In vitro screening of per- and polyfluorinated substances (PFAS) for interference with seven thyroid hormone system targets across nine assays
The objective of this study was to evaluate the activity of 136 perfluorinated chemicals at seven key molecular initiating events (MIE) within the thyroid axis using nine in vitro assays. The potential MIE targets investigated are Human Iodothyronine Deiodinase 1 (hDIO1), Human Iodothyronine Deiodinase 2 (hDIO2), Human Iodothyronine Deiodinase 3 (hDIO3), Xenopus Iodothyronine Deiodinase (xDIO3); Human Iodotyrosine Deiodinase (hIYD), Xenopus Iodotyrosine Deiodinase (xIYD), Human Thyroid Peroxidase (hTPO); and the serum binding proteins Human Transthyretin (hTTR) and Human Thyroxine Binding Globulin (hTBG). Of the 136 PFAS c...
Source: Toxicology in Vitro - December 10, 2023 Category: Toxicology Authors: Sigmund J Degitz Jennifer H Olker None Jeffrey S Denny Philip P Degoey Phillip C Hartig Mary C Cardon Stephanie A Eytcheson Jonathan T Haselman Sally A Mayasich Michael W Hornung Source Type: research
Comparative studies on the cytotoxic effects induced by iron oxide nanoparticles in cancerous and noncancerous human lung cells subjected to an alternating magnetic field
Toxicol In Vitro. 2023 Dec 7:105760. doi: 10.1016/j.tiv.2023.105760. Online ahead of print.ABSTRACTThe cytotoxic effects of water-based ferrofluids composed of iron oxide nanoparticles, including magnetite (Fe3O4) and maghemite (γ-Fe2O3), ranging from 15 to 100 nm, were examined on various lung cancer cells including adenocarcinomic human alveolar basal epithelial cells (A549), nonsmall lung squamous cell carcinoma (H1703), small cell lung cancer cells (DMS 114), and normal bronchial epithelial cells (BEAS-2B). The cytotoxic effect was evaluated both with and without exposure to an alternating magnetic field (AMF). The st...
Source: Toxicology in Vitro - December 9, 2023 Category: Toxicology Authors: Monika Ruzycka-Ayoush Kamil Sobczak Ireneusz P Grudzinski Source Type: research
The mechanism of anticancer effects of some pyrrolopyrimidine derivatives on HT-29 human colon cancer cells
Toxicol In Vitro. 2023 Dec 5:105757. doi: 10.1016/j.tiv.2023.105757. Online ahead of print.ABSTRACTIn the present work, mechanism of anticancer activity of some pyrrolopyrimidine derivatives was evaluated. Compounds 5 and 8 exhibiting significant antiproliferative activity against HT-29 cells with IC50 values of 4.17 μM and 2.96, arrested the cells at the G2/M phase and significantly induced apoptosis. The apoptotic potential of the compounds has been verified via ELISA assay, which resulted in increased BAX, PUMA, BIM, and cleaved caspase 3 expression and decreased BCL-XL and MCL-1 protein levels in HT-29 cells. Moreover...
Source: Toxicology in Vitro - December 7, 2023 Category: Toxicology Authors: Mustafa Ergul Zuhal Kilic-Kurt Yeliz Aka Ozgur Kutuk Zeynep Deniz Şahin İnan Source Type: research
Betulinic acid inhibits the proliferation of human laryngeal carcinoma cells through reactive oxygen species-mediate mitochondrial apoptotic pathway
In this study, we examined how BA works to inhibit human laryngeal cancer growth. We discovered that BA minimally exhibited cytotoxicity in normal cells (human normal cell line GES-1), while remarkably inhibiting viability of AMC-HN-8, TU212, HEp-2 and M4e cells in a concentration-dependent manner. In AMC-HN-8 cancer cells, BA induced apoptosis, activated caspase-3/9/PARP, significantly reduced mitochondrial membrane potential (MMP), increased the expression of cytochrome C in the cytoplasm, transported Bax to the mitochondria, increased the production of reactive oxygen species (ROS), and the ROS scavenger N-acetylcystein...
Source: Toxicology in Vitro - December 7, 2023 Category: Toxicology Authors: Huamin Zhang Mengru Zhou Chongtao Ye Jiahao Qin Xuan Lu Chunyan Wang Xude Wang Xuejun Jin Source Type: research
Nilotinib exhibits less toxicity than imatinib and influences the immune state by modulating iNOS, p-p38 and p-JNK in LPS/IFN gamma-activated macrophages
In conclusion, our results reveal that, in addition to its anticancer activity, nilotinib can exhibit immune modulatory effects on macrophages through its effects on iNOS, IL-6, p-p38 and p-JNK.PMID:38061604 | DOI:10.1016/j.tiv.2023.105754 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - December 7, 2023 Category: Toxicology Authors: Arzu Zeynep Karabay Tulin Ozkan Asli Koc Yalda Hekmatshoar A Selen Gurkan-Alp Asuman Sunguroglu Source Type: research
Changes in attachment and metabolic activity of rat neonatal cardiomyocytes and nonmyocytes caused by Macrovipera lebetina obtusa venom
We examined the influence of MLO venom (crude and with inhibited metalloproteinases and phospholipase A2) on attachment and metabolic activity of rat neonatal cardiomyocytes (CM) and nonmyocytes (nCM), assessed at 1 and 24 h. After exposing both CM and nCM to varying concentrations of MLO venom, we observed immediate cytotoxic effects at a concentration of 100 μg/ml, causing detachment from the culture substrate. At lower MLO venom concentrations both cell types detached in a dose-dependent manner. Inhibition of MLO venom metalloproteinases significantly improved CM and nCM attachment after 1-hour exposure. At 24-hour exp...
