Comprehensive review of resveratrol as a feed additive in dairy cows: exploring its potential diverse effects and implications
In conclusion, RSV polyphenol at optimal doses is a promising agent for the prevention and treatment of metabolic abnormalities in dairy cows, based on our literature review and study results. (Source: Veterinary Research Communications)
Source: Veterinary Research Communications - July 8, 2023 Category: Veterinary Research Source Type: research

Development, characterization, and immunomodulation performance of spray-dried Moringa oleifera seed extract in Longfin yellowtail Seriola rivoliana
AbstractMoringa oleifera is one of the most promising plants in aquaculture because it improves the health status, zootechnical parameters and resistance against diseases. This research evaluates the physicochemical, antioxidant values of spray-driedMoringa oleifera seed extract microencapsulates obtained at 140 and 180  °C with whey protein concentrate (WPC) and maltodextrin (MD) as wall materials in two different proportions: WPC 100% and WPC-MD (3:1). Also, immune response of peripheral blood leukocytes (PBL) of Longfin yellowtailSeriola rivoliana stimulated with spray-driedMoringa oleifera seed for 24  h was assesse...
Source: Veterinary Research Communications - July 7, 2023 Category: Veterinary Research Source Type: research

Exposure to copper induces endoplasmic reticulum (ER) stress-mediated apoptosis in chicken (Gallus gallus) myocardium
AbstractCopper (Cu), an omnipresent environmental pollutant, can cause potential harm to the public and ecosystems. In order to study the cardiotoxicity caused by Cu, molecular biology techniques were used to analyze the effect of Cu on ER stress-mediated cardiac apoptosis. Invivo investigation, 240 1-day-old chickens were fed with Cu (11, 110, 220, and 330  mg/kg) diet for 7 weeks. The consequence showed that high-Cu can induce ER stress and apoptosis in heart tissue. Thevitro experiments, the Cu treatment for 24  h could provoke ultrastructural damage and upregulate the apoptosis rate. Meanwhile, GRP78, GRP94, eIF2α, ...
Source: Veterinary Research Communications - July 5, 2023 Category: Veterinary Research Source Type: research

Effect of replacing inorganic iron with iron-rich microbial preparations on growth performance, serum parameters and iron metabolism of weaned piglets
This study aimed to investigate the effects of replacing of dietary inorganic iron with iron-richLactobacillus plantarum and iron-richCandida utilis on the growth performance, serum parameters, immune function and iron metabolism of weaned piglets. Fifty-four 28-day-old healthy Duroc × Landrace × Yorkshire castrated male weanling piglets of similar body weight were randomly and equally divided into three groups. The piglets were kept in three pens per group, with six pigs in each pen. The dietary treatments were (1) a basal diet + ferrous sulfate preparation containing 120  mg/kg iron (CON); (2) a basal diet + i...
Source: Veterinary Research Communications - July 4, 2023 Category: Veterinary Research Source Type: research

Short communications: an ear-attached accelerometer detects effects of regrouping on lying, rumination, and activity times in calves
The objective of this study was to use a sensor-based accelerometer (ACC) to identify changes in lying, rumination, and activity times in weaned calves during the moving and regrouping process. Overall, 270 healthy Holstein calves (from approximately 16 regrouping events) at the age of approximately 4 months were enrolled and equipped with an ear-attached ACC (SMARTBOW, Smartbow GmbH/ Zoetis LLC). Sensor data were recorded for 5 d before (d -5) until 4 d after moving and regrouping (d 4). The day of regrouping was defined as d 0. Acceleration data were continuously processed by specific algorithms (developed by SMARTBOW) f...
Source: Veterinary Research Communications - July 1, 2023 Category: Veterinary Research Source Type: research

Biological properties and diverse cytokine profiles followed by in vitro and in vivo infections with LSDV strain isolated in first outbreaks in Vietnam
AbstractPreliminary information about LSD virus isolated from the first outbreaks in Vietnam has been reported by our laboratory. In the current study, LSDV strain, LSDV/Vietnam/Langson/HL01(HL01) was further analyzed to provide a better understanding of this viral pathogen. HL01 LSDV strain was propagated at MOI 0.01 in MDBK cells and then given to cattle at dose of 106.5 TCID50/ml (2ml/animal). The production of proinflammatory (IFN- γ, IL-1α, and TNF-α) and anti-inflammatory (IL-6, IL-10, and TGF-ß1) cytokines were measured by real-time PCR, both In vitro and In vivo. The results demonstrated that HL01 strain caused...
Source: Veterinary Research Communications - June 29, 2023 Category: Veterinary Research Source Type: research

Mapping of dressed and processed poultry products in Bangladesh: Identifying the food safety risks for policy intervention
AbstractBangladesh ’s commercial poultry production is growing rapidly, including the commercial processing of poultry. This expansion of poultry processing plants is fueled by the belief that this sub-sector provides safer food and has less food-borne disease risks compared to traditional live bird markets (LBMs). The purpose of this study is to describe Bangladesh’s dressed and processed poultry production and distribution network (PDN), identify what and where quality control occurs, and suggest where improvements could be made. Engaging with PDN for dressed and processed poultry, we used in-depth inter views with...
Source: Veterinary Research Communications - June 27, 2023 Category: Veterinary Research Source Type: research

