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Total 122 results found since Jan 2013.

Neuromics' Transfection Kits-Genes Studied
Delivering siRNA, miRNA, Plasmids and Viral Vectors for Gene Expression Analysis.I have shared the many genes researchers have studied using our Transfection Kits. These include: β-arrestin, ABCA, ASIC, β-arrestin, CAV1.2, CX3CR1, DOR, EHDAC2, LOVL4, IKBKAP, K+-ATPase, KV1.1, KV9.1 , neuroligin 2, The β3 subunit of the Na+,K+-ATPase, NTS1, NAV1.8, NTS1, NOV, Raf-1, RANK, SNSR1, hTert, NOV, Survivin, TLR4, Troy and TRPV1 and More!We can now add GPNMB to this list: Lili Hou, Yanfeng Zhang, Yong Yang, Kai Xiang, Qindong Tan, Qulian Guo. Intrathecal siRNA Against GPNMB Attenuates Nociception in a Rat Model of Neuropath...
Source: siRNA and DsiRNA Transfection Efficiency - July 23, 2014 Category: Neuroscience Tags: Delivering siRNA to the CNS Gene Expression Analysis Gene Silencing i-Fect in vivo siRNA pn-Fect tansfection Transfection Kits Source Type: news

Neuromics ' Transfection Kits-Genes Studied
Delivering siRNA, miRNA, Plasmids and Viral Vectors  for Gene Expression Analysis . I have shared the many genes researchers have studied using our Transfection Kits . These include: β-arrestin, ABCA, ASIC, β-arrestin, CAV1.2, CX3CR1, DOR, EHDAC2, LOVL4, IKBKAP, K+-ATPase, KV1.1, KV9.1 , neuroligin 2, The β3 subunit of the Na+,K+-ATPase, NTS1, NAV1.8, NTS1, NOV, Raf-1, RANK, SNSR1, hTert, NOV, Survivin, TLR4, Troy and TRPV1 and More! We can now add GPNMB to this list: Lili Hou, Yanfeng Zhang, Yong Yang, Kai Xiang, Qindong Tan, Qulian Guo. Intrathecal siRNA Against GPNMB Attenuates Nociception in a Rat Model of N...
Source: siRNA and DsiRNA Transfection Efficiency - July 23, 2014 Category: Neuroscience Tags: Delivering siRNA to the CNS Gene Expression Analysis Gene Silencing i-Fect in vivo siRNA pn-Fect tansfection Transfection Kits Source Type: news

HDAC2 and Anxiety in Alcoholism
The Impact of HDAC2 Gene Expression on AnxietyOur i-Fect Transfection Kit continues to be a potent tool for testing the impact of altered gene expression on behavior. see: SACHIN MOONAT. The Role of Amygdaloid Chromatin and Synaptic Remodeling in Anxiety and Alcoholism. THESIS Submitted as partial fulfillment of the requirements for the degree of Doctor of Philosophy in Neuroscience in the Graduate College of the University of Illinois at Chicago, 2014.The author hypothesized that increased HDAC2 would have a positive impact on anxiety in alchohol preferring (P) rats. Specifically, HDAC2-induced histone modifications ...
Source: siRNA and DsiRNA Transfection Efficiency - November 24, 2014 Category: Neuroscience Tags: Alcoholism Anxiety BDNF Gene Expression Analysis Gene Silencing HDAC2 gene Learning Source Type: news

Pain Research Gene Expression Analysis
Potent and Proven Transfection KitsPain Researchers have successfully modulated 25+ genes involved in pain pathways using our Transfection Kits. Highlights include: DOR,The β3 subunit of  Na+,K+-ATPase, NTS1, NAV1.8, Kv 1.1, Kv 9.1, TROY, NOV, β-arrestin, TRPV1, CAV1.2, TLR4 and ASIC and more!  To learn more, check out our Transfection Kit Publications and Blog.Figures: Intrathecal Kv9.1 siRNA treatment induces pain behaviors in naive rats. A, qRT-PCR quantification of Kv9.1 mRNA in rat PASMC cultures transfected with one of three Kv9.1 siRNA sequences or control siRNA. B, qRT-PCR showing Kv9.1 in vivo knock-do...
Source: Neuromics - July 23, 2014 Category: Neuroscience Tags: chronic pain gene expression analysis Gene Silencing i-Fect inflammatory nociception Nociceptin Pain Research siRNA delivery in-vivo Source Type: news

In vivo evaluation and Alzheimer's disease treatment outcome of siRNA loaded dual targeting drug delivery system.
Abstract To deliver drugs to treat Alzheimer's disease (AD), nanoparticles should firstly penetrate through blood brain barrier, and then target neurons. Recently, we developed an Apo A-I and NL4 dual modified nanoparticle (ANNP) to deliver beta-amyloid converting enzyme 1 (BACE1) siRNA. Although promising in vitro results were obtained, the in vivo performance was not clear. Therefore, in this study, we further evaluated the in vivo neuroprotective effect and toxicity of the ANNP/siRNA. The ANNP/siRNA was 80.6 nm with good stability when incubated with serum. The ANNP/siRNA could target both bEnd.3 and PC12 cells...
Source: Current Pharmaceutical Biotechnology - February 4, 2019 Category: Biotechnology Authors: Zhang C, Shen L, Gu Z, Liu X, Lin H Tags: Curr Pharm Biotechnol Source Type: research

