In vitro effects of uncarboxylated osteocalcin on buffalo Leydig cell steroidogenesis
AbstractUncarboxylated osteocalcin (UcOCN), a bone derived circulating protein, has been demonstrated to influence steroidogenesis in testicular Leydig cells of murine and human species. However, the role of UcOCN in testosterone biosynthesis remains unexplored in domestic animals. The present study aimed to investigate the impact of UcOCN on the expressions of steroidogenic genes (HSD3 β1, HSD3β6, CYP17A1, CYP11A1), testosterone production and GPRC6A receptor localization in buffalo Leydig cells. Leydig cells from the testes of adult Murrah buffalo were isolated, with an average cell count and viability after digestion ...
Source: Veterinary Research Communications - February 2, 2024 Category: Veterinary Research Source Type: research
Transcription levels of hes and their involvement in the biofilm formation of Shiga toxin-producing Escherichia coli O91
This study is the first to report the relationship between biofilm formation, andhes expression and proposes that the analysis and diagnosis of LAA, especiallyhes as STEC O91 virulence factors, could elucidate these unknown mechanisms. Considering that there is no specific treatment for HUS, only supportive care, it is necessary to know the survival and virulence mechanisms of STEC O91. (Source: Veterinary Research Communications)
Source: Veterinary Research Communications - January 24, 2024 Category: Veterinary Research Source Type: research
Bovine TB serological diagnosis: blindness rather than conundrum
(Source: Veterinary Research Communications)
Source: Veterinary Research Communications - January 24, 2024 Category: Veterinary Research Source Type: research
Effects of temperament during handling and social rank on the blood biochemical parameters of common eland (Taurotragus oryx)
AbstractLarge herbivores are subject to handling and social stress in captivity. These may affect blood biochemical values, which motivated this research. Twelve healthy common eland (Taurotragus oryx) were monitored for 12 months. The animals were handled monthly, and blood samples were collected. Samples from every second month were analysed for 14 blood biochemical parameters. Temperament throughout the handling, as the summation of various behavioural responses, was calculated as a proxy of the stress generated during handling. Social behaviour was recorded each month, and the agonistic interactions were used to calcul...
Source: Veterinary Research Communications - January 23, 2024 Category: Veterinary Research Source Type: research
Detection of hepatitis E virus genotype 3 in an Algerian mouse (Mus spretus) in Portugal
AbstractVirus monitoring in small mammals is central to the design of epidemiological control strategies for rodent-borne zoonotic viruses. Synanthropic small mammals are versatile and may be potential carriers of several microbial agents. In the present work, a total of 330 fecal samples of small mammals were collected at two sites in the North of Portugal and screened for zoonotic hepatitis E virus (HEV, speciesPaslahepevirus balayani). Synanthropic small mammal samples (n = 40) were collected in a city park of Porto and belonged to the species Algerian mouse (Mus spretus) (n = 26) and to the greater white-toothe...
Source: Veterinary Research Communications - January 20, 2024 Category: Veterinary Research Source Type: research
Effects of organic and inorganic selenium supplementation on frozen-thawed ram semen at two cooling periods
AbstractThe aim of this study was to evaluate the effects of different selenium compounds on the sperm quality of cryopreserved ram semen. Ejaculates from four rams, collected using an artificial vagina heated to 38 °C, were individually evaluated. The approved ejaculates were pooled and diluted (1:1 v:v) in Tris-egg yolk extender (20%, v/v) and separated into two control groups, one cooled for 2 h and the other for 4 h. The pooled ejaculates at the two cooling periods were supplemented with two doses (0.5 and 1 μg/mL) of organic selenium (ORG), and inorganic selenium (SeNa), each. The samples were packed in 0.25 m...
Source: Veterinary Research Communications - January 20, 2024 Category: Veterinary Research Source Type: research
Salmonella serovars trend in poultry Malaysia from 2011 to 2020
This study could help the authorities to improvise policies for better disease control programs through the establishment of diagnostic tools for rapidSalmonella screening in poultry. (Source: Veterinary Research Communications)
Source: Veterinary Research Communications - January 19, 2024 Category: Veterinary Research Source Type: research
Detection of Coxiella burnetii in the mammary gland of a dairy goat
AbstractThe zoonotic bacteriumCoxiella (C.) burnetii can be excreted by infected goats through birth products and milk. The detection ofC. burnetii DNA in the mammary gland tissue of infected dairy goats and intermittent milk shedders has been reported, but confirmation ofC. burnetii bacteria in the udder remained pending. The pathogen caused abortions in a 152-head dairy goat herd, resulting in the vaccination againstC. burnetii of the entire herd with annual boosters. To monitor theC. burnetii shedding at herd level, monthly bulk tank milk (BTM) samples were analyzed using PCR (IS1111). Despite vaccination,C. burnetii DN...
