Development of recombinant capsid protein-based single serum dilution ELISA for sero-detection of porcine circovirus type 2 infection in pigs
In this study, a recombinant capsid protein-based single serum dilution indirect ELISA was developed for determination of antibody titre of the infected pigs. The capsid protein (Cap) of PCV2d was produced inSaccharomyces cerevisiae cells and the capsid protein was purified by affinity chromatography. This recombinant protein was used as a coating antigen to develop a cost effective, highly sensitive and specific single serum dilution ELISA. The in-house developed ELISA was optimized to be used in a 1:200 single serum dilution. The developed ELISA along with a commercial ELISA kit were compared with a sensitive immuno-pero...
Source: Veterinary Research Communications - January 12, 2024 Category: Veterinary Research Source Type: research
Liver and kidney damage in quail embryos hatched from eggs sprayed once with chlorpyrifos during artificial incubation
This study aimed to assess the potential impacts of CPF on common quail (Coturnix coturnix) embryos through external eggshell application during key embryonic stages (days 1, 4, and 14). Concentrations equivalent to those used in fruit applications, 5 × and 10 × (38.4, 192, and 384 µg/egg), were employed. A 48% commercial formulation of CPF (Pirfos - Icona®) was utilized. An increase in embryonic deaths, as well as a statistical association between the degree of liver or kidney lesions and CPF concentrations was observed. The results sug gest that CPF can induce embryotoxic effects with a single exposure to quail ...
Source: Veterinary Research Communications - January 11, 2024 Category: Veterinary Research Source Type: research
First serological diagnosis of Mycobacterium avium subsp. paratuberculosis infection in sheep in the state of Pernambuco, Brazil
This study aimed to diagnoseMycobacterium avium subsp.paratuberculosis (MAP) infections in sheep in the state of Pernambuco, Brazil. A total of 276 blood samples were analyzed using the enzyme-linked immunosorbent assay IDEXX Paratuberculosis Screening kit, and 261 fecal samples were submitted for bacterial culture and polymerase chain reaction tests. An animal-level sero-frequency of 0.72% (n = 2/276) and a farm-level sero-frequency of 20% (n = 2/10) were found. All fecal sample cultures were negative, and molecular analyses were also negative. To the best of our knowledge, this is the first study of MAP infecti...
Source: Veterinary Research Communications - January 11, 2024 Category: Veterinary Research Source Type: research
Pre-fertilization approach using α-l-fucosidase modulates zona pellucida hardening during bovine in vitro embryo production
AbstractThe polyspermy occurrence is considerably lower underin vivo compared toin vitro embryo culture conditions, suggesting that the presence of some factors in the maternal environment is responsible for this. The α-L-fucosidase (FUCA) is a natural glycosidase present in the oviductal fluid, therefore, this study aimed at investigating the effect of adding FUCA to the hardening of the zona pellucida (ZP), polyspermy control, and embryonic yield and quality of bovine blastocysts producedin vitro. In the first experiment, the effect of FUCA (0.125 U/mL) was evaluated during the entirein vitro fertilization (IVF). Howeve...
Source: Veterinary Research Communications - January 8, 2024 Category: Veterinary Research Source Type: research
Survey on resistance occurrence for F4+ and F18+ enterotoxigenic Escherichia coli (ETEC) among pigs reared in Central Italy regions
AbstractPorcine Post Weaning Diarrhoea (PWD) is one of the most important swine disease worldwide, caused by EnterotoxigenicEscherichia coli (ETEC) strains able to provoke management, welfare and sanitary issues. ETEC is determined by proteinaceous surface appendages. Numerous studies conducted by now in pigs have demonstrated, at the enterocytes level, that, the genesmucin 4 (MUC4) andfucosyltransferase (FUT1), coding for ETEC F4 and F18 receptors respectively, can be carriers of single nucleotide polymorphisms (SNPs) associated with natural resistance/susceptibility to PWD. The latter aspect was investigated in this stud...
Source: Veterinary Research Communications - January 4, 2024 Category: Veterinary Research Source Type: research
Recombinantly expressed virus-like particles (VLPs) of canine circovirus for development of an indirect ELISA
In this study, full length CanineCV capsid gene was expressed inEscherichia coli followed by two-step purification process to yield soluble capsid protein in high concentration. Transmission electron microscopy (TEM) confirmed the capsid antigen self-assembled into 17 –20 nm VLPs in glutathione S-transferase (GST) buffer, later utilised to develop an indirect enzyme-linked immunosorbent assay (iELISA). The respective sensitivity and specificity of the proposed iELISA were 94.10% and 88.40% compared with those obtained from Western blot. The mean OD450 value for western blot positive samples was 1.22 (range 0.12 –3.39)...
Source: Veterinary Research Communications - January 2, 2024 Category: Veterinary Research Source Type: research
CD46 inhibits the replication of swine influenza viruses by promoting the production of type I IFNs in PK-15 cells
AbstractSwine flu caused by swine influenza A virus (swIAV) is an acute respiratory viral disease that is spreading in swine herds worldwide. Although the effect of some host factors on replication of swIAV has been identified, the role of CD46 in this process is unclear. Here, we report that CD46 inhibits the replication of swIAV by promoting the production of type I interferons (IFNs) in porcine kidney (PK-15) cells. CD46 knockout (CD46-KO) and stably expressing (CD46-overexpression) PK-15 cells were prepared using lentivirus-mediated CRISPR/Cas9 gene editing and seamless cloning technology. The results of virus infectio...
