Bacillus amyloliquefaciens ALB65 Inhibits the Growth of Listeria monocytogenes on Intact Cantaloupe Melons.
In this study, we created a library of over 8,000 plant phyllosphere associated bacteria (PPAB) and screened them for the ability to inhibit the growth of L. monocytogenes in an in vitro fluorescence based assay. One isolate, later identified as Bacillus amyloliquefaciens ALB65, was able to inhibit the fluorescence of L. monocytogenes by > 30-fold in vitro B. amyloliquefaciens ALB65 was also able to grow, persist and reduce the growth of L. monocytogenes by > 1.5 log CFU on cantaloupe melon rinds inoculated with 5×103 CFU at 30°C, and was able to completely inhibit its growth at temperatures below 8°C. DNA sequenc...
Source: Applied and Environmental Microbiology - October 23, 2020 Category: Microbiology Authors: Tran TD, Del Cid C, Hnasko R, Gorski L, McGarvey JA Tags: Appl Environ Microbiol Source Type: research
Microbiological safety of ready-to-eat fresh-cut fruits and vegetables sold on the Canadian retail market.
Abstract
Following implementation of Health Canada's Policy on Listeria monocytogenes in Ready-to-Eat Foods by Canadian food safety authorities in 2011, a four-year study (2012-2016) was carried out to gain baseline information on the occurrence of bacterial pathogens, notably the prevalence and levels of Listeria monocytogenes (L. monocytogenes) in various product types of ready-to-eat (RTE) fresh-cut fruits and fresh-cut vegetables sold at retail in Canada. A total of 10,070 pre-packaged samples, including 4691 fresh-cut fruit and 5379 fresh-cut vegetable samples were collected from retail stores across ...
Source: International Journal of Food Microbiology - September 3, 2020 Category: Food Science Authors: Zhang H, Yamamoto E, Murphy J, Locas A Tags: Int J Food Microbiol Source Type: research
Detection and characterisation of Salmonella enterica serovar Hvittingfoss in Bar-tailed Godwits (Limosa lapponica) from Roebuck Bay, north-western Australia.
Abstract
Salmonella enterica serovar Hvittingfoss is an important foodborne serotype of Salmonella, being detected in many countries where surveillance is conducted. Outbreaks can occur, including a recent multi-state foodborne outbreak in Australia. S Hvittingfoss can be found in animal populations, though a definitive animal host has not been established. Six species of birds were sampled at Roebuck Bay, a designated Ramsar site in north-western Australia, resulting in 326 cloacal swabs for bacterial culture. A single flock of 63 Bar-tailed Godwit (Limosa lapponica menzbieri) caught at Wader Spit, Roebuc...
Source: Applied and Environmental Microbiology - July 30, 2020 Category: Microbiology Authors: Smith HG, Bean DC, Hawkey J, Clarke RH, Loyn R, Larkins JA, Hassell C, Valcanis M, Pitchers W, Greenhill AR Tags: Appl Environ Microbiol Source Type: research
Viruses, Vol. 12, Pages 477: Transport of Phage in Melon Plants and Inhibition of Progression of Bacterial Fruit Blotch
hoi
Bacterial fruit blotch (BFB) is an economically important disease in melons and watermelons for which no effective control method is available. Application of phytobacterium-infecting phage has been evaluated as an alternative means of preventing bacterial diseases in plants. Coating of seeds with bacteriophages infecting Acidovorax citrulli, the causal agent of BFB, is effective for controlling the disease, as shown in our previous study. We evaluated the transport of bacteriophage ACPWH from soil to the leaves of melon plants, and we also evaluated its effect on BFB. Leaves of melon plants were spray-inoculated w...
Source: Viruses - April 22, 2020 Category: Virology Authors: Aryan Rahimi-Midani Tae-Jin Choi Tags: Article Source Type: research
Cooperative crystallization effect in the formation of sonochemically grafted active materials based on polysaccharides.
Abstract
The current study explores the formation of active eco-friendly materials capable of preventing microbial contamination using in situ ultrasonic grafting of vanillin, curcumin and a curcumin-vanillin mixture on the surfaces of carboxymethylcellulose (CMC) and chitosan films. Spectroscopic, microscopic, physical and mechanical studies revealed that the films grafted with curcumin-vanillin mixture demonstrate improved mechanical properties and higher degree of order. The bioactivity of the prepared films was tested on food model, fresh-cut melons and films with a deposited curcumin-vanillin mixture ...
