MRSA Research This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Human-Derived collagen hydrogel as an antibiotic vehicle for topical treatment of bacterial biofilms
This study aims to assess the capacity of human -derived collagen hydrogel to provide sustained antibiotic release to disrupt bacterial biofilms and decrease bacterial load while maintaining host cell viability and scaffold integrity. Human collagen harvested from flexor tendons underwent processing to yield a gellable liquid, and subsequently wa s combined with varying concentrations of gentamicin (50–500 mg/L) or clindamycin (10–100 mg/L). The elution kinetics of antibiotics from the hydrogel were analyzed using liquid chromatography-mass spectrometry. The gel was used to topically treat Methicillin-resistantStaphylo...
Source: PLoS One - May 3, 2024 Category: Biomedical Science Authors: Evan Jarman Source Type: research

 Microbacterium salsuginis sp. nov., a halotolerant actinobacterium with antimicrobial activity
Int J Syst Evol Microbiol. 2024 May;74(5). doi: 10.1099/ijsem.0.006362.ABSTRACTA novel Gram-staining-positive actinobacterium with antimicrobial activity, designated CFH 90308T, was isolated from the sediment of a salt lake in Yuncheng, Shanxi, south-western China. The isolate exhibited the highest 16S rRNA gene sequence similarities to Microbacterium yannicii G72T, Microbacterium hominis NBRC 15708T and Microbacterium xylanilyticum S3-ET (98.5, 98.4 and 98.2 %, respectively), and formed a separate clade with M. xylanilyticum S3-ET in phylogenetic trees. The strain grew at 15-40 ºC, pH 6.0-8.0 and could tolerate NaCl up t...
Source: International Journal of Systematic and Evolutionary Microbiology - May 2, 2024 Category: Microbiology Authors: Qingyang Su Yuwei Liu Tingting Xia Shuanglong Wei Caixia Cui Zhao Zhang Xudong Liu Guoxing Nie Hong Ming Source Type: research

Collaborative Cross mice have diverse phenotypic responses to infection with Methicillin-resistant Staphylococcus aureus USA300
by Aravindh Nagarajan, Kristin Scoggin, Jyotsana Gupta, Manuchehr Aminian, L. Garry Adams, Michael Kirby, David Threadgill, Helene Andrews-PolymenisStaphylococcus aureus (S.aureus) is an opportunistic pathogen causing diseases ranging from mild skin infections to life threatening conditions, including endocarditis, pneumonia, and sepsis. To identify host genes modulating this host-pathogen interaction, we infected 25 Collaborative Cross (CC) mouse strains with methicillin-resistantS.aureus (MRSA) and monitored disease progression for seven days using a surgically implanted telemetry system. CC strains varied widely in thei...
Source: PLoS Genetics - May 2, 2024 Category: Genetics & Stem Cells Authors: Aravindh Nagarajan Source Type: research

The Spx stress regulator confers high-level β-lactam resistance and decreases susceptibility to last-line antibiotics in methicillin-resistant Staphylococcus aureus 
Antimicrob Agents Chemother. 2024 May 1:e0033524. doi: 10.1128/aac.00335-24. Online ahead of print.ABSTRACTInfections caused by methicillin-resistant Staphylococcus aureus (MRSA) are a leading cause of mortality worldwide. MRSA has acquired resistance to next-generation β-lactam antibiotics through the horizontal acquisition of the mecA resistance gene. Development of high resistance is, however, often associated with additional mutations in a set of chromosomal core genes, known as potentiators, which, through poorly described mechanisms, enhance resistance. The yjbH gene was recently identified as a hot spot for adaptiv...
Source: Antimicrobial Agents and Chemotherapy - May 1, 2024 Category: Microbiology Authors: Tobias Krogh Nielsen Ida Birkj ær Petersen Lijuan Xu Maria Disen Barbuti Viktor Mebus Anni Justh Abdulelah Ahmed Alqarzaee Nicolas Jacques C écile Oury Vinai Thomas Morten Kjos Camilla Henriksen Dorte Frees Source Type: research

Photosensitive AIEgens sensitize bacteria to oxidative damage and modulate the inflammatory responses of macrophages to salvage the photodynamic therapy against MRSA
Biomaterials. 2024 Apr 26;309:122583. doi: 10.1016/j.biomaterials.2024.122583. Online ahead of print.ABSTRACTThe urgent need for antimicrobial agents to combat infections caused by multidrug-resistant bacteria facilitates the exploration of alternative strategies such as photosensitizer (PS)-mediated photoinactivation. However, increasing studies have discovered uncorrelated bactericidal activities among PSs possessing similar photodynamic and pathogen-targeted properties. To optimize the photodynamic therapy (PDT) against infections, we investigated three type-I PSs of D-π-A AIEgens TI, TBI, and TTI. The capacities of re...
Source: Biomaterials - May 1, 2024 Category: Materials Science Authors: Yifan Wu Jiangao Li Liwei Zhu Deliang Wang Jiayi Song Xiyong Yu Ying Li Ben Zhong Tang Source Type: research