Genes, Vol. 13, Pages 319: The siRNA Off-Target Effect Is Determined by Base-Pairing Stabilities of Two Different Regions with Opposite Effects
In this study, we investigated the most responsible region for the off-target effect by conducting a comprehensive analysis of the thermodynamic properties of all possible siRNA subregions that involved a machine learning technique using a random sampling procedure. As a result, the thermodynamic stability of nucleotides 2–5 showed the highest positive correlation with the off-target effect, and nucleotides 8–14 showed the most negative correlation. Thus, it is revealed that the siRNA off-target effect is determined by the base-pairing stabilities of two different subregions with opposite effects.
Source: Genes - February 9, 2022 Category: Genetics & Stem Cells Authors: Yoshiaki Kobayashi Shen Tian Kumiko Ui-Tei Tags: Article Source Type: research

Nanovehicle-based small interfering RNA (siRNA) delivery for therapeutic purposes: a new molecular approach in the pharmacogenomics.
Abstract RNA interference (RNAi) is a process for regulating the gene expression in which small interfering RNAs (siRNAs) silence target genes. siRNA-based therapy as a new molecular treatment approach, offers therapeutic prospects for many common diseases such as cancer and cardiovascular disorders. Nevertheless, the efficacy of siRNA delivery has, so far, remained a challenging issue. This is due to their easy degradation through the circulation system and the difficulties in the intracellular delivery to specific tissues where they silence the target genes. There have been many efforts to develop suitable, safe...
Source: Current Clinical Pharmacology - July 9, 2018 Category: Drugs & Pharmacology Authors: Akhtari J, Tafazoli A, Mehrad-Majd H, Mahrooz A Tags: Curr Clin Pharmacol Source Type: research

Abstract 5323: Integrated computational cell-line modeling of drug sensitivity and high-throughput siRNA screening reveals novel molecular biomarkers for conventional chemotherapy
Conclusions: We present an integrated approach that combines a novel Bayesian multi-task learning model with high-throughput siRNA screens. Our approach aims to uncover sets of important aberrations and allows for the subtyping of drugs based on similarities in targets and mechanisms of action. We integrate our results with high-throughput RNAi experiments to identify synthetic lethal events in specific therapeutic context. Citation Format: Olga H. Nikolova, Mehmet Gönen, Rodrigo Dienstmann, In Sock Jang, Russell Moser, Silvia Cermelli, Chang Xu, Ryan M. Mitchell, Eduardo Mendez, Carla Grandori, Christopher Kemp, Stephen ...
Source: Cancer Research - September 30, 2014 Category: Cancer & Oncology Authors: Nikolova, O. H., Gonen, M., Dienstmann, R., Jang, I. S., Moser, R., Cermelli, S., Xu, C., Mitchell, R. M., Mendez, E., Grandori, C., Kemp, C., Friend, S., Guinney, J., Margolin, A. Tags: Molecular and Cellular Biology Source Type: research

Lipid-based siRNA Nanodelivery Systems: a Learning Process for Improving Transfer from Concepts to Clinical Applications.
CONCLUSION: Formulation design should be increasingly addressed with industrial criteria; it should be based on quality by design and on the estimation of critical attributes that affect performance, and supported by a range of characterization techniques and appropriate analytical methods. PMID: 30160217 [PubMed - as supplied by publisher]
Source: Current Clinical Pharmacology - August 29, 2018 Category: Drugs & Pharmacology Authors: Perez SE, Carlucci AM Tags: Curr Clin Pharmacol Source Type: research

Delivery of BACE1 siRNA mediated by TARBP-BTP fusion protein reduces β-amyloid deposits in a transgenic mouse model of Alzheimer's disease.
In this report, we further substantiate the approach through an extended use in AβPP-PS1 mice, which upon treatment with seven doses of β-secretase AβPP cleaving Enzyme 1 (BACE1) TARBP-BTP:siRNA, led to target-specific effect in the mouse brain. Concomitant gene silencing of BACE1, and consequent reduction in plaque load in the cerebral cortex and hippocampus (greater than 60%) in mice treated with TARBP-BTP:siRNA complex, led to improvement in spatial learning and memory. The study validates the efficiency of TARBP-BTP fusion protein as an efficient mediator of RNAi, giving considerable scope for future intervention in...
Source: Journal of Biosciences - February 28, 2019 Category: Biomedical Science Authors: Haroon MM, Saba K, Boddedda VH, Kumar JM, Patel AB, Gopal V Tags: J Biosci Source Type: research

