Pilot survey reveals ophidiomycosis in dice snakes Natrix tessellata from Lake Garda, Italy
AbstractOphidiomycosis is an emerging infectious disease caused by the fungusOphidiomyces ophidiicola (Oo). To date, Oo presence or associated disease condition has been recorded in wild and/or captive snakes from North America, Europe, Asia and Australia, but the data is still scarce outside the Nearctic. Although Italy is a country with a high snake biodiversity in the European panorama, and animals with clinical signs compatible with Oo infection have been documented, to date no investigations have reported the disease in the wild. Therefore, a pilot survey for the Italian territory was performed in conjunction with set...
Source: Veterinary Research Communications - April 29, 2023 Category: Veterinary Research Source Type: research
Biomarkers of oxidative stress and liver function in early lactation and their relationship with the reproductive efficiency of multiparous grazing dairy cows in Argentina. A retrospective study
This study aimed to analyze the possible relationship between days to conception and different oxidative stress (OS) biomarkers and liver functional parameters in multiparous dairy cows. Besides, a fast reliable method for the accurate measurement of malondialdehyde (MDA) by liquid chromatography –tandem mass spectrometry was developed in several matrices. During lactation, the days to conception of 28 cows were determined for a retrospective study. According to this parameter, cows were divided into two groups: high and low days to conception (HDC and LDC, respectively). Blood, urine and liver biopsies were sampled 21 ...
Source: Veterinary Research Communications - April 28, 2023 Category: Veterinary Research Source Type: research
Evaluation of the susceptibility of Tritrichomonas foetus to extracts of Lantana camara (Verbenaceae) by flow cytometry
AbstractBovine Trichomonosis (BT), a sexually transmitted disease endemic in countries with extensive cattle farming and natural service, is one of the most common causes of reproductive failure. 5-nitroimidazoles and their derivatives are used for its treatment, mainly metronidazole. The emergence of drug resistance mechanisms and treatment failures raise the need to investigate the effectiveness of new active compounds that contribute to parasite control. In this regard, extracts ofLantana camara (Verbenacea) have shown high biocidal potential against isolates ofTrypanosoma cruzi andLeishmania braziliensis in vitro assay...
Source: Veterinary Research Communications - April 27, 2023 Category: Veterinary Research Source Type: research
Isolation, characterization, and genome analysis of a broad host range Salmonella phage vB_SenS_TUMS_E4: a candidate bacteriophage for biocontrol
In this study, a lytic phage, vB_SenS_TUMS_E4 (E4), was isolated from poultry effluent and characterized to evaluate its potential and efficacy for bio-controllingS. enteritidis in foods. Transmission electron microscopy revealed that E4 has a siphovirus morphotype, with an isometric head and non-contractile tail. Determining the host range showed that this phage can effectively infect different motile as well as non-motileSalmonella enterica serovars. The biological characteristics of E4 showed that it has a short latent period of about 15 min and a large burst size of 287 PFU/cell, and is also significantly stable in a b...
Source: Veterinary Research Communications - April 25, 2023 Category: Veterinary Research Source Type: research
Clinical use of the canine foetal kidney formula in dogs of different maternal sizes during the last ten days before parturition
AbstractData concerning the use of the canine foetal kidney length (L) formula in the prediction of parturition timing are still lacking. In our study, we aimed to evaluate the accuracy of the L formula in predicting the parturition date during the last 10 days of pregnancy. Twenty-five clinically healthy pregnant bitches, aged 2 –9 years and weighing 3.5–52.2 kg, were ultrasonographically monitored from -11 to 0 days before parturition (dbp). Kidney L was measured for the three most caudal foetuses, and the parturition day was estimated using the kidney formula, whose accuracy was calculated as the percentage of case...
Source: Veterinary Research Communications - April 24, 2023 Category: Veterinary Research Source Type: research
First description of a Gammaherpesvirus in a common dolphin (Delphinus delphis) from the Eastern Mediterranean Sea
AbstractIn September 2020, a male common dolphin (Delphinus delphis) was found dead on a beach near Bat-Yam, Israel. A small, raised, well circumscribed penile lesion (i.e., mass) was identified and removed for histology and molecular characterizations. By histology, the penile mass presented focal keratinization of the squamous epithelium and a mild ballooning of acanthocytes in lower epithelium levels, as well as features compatible with viral plaques, and tested positive for a gammaherpesvirus through molecular characterization analyses. Tissue samples from the lungs, liver, and spleen, however, tested negative for herp...
Source: Veterinary Research Communications - April 24, 2023 Category: Veterinary Research Source Type: research
The first report of Setaria tundra (Issaitshikoff & amp; Rajewskaya, 1928) in Slovakia by using of molecular methods
This study is the first report ofS. tundra in Slovakia. (Source: Veterinary Research Communications)
Source: Veterinary Research Communications - April 22, 2023 Category: Veterinary Research Source Type: research
Novel Anaplasma (Rickettsiales: Anaplasmataceae) strain and Hepatozoon sp. cf. H. procyonis (Apicomplexa, Hepatozoidae) detected in Procyon cancrivorus (Carnivora, Procyonidae) from Argentina, with note of tick-host association
AbstractThe aim of this work is to report the first detection ofProcyon cancrivorus naturally co-infected withHepatozoon sp. cf.H. procyonis and a novelAnaplasma strain from South America and potential vector tick species associated. On August 30, 2016, a specimen ofP. cancrivorus was found dead on the route in Chaco province, Argentina. A tick and a blood sample by cardiac puncture was collected from the specimen. DNA was extracted from blood sample and the tick was morphological identity as a female ofAmblyomma ovale. Molecular detection of Anaplasmataceae family andHepatozoon spp. agents was performed targeting two diff...
