Transcriptional landscape of human keratinocyte models exposed to 60-GHz millimeter-waves
Toxicol In Vitro. 2024 Mar 12;97:105808. doi: 10.1016/j.tiv.2024.105808. Online ahead of print.ABSTRACTThe use of millimeter waves (MMW) will exponentially grow in the coming years due to their future utilization in 5G/6G networks. The question of possible biological effects at these frequencies has been raised. In this present study, we aimed to investigate gene expression changes under exposure to MMW using the Bulk RNA Barcoding and sequencing (BRB-seq) technology. To address this issue, three exposure scenarios were performed aiming at: i) comparing the cellular response of two primary culture of keratinocytes (HEK and...
Source: Toxicology in Vitro - March 14, 2024 Category: Toxicology Authors: Catherine Martin Bertrand Evrard Fr édéric Percevault Kate Ryder Thomas Darde Aur élie Lardenois Maxim Zhadobov Ronan Sauleau Fr édéric Chalmel Yves Le Dr éan Denis Habauzit Source Type: research
Transcriptional landscape of human keratinocyte models exposed to 60-GHz millimeter-waves
Toxicol In Vitro. 2024 Mar 12:105808. doi: 10.1016/j.tiv.2024.105808. Online ahead of print.ABSTRACTThe use of millimeter waves (MMW) will exponentially grow in the coming years due to their future utilization in 5G/6G networks. The question of possible biological effects at these frequencies has been raised. In this present study, we aimed to investigate gene expression changes under exposure to MMW using the Bulk RNA Barcoding and sequencing (BRB-seq) technology. To address this issue, three exposure scenarios were performed aiming at: i) comparing the cellular response of two primary culture of keratinocytes (HEK and NH...
Source: Toxicology in Vitro - March 14, 2024 Category: Toxicology Authors: Catherine Martin Bertrand Evrard Frederic Percevault Kate Ryder Thomas Darde Aur élie Lardenois Maxim Zhadobov Ronan Sauleau Fr édéric Chalmel Yves Le Dr éan Denis Habauzit Source Type: research
Transcriptional landscape of human keratinocyte models exposed to 60-GHz millimeter-waves
Toxicol In Vitro. 2024 Mar 12:105808. doi: 10.1016/j.tiv.2024.105808. Online ahead of print.ABSTRACTThe use of millimeter waves (MMW) will exponentially grow in the coming years due to their future utilization in 5G/6G networks. The question of possible biological effects at these frequencies has been raised. In this present study, we aimed to investigate gene expression changes under exposure to MMW using the Bulk RNA Barcoding and sequencing (BRB-seq) technology. To address this issue, three exposure scenarios were performed aiming at: i) comparing the cellular response of two primary culture of keratinocytes (HEK and NH...
Source: Toxicology in Vitro - March 14, 2024 Category: Toxicology Authors: Catherine Martin Bertrand Evrard Frederic Percevault Kate Ryder Thomas Darde Aur élie Lardenois Maxim Zhadobov Ronan Sauleau Fr édéric Chalmel Yves Le Dr éan Denis Habauzit Source Type: research
Metformin and buparlisib synergistically induce apoptosis of non-small lung cancer (NSCLC) cells via Akt/FoxO3a/Puma axis
Toxicol In Vitro. 2024 Mar 11:105801. doi: 10.1016/j.tiv.2024.105801. Online ahead of print.ABSTRACTNon-small cell lung cancer (NSCLC) is a global health issue lacking effective treatments. Buparlisib is a pan-PI3K inhibitor that shows promising clinical results in treating NSCLC. However, chemoresistance is inevitable and hampers the application of buparlisib. Studies show that a combination of phytochemicals and chemotherapeutics enhances its effectiveness. Here, we evaluated the role of metformin, an agent with multiple pharmacological properties, in enhancing the anti-tumour activities of buparlisib against NSCLC cells...
Source: Toxicology in Vitro - March 13, 2024 Category: Toxicology Authors: Wang Shanshan Ma Hongying Fang Jingjing Yu Rui Source Type: research
Metformin and buparlisib synergistically induce apoptosis of non-small lung cancer (NSCLC) cells via Akt/FoxO3a/Puma axis
Toxicol In Vitro. 2024 Mar 11:105801. doi: 10.1016/j.tiv.2024.105801. Online ahead of print.ABSTRACTNon-small cell lung cancer (NSCLC) is a global health issue lacking effective treatments. Buparlisib is a pan-PI3K inhibitor that shows promising clinical results in treating NSCLC. However, chemoresistance is inevitable and hampers the application of buparlisib. Studies show that a combination of phytochemicals and chemotherapeutics enhances its effectiveness. Here, we evaluated the role of metformin, an agent with multiple pharmacological properties, in enhancing the anti-tumour activities of buparlisib against NSCLC cells...