Source: Colloids and Surfaces - March 2, 2020 Category: Biotechnology Authors: Shebis Y, Kumar VB, Gedanken A, Poverenov E Tags: Colloids Surf B Biointerfaces Source Type: research
Sensors, Vol. 19, Pages 5010: Standard Analytical Methods, Sensory Evaluation, NIRS and Electronic Tongue for Sensing Taste Attributes of Different Melon Varieties
Kokai
Kappel
Grafting by vegetables is a practice with many benefits, but also with some unknown influences on the chemical composition of the fruits. Our goal was to assess the effects of grafting and storage on the extracted juice of four orange-fleshed Cantaloupe type (Celestial, Donatello, Centro, Jannet) melons and two green-fleshed Galia types (Aikido, London), using sensory profile analysis and analytical instruments: An electronic tongue (E-tongue) and near-infrared spectroscopy (NIRS). Both instruments are known for rapid qualitative and quantitative food analysis. Linear discriminant analysis (LDA) was u...
Source: Sensors - November 15, 2019 Category: Biotechnology Authors: N émeth Balazs Daood Kovacs Bodor Zaukuu Szentpeteri Kokai Kappel Tags: Article Source Type: research
Observational assessment of food safety behaviours at farmers’ markets in Ontario, Canada: A cross-sectional study
Publication date: Available online 4 September 2019Source: Food ControlAuthor(s): Ian Young, Aeri Chung, Jennifer McWhirter, Andrew PapadopoulosAbstractFarmers' markets are increasingly popular food venues in Canada. However, they have unique food safety challenges due to their operational characteristics. The purpose of this study was to evaluate the food safety behaviours of farmers' market vendors in the Greater Toronto Area of Ontario, Canada, to identify possible food safety risks and areas for future education and training. The food safety behaviours of vendors selling time-temperature control for safety (TCS) foods ...
Source: Food Control - September 4, 2019 Category: Food Science Source Type: research
The first two complete mitochondrial genome of Dacus bivittatus and Dacus ciliatus (Diptera: Tephritidae) by next-generation sequencing and implications for the higher phylogeny of Tephritidae.
In this study, we sequenced the complete mitochondrial genomes of the above species which are representatives of two subgenera of Dacus (Dacus and Didacus) not previously sequenced and reconstructed the phylogeny of Tephritidae. The mitochondrial genomes of D. bivittatus and D. ciliatus were 15,833 bp and 15,808 bp in length, respectively. The 37 genes, including 13 protein-coding genes (PCGs), 2 rRNA genes and 22 tRNA genes, with a long non-coding region (A + T-rich control region) were in the same arrangement as the ancestral insect mitochondrial genome. Phylogenetic analysis showed that Dacus has a closer relati...
Source: International Journal of Biological Macromolecules - August 8, 2019 Category: Biochemistry Authors: Zhang Y, Feng S, Fekrat L, Jiang F, Khathutshelo M, Li Z Tags: Int J Biol Macromol Source Type: research
Identification of chemotypes in bitter melon by metabolomics: a plant with potential benefit for management of diabetes in traditional Chinese medicine
ConclusionOur finding revealed a clear chemotype distribution of bioactive components across bitter melon varieties. While bioactivities of individual CTGs and OTGs still need to be investigated in more depth, our results could help in future the selection of bitter melon varieties with optimised metabolites profile for an improved management of diabetes with this popular edible Chinese folk medicine. (Source: Metabolomics)
Source: Metabolomics - July 17, 2019 Category: Biology Source Type: research
Molecules, Vol. 24, Pages 2374: Curcumin-Based Photodynamic Sterilization for Preservation of Fresh-Cut Hami Melon
This study aimed at investigating the influence of two key parameters (concentration of curcumin and illumination time) on the effects of curcumin-based photodynamic sterilization on the preservation of fresh-cut Hami melons. The results indicated that illumination with 50 μmol/L curcumin for 60 min using a blue LED lamp reduced the total aerobic microorganism count by ~1.8 log CFU/g in fresh-cut Hami melons. Besides this, the effects of photodynamic sterilization on the soluble solids content, color, water content, firmness, and sensory indices of the fresh-cut Hami melons were also evaluated. Compared to the c...