Source: Veterinary Research Communications - April 21, 2023 Category: Veterinary Research Source Type: research
Distribution of virulence-associated genes, antibiotic resistance and phylogenetic groups in Escherichia coli isolated from domestic and racing pigeons
AbstractDespite a lot of information about virulence and resistance ofEscherichia coli (E. coli) in poultry, very limited data are currently available on its occurrence in pigeon isolates, although this poses a threat to human and animal health. Therefore, this study was conducted to explore the phylogenetic classification, antibiotic sensitivity, and virulence factors inE. coli isolated from cloacal swabs of domestic pigeons bred for meat (n = 47) and racing pigeons (n = 44). The most frequent phylogroup in racing pigeons was E (36, 82.00%), unlike domestic pigeons (B2- 19, 40.00%). The most abundant iron uptake ...
Source: Veterinary Research Communications - April 20, 2023 Category: Veterinary Research Source Type: research
Production of recombinant proteins including the B-cell epitopes of autolysin A of Staphylococcus aureus isolated from clinical sheep mastitis and their potential for vaccine development
AbstractStaphylococcus aureus is the most common clinical mastitis-associated pathogen in sheep which contributes to reduced welfare of affected animals and, therefore, compromises the quality and quantity of milk production. To prevent mastitis and its spread, it is essential to guarantee adequate breeding conditions and animal health, through the adoption of good farm management practices and the application of suitable biosecurity measures. Vaccination can play a strategic role in prevention, control, and eradication of diseases. The identification of secreted and cellular antigens of the predominant sheep-CC130/ST700/t...
Source: Veterinary Research Communications - April 19, 2023 Category: Veterinary Research Source Type: research
Survival of Streptococcus equi subsp. zooepidemicus on environmental samples is affected by material type and incubation temperature
AbstractStreptococcus equi subsp.zooepidemicus is an opportunistic pathogen associated with disease in a range of domestic and wild animals. Despite its importance, very limited data is available on its survival and persistence on the environment. The goal of this study was to evaluate survival ofS. zooepidemicus under ideal culture conditions and farm-like setting, in various surface types. Rubber, plastic, wood, and concrete samples were sterilized and inoculated with 109 CFU/mL ofS. zooepidemicus with or without feces, and cultured under ideal conditions (37 °C, 5% CO2) or farm-like settings (20oC on air) for a maxim...
Source: Veterinary Research Communications - April 17, 2023 Category: Veterinary Research Source Type: research
Canine umbilical cord tissue derived mesenchymal stem cells naturally express mRNAs of some antimicrobial peptides
AbstractAntimicrobial peptides (AMPs) are naturally produced by all living organisms at a constitutive rate. They represent the first line of active defence systems against invading microorganisms, helping in innate immunity. Besides their therapeutic applications, great attention has also been given to the mesenchymal stem cells (MSCs) due to their antimicrobial activities. The study aimed to observe the mRNA expression profile of few antimicrobial peptides (AMPs) in canine MSCs during standardin vitro culture. MSCs were isolated from canine umbilical cord tissue, propagated and characterized by morphology, surface marker...
Source: Veterinary Research Communications - April 15, 2023 Category: Veterinary Research Source Type: research
Genetic haplotypes associated with immune response to Leishmania infantum infection in dogs
AbstractLeishmaniasis is a zoonotic parasitic disease, and the main reservoir of the parasite is the dog, although recent years have seen an increase in other mammalian species. In the Mediterranean region, where it is an endemic disease, it is caused by the speciesLeishmania infantum. The Ibizan hound, an autochthonous breed of this region, appears to have a genetic resistance to parasitic infection, whereas other canine breeds, such as the Boxer, are susceptible to infection. These differences are related to the differentiated activation of the immune response, with the Ibizan hound activating the Th1 immune response, wh...
Source: Veterinary Research Communications - April 14, 2023 Category: Veterinary Research Source Type: research
Lipid metabolites, interleukin-6 and oxidative stress markers in follicular fluid and their association with serum concentrations in mares
This study aimed to investigate associations between systemic and FF concentrations of interleukin-6 (IL-6), total cholesterol, triglycerides, non-esterified fatty acids (NEFA), reactive oxygen metabolites (d-ROMs), biological antioxidant potential (BAP), and oxidative stress index (OSI) during the non-breeding season in mares. At the slaughterhouse, serum and FF of small (5 –10 mm in diameter), medium (> 10–20 mm in diameter), and large (> 20–30 mm in diameter) follicles were sampled from 12 healthy mares. There was a strong positive association (P < 0.01) between the concentration of IL-6 in ...
Source: Veterinary Research Communications - April 14, 2023 Category: Veterinary Research Source Type: research
Animal models of Soft Tissue Sarcoma for alternative anticancer therapy studies: characterization of the A-72 Canine Cell Line
AbstractCanine Soft Tissue Sarcoma (STS) cell line A-72 has been largely employed for antiviral and antiproliferative studies. However, there are few information on their characteristics. Our aim was to evaluate A-72 expression level of genes and proteins involved in the innate immune response and cell cycle, their ability to respond to infective stressors and their possible use as a cellular model for anti-cancer studies in human and animal medicine. For this purpose, we evaluated the basal expression of immune-related, cell cycle and DNA repair genes on this cell line and tumoral tissues. A-72 ability to respond to a wil...
Source: Veterinary Research Communications - April 11, 2023 Category: Veterinary Research Source Type: research
