A comparison of dermal toxicity models; assessing suitability for safe(r)-by-design decision-making and for screening nanomaterial hazards
In this study, we compare in vitro and ex vivo cell models that represent dermal exposures (including HaCaT cells, primary keratinocytes, and reconstructed human epidermis (RhE)), and when possible consider these in the context of regulatory accepted OECD TG for in vitro dermal irritation. Various benchmark nanomaterials were used to assess markers of cell stress in each cell model. In addition, we evaluated different dosing strategies that have been used when applying the OECD TG for dermal irritation in assessment of nanomaterials, and how inconsistencies in the approach used can have considerable impact of the conclusio...
Source: Toxicology in Vitro - February 16, 2024 Category: Toxicology Authors: Polly McLean Jessica Marshall Amaia Garc ía David Beal Alberto Katsumiti Marie Carri ère Matthew Boyles Source Type: research
A comparison of dermal toxicity models; assessing suitability for safe(r)-by-design decision-making and for screening nanomaterial hazards
In this study, we compare in vitro and ex vivo cell models that represent dermal exposures (including HaCaT cells, primary keratinocytes, and reconstructed human epidermis (RhE)), and when possible consider these in the context of regulatory accepted OECD TG for in vitro dermal irritation. Various benchmark nanomaterials were used to assess markers of cell stress in each cell model. In addition, we evaluated different dosing strategies that have been used when applying the OECD TG for dermal irritation in assessment of nanomaterials, and how inconsistencies in the approach used can have considerable impact of the conclusio...
Source: Toxicology in Vitro - February 16, 2024 Category: Toxicology Authors: Polly McLean Jessica Marshall Amaia Garc ía David Beal Alberto Katsumiti Marie Carri ère Matthew Boyles Source Type: research
A comparison of dermal toxicity models; assessing suitability for safe(r)-by-design decision-making and for screening nanomaterial hazards
In this study, we compare in vitro and ex vivo cell models that represent dermal exposures (including HaCaT cells, primary keratinocytes, and reconstructed human epidermis (RhE)), and when possible consider these in the context of regulatory accepted OECD TG for in vitro dermal irritation. Various benchmark nanomaterials were used to assess markers of cell stress in each cell model. In addition, we evaluated different dosing strategies that have been used when applying the OECD TG for dermal irritation in assessment of nanomaterials, and how inconsistencies in the approach used can have considerable impact of the conclusio...
Source: Toxicology in Vitro - February 16, 2024 Category: Toxicology Authors: Polly McLean Jessica Marshall Amaia Garc ía David Beal Alberto Katsumiti Marie Carri ère Matthew Boyles Source Type: research
Gill epithelial cell line ASG-10 from Atlantic salmon as a new research tool for solving water quality challenges in aquaculture
In conclusion, the ASG-10 cell line is a promising in vitro model to study water quality challenges and whole water samples.PMID:38355023 | DOI:10.1016/j.tiv.2024.105790 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - February 14, 2024 Category: Toxicology Authors: Anita Solhaug P ål A Olsvik Prabhugouda Siriyappagouder Randi Faller Torstein Kristensen Source Type: research
Development of natural cosmetic emulsion using the by-product of Lecythis pisonis seed
Toxicol In Vitro. 2024 Feb 12:105791. doi: 10.1016/j.tiv.2024.105791. Online ahead of print.ABSTRACTNatural products and their biological activities are currently a subject of great interest to the industrial and scientific sector, due to society's awareness of the proper use of biodiversity and economic and sustainability. To promote the sustainable use of biomass the extract of the by-product of the shell seed of Lecythis pisonis was applied to develop a natural cosmetic emulsion. To ensure safety for its topical use the cytotoxic activity of its crude extract was evaluated by the colorimetric method of 3- bromide (4,5-d...
