Filtered By:
Education: Learning

This page shows you your search results in order of date. This is page number 7.

Order by Relevance | Date

Total 122 results found since Jan 2013.

Novel compound from Polygonum multiflorum inhibits inflammatory response in LPS-stimulated microglia by upregulating AMPK/ Nrf2 pathways
Publication date: Available online 19 August 2016 Source:Neurochemistry International Author(s): Sun Young Park, Mei Ling Jin, Seon Yeong Chae, Min Jung Ko, Yung Hyun Choi, Geuntae Park, Young-Whan Choi Polygonum multiflorum extracts are known to improve memory and learning ability, and have neuroprotective and anti-aging activity. However, its function and the underlying mechanisms in neuroinflammation-mediated neurodegenerative disease remain poorly understood. In the present study, we investigated the anti-neuroinflammatory effects of several compounds from P. multiflorum, and found a novel compound, CRPE55IB. The CRPE...
Source: Neurochemistry International - August 19, 2016 Category: Neuroscience Source Type: research

Modeling cis-regulation with a compendium of genome-wide histone H3K27ac profiles METHOD
This study demonstrates the advantage of integrating a large compendium of historical epigenetic data for genomic studies of transcriptional regulation.
Source: Genome Research - July 26, 2016 Category: Genetics & Stem Cells Authors: Wang, S., Zang, C., Xiao, T., Fan, J., Mei, S., Qin, Q., Wu, Q., Li, X. L., Xu, K., He, H. H., Brown, M., Meyer, C. A., Liu, X. S. Tags: METHOD Source Type: research

Ranking novel cancer driving synthetic lethal gene pairs using TCGA data.
In this study, we propose an efficient and comprehensive in-silico pipeline to rank novel SL gene pairs by mining vast amounts of accumulated tumor high-throughput sequencing data in The Cancer Genome Atlas (TCGA), coupled with other protein interaction networks and cell line information. Our pipeline integrates three significant features, including mutation coverage in TCGA, driver mutation probability and the quantified cancer network information centrality, into a ranking model for SL gene pair identification, which is presented as the first learning-based method for SL identification. As a result, 107 potential SL gene...
Source: Oncotarget - July 21, 2016 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Dissociable roles for histone acetyltransferase p300 and PCAF in hippocampus and perirhinal cortex‐mediated object memory
Abstract The importance of histone acetylation for certain types of memory is now well established. However, the specific contributions of the various histone acetyltransferases to distinct memory functions remain to be determined; therefore, we employed selective histone acetyltransferase protein inhibitors and short‐interference RNAs to evaluate the roles of CREB‐binding protein (CBP), E1A‐binding protein (p300) and p300/CBP‐associated factor (PCAF), in hippocampus and perirhinal cortex‐mediated object memory. Rats were tested for short‐ and long‐term memory in the object‐in‐place task, a task which rel...
Source: Genes, Brain and Behavior - May 31, 2016 Category: Genetics & Stem Cells Authors: Krista A. Mitchnick, Samantha D. Creighton, Jacob M. Cloke, Michael Wolter, Oleksiy Zaika, Bethany Christen, Monika Van Tiggelen, Bettina E. Kalisch, Boyer D. Winters Tags: Original Article Source Type: research

GSE72534 Modeling genome-wide transcriptional cis-regulation in n LNCaP-abl cell line after siRNA knock down of a series of gene factors RNA-seq
We describe, MARGE, Model-based Analysis of the Regulation of Gene Expression, a robust methodology that leverages a large library of genome-wide H3K27ac ChIP-seq profiles to predict key regulated genes and cis-regulatory regions in human or mouse. MARGE adopts a gene centric approach to define a regulatory potential that summarizes the aggregate activity of multiple cis-regulatory elements on each gene. This model is effective in describing cis-regulatory activity and, unlike the super-enhancer based approach, is highly predictive of gene expression changes in response to BET-bromodomain inhibitors. We show that linear co...
Source: GEO: Gene Expression Omnibus - May 16, 2016 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

