Evaluation of toxicity of heated tobacco products aerosol and cigarette smoke to BEAS-2B cells based on 3D biomimetic chip model
Toxicol In Vitro. 2023 Oct 6:105708. doi: 10.1016/j.tiv.2023.105708. Online ahead of print.ABSTRACTIt is still a controversial topic about evaluating whether heated tobacco products (HTP) really reduce harm, which involves the choice of an experimental model. Here, a three-dimensional (3D) biomimetic chip model was used to evaluate the toxicity of aerosols came from HTP and smoke produced by cigarettes (Cig). Based on cell-related experiments, we found that the toxicity of Cig smoke extract diluted four times was also much higher than that of undiluted HTP, showing higher oxidative stress response and cause mitochondrial d...
Source: Toxicology in Vitro - October 8, 2023 Category: Toxicology Authors: Hongjuan Wang Fengjun Lu Yushan Tian Sen Zhang Shulei Han Yaning Fu Jun Li Pengxia Feng Zhihao Shi Huan Chen Hongwei Hou Source Type: research
Effects of methylation of arginine residue 83 on the enzymatic activity of human arsenic (+3 oxidation state) methyltransferase
Toxicol In Vitro. 2023 Oct 5:105707. doi: 10.1016/j.tiv.2023.105707. Online ahead of print.ABSTRACTArsenic (+3 oxidation state) methyltransferase is an enzyme responsible for arsenic methylation, and it requires S-adenosyl-methionine (SAM) as a coenzyme. We here generated two mutants to clarify the role of the highly conserved 83rd arginine residue (Arg83) in Motif I, the SAM-binding domain, of human AS3MT. When the AS3MT activity was compared between the mutants and the wild type (WT) recombinant protein, little activity was detected in the glycine mutant (Arg83Gly) or lysine mutant (Arg83Lys). When we examined the abilit...
Source: Toxicology in Vitro - October 7, 2023 Category: Toxicology Authors: Daigo Sumi Yuuri Nakamura Hiroki Taguchi Yasuko Okamoto Hitomi Fujishiro Source Type: research
A battery of in silico models application for pesticides exerting reproductive health effects: Assessment of performance and prioritization of mechanistic studies
This study aimed at the assessment of the correlation between the predicted results of a battery of in silico tools and the reported observed adverse effects from in vivo reproductive toxicity studies. We used VirtualToxLab (VTL) software and the EndocrineDisruptome (ED) online tool to evaluate the binding affinities to nuclear receptors of 17 pesticides, 7 of which were classified as reprotoxic substances under Regulation (EC) No 1272/2008 on the classification, labelling and packaging of substances and mixtures (CLP). Then, we aligned the results of the in silico modelling with data from ToxCast assays and in vivo reprod...
Source: Toxicology in Vitro - October 6, 2023 Category: Toxicology Authors: Serhii Kolesnyk Mykola Prodanchuk Petro Zhminko Yana Kolianchuk Nataliia Bubalo Alex Odermatt Martin Smie ško Source Type: research
A battery of in silico models application for pesticides exerting reproductive health effects: Assessment of performance and prioritization of mechanistic studies
This study aimed at the assessment of the correlation between the predicted results of a battery of in silico tools and the reported observed adverse effects from in vivo reproductive toxicity studies. We used VirtualToxLab (VTL) software and the EndocrineDisruptome (ED) online tool to evaluate the binding affinities to nuclear receptors of 17 pesticides, 7 of which were classified as reprotoxic substances under Regulation (EC) No 1272/2008 on the classification, labelling and packaging of substances and mixtures (CLP). Then, we aligned the results of the in silico modelling with data from ToxCast assays and in vivo reprod...
Source: Toxicology in Vitro - October 6, 2023 Category: Toxicology Authors: Serhii Kolesnyk Mykola Prodanchuk Petro Zhminko Yana Kolianchuk Nataliia Bubalo Alex Odermatt Martin Smie ško Source Type: research
New insights into the cytotoxic effects of Thymus vulgaris essential oil on the human triple-negative breast cancer cell line MDA-MB-231
Toxicol In Vitro. 2023 Sep 27;93:105705. doi: 10.1016/j.tiv.2023.105705. Online ahead of print.ABSTRACTEssential oils (EOs) are natural products that have gained wide interest due to their biological activities and anticancer properties through various mechanisms. The present study aimed to test the cytotoxicity of Thymus vulgaris L. (thyme) EO of Italian origin, rich in thymol (49.6%) and p-cymene (18.8%), towards the triple-negative breast cancer cell line MDA-MB-231 and to investigate the biochemical mechanisms underlying its antitumor activity. Thyme EO reduced cancer cell viability in a dose-dependent manner after 24 ...
