Biochemical analysis leads to improved orthogonal bioluminescent tools
Chembiochem. 2023 Jan 2. doi: 10.1002/cbic.202200726. Online ahead of print.ABSTRACTEngineered luciferase-luciferin pairs have expanded the number of cellular targets that can be visualized in tandem. While light production relies on selective processing of synthetic luciferins by mutant luciferases, little is known about the origin of selectivity. The development of new and improved pairs requires a better understanding of the structure-function relationship of bioluminescent probes. In this work, we report a biochemical approach to assessing and optimizing two popular bioluminescent pairs: Cashew/D-luc and Pecan/4'-BrLuc...
Source: Chembiochem - January 2, 2023 Category: Biochemistry Authors: Sierra J Williams Jordan A Gewing-Mullins Whitney K Lieberman Bethany Kolbaba-Kartchner Reema Iqbal Hana M Burgess Clair M Colee Marya Y Ornelas Edison S Reid-McLaughlin Jeremy H Mills Jennifer A Prescher Aaron Morgan Leconte Source Type: research
Biochemical analysis leads to improved orthogonal bioluminescent tools
Chembiochem. 2023 Jan 2. doi: 10.1002/cbic.202200726. Online ahead of print.ABSTRACTEngineered luciferase-luciferin pairs have expanded the number of cellular targets that can be visualized in tandem. While light production relies on selective processing of synthetic luciferins by mutant luciferases, little is known about the origin of selectivity. The development of new and improved pairs requires a better understanding of the structure-function relationship of bioluminescent probes. In this work, we report a biochemical approach to assessing and optimizing two popular bioluminescent pairs: Cashew/D-luc and Pecan/4'-BrLuc...
Source: Chembiochem - January 2, 2023 Category: Biochemistry Authors: Sierra J Williams Jordan A Gewing-Mullins Whitney K Lieberman Bethany Kolbaba-Kartchner Reema Iqbal Hana M Burgess Clair M Colee Marya Y Ornelas Edison S Reid-McLaughlin Jeremy H Mills Jennifer A Prescher Aaron Morgan Leconte Source Type: research
Viruses, Vol. 15, Pages 39: Identification of Seven Additional Genome Segments of Grapevine-Associated Jivivirus 1
ie Lefebvre
Jiviruses are a group of recently described viruses characterized with a tripartite genome and having affinities with Virgaviridae (RNA1 and 2) and Flaviviridae (RNA3). Using a combination of high-throughput sequencing, datamining and RT-PCR approaches, we demonstrate here that in grapevine samples infected by grapevine-associated jivivirus 1 (GaJV-1) up to 7 additional molecules can be consistently detected with conserved 5′ and 3′ non-coding regions in common with the three previously identified GaJV-1 genomic RNAs. RNA4, RNA5, RNA6, RNA7, RNA8 and RNA10, together with a recomb...
Source: Viruses - December 22, 2022 Category: Virology Authors: Thierry Candresse Laurence Svanella-Dumas Armelle Marais Flora Depasse Chantal Faure Marie Lefebvre Tags: Article Source Type: research
[ASAP] Metabolomics and Transcriptomics Analyses Reveal Regulatory Networks Associated with Fatty Acid Accumulation in Pecan Kernels
Journal of Agricultural and Food ChemistryDOI: 10.1021/acs.jafc.2c06947 (Source: Journal of Agricultural and Food Chemistry)
Source: Journal of Agricultural and Food Chemistry - December 6, 2022 Category: Food Science Authors: Chengcai Zhang, Huadong Ren, Xiaohua Yao, Kailiang Wang, Jun Chang, and Weizhong Shao Source Type: research
Catalytic DNA-Assisted Mass Production of Arbitrary Single-Stranded DNA
Angew Chem Int Ed Engl. 2022 Nov 8. doi: 10.1002/anie.202212011. Online ahead of print.ABSTRACTSynthetic single-stranded (ss) DNA is a cornerstone for life and materials science, yet the purity, quantity, length, and customizability of synthetic DNA are still limiting in various applications. Here we present PECAN, paired-end cutting assisted by DNAzymes (DNA enzymes or deoxyribozymes), which enables mass production of ssDNA of arbitrary sequence (up to 7,000 nucleotides, or nt) with single-base precision. At the core of PECAN technique are two newly-identified classes of DNAzymes, each robustly self-hydrolyzing with minim...