Modulation of immune genes in the mucosal-associated lymphoid tissues of cobia by Sarcodia suae extract
AbstractRachycentron canadum (cobia) is a marine fish species of high economic value in aquaculture due to its fast growth rate and good feed conversion efficacy. Regrettably, the industry has been affected by significant setbacks from high mortality due to diseases. Consequently, an improved perception of innate immunity correlated to each mucosal-associated lymphoid tissue (MALT) in teleost fish is necessary to understand hosts ’ response towards infections better. The utilization of polysaccharides in seaweed to stimulate the immune system has gathered unprecedented attention. The present study examined the immunostim...
Source: Veterinary Research Communications - June 23, 2023 Category: Veterinary Research Source Type: research

A study of the inter- and intra-operator variability on selected echocardiographic measurements in dogs
AbstractStandard transthoracic echocardiography is considered the non-invasive gold standard for the diagnosis of most cardiac diseases. Defining reproducibility, repeatability, and reliability of this exam is imperative to reduce errors in clinical evaluations. The present study aimed at: (1) evaluating the reproducibility and repeatability of 15 echocardiographic parameters in dogs by analyzing measurements obtained from several operators with different levels of experience and comparing them to the ones obtained from two board-certified operators (gold standards - GSs); (2) assessing whether different formative paths ha...
Source: Veterinary Research Communications - June 23, 2023 Category: Veterinary Research Source Type: research

Fosfomycin modifies the replication kinetics of bovine alphaherpesvirus-1 and reduces the timing of its protein expression on bovine (MDBK) and human (SH-SY5Y) cell lines
AbstractBovine alphaherpesvirus 1 (BoAHV-1) predisposes cattle to respiratory secondary bacterial infections, which can be treated with the broad-spectrum antibiotic fosfomycin. This drug also suppresses NF-kB activity and pro-inflammatory responses. Therefore, cattle may be exposed to an interaction between the virus and the antibiotic which may have effects on it. The aim of this study was to determine the effect of calcium fosfomycin (580  µg/mL) on BoAHV-1 (moi = 0.1) replication. Two cell lines (MDBK and SH-SY5Y) were used in this study. Our results show that fosfomycin has novel properties. By MTT assay we have...
Source: Veterinary Research Communications - June 17, 2023 Category: Veterinary Research Source Type: research

Assessing failure of transfer of passive immunity by gamma-glutamyl-transferase activity and serum refractometry in holstein-friesian calves affected by neonatal diarrhea
This study evaluated the diagnostic performance and differences among optical serum total protein (STP) concentration and gamma-glutamyl-transferase (GGT) activity for assessing FTPI in diarrheic Holstein Friesian calves. 72 diarrheic and 19 healthy Holstein Friesian calves aged 1 to 10 days were enrolled. Each calf underwent a complete clinical examination and dehydration assessment. The effect of dehydration status and age on the correlation between the two methods under study (STP and GGT) and the gold standard (Immunoglobulin G [IgG] measured with RID) was investigated with Spearman ’s correlation index R for ranks. ...
Source: Veterinary Research Communications - June 14, 2023 Category: Veterinary Research Source Type: research

Turkey adenovirus 3: ORF1 gene sequence comparison between vaccine-like and field strains
In conclusion, the method employed in this study could be a useful tool towards making a correct diagnosis. The data could contribute to the knowledge of field distribution of THEV strains and increase the limited existing information available on native isolates around the world. (Source: Veterinary Research Communications)
Source: Veterinary Research Communications - June 8, 2023 Category: Veterinary Research Source Type: research

Pathological observation and transcriptomic analysis of thymus injury in PRRSV-infected piglets
This study was aimed at investigating the specific manifestations of thymus injury through the construction of a PRRSV-infected piglet model and histopathological observation. In this study, fourteen 40-day-old PRRSV-free piglets were randomly divided into two groups, eleven of which were intramuscularly injected with 3  mL of PRRSV WUH3 virus suspension (106 PFU /mL) in the infection group, and three of which were sham-inoculated with 3  mL of RPMI-1640 medium in the control group. Clinical necropsy and samples collection were performed on day 8 after artificial infection. With the Illumina platform, the transcriptomes ...
Source: Veterinary Research Communications - June 2, 2023 Category: Veterinary Research Source Type: research

Development of a multiplex quantitative PCR assay for simultaneous detection of Treponema phagedenis, Treponema pedis, Treponema medium, and ‘Treponema vincentii’ and evaluation on bovine digital dermatitis biopsies
AbstractBovine digital dermatitis (BDD) is a contagious foot disease with worldwide occurrence in dairy cattle. The disease causes lameness and reduced animal welfare as well as economic losses for the farmer. The aetiology is not fully established but associations have been made withTreponema spp. Today, BDD diagnosis is mainly based on visual inspection of cattle feet, therefore this study aimed to develop a multiplex quantitative PCR (qPCR) assay targetingTreponema phagedenis,Treponema pedis, Treponema medium, and‘Treponema vincentii’ to aid in diagnosis. The assay was tested for specificity on 53 bacterial strains ...
Source: Veterinary Research Communications - June 1, 2023 Category: Veterinary Research Source Type: research

Effect of different masses, ages, and coats on the thermoregulation of dogs before and after exercise across different seasons
In this study, we looked at thermoregulation in dogs of different sizes, ages, coat types, and head morphologies across three different seasons. We used tympanic membrane temperatures (Tear) and infrared thermography to observe temperature regulation in pet dogs before and after a 45-min moderate walking exercise trial. We hypothesized that Tear and heat dissipation is positively correlated with body mass. Using network analysis, we found that body mass was among the most central features for spring and summer trials, but not for the winter trials. Similarly, leg length, snout length, and paw width were the central predict...
Source: Veterinary Research Communications - May 25, 2023 Category: Veterinary Research Source Type: research