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Total 117 results found since Jan 2013.

Fundamental Studies on Development of Next-generation Medium Sized Peptide Drugs
Yakugaku Zasshi. 2022;142(10):1061-1066. doi: 10.1248/yakushi.22-00115.ABSTRACTMedium-sized peptides are expected as a next-generation drug discovery modality because they combine the properties of conventional small-molecule drugs and biopharmaceuticals. Nonetheless, peptides are easily degraded by digestive enzymes such as protease in the body, which could be problematic for the development of peptide-based drugs. To overcome such a problem, peptide-based foldamers containing non-proteinogenic amino acids or cyclized peptides have been reported. In addition, peptides must form stable secondary structures and their side c...
Source: Yakugaku Zasshi : Journal of the Pharmaceutical Society of Japan - October 2, 2022 Category: Drugs & Pharmacology Authors: Takashi Misawa Source Type: research

cbx2 is a functional target of the let-7 family in the gonad of Japanese flounder (Paralichthys olivaceus)
Comp Biochem Physiol B Biochem Mol Biol. 2022 Sep 22:110799. doi: 10.1016/j.cbpb.2022.110799. Online ahead of print.ABSTRACTAs a key member of the miRNA family, the role and target gene of the let-7 family in the gonad of Japanese flounder (Paralichthys olivaceus) is unclear. Chromobox homolog 2 (CBX2) is one of the core components of the polycomb group complex (PcG) and significantly influences gonadal development. The deletion of CBX2 can lead to sex reversal in mammals. Therefore, exploring the relationship between the let-7 family and cbx2 is crucial to clarify the role played by the let-7 family in the gonad of Japane...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - September 26, 2022 Category: Biochemistry Authors: Yahui Wu Yangyang Wang Yidong Xue Ningning Wu Jikui Wu Junling Zhang Source Type: research

Vutrisiran: First Approval
This article summarizes the milestones in the development of vutrisiran leading to this first approval in hATTR amyloi dosis.
Source: Drugs - August 23, 2022 Category: Drugs & Pharmacology Source Type: research

Human Guanylate-Binding Protein 1 Positively Regulates Japanese Encephalitis Virus Replication in an Interferon Gamma Primed Environment
RNA virus infection triggers interferon (IFN) receptor signaling, leading to the activation of hundreds of interferon-stimulated genes (ISGs). Guanylate-binding proteins (GBPs) belong to one such IFN inducible subfamily of guanosine triphosphatases (GTPases) that have been reported to exert broad anti-microbial activity and regulate host defenses against several intracellular pathogens. Here, we investigated the role of human GBP1 (hGBP1) in Japanese encephalitis virus (JEV) infection of HeLa cells in both an IFNγ unprimed and primed environment. We observed enhanced expression of GBP1 both at transcript and protein level...
Source: Frontiers in cellular and infection microbiology - May 19, 2022 Category: Microbiology Source Type: research

Complement C3 and Activated Fragment C3a Are Involved in Complement Activation and Anti-Bacterial Immunity
In this study, we examined the immunological properties of C3 and its activated fragment C3a from Japanese flounder (Paralichthys olivaceus) (PoC3 and PoC3a), a teleost species with important economic value. PoC3 is composed of 1655 amino acid residues, contains the six domains and highly conserved GCGEQ sequence of the C3 family. We found that PoC3 expression occurred in nine different tissues and was upregulated by bacterial challenge. In serum, PoC3 was able to bind to a broad-spectrum of bacteria, and purified native PoC3 could directly kill specific pathogen. When PoC3 expression in Japanese flounder was knocked down ...
Source: Frontiers in Immunology - February 25, 2022 Category: Allergy & Immunology Source Type: research

Genes, Vol. 13, Pages 413: HNF1A POU Domain Mutations Found in Japanese Liver Cancer Patients Cause Downregulation of HNF4A Promoter Activity with Possible Disruption in Transcription Networks
roaki Taniguchi Hepatocyte nuclear factor 1A (HNF1A) is the master regulator of liver homeostasis and organogenesis and regulates many aspects of hepatocyte functions. It acts as a tumor suppressor in the liver, evidenced by the increased proliferation in HNF1A knockout (KO) hepatocytes. Hence, we postulated that any loss-of-function variation in the gene structure or composition (mutation) could trigger dysfunction, including disrupted transcriptional networks in liver cells. From the International Cancer Genome Consortium (ICGC) database of cancer genomes, we identified several HNF1A mutations located in the function...
Source: Genes - February 24, 2022 Category: Genetics & Stem Cells Authors: Effi Haque Aamir Salam Teeli Dawid Winiarczyk Masahiko Taguchi Shun Sakuraba Hidetoshi Kono Pawe ł Leszczyński Mariusz Pierzcha ła Hiroaki Taniguchi Tags: Article Source Type: research