Source: Angewandte Chemie - November 8, 2022 Category: Chemistry Authors: Qiao Zhang Kai Xia Meng Jiang Qingting Li Weigang Chen Mingzhe Han Wei Li Rongqin Ke Fei Wang Yongxing Zhao Yuehua Liu Chunhai Fan Hongzhou Gu Source Type: research
Molecules, Vol. 27, Pages 6876: Brazilian Agroindustrial Wastes as a Potential Resource of Bioative Compounds and their Antimicrobial and Antioxidant Activities
Silveira da Rosa
The study of the recovery of bioactive compounds from natural resources and its implications in several areas is very significant for the scientific community. This work aimed to study Brazilian agroindustrial wastes’ antioxidant and antimicrobial activities using green extraction. Olive leaves, jaboticaba peel, araçá peel, and pecan nut shells were evaluated under four conditions: (1) convective-drying and aqueous extraction, (2) convective-drying and ethanolic extraction, (3) freeze-drying and aqueous extraction, and (4) freeze-drying and ethanolic extracti...
Source: Molecules - October 13, 2022 Category: Chemistry Authors: Alaor Val ério Filho Luisa Bataglin Avila Douglas Hardt Lacorte Thamiris Renata Martiny Vanessa Rosseto Caroline Costa Moraes Guilherme Luiz Dotto Neftali Lenin Villarreal Carreno Gabriela Silveira da Rosa Tags: Article Source Type: research
Effects of Plant Growth-Promoting Rhizobacteria on the Growth and Soil Microbial Community of Carya illinoinensis
This study provides basic reference data regarding the use of PGPR to improve the microecological environment and promote the growth and development of C. illinoinensis plants.PMID:36209341 | DOI:10.1007/s00284-022-03027-9 (Source: Current Microbiology)
Source: Current Microbiology - October 8, 2022 Category: Microbiology Authors: Ji-Wu Shi Lan-Xiang Lu Hui-Min Shi Jian-Ren Ye Source Type: research
Effects of Plant Growth-Promoting Rhizobacteria on the Growth and Soil Microbial Community of Carya illinoinensis
This study provides basic reference data regarding the use of PGPR to improve the microecological environment and promote the growth and development of C. illinoinensis plants.PMID:36209341 | DOI:10.1007/s00284-022-03027-9 (Source: Current Microbiology)
Source: Current Microbiology - October 8, 2022 Category: Microbiology Authors: Ji-Wu Shi Lan-Xiang Lu Hui-Min Shi Jian-Ren Ye Source Type: research
Effects of Plant Growth-Promoting Rhizobacteria on the Growth and Soil Microbial Community of Carya illinoinensis
This study provides basic reference data regarding the use of PGPR to improve the microecological environment and promote the growth and development of C. illinoinensis plants.PMID:36209341 | DOI:10.1007/s00284-022-03027-9 (Source: Current Microbiology)
Source: Current Microbiology - October 8, 2022 Category: Microbiology Authors: Ji-Wu Shi Lan-Xiang Lu Hui-Min Shi Jian-Ren Ye Source Type: research
One-class model with two decision thresholds for the rapid detection of cashew nuts adulteration by other nuts
Talanta. 2022 Sep 14;253:123916. doi: 10.1016/j.talanta.2022.123916. Online ahead of print.ABSTRACTA green screening method to determine cashew nut adulteration with Brazilian nut, pecan nut, macadamia nut and peanut was proposed. The method was based on the development of a one-class soft independent modelling of class analogy (SIMCA) model for non-adulterated cashew nuts using near-infrared (NIR) spectra obtained with portable equipment. Once the model is established, the assignment of unknown samples depends on the threshold established for the authentic class, which is a key aspect in any screening approach. The author...