BOD1 Is Required for Cognitive Function in Humans and Drosophila
We report that fibroblast cell lines derived from homozygous BOD1 mutation carriers show aberrant localisation of the cell cycle kinase PLK1 and its phosphatase PP2A at mitotic kinetochores. However, in contrast to the mitotic arrest observed in BOD1-siRNA treated HeLa cells, patient-derived cells progressed through mitosis with no apparent segregation defects but at an accelerated rate compared to controls. The relatively normal cell cycle progression observed in cultured cells is in line with the absence of gross structural brain abnormalities in the affected individuals. Moreover, we found that in normal adult brain tis...
Source: PLoS Genetics - May 10, 2016 Category: Genetics & Stem Cells Authors: Sahar Esmaeeli-Nieh Source Type: research

Lipoxin A4 Reduces Inflammation Through Formyl Peptide Receptor 2/p38 MAPK Signaling Pathway in Subarachnoid Hemorrhage Rats Basic Sciences
Conclusions— Exogenous LXA4 inhibited inflammation by activating FPR2 and inhibiting p38 after SAH. LXA4 may serve as an alternative treatment to relieve early brain injury after SAH.
Source: Stroke - January 25, 2016 Category: Neurology Authors: Guo, Z., Hu, Q., Xu, L., Guo, Z.-N., Ou, Y., He, Y., Yin, C., Sun, X., Tang, J., Zhang, J. H. Tags: Animal Models of Human Disease Basic Sciences Source Type: research

Low-Molecular-Weight Fucoidan Attenuates Mitochondrial Dysfunction and Improves Neurological Outcome After Traumatic Brain Injury in Aged Mice: Involvement of Sirt3.
Abstract Traumatic brain injury (TBI) is a leading cause of death and long-term disability. Fucoidan, a sulfated polysaccharide extracted from brown algae, possesses potent anti-oxidative and anti-inflammatory effects. Considering TBI happens frequently in adults, especially in aged individuals, we herein sought to define the protective effects of low-molecular-weight fucoidan (LMWF) in the aged mice. 16- to 18-month-old mice administered with LMWF (1-50 mg/kg) or vehicle were subjected to TBI using a controlled cortical impact (CCI) model. LMWF at the doses of 10 and 50 mg/kg significantly reduced both cortical...
Source: Cellular and Molecular Neurobiology - January 7, 2016 Category: Cytology Authors: Wang T, Zhu M, He ZZ Tags: Cell Mol Neurobiol Source Type: research

Upregulating the Expression of Survivin-HBXIP Complex Contributes to the Protective Role of IMM-H004 in Transient Global Cerebral Ischemia/Reperfusion
Abstract IMM-H004, a 3-piperazinylcoumarin compound derived from coumarin, has been proved effective against CA1 cell loss and spatial learning impairments resulting from transient global ischemia/reperfusion (TGCI/R), while the mechanism is still largely unknown. Here, we confirmed that treatment of rats with IMM-H004 immediately after TGCI/R ameliorated delayed neuronal death (DND) in the CA1 of hippocampus and cortex. Further study suggested that IMM-H004 contributed to the expression of antiapoptotic protein survivin through the activation of PI3K-dependent protein kinase B (PKB/Akt), which led to the phosphor...
Source: Molecular Neurobiology - January 7, 2016 Category: Neurology Source Type: research

Helios & reg; Gene Gun-Mediated Transfection of the Inner Ear Sensory Epithelium: Recent Updates
The transfection of vertebrate inner ear hair cells has proven to be challenging. Therefore, many laboratories attempt to use and improve different transfection methods. Each method has its own advantages and disadvantages. A particular researcher & rsquo;s skills in addition to available equipment and the type of experiment (in vivo or in vitro) likely determine the transfection method of choice. Biolistic delivery of exogenous DNA, mRNA, or siRNA, also known as Helios & reg; Gene Gun-mediated transfection, uses the mechanical energy of compressed helium gas to bombard tissue with micron- or submicron-sized DNA or RNA-coa...
Source: Springer protocols feed by Molecular Medicine - December 31, 2015 Category: Molecular Biology Source Type: news

