitoring Treatment-Free Remission by Droplet Digital PCR in CML Patients with Deep Molecular Response to Tyrosine Kinase Inhibitor: An Analysis Based on Real-World Data.
This study aimed to compare and evaluate the clinical value of dd-PCR in predicting relapse in CML patients entering TFR. Using dd-PCR and RT-qPCR technology, dynamic BCR/ABL transcripts were detected in 13 CML patients who discontinued TKI treatment after sustaining undetectable BCR-ABL levels for a median time of 25 months. The results showed that in 13 patients, only 2 cases (22.2%) of 9 patients who executed planned discontinuation achieved TFR within 12 months. In the first 6 months, the detection rate of BCR/ABL transcripts by dd-PCR was higher than that by RT-qPCR and the two methods kept a positive correlation (r=0...
Source: Annals of Clinical and Laboratory Science - September 1, 2020 Category: Laboratory Medicine Authors: Zhu G, Yang Y, Wang H, Xie J, Hu J, Guo W, Zhang L, Liu Z, Chen X, Chang J, Xu J, Tan Y Tags: Ann Clin Lab Sci Source Type: research

Identification of Significant Genes in HIV/TB via Bioinformatics Analysis.
CONCLUSIONS: Twelve significantly up-regulated DEGs that may be potential therapeutic targets for HIV/TB were identified using a series of bioinformatics analytical methods. PMID: 33067206 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - September 1, 2020 Category: Laboratory Medicine Authors: Li X, Xie Y, Zhang D, Wang Y, Wu J, Sun X, Wu Q Tags: Ann Clin Lab Sci Source Type: research

Anti-Cancer Tumor Cell Necrosis of Epithelial Ovarian Cancer Cell Lines Depends on High Expression of HDM-2 Protein in Their Membranes.
CONCLUSIONS: Our results suggest that HDM-2 is highly expressed in the membranes of these ovarian cancer cell lines and colocalizes with PNC-27. We therefore conclude that the association of PNC-27 with preferentially expressed membrane HDM-2 isoforms results in the proposed model for the formation of transmembrane pores and epithelial ovarian cancer tumor cell necrosis, as previously described in a number of solid tissue and hematologic malignancies. PMID: 33067207 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - September 1, 2020 Category: Laboratory Medicine Authors: Thadi A, Gleeson EM, Khalili M, Shaikh MF, Goldstein E, Morano WF, Daniels LM, Grandhi N, Glatthorn H, Richard SD, Campbell PM, Sarafraz-Yazdi E, Pincus MR, Bowne WB Tags: Ann Clin Lab Sci Source Type: research

Genetic Diagnosis of Dravet Syndrome Using Next Generation Sequencing-Based Epilepsy Gene Panel Testing.
Abstract Dravet syndrome, one of the epileptic encephalopathies of childhood, is a genetic epilepsy caused by SCN1A mutation in 70-80% of the cases. Other genetic variants have been revealed in SCN1A-negative patients with Dravet syndrome. We investigated the utility of targeted gene panel testing in patients with Dravet syndrome and delineated the genotype-phenotype correlation. Targeted epilepsy gene panel testing including 40 genes was performed in 24 patients clinically diagnosed with Dravet syndrome. Detected variants were classified according to the guidelines of American College of Medical Genetics and Geno...
Source: Annals of Clinical and Laboratory Science - September 1, 2020 Category: Laboratory Medicine Authors: Lee J, Lee C, Park WY, Lee J Tags: Ann Clin Lab Sci Source Type: research

Tspan8 Is Highly Expressed in Clear Cell Renal Cell Carcinoma and Indicates Poor Prognosis.
The objective of the present study was to assess the expression of Tspan8 and its correlation with clinicopathological features in ccRCC. METHODS: Tspan8 expression was detected in 150 cases of ccRCC and matched paracancerous tissues by immunohistochemistry (IHC) and its relevance with prognosis was analyzed. RESULTS: Our data showed that the high-expression rate of Tspan8 in ccRCC tissues was 74.0%, which was significantly higher than those in paracancerous kidney tissues (43.3%, P=0.001). Meanwhile, Tspan8 expression was positively correlated with tumor size and WHO/ISUP grade in ccRCC. Significantly, Kaplan-Me...
Source: Annals of Clinical and Laboratory Science - September 1, 2020 Category: Laboratory Medicine Authors: Tang Y, Xie J, Chen Y, Zhang T, Zhuo H, Chen J, Yang P, Cui Q, Zhao L, Li L Tags: Ann Clin Lab Sci Source Type: research