Source: Toxicology in Vitro - September 29, 2023 Category: Toxicology Authors: Serena Benedetti Maria Gemma Nasoni Francesca Luchetti Francesco Palma Source Type: research
New insights into the cytotoxic effects of Thymus vulgaris essential oil on the human triple-negative breast cancer cell line MDA-MB-231
Toxicol In Vitro. 2023 Sep 27;93:105705. doi: 10.1016/j.tiv.2023.105705. Online ahead of print.ABSTRACTEssential oils (EOs) are natural products that have gained wide interest due to their biological activities and anticancer properties through various mechanisms. The present study aimed to test the cytotoxicity of Thymus vulgaris L. (thyme) EO of Italian origin, rich in thymol (49.6%) and p-cymene (18.8%), towards the triple-negative breast cancer cell line MDA-MB-231 and to investigate the biochemical mechanisms underlying its antitumor activity. Thyme EO reduced cancer cell viability in a dose-dependent manner after 24 ...
Source: Toxicology in Vitro - September 29, 2023 Category: Toxicology Authors: Serena Benedetti Maria Gemma Nasoni Francesca Luchetti Francesco Palma Source Type: research
New insights into the cytotoxic effects of Thymus vulgaris essential oil on the human triple-negative breast cancer cell line MDA-MB-231
Toxicol In Vitro. 2023 Sep 27:105705. doi: 10.1016/j.tiv.2023.105705. Online ahead of print.ABSTRACTEssential oils (EOs) are natural products that have gained wide interest due to their biological activities and anticancer properties through various mechanisms. The present study aimed to test the cytotoxicity of Thymus vulgaris L. (thyme) EO of Italian origin, rich in thymol (49.6%) and p-cymene (18.8%), towards the triple-negative breast cancer cell line MDA-MB-231 and to investigate the biochemical mechanisms underlying its antitumor activity. Thyme EO reduced cancer cell viability in a dose-dependent manner after 24 h t...
Source: Toxicology in Vitro - September 29, 2023 Category: Toxicology Authors: Serena Benedetti Maria Gemma Nasoni Francesca Luchetti Francesco Palma Source Type: research
New insights into the cytotoxic effects of Thymus vulgaris essential oil on the human triple-negative breast cancer cell line MDA-MB-231
Toxicol In Vitro. 2023 Sep 27:105705. doi: 10.1016/j.tiv.2023.105705. Online ahead of print.ABSTRACTEssential oils (EOs) are natural products that have gained wide interest due to their biological activities and anticancer properties through various mechanisms. The present study aimed to test the cytotoxicity of Thymus vulgaris L. (thyme) EO of Italian origin, rich in thymol (49.6%) and p-cymene (18.8%), towards the triple-negative breast cancer cell line MDA-MB-231 and to investigate the biochemical mechanisms underlying its antitumor activity. Thyme EO reduced cancer cell viability in a dose-dependent manner after 24 h t...
Source: Toxicology in Vitro - September 29, 2023 Category: Toxicology Authors: Serena Benedetti Maria Gemma Nasoni Francesca Luchetti Francesco Palma Source Type: research
New insights into the cytotoxic effects of Thymus vulgaris essential oil on the human triple-negative breast cancer cell line MDA-MB-231
Toxicol In Vitro. 2023 Sep 27:105705. doi: 10.1016/j.tiv.2023.105705. Online ahead of print.ABSTRACTEssential oils (EOs) are natural products that have gained wide interest due to their biological activities and anticancer properties through various mechanisms. The present study aimed to test the cytotoxicity of Thymus vulgaris L. (thyme) EO of Italian origin, rich in thymol (49.6%) and p-cymene (18.8%), towards the triple-negative breast cancer cell line MDA-MB-231 and to investigate the biochemical mechanisms underlying its antitumor activity. Thyme EO reduced cancer cell viability in a dose-dependent manner after 24 h t...
Source: Toxicology in Vitro - September 29, 2023 Category: Toxicology Authors: Serena Benedetti Maria Gemma Nasoni Francesca Luchetti Francesco Palma Source Type: research
New insights into the cytotoxic effects of Thymus vulgaris essential oil on the human triple-negative breast cancer cell line MDA-MB-231
Toxicol In Vitro. 2023 Sep 27:105705. doi: 10.1016/j.tiv.2023.105705. Online ahead of print.ABSTRACTEssential oils (EOs) are natural products that have gained wide interest due to their biological activities and anticancer properties through various mechanisms. The present study aimed to test the cytotoxicity of Thymus vulgaris L. (thyme) EO of Italian origin, rich in thymol (49.6%) and p-cymene (18.8%), towards the triple-negative breast cancer cell line MDA-MB-231 and to investigate the biochemical mechanisms underlying its antitumor activity. Thyme EO reduced cancer cell viability in a dose-dependent manner after 24 h t...