Helios® Gene Gun-Mediated Transfection of the Inner Ear Sensory Epithelium: Recent Updates
The transfection of vertebrate inner ear hair cells has proven to be challenging. Therefore, many laboratories attempt to use and improve different transfection methods. Each method has its own advantages and disadvantages. A particular researcher’s skills in addition to available equipment and the type of experiment (in vivo or in vitro) likely determine the transfection method of choice. Biolistic delivery of exogenous DNA, mRNA, or siRNA, also known as Helios® Gene Gun-mediated transfection, uses the mechanical energy of compressed helium gas to bombard tissue with micron- or submicron-sized DNA or RNA-coated ...
Source: Springer protocols feed by Molecular Medicine - December 31, 2015 Category: Molecular Biology Source Type: news

Beta-arrestin1 and 2 differently modulate metabotropic glutamate receptor 7 signaling in rat developmental sevoflurane-induced neuronal apoptosis
This study also reveals a β-arr-biased agonism at GPCRs (e.g. mGluR7).
Source: Neuroscience - December 11, 2015 Category: Neuroscience Source Type: research

Functional genome-wide siRNA screen identifies KIAA0586 as mutated in Joubert syndrome
Defective primary ciliogenesis or cilium stability forms the basis of human ciliopathies, including Joubert syndrome (JS), with defective cerebellar vermis development. We performed a high-content genome-wide small interfering RNA (siRNA) screen to identify genes regulating ciliogenesis as candidates for JS. We analyzed results with a supervised-learning approach, using SYSCILIA gold standard, Cildb3.0, a centriole siRNA screen and the GTex project, identifying 591 likely candidates. Intersection of this data with whole exome results from 145 individuals with unexplained JS identified six families with predominantly compou...
Source: eLife - June 23, 2015 Category: Biomedical Science Authors: Roosing, S., Hofree, M., Kim, S., Scott, E., Copeland, B., Romani, M., Silhavy, J. L., Rosti, R. O., Schroth, J., Mazza, T., Miccinilli, E., Zaki, M. S., Swoboda, K. J., Milisa-Drautz, J., Dobyns, W. B., Mikati, M. A., Incecik, F., Azam, M., Borgatti, R., Tags: Human biology and medicine Source Type: research

Bisphenol-A Mediated Inhibition of Hippocampal Neurogenesis Attenuated by Curcumin via Canonical Wnt Pathway
Abstract Bisphenol A (BPA) is an environmental xenoestrogenic endocrine disruptor, utilized for production of consumer products, and exerts adverse effects on the developing nervous system. Recently, we found that BPA impairs the finely tuned dynamic processes of neurogenesis (generation of new neurons) in the hippocampus of the developing rat brain. Curcumin is a natural polyphenolic compound, which provides neuroprotection against various environmental neurotoxicants and in the cellular and animal models of neurodegenerative disorders. Here, we have assessed the neuroprotective efficacy of curcumin against BPA-m...
Source: Molecular Neurobiology - May 12, 2015 Category: Neurology Source Type: research

Activation of α7 nicotinic acetylcholine receptors increases intracellular cAMP levels via activation of AC1 in hippocampal neurons.
Abstract The activation of α7 nAChRs has been shown to improve hippocampal-dependent learning and memory. However, the molecular mechanism of α7 nAChRs' action remains elusive. We previously reported that activation of α7 nAChRs induced a prolonged enhancement of glutamatergic synaptic transmission in a PKA-dependent manner. Here, we investigated any connection between the activation of the α7 nAChR and cAMP signaling in hippocampal neurons. To address this question, we employed a FRET-based biosensor to measure the intracellular cAMP levels directly via live cell imaging. We found that application of the α7 ...
Source: Neuropharmacology - April 29, 2015 Category: Drugs & Pharmacology Authors: Cheng Q, Yakel JL Tags: Neuropharmacology Source Type: research