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CRISPR-Cas13a-Triggered DNAzyme Signal Amplification-Based Colorimetric miRNA Detection Method and Its Application in Evaluating the Anxiety
Appl Biochem Biotechnol. 2024 Apr 23. doi: 10.1007/s12010-024-04951-1. Online ahead of print.ABSTRACTThe development of a bio-sensing strategy based on CRISPR/Cas that is exceptionally sensitive is crucial for the identification of trace molecules. Colorimetric miRNA detection utilizing CRISPR/Cas13a-triggered DNAzyme signal amplification was described in this article. The developed strategy was implemented for miRNA-21 detection as a proof of concept. The cleavage activity of Cas13a was triggered when the target molecule bonded to the Cas13a-crRNA complex and cleaved uracil ribonucleotides (rU) in the substrate probe. As ...
Source: Applied Biochemistry and Biotechnology - April 23, 2024 Category: Biochemistry Authors: Na Yan Zhiwei Hu Lingling Zhang Source Type: research

Synthesis of New Multifunctional Linolenic Acid Vanillyl Ester and Investigation of Antioxidant and Antibacterial Activities
Appl Biochem Biotechnol. 2024 Apr 22. doi: 10.1007/s12010-024-04945-z. Online ahead of print.ABSTRACTVanillyl alcohol (VA) possesses potent antioxidant activity, yet its applicability is hindered by its limited solubility in emulsions or non-polar organic solvents. Conversely, long-chain polyunsaturated fatty acids exhibit antibacterial properties. The combination of these compounds offers the prospect of developing novel phenolic lipid compounds with dual antioxidant and antibacterial activities, alongside enhanced solubility capabilities. In this investigation, linolenic acid vanillyl ester (LAVE) was synthesized from VA...
Source: Applied Biochemistry and Biotechnology - April 22, 2024 Category: Biochemistry Authors: Jennifer Indra Marvella Hyung Kwoun Kim Source Type: research

Mild γ-Butyrolactone/Water Pretreatment for Highly Efficient Sugar Production from Corn Stover
In this study, γ-butyrolactone/water (GBL/H2O) was explored as a mild, efficient, and cost-effective binary solvent pretreatment to enhance hydrolyzability of corn stover (CS). Key pretreatment parameters-reaction time, temperature, and H2SO4 concentration-were systematically investigated for their effects on the physicochemical properties of CS. Specifically, increased temperature and acid concentration significantly decreased cellulose crystallinity (from 1.39 for untreated CS to 1.04 for CS pretreated by GBL/H2O with 100 mM H2SO4 at 120 °C for 1 h) and promoted lignin removal (47.3% for CS pretreated by GBL/H2O with 1...
Source: Applied Biochemistry and Biotechnology - April 8, 2024 Category: Biochemistry Authors: Yu Yang Xueliang Ma Manzhu Wang Xinyi Ji Long Li Ziyu Liu Jiangyao Wang Yujin Ren Lili Jia Source Type: research

Mild γ-Butyrolactone/Water Pretreatment for Highly Efficient Sugar Production from Corn Stover
In this study, γ-butyrolactone/water (GBL/H2O) was explored as a mild, efficient, and cost-effective binary solvent pretreatment to enhance hydrolyzability of corn stover (CS). Key pretreatment parameters-reaction time, temperature, and H2SO4 concentration-were systematically investigated for their effects on the physicochemical properties of CS. Specifically, increased temperature and acid concentration significantly decreased cellulose crystallinity (from 1.39 for untreated CS to 1.04 for CS pretreated by GBL/H2O with 100 mM H2SO4 at 120 °C for 1 h) and promoted lignin removal (47.3% for CS pretreated by GBL/H2O with 1...
Source: Applied Biochemistry and Biotechnology - April 8, 2024 Category: Biochemistry Authors: Yu Yang Xueliang Ma Manzhu Wang Xinyi Ji Long Li Ziyu Liu Jiangyao Wang Yujin Ren Lili Jia Source Type: research

Mild γ-Butyrolactone/Water Pretreatment for Highly Efficient Sugar Production from Corn Stover
In this study, γ-butyrolactone/water (GBL/H2O) was explored as a mild, efficient, and cost-effective binary solvent pretreatment to enhance hydrolyzability of corn stover (CS). Key pretreatment parameters-reaction time, temperature, and H2SO4 concentration-were systematically investigated for their effects on the physicochemical properties of CS. Specifically, increased temperature and acid concentration significantly decreased cellulose crystallinity (from 1.39 for untreated CS to 1.04 for CS pretreated by GBL/H2O with 100 mM H2SO4 at 120 °C for 1 h) and promoted lignin removal (47.3% for CS pretreated by GBL/H2O with 1...
Source: Applied Biochemistry and Biotechnology - April 8, 2024 Category: Biochemistry Authors: Yu Yang Xueliang Ma Manzhu Wang Xinyi Ji Long Li Ziyu Liu Jiangyao Wang Yujin Ren Lili Jia Source Type: research