Reconsideration of in silico siRNA design from a perspective of heterogeneous data integration: problems and solutions.
Abstract The success of RNA interference (RNAi) depends on the interaction between short interference RNAs (siRNAs) and mRNAs. Design of highly efficient and specific siRNAs has become a challenging issue in applications of RNAi. Here, we present a detailed survey on the state-of-the-art siRNAs design, focusing on several key issues with the current in silico RNAi studies, including: (i) inconsistencies among the proposed guidelines for siRNAs design and the incomplete list of siRNAs features, (ii) improper integration of the heterogeneous cross-platform siRNAs data, (iii) inadequate consideration of the binding s...
Source: Briefings in Bioinformatics - December 29, 2012 Category: Bioinformatics Authors: Liu Q, Zhou H, Zhu R, Xu Y, Cao Z Tags: Brief Bioinform Source Type: research

Functional genome-wide siRNA screen identifies KIAA0586 as mutated in Joubert syndrome
Defective primary ciliogenesis or cilium stability forms the basis of human ciliopathies, including Joubert syndrome (JS), with defective cerebellar vermis development. We performed a high-content genome-wide small interfering RNA (siRNA) screen to identify genes regulating ciliogenesis as candidates for JS. We analyzed results with a supervised-learning approach, using SYSCILIA gold standard, Cildb3.0, a centriole siRNA screen and the GTex project, identifying 591 likely candidates. Intersection of this data with whole exome results from 145 individuals with unexplained JS identified six families with predominantly compou...
Source: eLife - June 23, 2015 Category: Biomedical Science Authors: Roosing, S., Hofree, M., Kim, S., Scott, E., Copeland, B., Romani, M., Silhavy, J. L., Rosti, R. O., Schroth, J., Mazza, T., Miccinilli, E., Zaki, M. S., Swoboda, K. J., Milisa-Drautz, J., Dobyns, W. B., Mikati, M. A., Incecik, F., Azam, M., Borgatti, R., Tags: Human biology and medicine Source Type: research

A dual druggable genome-wide siRNA and compound library screening approach identifies modulators of parkin recruitment to mitochondria Molecular Bases of Disease
Genetic and biochemical evidence points to an association between mitochondrial dysfunction and Parkinson's disease (PD). PD-associated mutations in several genes have been identified and include those encoding PTEN-induced putative kinase 1 (PINK1) and parkin. To identify genes, pathways, and pharmacological targets that modulate the clearance of damaged or old mitochondria (mitophagy), here we developed a high-content imaging-based assay of parkin recruitment to mitochondria and screened both a druggable genome-wide siRNA library and a small neuroactive compound library. We used a multiparameter principal component analy...
Source: Journal of Biological Chemistry - March 5, 2020 Category: Chemistry Authors: Helen L. Scott, Nicola Buckner, Francesc Fernandez-Albert, Elisa Pedone, Lorena Postiglione, Gongyu Shi, Nicholas Allen, Liang-Fong Wong, Lorenzo Magini, Lucia Marucci, Gregory A. O'Sullivan, Sarah Cole, Justin Powell, Peter Maycox, James B. Uney Tags: Neurobiology Source Type: research

GSE136193 Characterizing epigenetic effects of HISTONE DEACETYLASE 6 on DNA methylation, siRNA biogenesis and nucleosome positioning using two hda6 alleles in ros1 background
Contributors : Shoudong Zhang ; Huan Zhong ; Qianwen WangSeries Type : Genome binding/occupancy profiling by high throughput sequencing ; Non-coding RNA profiling by high throughput sequencing ; Methylation profiling by high throughput sequencingOrganism : Arabidopsis thalianaDNA cytosine methylation is a hallmark of epigenetic gene silencing. DNA demethylation requires ROS1, a bifunctional  DNA glycosylase/lyases and open chromatin status mediated by IDM1. HDA6 is a RDP3-like histone deacetylase and was confirmed to mediate DNA methylation. In previous screening for ros1 suppressor, we identified two hda6 mutants revert...
Source: GEO: Gene Expression Omnibus - July 7, 2021 Category: Genetics & Stem Cells Tags: Genome binding/occupancy profiling by high throughput sequencing Non-coding RNA profiling by high throughput sequencing Methylation profiling by high throughput sequencing Arabidopsis thaliana Source Type: research

Exercise and Sodium Butyrate Transform a Subthreshold Learning Event into Long-Term Memory via a Brain-Derived Neurotrophic factor-Dependent Mechanism
& Carl W Cotman Keywords: hippocampus; learning & memory; molecular & Cellular Neurobiology; Neurogenetics; object location memory; plasticity; siRNA; subthreshold
Source: Neuropsychopharmacology - May 15, 2013 Category: Neurology Authors: Karlie A IntlekoferNicole C BerchtoldMelissa MalvaezAnthony J CarlosSusan C McQuownMichael J CunninghamMarcelo A WoodCarl W Cotman Tags: hippocampus learning & memory molecular Cellular Neurobiology Neurogenetics object location memory plasticity siRNA subthreshold Source Type: research