Source: Toxicology in Vitro - February 14, 2024 Category: Toxicology Authors: Danielle Braga Portes Lucas Wetler Abeldt Cristiane Dos Santos Giubert Denise Coutinho Endringer Elis ângela Flávia Pimentel Source Type: research
Gill epithelial cell line ASG-10 from Atlantic salmon as a new research tool for solving water quality challenges in aquaculture
In conclusion, the ASG-10 cell line is a promising in vitro model to study water quality challenges and whole water samples.PMID:38355023 | DOI:10.1016/j.tiv.2024.105790 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - February 14, 2024 Category: Toxicology Authors: Anita Solhaug P ål A Olsvik Prabhugouda Siriyappagouder Randi Faller Torstein Kristensen Source Type: research
Development of natural cosmetic emulsion using the by-product of Lecythis pisonis seed
Toxicol In Vitro. 2024 Feb 12:105791. doi: 10.1016/j.tiv.2024.105791. Online ahead of print.ABSTRACTNatural products and their biological activities are currently a subject of great interest to the industrial and scientific sector, due to society's awareness of the proper use of biodiversity and economic and sustainability. To promote the sustainable use of biomass the extract of the by-product of the shell seed of Lecythis pisonis was applied to develop a natural cosmetic emulsion. To ensure safety for its topical use the cytotoxic activity of its crude extract was evaluated by the colorimetric method of 3- bromide (4,5-d...
Source: Toxicology in Vitro - February 14, 2024 Category: Toxicology Authors: Danielle Braga Portes Lucas Wetler Abeldt Cristiane Dos Santos Giubert Denise Coutinho Endringer Elis ângela Flávia Pimentel Source Type: research
Interaction of mycotoxins zearalenone, α-zearalenol, and β-zearalenol with cytochrome P450 (CYP1A2, 2C9, 2C19, 2D6, and 3A4) enzymes and organic anion transporting polypeptides (OATP1A2, OATP1B1, OATP1B3, and OATP2B1)
Toxicol In Vitro. 2024 Feb 8;96:105789. doi: 10.1016/j.tiv.2024.105789. Online ahead of print.ABSTRACTZearalenone (ZEN) is a mycoestrogen produced by Fusarium fungi. ZEN is a frequent contaminant in cereal-based products, representing significant health threat. The major reduced metabolites of ZEN are α-zearalenol (α-ZEL) and β-zearalenol (β-ZEL). Since the toxicokinetic interactions of ZEN/ZELs with cytochrome P450 enzymes (CYPs) and organic anion transporting polypeptides (OATPs) have been barely characterized, we examined these interactions applying in vitro models. ZEN and ZELs were relatively strong inhibitors of ...
Source: Toxicology in Vitro - February 10, 2024 Category: Toxicology Authors: Hana Kaci Ágnes Dombi Patrik G ömbös Andr ás Szabó Éva Bakos Csilla Özvegy-Laczka Mikl ós Poór Source Type: research
Purinergic ligands induce extracellular acidification and increased ATP turnover in HepG2 cells
Toxicol In Vitro. 2024 Feb 4;96:105788. doi: 10.1016/j.tiv.2024.105788. Online ahead of print.ABSTRACTNucleosides and nucleotides at μM concentrations stimulated a 300% increase in acid secretion in HepG2 cells, which was quantitatively accounted for as increased export of lactate generated by glycogenolysis. Agonist selectivity encompassed nucleosides and nucleotides for all 5 natural nucleobases and, along with antagonist profiles, was inconsistent with a role for purinergic receptors in mediating this activity. Agonist catabolism did not contribute significantly to either low selectivity or lactate production. Lactate ...
Source: Toxicology in Vitro - February 6, 2024 Category: Toxicology Authors: Haotong Chen Yong Han Abby Hearne Anna Monarchino Jeffrey S Wiseman Source Type: research
Anti-migratory and cytotoxic effect of indole derivative in C6 glioma cells
Toxicol In Vitro. 2024 Feb 1;96:105786. doi: 10.1016/j.tiv.2024.105786. Online ahead of print.ABSTRACTGliomas are among the most common primary malignant brain tumors. Despite advances in cancer treatment, survival is very low, so the discovery of new therapeutic agents is essential. In this context, indole is an important source for the development of new bioactive molecules. A pharmacological screening of ten indole derivatives was carried out to evaluate the cytotoxic capacity against three tumor cell lines. After pharmacological screening, three compounds were selected, based on their high capacity to reduce cell proli...