Mild γ-Butyrolactone/Water Pretreatment for Highly Efficient Sugar Production from Corn Stover
In this study, γ-butyrolactone/water (GBL/H2O) was explored as a mild, efficient, and cost-effective binary solvent pretreatment to enhance hydrolyzability of corn stover (CS). Key pretreatment parameters-reaction time, temperature, and H2SO4 concentration-were systematically investigated for their effects on the physicochemical properties of CS. Specifically, increased temperature and acid concentration significantly decreased cellulose crystallinity (from 1.39 for untreated CS to 1.04 for CS pretreated by GBL/H2O with 100 mM H2SO4 at 120 °C for 1 h) and promoted lignin removal (47.3% for CS pretreated by GBL/H2O with 1...
Source: Applied Biochemistry and Biotechnology - April 8, 2024 Category: Biochemistry Authors: Yu Yang Xueliang Ma Manzhu Wang Xinyi Ji Long Li Ziyu Liu Jiangyao Wang Yujin Ren Lili Jia Source Type: research

Mild γ-Butyrolactone/Water Pretreatment for Highly Efficient Sugar Production from Corn Stover
In this study, γ-butyrolactone/water (GBL/H2O) was explored as a mild, efficient, and cost-effective binary solvent pretreatment to enhance hydrolyzability of corn stover (CS). Key pretreatment parameters-reaction time, temperature, and H2SO4 concentration-were systematically investigated for their effects on the physicochemical properties of CS. Specifically, increased temperature and acid concentration significantly decreased cellulose crystallinity (from 1.39 for untreated CS to 1.04 for CS pretreated by GBL/H2O with 100 mM H2SO4 at 120 °C for 1 h) and promoted lignin removal (47.3% for CS pretreated by GBL/H2O with 1...
Source: Applied Biochemistry and Biotechnology - April 8, 2024 Category: Biochemistry Authors: Yu Yang Xueliang Ma Manzhu Wang Xinyi Ji Long Li Ziyu Liu Jiangyao Wang Yujin Ren Lili Jia Source Type: research

Mild γ-Butyrolactone/Water Pretreatment for Highly Efficient Sugar Production from Corn Stover
In this study, γ-butyrolactone/water (GBL/H2O) was explored as a mild, efficient, and cost-effective binary solvent pretreatment to enhance hydrolyzability of corn stover (CS). Key pretreatment parameters-reaction time, temperature, and H2SO4 concentration-were systematically investigated for their effects on the physicochemical properties of CS. Specifically, increased temperature and acid concentration significantly decreased cellulose crystallinity (from 1.39 for untreated CS to 1.04 for CS pretreated by GBL/H2O with 100 mM H2SO4 at 120 °C for 1 h) and promoted lignin removal (47.3% for CS pretreated by GBL/H2O with 1...
Source: Applied Biochemistry and Biotechnology - April 8, 2024 Category: Biochemistry Authors: Yu Yang Xueliang Ma Manzhu Wang Xinyi Ji Long Li Ziyu Liu Jiangyao Wang Yujin Ren Lili Jia Source Type: research

Mild γ-Butyrolactone/Water Pretreatment for Highly Efficient Sugar Production from Corn Stover
In this study, γ-butyrolactone/water (GBL/H2O) was explored as a mild, efficient, and cost-effective binary solvent pretreatment to enhance hydrolyzability of corn stover (CS). Key pretreatment parameters-reaction time, temperature, and H2SO4 concentration-were systematically investigated for their effects on the physicochemical properties of CS. Specifically, increased temperature and acid concentration significantly decreased cellulose crystallinity (from 1.39 for untreated CS to 1.04 for CS pretreated by GBL/H2O with 100 mM H2SO4 at 120 °C for 1 h) and promoted lignin removal (47.3% for CS pretreated by GBL/H2O with 1...
Source: Applied Biochemistry and Biotechnology - April 8, 2024 Category: Biochemistry Authors: Yu Yang Xueliang Ma Manzhu Wang Xinyi Ji Long Li Ziyu Liu Jiangyao Wang Yujin Ren Lili Jia Source Type: research

MiR-449b-5p Ameliorates Hypoxia-induced Cardiomyocyte Injury through Activating PI3K/AKT Pathway by Targeting BCL2L13
In this study, AC16 human cardiomyocytes underwent hypoxic conditions for durations of 24, 48, and 72 h. We observed that miR-449b-5p expression was significantly downregulated in hypoxic AC16 cardiomyocytes. Elevating the levels of miR-449b-5p in these cells resulted in enhanced cell survival, diminished release of LDH, and a reduction in cell apoptosis and oxidative stress using CCK-8, LDH assays, flow cytometry, TUNEL staining, and various commercial kits. Conversely, reducing miR-449b-5p levels resulted in the opposite effects. Through bioinformatics analysis and luciferase reporter assays, BCL2-like 13 (BCL2L13) was d...
Source: Applied Biochemistry and Biotechnology - April 6, 2024 Category: Biochemistry Authors: Yang Jiang Zeyan Liu Li Ye Jinglin Cheng Jun Wan Source Type: research