Source: Veterinary Research Communications - December 28, 2023 Category: Veterinary Research Source Type: research
Therapeutic management of osteodystrophia fibrosa in early weaned Malabari male goat kids
This report describes the successful therapeutic management of acute osteodystrophia fibrosa in early-weaned goat kids by following a medical protocol. Three Malabari male goat kids of forty days old from two different litters of the same farm were presented with spontaneous, bilaterally symmetrical, non-inflammatory facial swelling, protruded tongue, prominent eyeballs, frothy drooling saliva, diarrhoea, and inanition due to the inability in mastication or suckling for the last two weeks. As per the history, these kids were weaned and started feeding pelleted concentrate feed and jackfruit leaves along with the milk sucki...
Source: Veterinary Research Communications - December 22, 2023 Category: Veterinary Research Source Type: research
Body condition scoring in alpacas (Vicugna pacos) and llamas (Lama glama) – a scoping review
AbstractAssessment of the body condition score (BCS) is an important tool to check the nutritional status of an animal, and thus can provide important information in herd management, but also in the veterinary examination of individual animals. In dairy farming, BCS has been recognized as an important parameter for a long time already, but over the years body condition scoring has also become more important for other species. Especially in South American camelids (SACs: alpacas and llamas), whose dense coat can hide a poor nutritional status for a long time, regular assessment of the BCS is recommended by different authors...
Source: Veterinary Research Communications - December 22, 2023 Category: Veterinary Research Source Type: research
Procalcitonin and carbonylated protein concentrations in equine synovial fluid
This study evaluated PCT and PCC in plasma and synovial fluid (SF), in healthy and pathological equine joints, affected by different types of arthropathy. Twenty-nine joints were evaluated and underwent orthopedic, radiographic, ultrasonographic and SF evaluation. The joints were divided in three groups: healthy, septic, and non-septic arthritis. PCT and PCC were measured in horse plasma and SF. Data distribution was evaluated and results were expressed as median, quartile values. Statistical differences in SF values among groups and correlations were assessed between plasma and SF of both PCT and PCC. The groups of joint ...
Source: Veterinary Research Communications - December 21, 2023 Category: Veterinary Research Source Type: research
Circulation of picobirnavirus in Neotropical free-ranging mammals
The objective of this study was to expand our knowledge of the circulation of PBV in free-living animals from two regions (Brazil and Argentina) of the Atlantic Forest. Fecal samples were analyzed from free-living animals: tapir, brocket deer, peccary, and different species of rodents and marsupials. A total of 133 samples were collected and analyzed by RT-PCR, of which 44 (33.08%) were PBV-positive. Nine amplicons were sequenced, five species from Argentina and four from Brazil, and phylogenetic analysis was performed. The nucleotide and amino acid identities of the PBV strains detected in animals from Argentina and Brazi...
Source: Veterinary Research Communications - December 19, 2023 Category: Veterinary Research Source Type: research
Pulmonary acinar adenocarcinoma in a captive lioness (Panthera leo)
This study aimed to describe the clinical and pathological aspects of a case of diffuse pulmonary acinar adenocarcinoma in a 23-year-old, captive lioness with clinical history of dyspnea, progressive weight loss and inappetence. At necropsy, the lungs were mildly pale, moderately firm, and the pleural surface was diffusely irregular with multifocal to coalescent, grey to white areas. No masses or superficial nodules were detected, but, on the cut surface, there were numerous, spherical, firm, white to yellow areas up to 0.5 cm in diameter affecting all pulmonary lobes. Histologically, in the lungs, there were extensive, ...
Source: Veterinary Research Communications - December 19, 2023 Category: Veterinary Research Source Type: research
Correction to: The effect of enterocin A/P dipeptide on growth performance, glutathione-peroxidase activity, IgA secretion and jejunal morphology in rabbits after experimental methicillin-resistant Staphylococcus epidermidis P3Tr2a infection
(Source: Veterinary Research Communications)
Source: Veterinary Research Communications - December 19, 2023 Category: Veterinary Research Source Type: research
Nexus between prevalence of Taenia solium (Cysticercus cellulosae) in pigs and retrospective study of humans cysticercosis cases in Benin hospitals
This study was carried out to determine the prevalence of porcine cysticercosis and to identify potential risk factors associated and their seroprevalence as well in Benin. The tongue palpation was performed on 4150 pigs from the pig farmers were randomly selected to diagnose cysticercosis and to assess the risk factors associated. In slaughterhouses, 792 pigs were inspected. Serum samples from 460 pigs were tested of the presence ofTaenia solium cysticercosis using the HP10 antigen-ELISA (Ag-ELISA). A logistic regression model with a 95% confidence interval was used to analyse the main risk factors associated. The tongue ...
Source: Veterinary Research Communications - December 18, 2023 Category: Veterinary Research Source Type: research
Haematology and biochemistry in healthy llamas at sea level
In conclusion, different ambient and methodological c onditions might affect some parameters. The parameters hereby presented are representative of llama’s population living at sea level in South America. (Source: Veterinary Research Communications)
Source: Veterinary Research Communications - December 18, 2023 Category: Veterinary Research Source Type: research