Source: Toxicology in Vitro - March 13, 2024 Category: Toxicology Authors: Wang Shanshan Ma Hongying Fang Jingjing Yu Rui Source Type: research
In vitro assessment of cytotoxicity of spent fluid catalytic cracking refinery catalysts on cell lines and identification of critical toxic metals
Toxicol In Vitro. 2024 Mar 6;97:105807. doi: 10.1016/j.tiv.2024.105807. Online ahead of print.ABSTRACTThe Purpose of the present study was to quantify the responses of ten cell lines (HeLa, HepG2, HEK293, MDA-MB-231, A498, A549, A357, 3 T3, BALB-C3 T3, and NIH-3 T3) to spent fluid catalytic cracking catalysts (SFCCCs) from different petroleum refineries, and relate these responses to metal concentrations of SFCCC leachates (SFCCCLs). Cytotoxicity of SFCCCs were significantly different depending on cell lines. A357 and 3 T3 cell were the most sensitive, and A498 and HeLa cells were the least sensitive. HEK293 cells showed t...
Source: Toxicology in Vitro - March 8, 2024 Category: Toxicology Authors: Yue-Jie Wang Zhi-Guo Sheng Jun Li Zhen-Feng Zhao Ling-Ling Li Chen Shen Source Type: research
Simulated gastric leachate of 3D printer metal-fill filaments induces cytotoxic effects in rat and human intestinal models
This study investigated the cytotoxicity of extruded 3D printer filament leachates in rat and human intestinal cells. Copper-, bronze-, and steel-fill extruded filaments were incubated in acidic media for 2 h. Leachates were adjusted to pH 7 and cells exposed for 4 or 24 h. Concentration- and time-dependent decreases in rat and human cell viability were observed using a colorimetric assay and confirmed by microscopy. Copper- and bronze-fill leachates were more cytotoxic than steel. Copper-fill leachates had the highest copper concentrations by ICP-MS. Exposure to CuSO4 resulted in concentration-dependent cytotoxicity in ra...
Source: Toxicology in Vitro - March 8, 2024 Category: Toxicology Authors: Michael F Hughes Haley M Clapper Getachew Tedla Tyler D Sowers Kim R Rogers Source Type: research
In vitro assessment of cytotoxicity of spent fluid catalytic cracking refinery catalysts on cell lines and identification of critical toxic metals
Toxicol In Vitro. 2024 Mar 6:105807. doi: 10.1016/j.tiv.2024.105807. Online ahead of print.ABSTRACTThe Purpose of the present study was to quantify the responses of ten cell lines (HeLa, HepG2, HEK293, MDA-MB-231, A498, A549, A357, 3 T3, BALB-C3 T3, and NIH-3 T3) to spent fluid catalytic cracking catalysts (SFCCCs) from different petroleum refineries, and relate these responses to metal concentrations of SFCCC leachates (SFCCCLs). Cytotoxicity of SFCCCs were significantly different depending on cell lines. A357 and 3 T3 cell were the most sensitive, and A498 and HeLa cells were the least sensitive. HEK293 cells showed the ...
Source: Toxicology in Vitro - March 8, 2024 Category: Toxicology Authors: Yue-Jie Wang Zhi-Guo Sheng Jun Li Zhen-Feng Zhao Ling-Ling Li Chen Shen Source Type: research
Simulated gastric leachate of 3D printer metal-fill filaments induces cytotoxic effects in rat and human intestinal models
This study investigated the cytotoxicity of extruded 3D printer filament leachates in rat and human intestinal cells. Copper-, bronze-, and steel-fill extruded filaments were incubated in acidic media for 2 h. Leachates were adjusted to pH 7 and cells exposed for 4 or 24 h. Concentration- and time-dependent decreases in rat and human cell viability were observed using a colorimetric assay and confirmed by microscopy. Copper- and bronze-fill leachates were more cytotoxic than steel. Copper-fill leachates had the highest copper concentrations by ICP-MS. Exposure to CuSO4 resulted in concentration-dependent cytotoxicity in ra...
Source: Toxicology in Vitro - March 8, 2024 Category: Toxicology Authors: Michael F Hughes Haley M Clapper Getachew Tedla Tyler D Sowers Kim R Rogers Source Type: research
In vitro assessment of cytotoxicity of spent fluid catalytic cracking refinery catalysts on cell lines and identification of critical toxic metals
Toxicol In Vitro. 2024 Mar 6:105807. doi: 10.1016/j.tiv.2024.105807. Online ahead of print.ABSTRACTThe Purpose of the present study was to quantify the responses of ten cell lines (HeLa, HepG2, HEK293, MDA-MB-231, A498, A549, A357, 3 T3, BALB-C3 T3, and NIH-3 T3) to spent fluid catalytic cracking catalysts (SFCCCs) from different petroleum refineries, and relate these responses to metal concentrations of SFCCC leachates (SFCCCLs). Cytotoxicity of SFCCCs were significantly different depending on cell lines. A357 and 3 T3 cell were the most sensitive, and A498 and HeLa cells were the least sensitive. HEK293 cells showed the ...
