Utilizing primary human airway mucociliary tissue cultures to model ramifications of chronic E-cigarette usage
Toxicol In Vitro. 2023 Oct 24:105725. doi: 10.1016/j.tiv.2023.105725. Online ahead of print.ABSTRACTElectronic cigarettes are battery powered devices that use a vape-liquid to produce a vapor that is inhaled. A consequence of the rise in e-cigarette usage was the 2019 emergence of a vaping-induced respiratory disease denoted as 'e-cigarette or vaping use-associated lung injury' (EVALI). One of the suspected causes of EVALI is Vitamin E Acetate (VEA), which was found to be a diluent in certain illicit vape-pens, whereas nicotine is commonly diluted in equal parts propylene glycol and vegetable glycerin (PG:VG). The prevalen...
Source: Toxicology in Vitro - October 26, 2023 Category: Toxicology Authors: Vincent J Manna Shannon Dwyer Vanessa Pizutelli Salvatore J Caradonna Source Type: research
The use of in silico molecular modelling to screen potential estrogen mimics as part of medicines and agrochemicals development and product license applications
Toxicol In Vitro. 2023 Oct 22:105721. doi: 10.1016/j.tiv.2023.105721. Online ahead of print.ABSTRACTEstrogen mimics are a diverse group of synthetic and naturally occurring compounds that can interact with estrogen receptors (ERs) in animals, including humans. These interactions rely on key structural features of 17b-estradiol (E2) and if these molecular features are in a similar spatial arrangement on other compounds, they are likely to elicit an agonist (i.e., they are E2 mimics) or antagonist response. The structural diversity of some compounds vis-à-vis analogies with E2 makes it difficult to reliably predict E2 mimic...
Source: Toxicology in Vitro - October 24, 2023 Category: Toxicology Authors: Rachel Z Bennie Ian C Shaw Source Type: research
The use of in silico molecular modelling to screen potential estrogen mimics as part of medicines and agrochemicals development and product license applications
Toxicol In Vitro. 2023 Oct 22:105721. doi: 10.1016/j.tiv.2023.105721. Online ahead of print.ABSTRACTEstrogen mimics are a diverse group of synthetic and naturally occurring compounds that can interact with estrogen receptors (ERs) in animals, including humans. These interactions rely on key structural features of 17b-estradiol (E2) and if these molecular features are in a similar spatial arrangement on other compounds, they are likely to elicit an agonist (i.e., they are E2 mimics) or antagonist response. The structural diversity of some compounds vis-à-vis analogies with E2 makes it difficult to reliably predict E2 mimic...
Source: Toxicology in Vitro - October 24, 2023 Category: Toxicology Authors: Rachel Z Bennie Ian C Shaw Source Type: research
The use of in silico molecular modelling to screen potential estrogen mimics as part of medicines and agrochemicals development and product license applications
Toxicol In Vitro. 2023 Oct 22:105721. doi: 10.1016/j.tiv.2023.105721. Online ahead of print.ABSTRACTEstrogen mimics are a diverse group of synthetic and naturally occurring compounds that can interact with estrogen receptors (ERs) in animals, including humans. These interactions rely on key structural features of 17b-estradiol (E2) and if these molecular features are in a similar spatial arrangement on other compounds, they are likely to elicit an agonist (i.e., they are E2 mimics) or antagonist response. The structural diversity of some compounds vis-à-vis analogies with E2 makes it difficult to reliably predict E2 mimic...
Source: Toxicology in Vitro - October 24, 2023 Category: Toxicology Authors: Rachel Z Bennie Ian C Shaw Source Type: research
How to use an in vitro approach to characterize the toxicity of airborne compounds
Toxicol In Vitro. 2023 Oct 21:105718. doi: 10.1016/j.tiv.2023.105718. Online ahead of print.ABSTRACTAs part of the development of new approach methodologies (NAMs), numerous in vitro methods are being developed to characterize the potential toxicity of inhalable xenobiotics (gases, volatile organic compounds, polycyclic aromatic hydrocarbons, particulate matter, nanoparticles). However, the materials and methods employed are extremely diverse, and no single method is currently in use. Method standardization and validation would raise trust in the results and enable them to be compared. This four-part review lists and compa...