Novel natural osthole-inspired amphiphiles as membrane targeting antibacterials against methicillin-resistant Staphylococcus aureus (MRSA)
Eur J Med Chem. 2024 Apr 26;271:116449. doi: 10.1016/j.ejmech.2024.116449. Online ahead of print.ABSTRACTMethicillin-resistant Staphylococcus aureus (MRSA) is a widespread pathogen causing clinical infections and is multi-resistant to many antibiotics, making it urgent need to develop novel antibacterials to combat MRSA. Herein, we designed and prepared a series of novel osthole amphiphiles 6a-6ad by mimicking the structures and function of antimicrobial peptides (AMPs). Antibacterial assays showed that osthole amphiphile 6aa strongly inhibited S. aureus and 10 clinical MRSA isolates with MIC values of 1-2 μg/mL, comparab...
Source: European Journal of Medicinal Chemistry - May 1, 2024 Category: Chemistry Authors: Ruige Yang Zihan Xue Xinhui Li Ting Xu Yan Zhong Songlin Hu Shangshang Qin Yong Guo Source Type: research

On-site detection of methicillin-resistant Staphylococcus aureus (MRSA) utilizing G-quadruplex based isothermal exponential amplification reaction (GQ-EXPAR)
Talanta. 2024 Apr 13;275:126073. doi: 10.1016/j.talanta.2024.126073. Online ahead of print.ABSTRACTMethicillin-resistant Staphylococcus aureus (MRSA) has a high incidence in infectious hospitals and communities, highlighting the need for early on-site detection due to its resistance to methicillin antibiotics. The present study introduces a highly sensitive detection system for mecA, a crucial methicillin marker, utilizing an RCA-based isothermal exponential amplification reaction. The G-quadruplex-based isothermal exponential amplification reaction (GQ-EXPAR) method designs probes to establish G-quadruplex secondary struc...
Source: Talanta - April 30, 2024 Category: Chemistry Authors: Seung Beom Seo Jina Lee Eunjung Kim Jaewoo Lim Soojin Jang Seong Uk Son Yeonwoo Jeong Taejeoon Kang Juyeon Jung Kyoung G Lee Sung-Woon Lee Kyujung Kim Eun-Kyung Lim Source Type: research

Synthesis, Crystal structure, Lipophilicity, Antioxidant, Binding interaction and Antibacterial investigation against Methicillin-Resistant Staphylococcus aureus of Ni(II) Schiff base complex: Combined Theoretical and Experimental Approaches
New J. Chem., 2024, Accepted Manuscript DOI: 10.1039/D3NJ05150A, PaperBidhan Chandra Samanta, Arpita Das, Ribhu Maity, Tuhin Sarkar, Priyanka Das, Paula Brand ão, Tithi Maity, Keka Sarkar Herein, we report the synthesis, crystal structure and antibacterial activity of a new Ni(II) complex derived from N, O donor (E)-2,4-dibromo-6-(2-(1-phenylethylimino) ethyl) phenol ligand. Both the ligand and the complex... The content of this RSS Feed (c) The Royal Society of Chemistry (Source: RSC - New J. Chem. latest articles)
Source: RSC - New J. Chem. latest articles - April 29, 2024 Category: Chemistry Authors: Bidhan Chandra Samanta Source Type: research

Underdiagnosis of Borderline oxacillin-resistant Staphylococcus aureus (BORSA) - Case series
We report five cases of BORSA blood-stream infections in a university teaching hospital. All isolates were found to be susceptible to cefoxitin using disk diffusion, resistant to oxacillin using automated MIC method, and did not harbour mecA gene. All patients were suscessfully treated with anti-MRSA antibiotics, and removal of primary sources were done if identified. A more cost-effective method for screening and diagnosis of BORSA is needed in addition to cefoxitin disk diffusion test, in order to monitor the spread, and to enable routine detection and treatment of this pathogen.PMID:38682849 (Source: Malaysian Journal of Pathology)
Source: Malaysian Journal of Pathology - April 29, 2024 Category: Pathology Authors: N Samsudin W C Chua H Hasan S A Hassan Z Z Deris Source Type: research