The Prognostic Significance of TERT Locus Polymorphism (rs36115365) in Surgically Resected Non-Small Cell Lung Cancer.
CONCLUSIONS: These findings suggest that rs36115365 may contribute to the progression of NSCLC. PMID: 33067210 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - September 1, 2020 Category: Laboratory Medicine Authors: Lee DH, Jung SJ, Park WJ, Heo YR, Lee JH Tags: Ann Clin Lab Sci Source Type: research

The Clinical Significance of sICAM-1 in Differentiating Benign Breast Lesions from Breast Cancer.
Abstract OBJECTIVE: Breast cancer is a common type of malignant tumour worldwide and the second leading cause of death in women. The present study aims to investigate the clinical significance of serum soluble intercellular adhesion molecule-1 (sICAM-1) in differentiating benign breast lesions from breast cancer. METHODS: Plasma samples were obtained from 200 breast cancer patients, 47 patients with benign breast lesions and 50 age- and sex-matched healthy individuals as controls. Plasma levels of sICAM-1 were measured in all the samples using commercially available enzyme-linked immune-sorbent assay (ELISA) ...
Source: Annals of Clinical and Laboratory Science - September 1, 2020 Category: Laboratory Medicine Authors: Chen C, Chen Q, Dong Y, Liu X Tags: Ann Clin Lab Sci Source Type: research

Candida parapsilosis Infection: A Multilocus Microsatellite Genotyping-Based Survey Demonstrating an Outbreak in Hospitalized Patients.
nco A Abstract Microsatellite analysis identifies specific genotypes and the genetic relationship between strains. Our objective was to analyze the genotypes of C. parapsilosis strains isolated on different wards of a Tertiary-Referral University Center. We evaluated 70 C. parapsilosis strains in total, isolated from samples of patients admitted to five different wards over two years (January 2015-December 2016). Eight microsatellite markers were selected, and two multiplex PCR assays were set up for microsatellite analysis. The 70 strains, examined at eight microsatellite loci, showed 46 different multilocus geno...
Source: Annals of Clinical and Laboratory Science - September 1, 2020 Category: Laboratory Medicine Authors: Calà C, Fontana I, Di Carlo P, Mascarella C, Fasciana T, Reale S, Sergi C, Giammanco A Tags: Ann Clin Lab Sci Source Type: research

Microscopic Review of Nasopharyngeal Swabs as a Means of Benchside Quality Assurance.
CONCLUSION: We posit that samples without identifiable CCEs have a greater likelihood of inadequate collection. The basic, benchside protocol that we describe here demonstrates potential to reduce unnecessary re-testing when deployed to confirm negative tests despite high clinical suspicion: a strategy which may help conserve NPS reagents. PMID: 33067213 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - September 1, 2020 Category: Laboratory Medicine Authors: Matelski A, Siddique N, Mead P, Raimondi T, Jorgenson LC, Calderon O, Harman K, Farrell JJ Tags: Ann Clin Lab Sci Source Type: research

Enhancing the Role of the Medical Technologist in Serum Protein Electrophoresis Interpretation: A Pattern Recognition Approach to Identifying Obvious and Potentially Significant Subtle Alterations.
Abstract Medical technologists who perform serum protein electrophoresis can provide valuable input and assistance in the interpretation of the electrophoretic pattern. Recognition of common alterations in conjunction with the utilization of standardized interpretation comments expedites the final interpretation performed by the clinical pathologist. Improved understanding of the clinical utility of the electrophoretic interpretation enhances the role and satisfaction of the medical technologists. PMID: 33067214 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - September 1, 2020 Category: Laboratory Medicine Authors: Strobel SL, Lunsford KL Tags: Ann Clin Lab Sci Source Type: research

A Novel External Quality Assessment of Chromosomal Karyotype Analysis Based on Chimera Image Assembly.
CONCLUSION: The quality of chromosome karyotype analysis can be comprehensively evaluated by a new EQA based on assembly A1 or B1. PMID: 33067215 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - September 1, 2020 Category: Laboratory Medicine Authors: Weng BH, Shen XY, Xu YL, Ying J, Xu W, Zou DB, He LM, Jin K, Li LJ Tags: Ann Clin Lab Sci Source Type: research

Use of Serum Albumin Level as a Predictive Marker of Clinical Outcomes for Active Tuberculosis.
CONCLUSIONS: Our data demonstrates that serum albumin level changes during early anti-TB treatment are useful indicators for identifying TB patients at high-risk for treatment failure. PMID: 33067216 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - September 1, 2020 Category: Laboratory Medicine Authors: Liu R, Shu W, Song Y, Liu Y, Ma L, Gao M Tags: Ann Clin Lab Sci Source Type: research