Source: Toxicology in Vitro - September 29, 2023 Category: Toxicology Authors: Serena Benedetti Maria Gemma Nasoni Francesca Luchetti Francesco Palma Source Type: research
Osthole protects H9c2 cardiomyocytes against trastuzumab-induced damage by enhancing autophagy through the p38MAPK/mTOR signaling pathway
Toxicol In Vitro. 2023 Sep 26:105704. doi: 10.1016/j.tiv.2023.105704. Online ahead of print.ABSTRACTTrastuzumab (TRZ) is a novel targeted anti-tumor agent that significantly improve the survival of patients with human epidermal growth factor receptor (HER2) positive breast cancer. However, its clinical application is limited due to the side effects of cardiotoxicity. Osthol (OST), a coumarin derivative isolated from Cnidium monnieri (L.) Cusson, has previously demonstrated cardioprotective effects. The aim of this study was to observe the protective effect of OST on TRZ-induced cardiomyocytes damage and to explore its pote...
Source: Toxicology in Vitro - September 28, 2023 Category: Toxicology Authors: Huan Hou Yaping Yang Rong Chen Zhiping Guo Source Type: research
An examination of published datasets of skin permeability and partition coefficients
Toxicol In Vitro. 2023 Sep 26:105702. doi: 10.1016/j.tiv.2023.105702. Online ahead of print.ABSTRACTPermeability and partition coefficients of the skin barrier are important for assessing dermal absorption, bioavailability, and safety of cosmetics and medicine. We use the Potts and Guy equation to analyse the dependence of skin permeability on the hydrophobicity of permeants and highlight the significant differences in published datasets. Correlations of solute partition to skin are examined to understand the likely causes of the differences in the skin permeability datasets. Recently published permeability datasets show w...
Source: Toxicology in Vitro - September 28, 2023 Category: Toxicology Authors: Benjamin N Deacon Nicola Piasentin Qiong Cai Tao Chen Guoping Lian Source Type: research
Osthole protects H9c2 cardiomyocytes against trastuzumab-induced damage by enhancing autophagy through the p38MAPK/mTOR signaling pathway
Toxicol In Vitro. 2023 Sep 26:105704. doi: 10.1016/j.tiv.2023.105704. Online ahead of print.ABSTRACTTrastuzumab (TRZ) is a novel targeted anti-tumor agent that significantly improve the survival of patients with human epidermal growth factor receptor (HER2) positive breast cancer. However, its clinical application is limited due to the side effects of cardiotoxicity. Osthol (OST), a coumarin derivative isolated from Cnidium monnieri (L.) Cusson, has previously demonstrated cardioprotective effects. The aim of this study was to observe the protective effect of OST on TRZ-induced cardiomyocytes damage and to explore its pote...
Source: Toxicology in Vitro - September 28, 2023 Category: Toxicology Authors: Huan Hou Yaping Yang Rong Chen Zhiping Guo Source Type: research
An examination of published datasets of skin permeability and partition coefficients
Toxicol In Vitro. 2023 Sep 26:105702. doi: 10.1016/j.tiv.2023.105702. Online ahead of print.ABSTRACTPermeability and partition coefficients of the skin barrier are important for assessing dermal absorption, bioavailability, and safety of cosmetics and medicine. We use the Potts and Guy equation to analyse the dependence of skin permeability on the hydrophobicity of permeants and highlight the significant differences in published datasets. Correlations of solute partition to skin are examined to understand the likely causes of the differences in the skin permeability datasets. Recently published permeability datasets show w...
Source: Toxicology in Vitro - September 28, 2023 Category: Toxicology Authors: Benjamin N Deacon Nicola Piasentin Qiong Cai Tao Chen Guoping Lian Source Type: research
Salmonella mutant strains resistant to herbicides - Acetohydroxyacid synthase inhibitors and their use at the Ames test
Toxicol In Vitro. 2023 Sep 24:105699. doi: 10.1016/j.tiv.2023.105699. Online ahead of print.ABSTRACTCytotoxicity of some pesticides is a disadvantage for the Salmonella/microsome assay with regard to the equivalence assessment of pesticide technical grade active ingredients to the original products and detection of low-level impurities. The technical grade active ingredients (TGAIs) of pesticides from certain chemical classes were found to be toxic for Salmonella typhimurium strains. Among the highly cytotoxic compounds were sulfonylureas, which include 20 active ingredients. In addition, this class includes active pharmac...
Source: Toxicology in Vitro - September 26, 2023 Category: Toxicology Authors: Olga V Egorova Nataliya E Suzina Nataliya A Ilyushina Source Type: research