Source: Veterinary Research Communications - January 18, 2024 Category: Veterinary Research Source Type: research
Isolation of lactic acid bacteria from the reproductive tract of mares as potentially beneficial strains to prevent equine endometritis
AbstractEndometritis, the inflammation of the endometrium, is the leading cause of subfertility in mares, and therefore responsible for major economic losses in the horse industry worldwide. It is generally treated with uterine lavages combined with ecbolic agents and local or systemic antibiotics. However, since antibiotic overuse has been associated with antimicrobial resistance in mares with persistent endometritis, new prevention and treatment alternatives are needed. One such alternative could be the use of probiotic lactic acid bacteria (LAB) isolated from the host. Thanks to their species specificity, resident micro...
Source: Veterinary Research Communications - January 18, 2024 Category: Veterinary Research Source Type: research
A long-term study on free-ranging jaguar-tick interactions, featuring a novel report of Amblyomma incisum adult infestation
AbstractOver nearly 12 years, we collected ticks from free-ranging jaguars (Panthera onca) and performed statistical analyses to comprehend the vector-host relationship throughout the seasons. We evaluated the presence and number of ticks, as well as their association with weight, age, and gender of captured jaguars in the Pantanal and Amazon biomes. Out of 100 captured jaguars (comprising 72 initial captures and 28 recaptures, with 41 females and 31 males), 77 were found to be infested by different tick species. We gathered a total of 1,002 ticks, categorized by the following species in descending order of abundance:Ambly...
Source: Veterinary Research Communications - January 17, 2024 Category: Veterinary Research Source Type: research
Molecular identification of Borrelia and SFG Rickettsia spp. in hard ticks parasitizing domestic and wild animals in southeastern Spain
This study increases the knowledge about the presence ofBorrelia spp. DNA and SFGRickettsia spp. DNA in different hard tick species from this geographical area. (Source: Veterinary Research Communications)
Source: Veterinary Research Communications - January 17, 2024 Category: Veterinary Research Source Type: research
High carbohydrate is preferable to high lipid parenteral nutrition in healthy dogs undergoing prolonged sedation
In conclusion, both PN protocols can be used in healthy animals undergoing prolonged sedation protocols. However, high lipid PN had higher VO2 and DEE, and resulted in higher triglycerides concentrations and lower glycaemia indexes than carbohydrate, making high carbohydrate PN preferable to high lipid PN. Therefore, for use in critically ill patients, the data obtained in this study should be extrapolated, taking into consideration the specificity of each case. (Source: Veterinary Research Communications)
Source: Veterinary Research Communications - January 17, 2024 Category: Veterinary Research Source Type: research
The impact of induced pluripotent stem cells in animal conservation
AbstractIt is widely acknowledged that we are currently facing a critical tipping point with regards to global extinction, with human activities driving us perilously close to the brink of a devastating sixth mass extinction. As a promising option for safeguarding endangered species, induced pluripotent stem cells (iPSCs) hold great potential to aid in the preservation of threatened animal populations. For endangered species, such as the northern white rhinoceros (Ceratotherium simum cottoni), supply of embryos is often limited. After the death of the last male in 2019, only two females remained in the world. IPSC technolo...
Source: Veterinary Research Communications - January 16, 2024 Category: Veterinary Research Source Type: research
Natural infection of free-ranging capybaras (Hydrochoerus hydrochaeris) with Anaplasmataecea and Rickettsiaceae bacteria in the Iber á wetlands ecoregion, Argentina
AbstractThe current work assessed the infection withEhrlichia andAnaplasma species, and exposure toRickettsia spp. in free-ranging capybaras in the Iber á wetlands ecoregion in Argentina. By indirect immunofluorescence assay, 37 out of 51 (73%) capybara sera were seropositive toRickettsia spp., with 23.5% and 4% samples considered homologous toRickettsia parkeri andRickettsia bellii, respectively (or very closely related serotypes). Anaplasmataceae DNA was found to be highly prevalent in capybaras, with 33 out of 62 samples positive forAnaplasma sp. withCt values of 28.64 ± 0.35 (average ± standard error), and ...
Source: Veterinary Research Communications - January 16, 2024 Category: Veterinary Research Source Type: research
Molecular screening of tick-borne microorganisms in ticks from rural areas of Panama, with the first record of Ehrlichia minasensis in Rhipicephalus microplus from Central America
AbstractDuring a molecular screening of rickettsial, borrelial and protozoal agents in ticks from rural areas of Panama, we detected DNA ofRickettsia amblyommatis in fourAmblyomma mixtum collected from horses in a locality in Los Santos province.Ehrlichia minasensis andAnaplasma marginale were identified from three and twoRhipicephalus microplus, respectively, collected on cows from Chiriqui province. No DNA from other tick-borne agents was found. These results increase the information ofR. amblyommatis andA. marginale in Panama, and also mark the first record ofE. minasensis in Central America. (Source: Veterinary Research Communications)
Source: Veterinary Research Communications - January 15, 2024 Category: Veterinary Research Source Type: research