Source: Toxicology in Vitro - March 8, 2024 Category: Toxicology Authors: Yue-Jie Wang Zhi-Guo Sheng Jun Li Zhen-Feng Zhao Ling-Ling Li Chen Shen Source Type: research
Simulated gastric leachate of 3D printer metal-fill filaments induces cytotoxic effects in rat and human intestinal models
This study investigated the cytotoxicity of extruded 3D printer filament leachates in rat and human intestinal cells. Copper-, bronze-, and steel-fill extruded filaments were incubated in acidic media for 2 h. Leachates were adjusted to pH 7 and cells exposed for 4 or 24 h. Concentration- and time-dependent decreases in rat and human cell viability were observed using a colorimetric assay and confirmed by microscopy. Copper- and bronze-fill leachates were more cytotoxic than steel. Copper-fill leachates had the highest copper concentrations by ICP-MS. Exposure to CuSO4 resulted in concentration-dependent cytotoxicity in ra...
Source: Toxicology in Vitro - March 8, 2024 Category: Toxicology Authors: Michael F Hughes Haley M Clapper Getachew Tedla Tyler D Sowers Kim R Rogers Source Type: research
In vitro assessment of cytotoxicity of spent fluid catalytic cracking refinery catalysts on cell lines and identification of critical toxic metals
Toxicol In Vitro. 2024 Mar 6:105807. doi: 10.1016/j.tiv.2024.105807. Online ahead of print.ABSTRACTThe Purpose of the present study was to quantify the responses of ten cell lines (HeLa, HepG2, HEK293, MDA-MB-231, A498, A549, A357, 3 T3, BALB-C3 T3, and NIH-3 T3) to spent fluid catalytic cracking catalysts (SFCCCs) from different petroleum refineries, and relate these responses to metal concentrations of SFCCC leachates (SFCCCLs). Cytotoxicity of SFCCCs were significantly different depending on cell lines. A357 and 3 T3 cell were the most sensitive, and A498 and HeLa cells were the least sensitive. HEK293 cells showed the ...
Source: Toxicology in Vitro - March 8, 2024 Category: Toxicology Authors: Yue-Jie Wang Zhi-Guo Sheng Jun Li Zhen-Feng Zhao Ling-Ling Li Chen Shen Source Type: research
Simulated gastric leachate of 3D printer metal-fill filaments induces cytotoxic effects in rat and human intestinal models
This study investigated the cytotoxicity of extruded 3D printer filament leachates in rat and human intestinal cells. Copper-, bronze-, and steel-fill extruded filaments were incubated in acidic media for 2 h. Leachates were adjusted to pH 7 and cells exposed for 4 or 24 h. Concentration- and time-dependent decreases in rat and human cell viability were observed using a colorimetric assay and confirmed by microscopy. Copper- and bronze-fill leachates were more cytotoxic than steel. Copper-fill leachates had the highest copper concentrations by ICP-MS. Exposure to CuSO4 resulted in concentration-dependent cytotoxicity in ra...
Source: Toxicology in Vitro - March 8, 2024 Category: Toxicology Authors: Michael F Hughes Haley M Clapper Getachew Tedla Tyler D Sowers Kim R Rogers Source Type: research
Transcriptomic profile of human iPSC-derived podocyte-like cells exposed to a panel of xenobiotics
Toxicol In Vitro. 2024 Mar 4:105804. doi: 10.1016/j.tiv.2024.105804. Online ahead of print.ABSTRACTPodocytes play a critical role in the formation and maintenance of the glomerular filtration barrier and injury to these cells can lead to a breakdown of the glomerular barrier causing permanent damage leading to progressive chronic kidney disease. Matured podocytes have little proliferative potential, which makes them critical cells from a health perspective, but also challenging cells to maintain in vitro. Differentiating podocyte-like cells from induced pluripotent stem cells (iPSC) cells provides a novel and continuous so...
Source: Toxicology in Vitro - March 6, 2024 Category: Toxicology Authors: Cormac Murphy Paul Jennings Anja Wilmes Source Type: research
Transcriptomic profile of human iPSC-derived podocyte-like cells exposed to a panel of xenobiotics
Toxicol In Vitro. 2024 Mar 4:105804. doi: 10.1016/j.tiv.2024.105804. Online ahead of print.ABSTRACTPodocytes play a critical role in the formation and maintenance of the glomerular filtration barrier and injury to these cells can lead to a breakdown of the glomerular barrier causing permanent damage leading to progressive chronic kidney disease. Matured podocytes have little proliferative potential, which makes them critical cells from a health perspective, but also challenging cells to maintain in vitro. Differentiating podocyte-like cells from induced pluripotent stem cells (iPSC) cells provides a novel and continuous so...
Source: Toxicology in Vitro - March 6, 2024 Category: Toxicology Authors: Cormac Murphy Paul Jennings Anja Wilmes Source Type: research