Source: Talanta - September 20, 2022 Category: Chemistry Authors: Gl òria Rovira Carolina Sheng Whei Miaw M ário Lúcio Campos Martins Marcelo Martins Sena Scheilla Vitorino Carvalho de Souza M Pilar Callao Itziar Ruis ánchez Source Type: research
One-class model with two decision thresholds for the rapid detection of cashew nuts adulteration by other nuts
Talanta. 2022 Sep 14;253:123916. doi: 10.1016/j.talanta.2022.123916. Online ahead of print.ABSTRACTA green screening method to determine cashew nut adulteration with Brazilian nut, pecan nut, macadamia nut and peanut was proposed. The method was based on the development of a one-class soft independent modelling of class analogy (SIMCA) model for non-adulterated cashew nuts using near-infrared (NIR) spectra obtained with portable equipment. Once the model is established, the assignment of unknown samples depends on the threshold established for the authentic class, which is a key aspect in any screening approach. The author...
Source: Talanta - September 20, 2022 Category: Chemistry Authors: Gl òria Rovira Carolina Sheng Whei Miaw M ário Lúcio Campos Martins Marcelo Martins Sena Scheilla Vitorino Carvalho de Souza M Pilar Callao Itziar Ruis ánchez Source Type: research
A comparative study of antimicrobial, anti-quorum sensing, anti-biofilm, anti-swarming, and antioxidant activities in flower extracts of pecan (Carya illinoinensis) and chestnut (Castanea sativa)
Arch Microbiol. 2022 Sep 2;204(9):589. doi: 10.1007/s00203-022-03172-6.ABSTRACTAntibiotic resistance, which has increased rapidly in recent years because of uncontrolled and unconscious antibiotic consumption, poses a major threat to public health. The inadequacy of existing antibiotics has increased the need for new, effective, and less toxic antibiotic raw materials or antibiotic derivatives. Pecan (Carya illinoinensis) and Chestnut (Castanea sativa) flowers possess abundant pollen contents and exhibit similar morphological features. The purpose of this study was to compare these two flower extracts in terms of their ant...
Source: Archives of Microbiology - September 2, 2022 Category: Microbiology Authors: Ülkü Zeynep Üreyen Esertaş Yakup Kara Ali Osman Kili ç Sevgi Kolayli Source Type: research
A comparative study of antimicrobial, anti-quorum sensing, anti-biofilm, anti-swarming, and antioxidant activities in flower extracts of pecan (Carya illinoinensis) and chestnut (Castanea sativa)
Arch Microbiol. 2022 Sep 2;204(9):589. doi: 10.1007/s00203-022-03172-6.ABSTRACTAntibiotic resistance, which has increased rapidly in recent years because of uncontrolled and unconscious antibiotic consumption, poses a major threat to public health. The inadequacy of existing antibiotics has increased the need for new, effective, and less toxic antibiotic raw materials or antibiotic derivatives. Pecan (Carya illinoinensis) and Chestnut (Castanea sativa) flowers possess abundant pollen contents and exhibit similar morphological features. The purpose of this study was to compare these two flower extracts in terms of their ant...
Source: Archives of Microbiology - September 2, 2022 Category: Microbiology Authors: Ülkü Zeynep Üreyen Esertaş Yakup Kara Ali Osman Kili ç Sevgi Kolayli Source Type: research
Angiopoietin-Like Protein Responses to Pecan-Enriched Diets Versus a Nut-Excluded Diet
Journal of Medicinal Food, Ahead of Print. (Source: Journal of Medicinal Food)
Source: Journal of Medicinal Food - August 24, 2022 Category: Nutrition Authors: Liana L. Guarneiri Chad M. Paton Jamie A. Cooper Source Type: research
Enzyme inhibitory activities of phenolic compounds in pecan and the effect on starch digestion
The objective of this study was to investigate the enzyme inhibitory activities of pecan phenolic compounds (PPC) and the effect in starch hydrolysis by in vitro simulation digestion. PPC was extracted with a solvent mixture from pecan powder and freeze-dried. PPC was tested for the inhibitory effects on α-amylase and α-glucosidase via enzyme kinetics study. Static in vitro digestion trials were conducted to evaluate the effect of intake of PPC and pecan powder on starch digestion. The PPC displayed a potent inhibition effect against α-amylase and α-glucosidase with IC50 of 77.9 μg/mL and 9.02 μg/mL, respectively. Bo...
Source: International Journal of Biological Macromolecules - August 13, 2022 Category: Biochemistry Authors: Jiannan Feng Fanbin Kong Source Type: research