A Novel Missense Mutation (L44V) of PAX2 Associated with Adult-Onset End-Stage Renal Disease and No Syndromic Features.
We report a novel PAX2 mutation identified by exome sequencing in a family with adult ESRD in the absence of other congenital syndromic features. PMID: 33067217 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - September 1, 2020 Category: Laboratory Medicine Authors: Oh SH, Keum C, Her M, Chung WY, Kim YH, Kim T Tags: Ann Clin Lab Sci Source Type: research

The First Korean Family with Aarskog-Scott Syndrome Harboring a Novel Mutation in FGD1 Diagnosed via Targeted Gene Panel Sequencing.
We report the clinical and molecular analysis of a family with ASS. A 31-month-old boy and his cousin were initially mistaken for having Noonan syndrome owing to short stature and facial dysmorphism. Considering the family history, we suspected the possibility of an X-linked genetic disease and performed targeted gene panel sequencing; a novel hemizygous variant c.1192-1 G>A in FGD1 was identified in both the proband and his cousin. This is the first report of ASS in Korea. Targeted gene panel sequencing can be an effective tool for diagnosing rare complex syndromes, including ASS. PMID: 33067218 [PubMed - in proce...
Source: Annals of Clinical and Laboratory Science - September 1, 2020 Category: Laboratory Medicine Authors: Bae GY, Kim MS, Kim JY, Jang JH, Lee SM, Cho SY, Jin DK Tags: Ann Clin Lab Sci Source Type: research

"Safety Measures" Do Not Provide Equivalent Safety for Extended Storage Platelets.
"Safety Measures" Do Not Provide Equivalent Safety for Extended Storage Platelets. Ann Clin Lab Sci. 2020 Sep;50(5):701-702 Authors: Mintz PD, Vallejo RP PMID: 33067220 [PubMed - in process] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - September 1, 2020 Category: Laboratory Medicine Authors: Mintz PD, Vallejo RP Tags: Ann Clin Lab Sci Source Type: research

Morphoproteomics Identifies the Foamy Alveolar Macrophage as an M2 Phenotype with PD-L1 Expression in the Early Lesion of Post-Primary Tuberculosis: Implications for Host Immune Surveillance and Therapy.
Abstract Post-primary tuberculosis (TB) disease is characterized by paucibacillary necrosis of the early lesion, tuberculous pneumonia, in the adult human lung. The mechanism is speculated to be a strong localized delayed type hypersensitive response (DTH). However, up to this date, no one has been able to identify the source of the large accumulation of MTB antigens required for the DTH response. Although it is known and accepted that the pathogen, Mycobacterium tuberculosis (MTB), significantly affects macrophage function and activity, few studies have focused on macrophages at the site of the early lesion of de...
Source: Annals of Clinical and Laboratory Science - June 30, 2020 Category: Laboratory Medicine Authors: Hwang SA, Ali Y, Fedotova E, Hunter RL, Brown RE Tags: Ann Clin Lab Sci Source Type: research

Correlation Research of Thymidine Synthase Expression in Circulating Tumor Cell and Clinical Characteristics of Colon Cancer.
CONCLUSIONS: CTC positive rate is a promising index for the diagnosis of colon cancer. The lack of TYMS in CTC makes EMT cells prone to becoming epithelial-like cells, and TYMS silence in CTC indicates that the tumor is in the left colon. PMID: 32826238 [PubMed - as supplied by publisher] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - June 30, 2020 Category: Laboratory Medicine Authors: Jiang Y, Liu K, Shi Y, Li Y, Ji X, Zhao R Tags: Ann Clin Lab Sci Source Type: research

Aquaporin-5 Expression Is Reduced in Lymphocytic Colitis.
CONCLUSIONS: Colorectal AQP5 expression is reduced in most cases of LC. Markedly reduced AQP5 expression in LC may identify a subset of patients with suboptimal response to enteric steroid treatment. Additional larger studies are needed to confirm these findings.This abstract was presented in part at Digestive Diseases Week in San Diego, CA, May 2019. PMID: 32826239 [PubMed - as supplied by publisher] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - June 30, 2020 Category: Laboratory Medicine Authors: Tong YT, Dupont AW, Cash BD, Ertan A, Younes M Tags: Ann Clin Lab Sci Source Type: research

Decreased Lymphocyte to Monocyte Ratio and Increased Neutrophil to Lymphocyte Ratio Observed in Rotavirus-Positive Acute Gastroenteritis in Children: A Retrospective Study.
CONCLUSIONS: Decreased LYM and MPV and increased NEU and MON were observed in RPAG. Low LMR and high NLR may indicate HRV infection in children with acute gastroenteritis and, therefore, can be useful markers for the diagnosis and differential diagnosis of HRV infection in low resource environments, thereby preventing overuse of antibiotics and antiviral. PMID: 32826240 [PubMed - as supplied by publisher] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - June 30, 2020 Category: Laboratory Medicine Authors: Zhang C, Li G, Zhang H, Zhang H, Fei Y Tags: Ann Clin Lab Sci Source Type: research