Source: Toxicology in Vitro - December 7, 2023 Category: Toxicology Authors: Hovhannes Arestakesyan Narine LeFevre Nikki Posnack Arni Sarian Vahan Grigorian Naira Ayvazyan Armen Voskanyan Narine Sarvazyan Zaruhi Karabekian Source Type: research
The mechanism of anticancer effects of some pyrrolopyrimidine derivatives on HT-29 human colon cancer cells
Toxicol In Vitro. 2023 Dec 5:105757. doi: 10.1016/j.tiv.2023.105757. Online ahead of print.ABSTRACTIn the present work, mechanism of anticancer activity of some pyrrolopyrimidine derivatives was evaluated. Compounds 5 and 8 exhibiting significant antiproliferative activity against HT-29 cells with IC50 values of 4.17 μM and 2.96, arrested the cells at the G2/M phase and significantly induced apoptosis. The apoptotic potential of the compounds has been verified via ELISA assay, which resulted in increased BAX, PUMA, BIM, and cleaved caspase 3 expression and decreased BCL-XL and MCL-1 protein levels in HT-29 cells. Moreover...
Source: Toxicology in Vitro - December 7, 2023 Category: Toxicology Authors: Mustafa Ergul Zuhal Kilic-Kurt Yeliz Aka Ozgur Kutuk Zeynep Deniz Şahin İnan Source Type: research
Betulinic acid inhibits the proliferation of human laryngeal carcinoma cells through reactive oxygen species-mediate mitochondrial apoptotic pathway
In this study, we examined how BA works to inhibit human laryngeal cancer growth. We discovered that BA minimally exhibited cytotoxicity in normal cells (human normal cell line GES-1), while remarkably inhibiting viability of AMC-HN-8, TU212, HEp-2 and M4e cells in a concentration-dependent manner. In AMC-HN-8 cancer cells, BA induced apoptosis, activated caspase-3/9/PARP, significantly reduced mitochondrial membrane potential (MMP), increased the expression of cytochrome C in the cytoplasm, transported Bax to the mitochondria, increased the production of reactive oxygen species (ROS), and the ROS scavenger N-acetylcystein...
Source: Toxicology in Vitro - December 7, 2023 Category: Toxicology Authors: Huamin Zhang Mengru Zhou Chongtao Ye Jiahao Qin Xuan Lu Chunyan Wang Xude Wang Xuejun Jin Source Type: research
Nilotinib exhibits less toxicity than imatinib and influences the immune state by modulating iNOS, p-p38 and p-JNK in LPS/IFN gamma-activated macrophages
In conclusion, our results reveal that, in addition to its anticancer activity, nilotinib can exhibit immune modulatory effects on macrophages through its effects on iNOS, IL-6, p-p38 and p-JNK.PMID:38061604 | DOI:10.1016/j.tiv.2023.105754 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - December 7, 2023 Category: Toxicology Authors: Arzu Zeynep Karabay Tulin Ozkan Asli Koc Yalda Hekmatshoar A Selen Gurkan-Alp Asuman Sunguroglu Source Type: research
Changes in attachment and metabolic activity of rat neonatal cardiomyocytes and nonmyocytes caused by Macrovipera lebetina obtusa venom
We examined the influence of MLO venom (crude and with inhibited metalloproteinases and phospholipase A2) on attachment and metabolic activity of rat neonatal cardiomyocytes (CM) and nonmyocytes (nCM), assessed at 1 and 24 h. After exposing both CM and nCM to varying concentrations of MLO venom, we observed immediate cytotoxic effects at a concentration of 100 μg/ml, causing detachment from the culture substrate. At lower MLO venom concentrations both cell types detached in a dose-dependent manner. Inhibition of MLO venom metalloproteinases significantly improved CM and nCM attachment after 1-hour exposure. At 24-hour exp...