MiR-449b-5p Ameliorates Hypoxia-induced Cardiomyocyte Injury through Activating PI3K/AKT Pathway by Targeting BCL2L13
In this study, AC16 human cardiomyocytes underwent hypoxic conditions for durations of 24, 48, and 72 h. We observed that miR-449b-5p expression was significantly downregulated in hypoxic AC16 cardiomyocytes. Elevating the levels of miR-449b-5p in these cells resulted in enhanced cell survival, diminished release of LDH, and a reduction in cell apoptosis and oxidative stress using CCK-8, LDH assays, flow cytometry, TUNEL staining, and various commercial kits. Conversely, reducing miR-449b-5p levels resulted in the opposite effects. Through bioinformatics analysis and luciferase reporter assays, BCL2-like 13 (BCL2L13) was d...
Source: Applied Biochemistry and Biotechnology - April 6, 2024 Category: Biochemistry Authors: Yang Jiang Zeyan Liu Li Ye Jinglin Cheng Jun Wan Source Type: research

MiR-449b-5p Ameliorates Hypoxia-induced Cardiomyocyte Injury through Activating PI3K/AKT Pathway by Targeting BCL2L13
In this study, AC16 human cardiomyocytes underwent hypoxic conditions for durations of 24, 48, and 72 h. We observed that miR-449b-5p expression was significantly downregulated in hypoxic AC16 cardiomyocytes. Elevating the levels of miR-449b-5p in these cells resulted in enhanced cell survival, diminished release of LDH, and a reduction in cell apoptosis and oxidative stress using CCK-8, LDH assays, flow cytometry, TUNEL staining, and various commercial kits. Conversely, reducing miR-449b-5p levels resulted in the opposite effects. Through bioinformatics analysis and luciferase reporter assays, BCL2-like 13 (BCL2L13) was d...
Source: Applied Biochemistry and Biotechnology - April 6, 2024 Category: Biochemistry Authors: Yang Jiang Zeyan Liu Li Ye Jinglin Cheng Jun Wan Source Type: research

Utilizing a Fe < sub > 3 < /sub > O < sub > 4 < /sub > Magnetite Nanoparticle for Anode Modification in a Microbial Desalination Cell to Treat Saltwater
In this study, a three-chamber microbial desalination cell was developed to treat seawater using a synthesize Fe3O4 magnetite nanoparticle (MNP)-modified anode. The impact of different performance parameters, such as temperature, pH, and concentrations of NPs, has been investigated in order to assess the performance of three-chamber MDCs in terms of energy recovery and salt removal. The evaluation criteria of the system included multiple factors such as chemical oxygen demand (COD), Coulombic efficiency (CE), desalination efficiency, as well as system aspects including voltage generation and power density. The highest COD%...
Source: Applied Biochemistry and Biotechnology - April 4, 2024 Category: Biochemistry Authors: Shruti Singh Ankit Kumar Soumya Pandit Arpita Roy Dibyajit Lahiri Saad Alghamdi Mazen Almehmadi Ahad Amer Alsaiari Mamdouh Allahyani Source Type: research

Utilizing a Fe < sub > 3 < /sub > O < sub > 4 < /sub > Magnetite Nanoparticle for Anode Modification in a Microbial Desalination Cell to Treat Saltwater
In this study, a three-chamber microbial desalination cell was developed to treat seawater using a synthesize Fe3O4 magnetite nanoparticle (MNP)-modified anode. The impact of different performance parameters, such as temperature, pH, and concentrations of NPs, has been investigated in order to assess the performance of three-chamber MDCs in terms of energy recovery and salt removal. The evaluation criteria of the system included multiple factors such as chemical oxygen demand (COD), Coulombic efficiency (CE), desalination efficiency, as well as system aspects including voltage generation and power density. The highest COD%...
Source: Applied Biochemistry and Biotechnology - April 4, 2024 Category: Biochemistry Authors: Shruti Singh Ankit Kumar Soumya Pandit Arpita Roy Dibyajit Lahiri Saad Alghamdi Mazen Almehmadi Ahad Amer Alsaiari Mamdouh Allahyani Source Type: research

Utilizing a Fe < sub > 3 < /sub > O < sub > 4 < /sub > Magnetite Nanoparticle for Anode Modification in a Microbial Desalination Cell to Treat Saltwater
In this study, a three-chamber microbial desalination cell was developed to treat seawater using a synthesize Fe3O4 magnetite nanoparticle (MNP)-modified anode. The impact of different performance parameters, such as temperature, pH, and concentrations of NPs, has been investigated in order to assess the performance of three-chamber MDCs in terms of energy recovery and salt removal. The evaluation criteria of the system included multiple factors such as chemical oxygen demand (COD), Coulombic efficiency (CE), desalination efficiency, as well as system aspects including voltage generation and power density. The highest COD%...
Source: Applied Biochemistry and Biotechnology - April 4, 2024 Category: Biochemistry Authors: Shruti Singh Ankit Kumar Soumya Pandit Arpita Roy Dibyajit Lahiri Saad Alghamdi Mazen Almehmadi Ahad Amer Alsaiari Mamdouh Allahyani Source Type: research