High Fibrinogen and Platelets Correlate with Poor Survival in Gastric Cancer Patients.
Abstract OBJECTIVE: Gastric cancer (GC) is one of the most common malignant tumors in the world, and its incidence rate ranks the fourth in the world. Hyperfibrinogenemia and hyperthrombocytopaemia are often associated with malignancy and poor prognosis. The purpose of this study was to explore the relationship between high levels of fibrinogen and platelets and the clinicopathologic features and overall survival (OS) of gastric cancer. METHODS: We enrolled a total of 341 gastric cancer patients from our hospital between January 2014 and January 2015. GC patients were retrospectively assessed using a Kaplan-M...
Source: Annals of Clinical and Laboratory Science - June 30, 2020 Category: Laboratory Medicine Authors: Zhang Y, Liu N, Liu C, Cao B, Zhou P, Yang B Tags: Ann Clin Lab Sci Source Type: research

Clinical Significance of microRNA-146a in Patients with Ulcerative Colitis.
CONCLUSIONS: miRNA-146a was down-regulated in UC patients and was negatively correlated with serum levels of inflammatory factors as well as severity of UC patients. PMID: 32826242 [PubMed - as supplied by publisher] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - June 30, 2020 Category: Laboratory Medicine Authors: Feng J, Zhu Y, Chen L, Wang M Tags: Ann Clin Lab Sci Source Type: research

Efficient Definitive Endoderm Differentiation from Human Parthenogenetic Embryonic Stem Cells Induced by Activin A and Wnt3a.
CONCLUSIONS: Through the combination treatment using activin A and Wnt3a, DE differentiation from hPESCs culminated at 48 h, which can be regarded as the optimal time-point to induce differentiation of endodermal cells such as pancreatic, liver, and intestinal cells. PMID: 32826243 [PubMed - as supplied by publisher] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - June 30, 2020 Category: Laboratory Medicine Authors: Liang R, Xiao X, Luo L, Chen T, Yang H, Wang W, Zhang Y, Wang Z Tags: Ann Clin Lab Sci Source Type: research

TNF Inhibitor Pomalidomide Sensitizes Glioblastoma Cells to EGFR Inhibition.
CONCLUSIONS: We demonstrated that GBM resistance to EGFR inhibition was mediated by the activation of TNF. The combination of EGFR inhibitor and TNF inhibitor may have potential clinical implication in treating patients with EGFR-positive GBM. PMID: 32826244 [PubMed - as supplied by publisher] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - June 30, 2020 Category: Laboratory Medicine Authors: Luo Z, Wang B, Liu H, Shi L Tags: Ann Clin Lab Sci Source Type: research

Distinct Prognostic Values of the mRNA Expression of Glucose Transporters in Non-Small Cell Lung Cancer.
In conclusion, our study provides new insights into the prognostic values of GLUT members in LUAD, but the molecular mechanisms by which GLUTs contribute to disease aggression and the different functional roles of GLUT members need further study. PMID: 32826245 [PubMed - as supplied by publisher] (Source: Annals of Clinical and Laboratory Science)
Source: Annals of Clinical and Laboratory Science - June 30, 2020 Category: Laboratory Medicine Authors: Du H, Liu Y, Yuan Y, Zhang Y, Geng H Tags: Ann Clin Lab Sci Source Type: research

Correlation Between the VerifyNow P2Y12 Assay and the Newly Developed APAL System in Neuroendovascular Patients.
Abstract OBJECTIVE: Light transmission aggregometry (LTA) is the gold standard method for assessing platelet function. Recently, a new parameter called adenosine diphosphate (ADP)-induced platelet aggregation level (APAL) was developed to aid interpretation of LTA results. APAL is a score calculated based on platelet aggregation patterns upon exposure to 1 μM and 10 μM ADP and is determined using an automated coagulation analyzer. We compared APAL and VerifyNow P2Y12 assay for neuroendovascular patients. METHODS: 42 patients who have received antiplatelet therapy were studied. Platelet function tests we...
Source: Annals of Clinical and Laboratory Science - June 30, 2020 Category: Laboratory Medicine Authors: Uematsu K, Sakayori T, Ishida H, Omori Y, Kitano K, Matsubara H, Enomoto Y Tags: Ann Clin Lab Sci Source Type: research