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Adenovirus-mediated delivery of siRNA targeting TM4SF4 attenuated liver cancer cell growth in vitro and in vivo.
In this study, we developed an adenoviral vector harboring an effective siRNA targeting TM4SF4 (AdSiTM4SF4) and identified its function in suppression of tumor cell growth. It was confirmed that TM4SF4 was overexpressed in HCC tissues compared with its paired non-tumor tissues by western blot analysis and immunohistochemistry. Remarkably, it was more abundant on the cell surface of HCC cells. The signals of ectopically expressed TM4SF4 in four cell lines dramatically localized in the plasma membrane, slightly in the cytoplasm, and absent in the nucleus, demonstrating that TM4SF4 is a membrane protein. Targeting TM4SF4 by A...
Source: Acta Biochimica et Biophysica Sinica - January 7, 2013 Category: Biochemistry Authors: Wang L, Feng J, Da L, Li Y, Li Z, Zhao M Tags: Acta Biochim Biophys Sin (Shanghai) Source Type: research

TonEBP Suppresses the HO-1 Gene by Blocking Recruitment of Nrf2 to Its Promoter
Discussion Dynamic changes in the functional phenotype of macrophages are associated with pathogenesis of inflammatory diseases (5–7). TonEBP primes macrophages toward an M1 phenotype, which has pro-inflammatory properties. TonEBP does this by promoting expression of pro-inflammatory genes via interaction with NF-κB (36) and by binding directly to the promoter (37, 64). In addition, TonEBP suppresses expression of the anti-inflammatory cytokine IL-10 by limiting chromatin access to the promoter (37). The pro-inflammatory function of TonEBP suggests that inhibiting its expression or activation could suppres...
Source: Frontiers in Immunology - April 17, 2019 Category: Allergy & Immunology Source Type: research

Recombinant adenovirus encoding FAT10 small interfering RNA inhibits HCC growth in vitro and in vivo.
Abstract Hepatocellular carcinoma is an aggressive and rapidly fatal malignancy representing the common cancer worldwide. The specific cellular gene involved in carcinogenesis has nor been full charactered. The ubiquitin-like modifier FAT10, a recently reported to be overexpressed in 90% of hepatocellular carcinoma carcinomas, was attributed to transcriptional upregulation upon the loss of p53 and induced chromosome instability in long-term in vitro culture. However, the exact function of FAT10 in Hepatocellular carcinoma is not clear. In the present study, we utilized adenovirus-mediated RNA interference to knock...
Source: Experimental and Molecular Pathology - January 14, 2014 Category: Pathology Authors: Chen J, Zi W, Yang Z Tags: Exp Mol Pathol Source Type: research

Argonaute 2 promotes angiogenesis via the PTEN/VEGF signaling pathway in human hepatocellular carcinoma.
CONCLUSION: A direct relationship exists between the miRNA processing machinery AGO2 and HCC angiogenesis that is mediated by the AGO2/PTEN/VEGF signaling pathway. The results suggest the high value of Ago2 knockdown in anti-angiogenesis therapy for HCC. PMID: 25937637 [PubMed - as supplied by publisher]
Source: Acta Pharmacologica Sinica - May 4, 2015 Category: Drugs & Pharmacology Authors: Ye ZL, Huang Y, Li LF, Zhu HL, Gao HX, Liu H, Lv SQ, Xu ZH, Zheng LN, Liu T, Zhang JL, Jin HJ, Qian QJ Tags: Acta Pharmacol Sin Source Type: research

Silencing NOB1 enhances doxorubicin antitumor activity of the papillary thyroid carcinoma in vitro and in vivo.
Authors: Liu J, Dong BF, Wang PS, Ren PY, Xue S, Zhang XΝ, Han Z, Chen G Abstract Doxorubicin (DOX), a broad‑spectrum anthra-cyclin, is in wide clinical use for the treatment and prevention of thyroid cancer. However, the effectiveness of the treatment remains limited due to inherent tumor resistance to DOX. Results of a previous study demonstrated that downregulation of NIN1/RPN12 binding protein 1 homolog (NOB1) expression via adenovirus expression vector carrying NOB1 siRNA (Ad/sh-NOB1) induced cancer apoptosis and increased the radiosensitivity of papillary thyroid carcinoma (PTC) cells. However, whether kn...
Source: Oncology Reports - January 21, 2015 Category: Cancer & Oncology Tags: Oncol Rep Source Type: research

Haploinsufficiency of the c-myc transcriptional repressor FIR, as a dominant negative-alternative splicing model, promoted p53-dependent T-cell acute lymphoblastic leukemia progression by activating Notch1.
In conclusion, the alternative splicing of FIR, which generates FIRΔexon2, may contribute to both colorectal carcinogenesis and leukemogenesis. PMID: 25671302 [PubMed - as supplied by publisher]
Source: Oncotarget - February 13, 2015 Category: Cancer & Oncology Tags: Oncotarget Source Type: research