Source: Molecules - June 26, 2019 Category: Chemistry Authors: Yilin Lin Jiamiao Hu Shiyang Li Siti Sarah Hamzah Huiqin Jiang Arong Zhou Shaoxiao Zeng Shaoling Lin Tags: Article Source Type: research
Antimicrobial activity of cinnamon oil nanoemulsion against Listeria monocytogenes and Salmonella spp. on melons
Publication date: August 2019Source: LWT, Volume 111Author(s): Sumit Kumar Paudel, Kanika Bhargava, Hari KotturiAbstractListeria and Salmonella related recalls and outbreaks are of major concern to the melon industry. Cinnamon oil has shown its usefulness in antimicrobial food treatment. However, its applications are limited due to poor solubility of cinnamon oil in water. Utilization of Cinnamon oil nanoemulsion may offer effective antimicrobial treatment to the melon industry. Different formulations of cinnamon oil nanoemulsion were made by ultrasonication using Tween 80 as an emulsifier. The particle size of emulsion wa...
Source: LWT Food Science and Technology - May 25, 2019 Category: Food Science Source Type: research
Antioxidant and antidiabetic properties of Chinese and Indian bitter melons (Momordica charantia L.)
This study has comparatively evaluated the effects of two processing methods (fresh and freeze-drying) on Chinese and Indian BM by measuring their bioactivity in terms of total phenolic content (TPC), total triterpene content (TTC), antioxidant activity, and antidiabetic properties using the DPPH free radical scavenging and reducing power assays, and the α-amylase and α-glucosidase inhibition assays. The TPC (GAE mg/g dw) in freeze-dried BM were 6.03 and 6.09, and in fresh BM were 4.81 and 4.83 for Indian and Chinese BM, respectively. The TTC (OAE mg/g dw) in Indian BM were 7.25 and 5.63, and in Chinese BM were 5.88 and ...
Source: Food Bioscience - April 2, 2019 Category: Food Science Source Type: research
Development of flexible antimicrobial zein coatings with essential oils for the inhibition of critical pathogens on the surface of whole fruits: Test of coatings on inoculated melons
This study aimed to develop essential oil (EO)-containing antimicrobial coatings for the inhibition of pathogenic bacteria contamination on fruit peels. Incorporation of eugenol (EUG), carvacrol (CAR), and thymol (THY) into films at ≥ 1% (w/w) eliminated the typical brittleness of zein films. However, EUG outperformed CAR and THY in terms of mechanical properties. Films with ≥ 2% (w/w) CAR and THY and ≥ 3% EUG showed clear zones against L. innocua and E. coli in agar medium at 37 °C. All EO-containing films also inhibited L. innocua and E. coli inoculated at their surfaces by minimum 3.9 and 2.7 decimal (D) within...
Source: Food Packaging and Shelf Life - March 27, 2019 Category: Food Science Source Type: research
Microsatellite Markers Reveal Genetic Diversity and Relationships within a Melon Collection Mainly Comprising Asian Cultivated and Wild Germplasms.
In this study, thick-skinned (TC) (cultivated subspecies melo), thin-skinned (TN) (cultivated subspecies agrestis), and wild accessions were analyzed for genetic diversity and relationships using 36 microsatellite markers. A total of 314 alleles were detected with a mean allelic number of 8.72 and polymorphism information content of 0.67. Cluster analysis of the accessions resulted in four distinct clusters (I, II, III, and IV) broadly matching with the TC, TN, and wild groups. Cluster I contained only two Indian wild accessions. Cluster II was consisted of 49 South Asian accessions, 34 wild accessions, and 15 TN accession...
Source: Biomed Res - March 21, 2019 Category: Research Authors: Hu J, Gao L, Xu Y, Li Q, Zhu H, Yang L, Li J, Sun S Tags: Biomed Res Int Source Type: research
Effects of Penicillium infection on the expression and activity of CDPK2 in postharvest Hami melon treated with calcium chloride
Publication date: Available online 16 February 2019Source: Physiological and Molecular Plant PathologyAuthor(s): Ming Ning, Fengxian Tang, Qin Zhang, Xinxin Zhao, Liping Yang, Wenchao Cai, Chunhui ShanAbstractCalcium ions are second messengers in plant signal transduction involved in plant growth and development and the response to stress. Calcium-dependent protein kinase (CDPK) is calcium ion receptor functioning in plant disease resistance. Herein, we investigated changes in CDPK activity and resistance to Penicillium in postharvest Hami melons after different treatments. The non-infection group was permeated with calciu...
Source: Physiological and Molecular Plant Pathology - February 17, 2019 Category: Molecular Biology Source Type: research