Source: Toxicology in Vitro - October 23, 2023 Category: Toxicology Authors: Nour Jaber Sylvain Billet Source Type: research
m-Cresol,a pesticide intermediate, induces hepatotoxicity and behavioral abnormalities in zebrafish larvae through oxidative stress, apoptosis
In this study, zebrafish larvae were used to comprehensively study the hepatotoxicity of m-cresol and explore its molecular mechanism. After 72 hpf of fertilization, zebrafish larvae were exposed to 0.2 mM,0.4 mM, and 0.6 mM of m-Cresol. Varying degrees of liver injury and behavioral abnormalities were observed. The hepatotoxicity of zebrafish larvae may be induced by oxidative stress pathway and apoptosis of cell.PMID:37871866 | DOI:10.1016/j.tiv.2023.105723 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - October 23, 2023 Category: Toxicology Authors: Ying Wang Jie Wu Mengqi Wan Dou Yang Fasheng Liu Kehao Li Manxin Hu Yuanyuan Tang Huiqiang Lu Shouhua Zhang Yuanzhen Xiong Source Type: research
Puerarin alleviates hyperosmotic stress-induced oxidative stress, inflammation, apoptosis and barrier damage of human corneal epithelial cells by targeting SIRT1/NLRP3 signaling
In this study, we aimed to explore the effect and mechanism of hyperosmotic stress (Hyp)-induced human corneal epithelial cell line (HCE-2). The viability of HCE-2 cells induced by Hyp with or without puerarin treatment was assessed by a CCK-8 assay. Results indicated that puerarin treatment enhanced cell viability, reduced reactive oxygen species (ROS) content, increased CAT and SOD activities, and elevated the ratio of GSH/GSSG in HCE-2 cells exposed to Hyp. Besides, TNF-α, IL-1β and IL-6 contents were decreased by puerarin. Additionally, puerarin inhibited Hyp-induced apoptosis and barrier disruption of HCE-2 cells. M...
Source: Toxicology in Vitro - October 21, 2023 Category: Toxicology Authors: Yue Dong Yin-Yin Ding Wei-Ping Gao Source Type: research
Puerarin alleviates hyperosmotic stress-induced oxidative stress, inflammation, apoptosis and barrier damage of human corneal epithelial cells by targeting SIRT1/NLRP3 signaling
In this study, we aimed to explore the effect and mechanism of hyperosmotic stress (Hyp)-induced human corneal epithelial cell line (HCE-2). The viability of HCE-2 cells induced by Hyp with or without puerarin treatment was assessed by a CCK-8 assay. Results indicated that puerarin treatment enhanced cell viability, reduced reactive oxygen species (ROS) content, increased CAT and SOD activities, and elevated the ratio of GSH/GSSG in HCE-2 cells exposed to Hyp. Besides, TNF-α, IL-1β and IL-6 contents were decreased by puerarin. Additionally, puerarin inhibited Hyp-induced apoptosis and barrier disruption of HCE-2 cells. M...
Source: Toxicology in Vitro - October 21, 2023 Category: Toxicology Authors: Yue Dong Yin-Yin Ding Wei-Ping Gao Source Type: research
Puerarin alleviates hyperosmotic stress-induced oxidative stress, inflammation, apoptosis and barrier damage of human corneal epithelial cells by targeting SIRT1/NLRP3 signaling
In this study, we aimed to explore the effect and mechanism of hyperosmotic stress (Hyp)-induced human corneal epithelial cell line (HCE-2). The viability of HCE-2 cells induced by Hyp with or without puerarin treatment was assessed by a CCK-8 assay. Results indicated that puerarin treatment enhanced cell viability, reduced reactive oxygen species (ROS) content, increased CAT and SOD activities, and elevated the ratio of GSH/GSSG in HCE-2 cells exposed to Hyp. Besides, TNF-α, IL-1β and IL-6 contents were decreased by puerarin. Additionally, puerarin inhibited Hyp-induced apoptosis and barrier disruption of HCE-2 cells. M...
Source: Toxicology in Vitro - October 21, 2023 Category: Toxicology Authors: Yue Dong Yin-Yin Ding Wei-Ping Gao Source Type: research
Differential DNA damage response and cell fate in human lung cells after exposure to genotoxic compounds
Toxicol In Vitro. 2023 Oct 13;94:105710. doi: 10.1016/j.tiv.2023.105710. Online ahead of print.ABSTRACTDNA damage can impair normal cellular functions and result in various pathophysiological processes including cardiovascular diseases and cancer. We compared the genotoxic potential of diverse DNA damaging agents, and focused on their effects on the DNA damage response (DDR) and cell fate in human lung cells BEAS-2B. Polycyclic aromatic hydrocarbons [PAHs; benzo[a]pyrene (B[a]P), 1-nitropyrene (1-NP)] induced DNA strand breaks and oxidative damage to DNA; anticancer drugs doxorubicin (DOX) and 5-bromo-2'-deoxyuridine (BrdU...