Source: Toxicology in Vitro - December 7, 2023 Category: Toxicology Authors: Hovhannes Arestakesyan Narine LeFevre Nikki Posnack Arni Sarian Vahan Grigorian Naira Ayvazyan Armen Voskanyan Narine Sarvazyan Zaruhi Karabekian Source Type: research
The mechanism of anticancer effects of some pyrrolopyrimidine derivatives on HT-29 human colon cancer cells
Toxicol In Vitro. 2023 Dec 5:105757. doi: 10.1016/j.tiv.2023.105757. Online ahead of print.ABSTRACTIn the present work, mechanism of anticancer activity of some pyrrolopyrimidine derivatives was evaluated. Compounds 5 and 8 exhibiting significant antiproliferative activity against HT-29 cells with IC50 values of 4.17 μM and 2.96, arrested the cells at the G2/M phase and significantly induced apoptosis. The apoptotic potential of the compounds has been verified via ELISA assay, which resulted in increased BAX, PUMA, BIM, and cleaved caspase 3 expression and decreased BCL-XL and MCL-1 protein levels in HT-29 cells. Moreover...
Source: Toxicology in Vitro - December 7, 2023 Category: Toxicology Authors: Mustafa Ergul Zuhal Kilic-Kurt Yeliz Aka Ozgur Kutuk Zeynep Deniz Şahin İnan Source Type: research
Betulinic acid inhibits the proliferation of human laryngeal carcinoma cells through reactive oxygen species-mediate mitochondrial apoptotic pathway
In this study, we examined how BA works to inhibit human laryngeal cancer growth. We discovered that BA minimally exhibited cytotoxicity in normal cells (human normal cell line GES-1), while remarkably inhibiting viability of AMC-HN-8, TU212, HEp-2 and M4e cells in a concentration-dependent manner. In AMC-HN-8 cancer cells, BA induced apoptosis, activated caspase-3/9/PARP, significantly reduced mitochondrial membrane potential (MMP), increased the expression of cytochrome C in the cytoplasm, transported Bax to the mitochondria, increased the production of reactive oxygen species (ROS), and the ROS scavenger N-acetylcystein...
Source: Toxicology in Vitro - December 7, 2023 Category: Toxicology Authors: Huamin Zhang Mengru Zhou Chongtao Ye Jiahao Qin Xuan Lu Chunyan Wang Xude Wang Xuejun Jin Source Type: research
Nilotinib exhibits less toxicity than imatinib and influences the immune state by modulating iNOS, p-p38 and p-JNK in LPS/IFN gamma-activated macrophages
In conclusion, our results reveal that, in addition to its anticancer activity, nilotinib can exhibit immune modulatory effects on macrophages through its effects on iNOS, IL-6, p-p38 and p-JNK.PMID:38061604 | DOI:10.1016/j.tiv.2023.105754 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - December 7, 2023 Category: Toxicology Authors: Arzu Zeynep Karabay Tulin Ozkan Asli Koc Yalda Hekmatshoar A Selen Gurkan-Alp Asuman Sunguroglu Source Type: research
Changes in attachment and metabolic activity of rat neonatal cardiomyocytes and nonmyocytes caused by Macrovipera lebetina obtusa venom
We examined the influence of MLO venom (crude and with inhibited metalloproteinases and phospholipase A2) on attachment and metabolic activity of rat neonatal cardiomyocytes (CM) and nonmyocytes (nCM), assessed at 1 and 24 h. After exposing both CM and nCM to varying concentrations of MLO venom, we observed immediate cytotoxic effects at a concentration of 100 μg/ml, causing detachment from the culture substrate. At lower MLO venom concentrations both cell types detached in a dose-dependent manner. Inhibition of MLO venom metalloproteinases significantly improved CM and nCM attachment after 1-hour exposure. At 24-hour exp...
Source: Toxicology in Vitro - December 7, 2023 Category: Toxicology Authors: Hovhannes Arestakesyan Narine LeFevre Nikki Posnack Arni Sarian Vahan Grigorian Naira Ayvazyan Armen Voskanyan Narine Sarvazyan Zaruhi Karabekian Source Type: research
A multiparametric and orthogonal approach indicates low toxicity for zein nanoparticles in a repellent formulation
This study evaluates the cytotoxicity of a repellent formulation containing zein nanoparticles (NP) loading geraniol (Ger) and icaridin (Ica) in three cell lines: NIH/3T3, HaCaT, and SIRC. To address formulation hazards, IC50 values were determined by MTT and Calcein-AM assays. In both NIH/3T3 and HaCaT, the IC50 values for NP + Ger + Ica formulation were around 0.2%. For risk assessment, cell viability was also determined after a single exposure and repeated exposure to the formulation. No evidence of cytotoxicity was observed for NP + Ger + Ica formulation-treated cells. The risk assessment for eye damage revealed cytoto...
Source: Toxicology in Vitro - December 3, 2023 Category: Toxicology Authors: Tuanny Leite Almeida Aline Francisca Moreira Jhones Luiz de Oliveira Carolina Barbara Rogerio Samara Flamini Kiihl Leonardo Fernandes Fraceto Marcelo Bispo de Jesus Source Type: research