Source: Toxicology in Vitro - February 1, 2024 Category: Toxicology Authors: Ana Karolina de Souza Andrade Mariana Nobre Farias de Franca Jileno Ferreira Santos Nath ália Araújo Macêdo Waldecy de Lucca Junior Ricardo Scher Socrates Cabral de Holanda Cavalcanti Cristiane Bani Corr êa Source Type: research
Arsenic trioxide inhibits the response of primary human B cells to influenza virus A in vitro
Toxicol In Vitro. 2024 Jan 24;96:105783. doi: 10.1016/j.tiv.2024.105783. Online ahead of print.ABSTRACTArsenic compounds are common environmental toxicants worldwide and particularly enriched in the Northeast and the Southwestern United States, the Alps, and Bangladesh. Exposure to arsenic is linked with various detrimental health outcomes, including cancer, cognitive decline, and kidney damage. Our group has previously shown that arsenic trioxide alters T cell cytokine production. In the current study, we demonstrate that exposure to arsenic compounds alters B cell function in an in vitro influenza model. Human peripheral...
Source: Toxicology in Vitro - January 26, 2024 Category: Toxicology Authors: Luca M Kaiser Robert A Freeborn Allison P Boss Yining Jin Cheryl E Rockwell Source Type: research
Biochanin A - A G6PD inhibitor: In silico and in vitro studies in non-small cell lung cancer cells (A549)
In conclusion, our findings highlight the potential of BA as a therapeutic agent in cancer treatment, specifically by targeting G6PD and related pathways. BA's varied effects, which range from anti-inflammatory capabilities to metastasis reduction, make it an appealing option for future investigation in the development of new cancer therapeutics.PMID:38266663 | DOI:10.1016/j.tiv.2024.105785 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - January 24, 2024 Category: Toxicology Authors: Anjali B Thakkar Ramalingam B Subramanian Sampark S Thakkar Vasudev R Thakkar Parth Thakor Source Type: research
Ursodeoxycholic acid and 18 β-glycyrrhetinic acid alleviate ethinylestradiol-induced cholestasis via downregulating RORγt and CXCR3 signaling pathway in iNKT cells
In conclusion, our observations provide new therapeutic targets of UDCA and 18β-GA, and 18β-GA as an alternative treatment for EE-induced cholestasis.PMID:38244730 | DOI:10.1016/j.tiv.2024.105782 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - January 20, 2024 Category: Toxicology Authors: Xinyu Li Xiaojing Liang Xiaoxia Gu Mengzhi Zou Weiping Cao Chunhui Liu Xinzhi Wang Source Type: research
PBA alleviates cadmium-induced mouse spermatogonia apoptosis by suppressing endoplasmic reticulum stress
CONCLUSION: PBA alleviated Cd-induced ER stress and spermatogonia apoptosis, and may have the therapeutic role in Cd-induced male reproductive toxicity.PMID:38242296 | DOI:10.1016/j.tiv.2024.105784 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - January 19, 2024 Category: Toxicology Authors: Rong Wang Mengyuan Li Zhen Wu Wenjing Gong Mingming Zhang Yehao Liu Yuyou Yao Yanli Ji Source Type: research
ESTIV 2022: Key enabling non-animal technologies for research, education and testing
Toxicol In Vitro. 2024 Jan 12:105779. doi: 10.1016/j.tiv.2024.105779. Online ahead of print.NO ABSTRACTPMID:38219805 | DOI:10.1016/j.tiv.2024.105779 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - January 14, 2024 Category: Toxicology Authors: Helena Kand árová Clive Roper Marketa Dvo řáková Source Type: research