Source: Toxicology in Vitro - October 14, 2023 Category: Toxicology Authors: H Libalova T Zavodna H Margaryan F Elzeinova A Milcova K Vrbova H Barosova T Cervena J Topinka P R össner Source Type: research
Differential DNA damage response and cell fate in human lung cells after exposure to genotoxic compounds
Toxicol In Vitro. 2023 Oct 13;94:105710. doi: 10.1016/j.tiv.2023.105710. Online ahead of print.ABSTRACTDNA damage can impair normal cellular functions and result in various pathophysiological processes including cardiovascular diseases and cancer. We compared the genotoxic potential of diverse DNA damaging agents, and focused on their effects on the DNA damage response (DDR) and cell fate in human lung cells BEAS-2B. Polycyclic aromatic hydrocarbons [PAHs; benzo[a]pyrene (B[a]P), 1-nitropyrene (1-NP)] induced DNA strand breaks and oxidative damage to DNA; anticancer drugs doxorubicin (DOX) and 5-bromo-2'-deoxyuridine (BrdU...
Source: Toxicology in Vitro - October 14, 2023 Category: Toxicology Authors: H Libalova T Zavodna H Margaryan F Elzeinova A Milcova K Vrbova H Barosova T Cervena J Topinka P R össner Source Type: research
Differential DNA damage response and cell fate in human lung cells after exposure to genotoxic compounds
Toxicol In Vitro. 2023 Oct 13;94:105710. doi: 10.1016/j.tiv.2023.105710. Online ahead of print.ABSTRACTDNA damage can impair normal cellular functions and result in various pathophysiological processes including cardiovascular diseases and cancer. We compared the genotoxic potential of diverse DNA damaging agents, and focused on their effects on the DNA damage response (DDR) and cell fate in human lung cells BEAS-2B. Polycyclic aromatic hydrocarbons [PAHs; benzo[a]pyrene (B[a]P), 1-nitropyrene (1-NP)] induced DNA strand breaks and oxidative damage to DNA; anticancer drugs doxorubicin (DOX) and 5-bromo-2'-deoxyuridine (BrdU...
Source: Toxicology in Vitro - October 14, 2023 Category: Toxicology Authors: H Libalova T Zavodna H Margaryan F Elzeinova A Milcova K Vrbova H Barosova T Cervena J Topinka P R össner Source Type: research
Differential DNA damage response and cell fate in human lung cells after exposure to genotoxic compounds
Toxicol In Vitro. 2023 Oct 12:105710. doi: 10.1016/j.tiv.2023.105710. Online ahead of print.ABSTRACTDNA damage can impair normal cellular functions and result in various pathophysiological processes including cardiovascular diseases and cancer. We compared the genotoxic potential of diverse DNA damaging agents, and focused on their effects on the DNA damage response (DDR) and cell fate in human lung cells BEAS-2B. Polycyclic aromatic hydrocarbons [PAHs; benzo[a]pyrene (B[a]P), 1-nitropyrene (1-NP)], induced DNA strand breaks and oxidative damage to DNA; anticancer drugs doxorubicin (DOX) and 5-bromo-2'-deoxyuridine (BrdU) ...
Source: Toxicology in Vitro - October 14, 2023 Category: Toxicology Authors: H Libalova T Zavodna H Margaryan F Elzeinova A Milcova K Vrbova H Barosova T Cervena J Topinka P R össner Source Type: research
Differential DNA damage response and cell fate in human lung cells after exposure to genotoxic compounds
Toxicol In Vitro. 2023 Oct 12:105710. doi: 10.1016/j.tiv.2023.105710. Online ahead of print.ABSTRACTDNA damage can impair normal cellular functions and result in various pathophysiological processes including cardiovascular diseases and cancer. We compared the genotoxic potential of diverse DNA damaging agents, and focused on their effects on the DNA damage response (DDR) and cell fate in human lung cells BEAS-2B. Polycyclic aromatic hydrocarbons [PAHs; benzo[a]pyrene (B[a]P), 1-nitropyrene (1-NP)], induced DNA strand breaks and oxidative damage to DNA; anticancer drugs doxorubicin (DOX) and 5-bromo-2'-deoxyuridine (BrdU) ...
Source: Toxicology in Vitro - October 14, 2023 Category: Toxicology Authors: H Libalova T Zavodna H Margaryan F Elzeinova A Milcova K Vrbova H Barosova T Cervena J Topinka P R össner Source Type: research
PSAT1 promotes autophagy to resist insufficient autophagy caused by cigarette smoke extract in human airway epithelial cells
This study discovered that CS significantly elevates PSAT1 expression in bronchial epithelial cells. Further studies using autophagy inhibitor, RNA interference, RT-qPCR, western blot, and CCK-8 assay in 16-HBE cells have confirmed that autophagy is temporarily initiated by cigarette smoke extract, but insufficient autophagy leads to cell death. PSAT1 induced by CSE promotes autophagy and resists insufficient autophagy caused by CSE through Akt/mTOR pathway in human bronchial epithelial cells, playing a protective role.PMID:37832835 | DOI:10.1016/j.tiv.2023.105711 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - October 13, 2023 Category: Toxicology Authors: Lixing Wang Furong Yan Yongbin Shi Yifei Liu Xiaoshan Su Yaping Zhang Source Type: research