Blockade of Uttroside B-Induced Autophagic Pro-Survival Signals Augments Its Chemotherapeutic Efficacy Against Hepatocellular Carcinoma
Our previous study has demonstrated that Uttroside B (Utt-B), a saponin isolated from the leaves of Solanum nigrum Linn induces apoptosis in hepatic cancer cells and exhibits a remarkable growth inhibition of Hepatocellular Carcinoma (HCC). Our innovation has been granted a patent from the US (US 2019/0160088A1), Canada (3,026,426.), Japan (JP2019520425) and South Korea (KR1020190008323) and the technology have been transferred commercially to Q Biomed, a leading US-based Biotech company. Recently, the compound received approval as ‘Orphan Drug’ against HCC from US FDA, which reveals the clinical relevance of evaluatin...
Source: Frontiers in Oncology - February 8, 2022 Category: Cancer & Oncology Source Type: research

GSE178669 Next Generation Sequencing Facilitates Quantitative Analysis of Control siRNA, RAB11A siRNA1 and RAB11A siRNA2 suppressed EBC-1 cell's Transcriptomes
This study aims to find cellular pathway changes in RAB11A suppressed in lung squamous cell carcinoma cell line EBC-1 compared to control and to evaluate downregulating genes by RAB11A suppression by siRNA. Total RNA was extracted from EBC-1 transfected with control siRNA and RAB11A specific siRNAs using the RNeasy mini kit (Qiagen, Hilden, Germany). CAGE library preparation, sequencing, mapping, and gene expression and motif discovery analysis were performed by DNAFORM (Yokohama, Japan). In brief, RNA quality was assessed by Bioanalyzer (Agilent) to ensure that RIN (RNA integrity number) is over 7.0 and A260/280 and 260/2...
Source: GEO: Gene Expression Omnibus - June 23, 2021 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research

Linarin Protects the Kidney against Ischemia/Reperfusion Injury via the Inhibition of Bioactive ETS2/IL-12.
Abstract Ischemia/reperfusion injury (IRI), a participant in acute kidney injury (AKI), can occur as a series of pathological processes such as inflammation. Linarin (LIN) has been widely used for different diseases. To confirm the anti-inflammatory value and relevant mechanism of LIN during IRI, in vivo and vitro models were established. LIN or dissolvent was given, and histologic analysis, quantitative (q)RT-PCR, serum creatinine and blood urea nitrogen testing were used to evaluate kidney injury. Microarray analysis, protein-protein interaction (PPI) analysis and molecular docking were used to identify the targ...
Source: Biological and Pharmaceutical Bulletin - January 6, 2021 Category: Drugs & Pharmacology Authors: Chengyu Y, Long Z, Bin Z, Hong L, Xuefei S, Congjuan L, Caixia C, Yan X Tags: Biol Pharm Bull Source Type: research

Yi-Zhi-Fang-Dai Formula Exerts a Protective Effect on the Injury of Tight Junction Scaffold Proteins in Vitro and in Vivo by Mediating Autophagy through Regulation of the RAGE/CaMKK β/AMPK/mTOR Pathway.
This study aimed to explore the underlying mechanism of YZFDF in alleviating the injury of TJ proteins. We examined the impacts of YZFDF on autophagy-related proteins and the histopathology of Aβ in the APP/PS1 double-transgenic male mice. We then performed the free intracellular calcium levels [Ca2+]i analysis and the cognitive behavior test of the AD model. Our results showed that YZFDF ameliorated the injury of TJ proteins by reducing the mRNA transcription and expression of the receptor for advanced glycation end-products (RAGE), the levels of [Ca2+]i, calmodulin-dependent protein kinase β (CaMKKβ), phosphorylated A...
Source: Biological and Pharmaceutical Bulletin - December 4, 2020 Category: Drugs & Pharmacology Authors: Chan Y, Chen W, Chen Y, Lv Z, Wan W, Li Y, Zhang C Tags: Biol Pharm Bull Source Type: research

Involvement of CD73 and A2B receptor in radiation-induced DNA damage response and cell migration in human glioblastoma A172 cells.
Abstract Glioblastoma is the most common malignant tumor of the central nervous system and is treated with a combination of surgery, radiation and chemotherapy. However, the tumor often acquires radiation resistance, which is characterized by an increased DNA damage response (DDR). Here, we show that CD73, which generates extracellular adenosine from ATP, and A2B receptor, which is activated by adenosine, are involved in the γ-radiation-induced DDR and the enhanced migration ability of human glioblastoma cell line A172. To investigate DDR, we evaluated ATM activation and focus formation of γH2AX and 53BP1 in the...
Source: Biological and Pharmaceutical Bulletin - December 1, 2020 Category: Drugs & Pharmacology Authors: Kitabatake K, Kaji T, Tsukimoto M Tags: Biol Pharm Bull Source Type: research