Calpain-10 Activity Underlies Angiotensin II-induced Aldosterone Production in an Adrenal Glomerulosa Cell Model.
Abstract Aldosterone is a steroid hormone important in the regulation of blood pressure. Aberrant production of aldosterone results in the development and progression of diseases including hypertension and congestive heart failure; therefore, a complete understanding of aldosterone production is important for developing more effective treatments. Angiotensin II (AngII) regulates steroidogenesis, in part through its ability to increase intracellular calcium levels. Calcium can activate calpains, proteases classified as typical or atypical based on the presence or absence of penta-EF-hands, that are involved in vari...
Source: Endocrinology - April 2, 2015 Category: Endocrinology Authors: Seremwe M, Schnellmann RG, Bollag WB Tags: Endocrinology Source Type: research

Anti-tumor effect of LATS2 on liver cancer death: Role of DRP1-mediated mitochondrial division and the Wnt/β-catenin pathway
Publication date: June 2019Source: Biomedicine & Pharmacotherapy, Volume 114Author(s): Lijuan Zhang, Shuping Li, Rong Wang, Changyuan Chen, Wen Ma, Hongyi CaiAbstractLarge tumor suppressor 2 (LATS2), an important mediator of the cell apoptotic response pathway, has been linked to the progression of several cancers. Here, we described the molecular feature of LATS2 as a novel antitumor factor in liver cancer cells in vitro. Western blotting was used to detect the expression of LATS2 and its downstream factors. ELISA, immunofluorescence, and flow cytometry were used to evaluate the alterations of mitochondrial function in re...
Source: Biomedicine and Pharmacotherapy - April 12, 2019 Category: Drugs & Pharmacology Source Type: research

Anti-tumor effect of LATS2 on liver cancer death: Role of DRP1-mediated mitochondrial division and the Wnt/ β-catenin pathway.
Anti-tumor effect of LATS2 on liver cancer death: Role of DRP1-mediated mitochondrial division and the Wnt/β-catenin pathway. Biomed Pharmacother. 2019 Apr 10;114:108825 Authors: Zhang L, Li S, Wang R, Chen C, Ma W, Cai H Abstract Large tumor suppressor 2 (LATS2), an important mediator of the cell apoptotic response pathway, has been linked to the progression of several cancers. Here, we described the molecular feature of LATS2 as a novel antitumor factor in liver cancer cells in vitro. Western blotting was used to detect the expression of LATS2 and its downstream factors. ELISA, immunofluorescence, ...
Source: Biomedicine and pharmacotherapy = Biomedecine and pharmacotherapie - April 9, 2019 Category: Drugs & Pharmacology Authors: Zhang L, Li S, Wang R, Chen C, Ma W, Cai H Tags: Biomed Pharmacother Source Type: research

Gene Therapy Leaves a Vicious Cycle
Reena Goswami1, Gayatri Subramanian2, Liliya Silayeva1, Isabelle Newkirk1, Deborah Doctor1, Karan Chawla2, Saurabh Chattopadhyay2, Dhyan Chandra3, Nageswararao Chilukuri1 and Venkaiah Betapudi1,4* 1Neuroscience Branch, Research Division, United States Army Medical Research Institute of Chemical Defense, Aberdeen, MD, United States 2Department of Medical Microbiology and Immunology, University of Toledo College of Medicine and Life Sciences, Toledo, OH, United States 3Roswell Park Comprehensive Cancer Center, Buffalo, NY, United States 4Department of Physiology and Biophysics, Case Western Reserve University, Clev...
Source: Frontiers in Oncology - April 23, 2019 Category: Cancer & Oncology Source Type: research

Hippo/Mst1 overexpression induces mitochondrial death in head and neck squamous cell carcinoma via activating β-catenin/Drp1 pathway.
Abstract Mammalian Ste20-like kinase 1 (Mst1) is associated with cell apoptosis. In the current study, we explored the regulatory effects of Mst1 on squamous cell carcinoma of the head and neck (SCCHN) in vitro. SCCHN Cal27 cells and Tu686 cells were transfected with adenovirus-loaded Mst1 to detect the role of Mst1 in cell viability. Then, siRNA against Drp1 was transfected into cells to evaluate the influence of mitochondrial fission in cancer survival. Our data illustrated that Mst1 overexpression promoted SCCHN Cal27 cell and Tu686 cell death via activating mitochondria-related apoptosis. Cells transfected wit...
Source: Cell Stress and Chaperones - May 23, 2019 Category: Cytology Authors: Ma C, Fan L, Wang J, Hao L, He J Tags: Cell Stress Chaperones Source Type: research

ETS-1/c-Met drives resistance to sorafenib in hepatocellular carcinoma
CONCLUSION: c-Met overexpression and activation is an essential mechanism of sorafenib resistance in HCC. Combination therapy of sorafenib plus c-Met inhibitor overcame the resistance of sorafenib-targeted therapy for HCC.PMID:36915773 | PMC:PMC10006745
Source: Cell Research - March 14, 2023 Category: Cytology Authors: Yongfang Ma Xueke Liu Xiaolong Tang Source Type: research

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